PCDHA4 (NM_018907) Human Untagged Clone

CAT#: SC314971

PCDHA4 (untagged)-Human protocadherin alpha 4 (PCDHA4), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PCDHA4
• Synonyms: CNR1; CNRN1; CRNR1; PCDH-ALPHA4
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_018907 edited
  ATGGAGTTTTCCTGGGGAAGCGGCCAGGAATCCCGGCGTCTGCTGCTCTTACTTCTTCTC
  CTCGCAGCCTGGGAGGCAGGGAACGGTCAGCTCCACTACTCGGTCTCCGAGGAGGCCAAA
  CACGGCACCTTCGTGGGCCGCATCGCGCAGGACCTGGGACTGGAGCTGGCGGAGCTGGTG
  CCGCGCCTGTTCCGGGTGGCGTCCAAGGGCCGCGGAGGCCTTCTGGAGGTAAATCTGCAG
  AATGGCATTTTGTTTGTGAATTCTCGGATCGACCGGGAGGAGCTGTGCCGGCGGAGCGCG
  GAGTGCAGCATCCACCTGGAGGTGATCGTAGACAGGCCGCTGCAGGTTTTCCATGTGGAC
  GTGGAGGTGAGGGACATTAACGATAACCCGCCGGTGTTCCCAGCAACACAAAAGAACCTG
  TCCATCGCGGAATCCAGGCCGCTTGACTCTCGGTTTCCACTAGAGGGCGCCTCGGATGCA
  GATATCGGGGAGAACGCCCTGCTCACTTACAGACTGAGCCCAAATGAATACTTTTCTCTG
  GAAAAACCACCTGATGACGAGCTGGTAAAAGGTCTTGGGCTTATATTACGGAAATCTTTA
  GACAGAGAAGAAGCTCCGGAGATTTTTTTAGTGCTCACAGCCACTGATGGAGGCAAACCC
  GAGTTGACTGGCACCGTTCAGTTACTCATCACAGTACTGGATGCCAATGACAATGCCCCA
  GCTTTTGACAGAACCATTTATAAGGTGAGATTACTAGAAAATGTTCCTAATGGAACATTG
  GTAATTAAACTTAACGCCTCAGATTTAGACGAAGGATTGAATGGGGACATTGTTTATTCA
  TTCTCAAATGATATTTCGCCAAATGTGAAATCCAAGTTTCACATAGATCCAATTACTGGA
  CAAATTATTGTAAAGGGATATATTGACTTTGAAGAAAGCAAATCCTATGAAATTATTGTA
  GAGGGCATTGATAAGGGACAGCTCCCACTTTCTGGCCATTGTAGAGTTATTGTGGAAGTA
  GAAGACAACAACGATAATGTCCCAGATTTGGAATTCAAGTCTTTATCACTTCCAATTAGA
  GAGGACGCTCCACTGGGTACAGTCATCGCCCTGATCAGCGTGTCCGACAAAGACATGGGT
  GTCAATGGGCTGGTCACCTGCTCCTTGACGTCCCACGTCCCCTTCAAGCTGGTGTCCACC
  TTCAAGAATTACTACTCGTTGGTGCTGGACAGTGCCCTGGACCGCGAGAGCGTGTCAGCC
  TATGAGCTGGTGGTGACCGCGCGAGACGGGGGCTCGCCTTCGCTGTGGGCCACGGCCAGT
  GTTTCTGTGGAGGTGGCTGATGTGAACGACAACGCTCCGGCGTTCGCGCAGCCCGAGTAC
  ACAGTGTTCGTGAAGGAGAACAACCCGCCGGGCTGCCACATCTTCACTGTGTCTGCGTGG
  GACGCGGACGCGCAGGAGAACGCGCTGGTGTCCTACTCGCTGGTAGAGCGGCGGGTAGGG
  GAGCGCGCGCTGTCGAGCTACGTTTCGGTGCATGCGGAGAGCGGCAAGGTGTACGCGCTG
  CAGCCGCTGGACCACGAGGAGCTAGAGCTGCTGCAGTTTCAGGTGACCGCTCGCGATGCC
  GGCGTGCCACCTCTGGGCAGCAACGTGACGCTGCAGGTGTTCGTGCTGGACGAAAACGAC
  AACGCGCCAGCACTGCTAGCGCCTCGGGCGGGTGGCACTGGTGGCGCAGTGAGCGAGCTG
  GTGCCATGGTCGGTGGGTGTGGGCCACGTGGTGGCAAAGGTGCGCGCGGTGGATGCTGAC
  TCGGGCTACAACGCGTGGCTTTCGTACGAGCTGCAGCCGGGGACTGGTGGCGCGCGCATC
  CCGTTCCGCGTGGGGCTGTACACTGGCGAGATCAGCACAACGCGTGCCCTGGACGAAACG
  GACGCTCCGCGCCACCGCCTACTGGTACTGGTGAAGGACCACGGCGAGCCCGCGCTGACG
  GCCACGGCCACTGTGCTGGTGTCACTTGTGGAGAGTGGACAGGCGCCAAAGGCCTCCTCA
  CGGGCGTTGGTGGGCGCTGTGGGTCCCGATGCTGCGCTGGTGGATGTCAACGTATACCTG
  ATCATTGCCATCTGCGCGGTGTCCAGCCTTTTGGTGCTCACGCTGCTGCTGTACACCGCG
  CTGCGGTGCTCTGCGCTGCCCACCGAGGGCGCGTGCGCTCCGGGCAAGCCCACGCTGGTG
  TGCTCCAGTGCGGTGGGGAGCTGGTCATACTCGCAGCAGAGGAGGCCGAGGGTGTGCTCT
  GGTGAGGGCCCACCCAAGACCGACCTCATGGCCTTCAGCCCCAGTTTACCTGACTCTAGG
  GACAGAGAAGATCAGCTGCAGACAACTGAGGAATCCTTTGCAAAGCCACGACAGCCCAAC
  CCTGACTGGCGTTACTCTGCCTCCCTGAGAGCAGGCATGCACAGCTCTGTGCACCTAGAG
  GAGGCTGGCATTCTACGGGCTGGTCCAGGAGGGCCTGATCAGCAGTGGCCAACAGTATCC
  AGTGCAACACCAGAACCAGAGGCAGGAGAAGTGTCCCCTCCAGTCGGTGCGGGTGTCAAC
  AGCAACAGCTGGACCTTTAAATACGGACCAGGCAACCCCAAACAATCCGGTCCCGGTGAG
  TTGCCCGACAAATTCATTATCCCAGGATCTCCTGCAATCATCTCCATCCGGCAGGAGCCT
  ACTAACAGCCAAATTGACAAAAGTGACTTCATAACCTTCGGCAAAAAGGAGGAGACCAAG
  AAAAAGAAGAAAAAGAAGAAGGGTAACAAGACCCAGGAGAAAAAAGAGAAAGGGAACAGC
  ACGACTGACAACAGTGACCAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_018907
• Insert Size: 2900 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018907.2, NP_061730.1
• RefSeq Size: 5352 bp
• RefSeq ORF: 2844 bp
• Locus ID: 56144
• UniProt ID: Q9UN74
• Cytogenetics: 5q31.3
• Domains: CA
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin alpha gene cluster, one of three related gene clusters tandemly linked on chromosome five that demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The alpha gene cluster is composed of 15 cadherin superfamily genes related to the mouse CNR genes and consists of 13 highly similar and 2 more distantly related coding sequences. The tandem array of 15 N-terminal exons, or variable exons, are followed by downstream C-terminal exons, or constant exons, which are shared by all genes in the cluster. The large, uninterrupted N-terminal exons each encode six cadherin ectodomains while the C-terminal exons encode the cytoplasmic domain. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins that most likely play a critical role in the establishment and function of specific cell-cell connections in the brain. Alternative splicing has been observed and additional variants have been suggested but their full-length nature has yet to be determined. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) includes the constant region exons and encodes the longest isoform (1).