Keratin 80 (KRT80) (NM_001081492) Human Untagged Clone

CAT#: SC315949

KRT80 (untagged)-Human keratin 80 (KRT80), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Keratin 80
• Synonyms: KB20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315949 representing NM_001081492.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCCTGCCGCTCCTGCGTGGTTGGCTTCAGCAGCCTCAGCAGCTGTGAGGTGACCCCGGTGGGCAGC
  CCCCGGCCTGGAACCTCAGGATGGGACAGCTGCAGGGCCCCCGGGCCGGGCTTCAGCTCCCGCAGCCTC
  ACAGGCTGCTGGTCGGCTGGCACTATCTCCAAGGTGACTGTGAACCCCGGCCTGCTGGTGCCCCTGGAT
  GTCAAGTTGGACCCCGCTGTTCAGCAGCTGAAGAACCAGGAGAAGGAGGAGATGAAGGCCCTCAATGAT
  AAATTTGCCTCCCTAATTGGCAAGGTGCAAGCCCTGGAACAGCGCAACCAGCTGCTGGAGACACGCTGG
  AGCTTCCTGCAGGGCCAGGACTCAGCCATCTTCGACCTCGGGCATCTCTATGAGGAATATCAGGGCCGG
  CTGCAGGAGGAACTGCGCAAAGTGAGCCAGGAGCGGGGGCAGCTGGAGGCCAACCTGCTGCAGGTGCTG
  GAGAAGGTTGAGGAGTTTCGAATCAGGTATGAGGATGAGATCTCCAAGCGCACAGACATGGAGTTCACC
  TTTGTTCAGCTGAAGAAGGACCTGGATGCAGAGTGTCTTCATCGGACTGAACTGGAAACCAAGTTAAAA
  AGCCTGGAGAGCTTCGTGGAGTTGATGAAAACCATCTATGAGCAGGAGCTGAAGGACCTGGCAGCACAG
  GTGAAGGATGTGTCGGTGACCGTCGGCATGGACAGCCGCTGCCACATCGACCTGAGCGGCATCGTGGAG
  GAGGTGAAGGCCCAGTATGACGCCGTCGCGGCTCGCAGCCTGGAGGAGGCCGAGGCATACTCTCGGAGC
  CAGCTGGAGGAGCAGGCCGCCCGCTCGGCCGAGTATGGGAGCAGCCTCCAGAGCAGCCGCAGCGAGATC
  GCGGATCTCAATGTGCGCATCCAGAAGCTGCGGTCCCAGATCCTCTCTGTCAAGAGCCATTGCCTGAAA
  CTGGAGGAGAACATCAAGACAGCTGAGGAGCAGGGTGAGCTGGCCTTCCAGGATGCCAAGACCAAGCTG
  GCCCAGCTGGAGGCCGCCCTGCAGCAGGCCAAGCAGGACATGGCGCGGCAGCTGCGCAAGTACCAGGAG
  CTGATGAACGTCAAGCTGGCCCTGGACATCGAGATCGCCACCTACAGGAAGCTGGTGGAGGGCGAGGAG
  GGCAGGATGGACTCGCCCTCAGCCACTGTGGTCAGCGCTGTGCAGTCCAGGTGCAAAACCGCCCCTTCC
  CTCCCCTACCCCTTATGTTCCCTGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001081492
• Insert Size: 1269 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001081492.1
• RefSeq Size: 3894 bp
• RefSeq ORF: 1269 bp
• Locus ID: 144501
• UniProt ID: Q6KB66
• Cytogenetics: 12q13.13
• MW: 47.2 kDa
• Gene Summary: Keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into epithelial keratins and hair keratins. This gene's expression profile shows that it encodes a type II epithelial keratin, although structurally the encoded protein is more like a type II hair keratin. This protein is involved in cell differentiation, localizing near desmosomal plaques in earlier stages of differentiation but then dispersing throughout the cytoplasm in terminally differentiating cells. The type II keratins are clustered in a region of chromosome 12q13. Two transcript variants encoding two different fully functional isoforms have been found for this gene.[provided by RefSeq, Oct 2010]
  Transcript Variant: This variant (2) differs in the 3' coding region and 3' UTR, compared to variant 1. The encoded isoform (K80.1, also known as b) has a distinct C-terminus and is shorter than isoform K80.