ACSM4 (NM_001080454) Human Untagged Clone

CAT#: SC315804

ACSM4 (untagged)-Human acyl-CoA synthetase medium-chain family member 4 (ACSM4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ACSM4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315804 representing NM_001080454.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGATTTTTTTCCGCTACCAGACATTTAGATTCATCTGGCTCACCAAGCCACCTGGCCGGCGCTTA
  CACAAAGATCACCAGCTTTGGACGCCTCTGACTCTTGCTGACTTTGAAGCCATAAATCGCTGTAACAGG
  CCATTGCCTAAAAACTTTAACTTTGCTGCAGATGTGCTGGACCAGTGGTCCCAAAAGGAGAAGACAGGG
  GAGAGACCAGCTAACCCAGCCCTGTGGTGGGTGAATGGCAAAGGGGATGAGGTAAAATGGAGCTTCAGA
  GAACTGGGCTCCTTGTCCCGAAAAGCTGCCAACGTGCTCACCAAGCCCTGTGGCCTGCAGAGAGGAGAC
  CGTTTGGCCGTGATTCTGCCCAGAATCCCTGAGTGGTGGCTGGTCAATGTAGCTTGCATACGAACAGGG
  ATCATCTTCATGCCGGGAACAATCCAGCTGACAGCAAAAGACATCCTCTACCGGCTGCGAGCATCCAAG
  GCCAAGTGCATTGTGGCCAGTGAGGAGGTGGCCCCAGCGGTGGAGTCCATTGTATTGGAGTGTCCTGAC
  CTTAAGACAAAACTCCTGGTGTCTCCACAAAGCTGGAATGGGTGGCTCAGCTTCCAGGAGTTATTTCAA
  TTCGCCTCTGAAGAGCACAGCTGTGTGGAAACAGGAAGTCAAGAACCAATGACCATTTATTTCACCAGT
  GGGACCACAGGCTTTCCTAAAATGGCCCAGCACTCTCAGAGCAGCCTCGGCATTGGGTTCACCCTCTGC
  GGAAGGTATTGGCTGGACTTGAAGTCCTCAGATATCATATGGAATATGTCTGACACGGGCTGGGTCAAG
  GCCGCCATTGGCAGTGTGTTTTCTTCCTGGCTGTGTGGAGCCTGTGTTTTTGTGCATCGAATGGCACAG
  TTTGACACTGACACCTTCCTAGACACACTTACTACTTATCCCATCACGACCCTGTGCAGTCCTCCCACT
  GTGTACCGGATGCTCGTGCAAAAAGACCTTAAGAGATATAAATTCAAGAGTCTGCGGCACTGCTTGACC
  GGAGGGGAGCCACTCAACCCAGAAGTGCTGGAGCAGTGGAGGGTGCAAACTGGGCTGGAGCTATATGAG
  GGCTATGGACAGACGGAAGTGGGAATGATTTGTGCCAATCAGAAAGGCCAAGAAATTAAACCAGGTTCA
  ATGGGGAAAGGAATGCTGCCCTATGATGTCCAGATTATAGATGAAAATGGCAATGTTCTACCACCTGGC
  AAAGAAGGGGAAATTGCCCTCAGACTCAAACCTACACGGCCCTTCTGTTTCTTCTCTAAATATGTGGAC
  AATCCACAGAAAACTGCTGCCACGATAAGAGGAGATTTTTATGTCACTGGAGACAGAGGAGTGATGGAC
  AGTGATGGGTATTTCTGGTTTGTCGGCAGAGCTGATGATGTCATTATATCCTCTGGGTACCGTATTGGG
  CCATTTGAAGTGGAGAGTGCACTCATTGAGCATCCAGCAGTTGTTGAATCGGCTGTTGTCAGTAGTCCA
  GATCAAATCCGCGGAGAGGTGGTGAAAGCTTTTGTTGTCTTAGCTGCACCCTTTAAGTCCTACAACCCA
  GAGAAATTAACTCTTGAACTTCAGGATCATGTGAAAAAATCAACTGCACCTTACAAATATCCAAGAAAG
  GTGGAATTTGTTCAAGAACTCCCAAAGACAATCACTGGGAAAATCAAACGCAACGTTTTAAGAGACCAA
  GAATGGAGAGGAAGATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001080454
• Insert Size: 1743 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001080454.1
• RefSeq Size: 1743 bp
• RefSeq ORF: 1743 bp
• Locus ID: 341392
• UniProt ID: P0C7M7
• Cytogenetics: 12p13.31
• Protein Pathways: Butanoate metabolism, Metabolic pathways
• MW: 65.7 kDa
• Gene Summary: Has medium-chain fatty acid:CoA ligase activity with broad substrate specificity (in vitro). Acts on acids from C(4) to C(11) and on the corresponding 3-hydroxy- and 2,3- or 3,4-unsaturated acids (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]