ASPP1 (PPP1R13B) (NM_015316) Human Untagged Clone

CAT#: SC315169

PPP1R13B (untagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ASPP1
• Synonyms: ASPP1; p53BP2-like; p85
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC315169 representing NM_015316.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGATGCCGATGATATTAACTGTTTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACA
  CCGGAAACAACCTGTCGAGATGTTGTAGAATTTTGCAAGGAACCTGGAGAAGGCAGCTGCCATTTAGCT
  GAAGTGTGGAGGGGAAATGAACGTCCCATACCCTTTGATCATATGATGTACGAACATCTTCAGAAATGG
  GGTCCACGGAGGGAAGAAGTGAAATTTTTCCTTCGACACGAGGACTCCCCAACTGAGAACAGTGAACAA
  GGTGGCCGTCAGACCCAAGAGCAACGAACTCAGAGAAATGTAATAAATGTACCTGGAGAAAAACGTACT
  GAAAATGGGGTTGGGAATCCACGTGTTGAACTTACCCTCTCAGAGCTCCAAGATATGGCAGCTAGGCAA
  CAGCAGCAGATTGAAAATCAGCAGCAGATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAGCAA
  CAGGAGCGCCGTCAGCAGCAGTCTATTTCTGAAAATGAAAAGCTTCAGAAATTGAAAGAACGAGTTGAA
  GCCCAGGAGAACAAGCTGAAGAAAATTCGTGCAATGAGAGGACAAGTCGACTACAGCAAAATCATGAAC
  GGCAATCTGTCTGCTGAAATAGAAAGGTTCAGTGCCATGTTCCAGGAAAAGAAGCAGGAAGTACAGACT
  GCAATTTTAAGGGTTGATCAGCTTAGTCAGCAATTGGAAGATTTAAAGAAAGGAAAACTGAATGGGTTC
  CAGTCTTACAATGGCAAATTGACGGGACCAGCGGCGGTGGAGTTAAAAAGACTGTACCAAGAACTACAG
  ATTCGTAACCAACTTAACCAGGAACAAAATTCAAAACTTCAGCAGCAGAAGGAACTCTTAAATAAGCGC
  AACATGGAGGTGGCCATGATGGACAAGCGAATCAGTGAACTGCGTGAACGTCTCTATGGGAAAAAAATT
  CAGCTGAACCGTGTGAATGGCACGTCATCACCACAGTCCCCTCTGAGCACATCGGGCAGGGTCGCTGCT
  GTGGGGCCTTATATCCAGGTTCCCAGTGCCGGAAGCTTTCCTGTGCTGGGGGACCCTATAAAGCCCCAG
  TCTCTCAGTATTGCCTCAAATGCTGCTCATGGAAGATCCAAATCCGCTAATGATGGAAACTGGCCAACA
  TTAAAACAGAATTCTAGCTCTTCCGTGAAACCAGTGCAGGTGGCCGGTGCAGACTGGAAGGATCCGAGC
  GTGGAGGGGTCTGTCAAGCAGGGCACTGTCTCCAGCCAGCCTGTGCCCTTCTCAGCACTGGGACCCACG
  GAGAAGCCGGGCATCGAGATTGGTAAAGTGCCACCTCCCATCCCGGGTGTAGGCAAGCAGCTGCCTCCA
  AGCTATGGGACATACCCAAGTCCTACACCTCTGGGTCCTGGGTCGACAAGCTCCCTGGAAAGGAGGAAG
  GAAGGCAGCTTGCCCAGGCCCAGTGCAGGCCTGCCAAGTCGACAGAGGCCCACCCTGCTGCCCGCCACA
  GGCAGCACCCCCCAGCCAGGCTCCTCACAACAGATTCAGCAGAGGATTTCCGTACCGCCAAGTCCCACG
  TACCCGCCAGCGGGACCACCTGCATTTCCAGCTGGGGACAGCAAGCCTGAACTCCCACTGACAGTGGCC
  ATTAGGCCTTTCCTGGCTGATAAAGGGTCAAGGCCACAGTCTCCCAGGAAAGGACCCCAGACAGTGAAT
  TCAAGTTCCATATACTCCATGTACCTCCAGCAAGCCACACCACCTAAGAATTACCAGCCGGCAGCACAC
  AGCGCCTTAAATAAGTCAGTTAAAGCAGTGTATGGTAAGCCCGTTTTACCTTCGGGTTCAACCTCTCCA
  TCGCCGCTGCCGTTTCTTCACGGGTCACTGTCCACGGGCACACCACAGCCTCAGCCACCTTCAGAAAGT
  ACTGAGAAAGAGCCTGAGCAGGATGGCCCCGCCGCCCCCGCAGATGGCAGCACCGTGGAGAGCCTGCCA
  CGGCCACTCAGCCCCACCAAGCTCACGCCCATCGTGCATTCGCCACTGCGCTACCAGAGTGATGCAGAC
  CTGGAGGCCCTCCGCAGGAAGCTGGCCAACGCGCCCCGGCCCCTGAAAAAGCGCAGCTCCATCACAGAG
  CCCGAGGGCCCCGGCGGGCCCAACATCCAGAAGCTGCTGTACCAGCGCTTCAACACCCTGGCCGGTGGC
  ATGGAGGGCACCCCTTTCTACCAGCCCAGCCCCTCCCAGGACTTCATGGGCACCTTGGCCGATGTGGAC
  AATGGAAACACCAATGCCAATGGAAACCTGGAAGAGCTCCCCCCTGCCCAGCCCACAGCCCCACTCCCC
  GCTGAGCCTGCCCCGTCATCAGATGCCAATGATAATGAGTTACCTTCCCCCGAACCAGAGGAGCTCATC
  TGTCCCCAAACCACCCACCAAACTGCCGAGCCGGCAGAGGACAATAACAACAACGTGGCCACGGTCCCC
  ACCACGGAGCAGATCCCGAGTCCTGTGGCTGAGGCCCCATCTCCAGGGGAAGAGCAGGTCCCTCCAGCA
  CCTCTTCCCCCTGCCAGCCACCCTCCTGCCACCTCCACGAACAAGCGGACCAACTTGAAGAAGCCCAAC
  TCGGAGCGGACGGGGCACGGGCTGAGAGTCCGGTTTAACCCCCTGGCACTGCTCCTAGACGCGTCTCTG
  GAAGGAGAGTTCGATCTGGTGCAGAGGATCATCTATGAGGTGGAAGATCCCAGCAAGCCCAACGACGAA
  GGGATCACCCCACTGCACAACGCCGTCTGCGCCGGCCACCATCACATCGTGAAGTTCCTGCTGGATTTT
  GGTGTCAACGTGAATGCTGCTGATAGTGATGGATGGACGCCGCTGCACTGCGCTGCCTCTTGTAACAGC
  GTTCACCTCTGCAAACAGCTGGTGGAGAGTGGTGCCGCCATTTTTGCCTCAACCATAAGCGACATTGAA
  ACTGCTGCAGACAAGTGTGAGGAGATGGAGGAAGGCTACATCCAGTGCTCCCAGTTTCTATATGGGGTG
  CAGGAAAAGCTGGGTGTGATGAACAAAGGTGTGGCGTATGCTCTGTGGGACTACGAGGCCCAGAACAGT
  GACGAGCTGTCCTTCCACGAAGGGGACGCCCTCACCATCCTGAGGCGCAAGGACGAAAGCGAGACTGAG
  TGGTGGTGGGCTCGCCTTGGAGACCGGGAGGGCTATGTGCCCAAAAACCTGCTGGGGCTGTATCCACGG
  ATCAAACCCCGACAGCGAACACTCGCCTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_015316
• Insert Size: 3273 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015316.1
• RefSeq Size: 4976 bp
• RefSeq ORF: 3273 bp
• Locus ID: 23368
• UniProt ID: Q96KQ4
• Cytogenetics: 14q32.33
• Domains: SH3, ANK
• Protein Families: Druggable Genome
• MW: 119.6 kDa
• Gene Summary: This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]