ASPP1 (PPP1R13B) (NM_015316) Human Tagged ORF Clone

CAT#: RG221172

PPP1R13B (tGFP-tagged) - Human protein phosphatase 1, regulatory (inhibitor) subunit 13B (PPP1R13B)

Product Images

Circular map for RG221172

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ASPP1
• Synonyms: ASPP1; p53BP2-like; p85
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG221172 representing NM_015316
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGATGCCGATGATATTAACTGTTTTCTTGAGCAACAATGAACAGATTTTAACAGAAGTTCCTATAACAC
  CGGAAACAACCTGTCGAGATGTTGTAGAATTTTGCAAGGAACCTGGAGAAGGCAGCTGCCATTTAGCTGA
  AGTGTGGAGGGGAAATGAACGTCCCATACCCTTTGATCATATGATGTACGAACATCTTCAGATATGGGGT
  CCACGGAGGGAAGAAGTGAAATTTTTCCTTCGACACGAGGACTCCCCAACTGAGAACAGTGAACAAGGTG
  GCCGTCAGACCCAAGAGCAACGAACTCAGAGAAATGTAATAAATGTACCTGGAGATAAACGTACTGAATA
  TGGGGTTGGGAATCCACGTGTTGAACTTACCCTCTCAGAGCTCCAAGATATGGCAGCTAGGCAACAGCAG
  CAGATTGAAAATCAGCAGCAGATGTTGGTTGCCAAGGAACAGCGTTTACATTTTCTAAAGCAACAGGAGC
  GCCGTCAGCAGCAGTCTATTTCTGAAAATGAAAAGCTTCAGAAATTGAAAGAACGAGTTGAAGCCCAGGA
  GAACAAGCTGAAGAAAATTCGTGCAATGAGAGGACAAGTCGACTACAGCAAAATCATGAACGGCAATCTG
  TCTGCTGAAATAGAAAGGTTCAGTGCCATGTTCCAGGAAAAGAAGCAGGAAGTACAGACTGCAATTTTAA
  GGGTTGATCAGCTTAGTCAGCAATTGGAAGATTTAAAGAAAGGAAAACTGAATGGGTTCCAGTCTTACAA
  TGGCAAATTGACGGGACCAGCGGCGGTGGAGTTAAAAAGACTGTACCAAGAACTACAGATTCGTAACCAA
  CTTAACCAGGAACAAAATTCAAAACTTCAGCAGCAGAAGGAACTCTTAAATAAGCGCAACATGGAGGTGG
  CCATGATGGACAAGCGAATCAGTGAACTGCGTGAACGTCTCTATGGGAAAAAAATTCAGCTGAACCGTGT
  GAATGGCACGTCATCACCACAGTCCCCTCTGAGCACATCGGGCAGGGTCGCTGCTGTGGGGCCTTATATC
  CAGGTTCCCAGTGCCGGAAGCTTTCCTGTGCTGGGGGACCCTATAAAGCCCCAGTCTCTCAGTATTGCCT
  CAAATGCTGCTCATGGAAGATCCAAATCCGCTAATGATGGAAACTGGCCAACATTAAAACAGAATTCTAG
  CTCTTCCGTGAAACCAGTGCAGGTGGCCGGTGCAGACTGGAAGGATCCGAGCGTGGAGGGGTCTGTCAAG
  CAGGGCACTGTCTCCAGCCAGCCTGTGCCCTTCTCAGCACTGGGACCCACGGAGAAGCCGGGCATCGAGA
  TTGGTAAAGTGCCACCTCCCATCCCGGGTGTAGGCAAGCAGCTGCCTCCAAGCTATGGGACATACCCAAG
  TCCTACACCTCTGGGTCCTGGGTCGACAAGCTCCCTGGAAAGGAGGAAGGAAGGCAGCTTGCCCAGGCCC
  AGTGCAGGCCTGCCAAGTCGACAGAGGCCCACCCTGCTGCCCGCCACAGGCAGCACCCCCCAGCCAGGCT
  CCTCACAACAGATTCAGCAGAGGATTTCCGTACCGCCAAGTCCCACGTACCCGCCAGCGGGACCACCTGC
  ATTTCCAGCTGGGGACAGCAAGCCTGAACTCCCACTGACAGTGGCCATTAGGCCTTTCCTGGCTGATAAA
  GGGTCAAGGCCACAGTCTCCCAGGAAAGGACCCCAGACAGTGAATTCAAGTTCCATATACTCCATGTACC
  TCCAGCAAGCCACACCACCTAAGAATTACCAGCCGGCAGCACACAGCGCCTTAAATAAGTCAGTTAAAGC
  AGTGTATGGTAAGCCCGTTTTACCTTCGGGTTCAACCTCTCCATCGCCGCTGCCGTTTCTTCACGGGTCA
  CTGTCCACGGGCACACCACAGCCTCAGCCACCTTCAGAAAGTACTGAGAAAGAGCCTGAGCAGGATGGCC
  CCGCCGCCCCCGCAGATGGCAGCACCGTGGAGAGCCTGCCACGGCCACTCAGCCCCACCAAGCTCACGCC
  CATCGTGCATTCGCCACTGCGCTACCAGAGTGATGCAGACCTGGAGGCCCTCCGCAGGAAGCTGGCCAAC
  GCGCCCCGGCCCCTGAAAAAGCGCAGCTCCATCACAGAGCCCGAGGGCCCCGGCGGGCCCAACATCCAGA
  AGCTGCTGTACCAGCGCTTCAACACCCTGGCCGGTGGCATGGAGGGCACCCCTTTCTACCAGCCCAGCCC
  CTCCCAGGACTTCATGGGCACCTTGGCCGATGTGGACAATGGAAACACCAATGCCAATGGAAACCTGGAA
  GAGCTCCCCCCTGCCCAGCCCACAGCCCCACTCCCCGCTGAGCCTGCCCCGTCATCAGATGCCAATGATA
  ATGAGTTACCTTCCCCCGAACCAGAGGAGCTCATCTGTCCCCAAACCACCCACCAAACTGCCGAGCCGGC
  AGAGGACAATAACAACAACGTGGCCACGGTCCCCACCACGGAGCAGATCCCGAGTCCTGTGGCTGAGGCC
  CCATCTCCAGGGGAAGAGCAGGTCCCTCCAGCACCTCTTCCCCCTGCCAGCCACCCTCCTGCCACCTCCA
  CGAACAAGCGGACCAACTTGAAGAAGCCCAACTCGGAGCGGACGGGGCACGGGCTGAGAGTCCGGTTTAA
  CCCCCTGGCACTGCTCCTAGACGCGTCTCTGGAAGGAGAGTTCGATCTGGTGCAGAGGATCATCTATGAG
  GTGGAAGATCCCAGCAAGCCCAACGATGAAGGGATCACCCCACTGCACAACGCCGTCTGCGCCGGCCACC
  ATCACATCGTGAAGTTCCTGCTGGATTTTGGTGTCAACGTGAATGCTGCTGATAGTGATGGATGGACGCC
  GCTGCACTGCGCTGCCTCTTGTAACAGCGTTCACCTCTGCAAACAGCTGGTGGAGAGTGGTGCCGCCATT
  TTTGCCTCAACCATAAGCGACATTGAAACTGCTGCAGACAAGTGTGAGGAGATGGAGGAAGGCTACATCC
  AGTGCTCCCAGTTTCTATATGGGGTGCAGGAAAAGCTGGGTGTGATGAACAAAGGTGTGGCGTATGCTCT
  GTGGGACTACGAGGCCCAGAACAGTGACGAGCTGTCCTTCCACGAAGGGGACGCCCTCACCATCCTGAGG
  CGCAAGGACGAAAGCGAGACTGAGTGGTGGTGGGCTCGCCTTGGAGACCGGGAGGGCTATGTGCCCAAAA
  ACCTGCTGGGGCTGTATCCACGGATCAAACCCCGACAGCGAACACTCGCC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG221172 representing NM_015316
  Red=Cloning site Green=Tags(s)
  
  MMPMILTVFLSNNEQILTEVPITPETTCRDVVEFCKEPGEGSCHLAEVWRGNERPIPFDHMMYEHLQIWG
  PRREEVKFFLRHEDSPTENSEQGGRQTQEQRTQRNVINVPGDKRTEYGVGNPRVELTLSELQDMAARQQQ
  QIENQQQMLVAKEQRLHFLKQQERRQQQSISENEKLQKLKERVEAQENKLKKIRAMRGQVDYSKIMNGNL
  SAEIERFSAMFQEKKQEVQTAILRVDQLSQQLEDLKKGKLNGFQSYNGKLTGPAAVELKRLYQELQIRNQ
  LNQEQNSKLQQQKELLNKRNMEVAMMDKRISELRERLYGKKIQLNRVNGTSSPQSPLSTSGRVAAVGPYI
  QVPSAGSFPVLGDPIKPQSLSIASNAAHGRSKSANDGNWPTLKQNSSSSVKPVQVAGADWKDPSVEGSVK
  QGTVSSQPVPFSALGPTEKPGIEIGKVPPPIPGVGKQLPPSYGTYPSPTPLGPGSTSSLERRKEGSLPRP
  SAGLPSRQRPTLLPATGSTPQPGSSQQIQQRISVPPSPTYPPAGPPAFPAGDSKPELPLTVAIRPFLADK
  GSRPQSPRKGPQTVNSSSIYSMYLQQATPPKNYQPAAHSALNKSVKAVYGKPVLPSGSTSPSPLPFLHGS
  LSTGTPQPQPPSESTEKEPEQDGPAAPADGSTVESLPRPLSPTKLTPIVHSPLRYQSDADLEALRRKLAN
  APRPLKKRSSITEPEGPGGPNIQKLLYQRFNTLAGGMEGTPFYQPSPSQDFMGTLADVDNGNTNANGNLE
  ELPPAQPTAPLPAEPAPSSDANDNELPSPEPEELICPQTTHQTAEPAEDNNNNVATVPTTEQIPSPVAEA
  PSPGEEQVPPAPLPPASHPPATSTNKRTNLKKPNSERTGHGLRVRFNPLALLLDASLEGEFDLVQRIIYE
  VEDPSKPNDEGITPLHNAVCAGHHHIVKFLLDFGVNVNAADSDGWTPLHCAASCNSVHLCKQLVESGAAI
  FASTISDIETAADKCEEMEEGYIQCSQFLYGVQEKLGVMNKGVAYALWDYEAQNSDELSFHEGDALTILR
  RKDESETEWWWARLGDREGYVPKNLLGLYPRIKPRQRTLA
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015316
• ORF Size: 3270 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015316.1, NP_056131.1
• RefSeq Size: 4834 bp
• RefSeq ORF: 3273 bp
• Locus ID: 23368
• UniProt ID: Q96KQ4
• Cytogenetics: 14q32.33
• Domains: SH3, ANK
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the ASPP (apoptosis-stimulating protein of p53) family of p53 interacting proteins. The protein contains four ankyrin repeats and an SH3 domain involved in protein-protein interactions. ASPP proteins are required for the induction of apoptosis by p53-family proteins. They promote DNA binding and transactivation of p53-family proteins on the promoters of proapoptotic genes. Expression of this gene is regulated by the E2F transcription factor. [provided by RefSeq, Jul 2008]