HSPA12A (NM_025015) Human Untagged Clone

CAT#: SC315821

HSPA12A (untagged)-Human heat shock 70kDa protein 12A (HSPA12A)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HSPA12A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315821 representing NM_025015.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGACAAGGAGGCCGGCGGCAGCGACGGGCCCCGAGAAACGGCTCCCACATCTGCATATTCATCT
  CCAGCCCGGAGTCTTGGGGACACAGGAATAACGCCTCTGTCCCCCTCCCATATTGTGAACGACACTGAC
  TCCAACGTCTCAGAACAGCAGTCATTTCTCGTGGTGGTGGCCGTCGACTTTGGGACCACATCCAGTGGC
  TATGCCTACAGCTTCACCAAGGAGCCGGAATGCATCCATGTGATGAGGCGATGGGAGGGAGGTGACCCT
  GGTGTGTCCAATCAGAAGACTCCAACCACCATCTTGCTGACTCCCGAGAGGAAGTTCCACAGCTTCGGG
  TATGCCGCCAGGGACTTTTACCATGACCTGGATCCCAATGAGGCCAAGCAGTGGCTGTACCTGGAGAAG
  TTCAAGATGAAGCTGCACACCACTGGGGACCTCACCATGGATACAGACCTGACGGCAGCAAATGGCAAG
  AAAGTCAAAGCCCTTGAAATCTTTGCTTATGCCCTGCAGTACTTTAAGGAGCAGGCGCTGAAGGAGCTG
  AGTGACCAGGCGGGTTCGGAGTTCGAGAACTCTGATGTCAGATGGGTCATCACGGTGCCTGCCATCTGG
  AAGCAGCCGGCCAAGCAGTTCATGAGACAAGCTGCCTACCAGGCAGGCCTGGCCTCCCCCGAGAACTCG
  GAGCAGCTCATCATTGCCTTGGAGCCTGAGGCAGCCTCTATCTACTGCCGAAAGCTGCGGCTACACCAG
  ATGATTGAGCTGAGCAGCAAGGCAGCCGTCAATGGGTACAGCGGCAGTGACACAGTAGGAGCTGGGTTT
  ACACAGGCTAAGGAACACATACGGCGTAATCGGCAGAGTCGGACCTTTTTGGTGGAGAATGTCATAGGA
  GAAATCTGGTCCGAGCTGGAGGAAGGTGATAAGTATGTGGTTGTGGACAGTGGCGGTGGCACCGTAGAC
  CTGACAGTCCATCAGATCCGGTTACCGGAGGGACACCTTAAGGAACTGTATAAAGCAACAGGCGGACCC
  TATGGATCTTTAGGAGTAGATTATGAGTTCGAAAAACTTCTGTATAAAATATTTGGAGAGGATTTTATT
  GAACAATTCAAAATCAAACGCCCTGCAGCCTGGGTTGACTTAATGATTGCGTTTGAGTCTCGCAAAAGG
  GCGGCTGCCCCAGACAGAACTAACCCGCTGAACATCACCCTGCCCTTCTCCTTCATTGACTACTACAAG
  AAGTTCCGCGGGCACAGTGTGGAGCACGCCTTGCGGAAAAGCAATGTGGATTTTGTGAAGTGGTCCTCG
  CAGGGGATGCTGCGGATGAGTCCAGATGCCATGAACGCCCTTTTTAAGCCGACCATCGATAGCATCATT
  GAGCATCTCCGGGACCTGTTTCAGAAGCCCGAGGTGTCCACCGTCAAGTTCCTCTTTCTGGTGGGCGGC
  TTTGCCGAGGCGCCCCTGCTGCAGCAGGCGGTGCAGGCTGCTTTTGGGGACCAGTGCCGGATCATCATC
  CCCCAGGACGTGGGCCTCACCATCCTCAAGGGTGCCGTCCTCTTTGGCCTGGACCCCGCGGTCATCAAG
  GTGCGCCGGTCGCCGCTCACCTACGGGGTAGGCGTGCTGAACCGCTACGTGGAGGGCAAGCACCCGCCT
  GAGAAGCTGCTGGTGAAGGATGGCACTCGGTGGTGCACCGACGTCTTTGACAAGTTCATCTCTGCCGAC
  CAGTCTGTGGCTCTGGGTGAGCTGGTCAAGCGTAGCTACACCCCGGCCAAGCCCTCCCAGCTGGTCATT
  GTCATCAACATCTACAGCTCTGAGCACGACAACGTCAGCTTCATCACTGATCCCGGGGTGAAGAAGTGT
  GGCACGCTCCGCCTGGATCTCACAGGGACCAGTGGCACTGCGGTGCCCGCCCGGAGGGAGATCCAGACC
  CTTATGCAGTTCGGGGACACCGAGATCAAAGCCACAGCCATTGATATAGCCACTTCGAAGAGTGTCAAA
  GTTGGGATCGACTTCTTAAATTACTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_025015
• Insert Size: 2028 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025015.2
• RefSeq Size: 5739 bp
• RefSeq ORF: 2028 bp
• Locus ID: 259217
• UniProt ID: O43301
• Cytogenetics: 10q25.3
• MW: 75 kDa
• Gene Summary: Adapter protein for SORL1, but not SORT1. Delays SORL1 internalization and affects SORL1 subcellular localization.[UniProtKB/Swiss-Prot Function]