RFX2 (NM_134433) Human Untagged Clone

CAT#: SC314460

RFX2 (untagged)-Human regulatory factor X, 2 (influences HLA class II expression) (RFX2), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RFX2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC314460 representing NM_134433.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGAATTCCGAGGGTGGAGCGGATTCGCCAGCGTCCGTGGCTCTGCGTCCCTCGGCGGCAGCCCCG
  CCTGTGCCAGCCTCCCCGCAGAGGGTGTTGGTCCAGGCAGCCAGCTCCAATCCCAAAGGGGCCCAGATG
  CAGCCGATCTCCCTCCCCAGAGTTCAGCAGGTACCCCAGCAGGTGCAGCCGGTGCAGCACGTGTATCCT
  GCCCAGGTGCAGTACGTGGAAGGGGGAGACGCCGTCTACACCAATGGAGCCATACGAACAGCCTACACC
  TACAACCCCGAGCCTCAGATGTACGCCCCCAGCAGCACGGCTTCTTACTTCGAGGCCCCAGGCGGTGCC
  CAGGTGACCGTGGCAGCCTCGTCCCCGCCAGCGGTCCCCTCCCACAGCATGGTGGGCATCACCATGGAT
  GTCGGGGGGAGCCCCATCGTCTCCAGCGCGGGAGCCTATCTCATCCACGGGGGGATGGACAGCACCAGA
  CACTCCCTGGCCCACACCTCCCGCTCATCGCCCGCCACGCTCCAGTGGCTGTTGGATAATTATGAAACA
  GCGGAAGGTGTGAGTCTCCCCAGAAGTTCTCTTTACAACCACTACCTTCGGCACTGCCAGGAGCACAAG
  CTAGACCCAGTGAACGCCGCCTCCTTCGGGAAACTGATCCGTTCTGTGTTTATGGGGCTGAGAACGCGG
  CGGCTGGGCACCAGGGGCAACTCGAAGTACCATTACTATGGGATTCGTCTGAAGCCGGACTCACCACTG
  AACCGGCTGCAGGAGGACACGCAGTACATGGCCATGCGGCAGCAGCCCATGCACCAGAAGCCCAGGTAC
  CGGCCAGCCCAGAAGACGGACAGCCTCGGGGACAGCGGCTCCCACAGCGGCCTGCACAGCACTCCGGAA
  CAGACCATGGCCGTGCAGAGCCAGCACCACCAGCAGTACATAGATGTCTCCCACGTCTTCCCCGAGTTC
  CCAGCGCCCGACCTGGGCAGCTTCCTGCTGCAGGACGGCGTCACACTGCACGACGTCAAGGCCCTGCAG
  CTGGTGTACAGACGGCACTGCGAGGCAACTGTAGATGTGGTGATGAACCTCCAGTTCCACTACATCGAG
  AAGCTGTGGCTCTCCTTCTGGAACTCTAAGGCCTCCTCCAGCGACGGCCCCACCTCTCTTCCTGCCAGT
  GACGAAGACCCCGAGGGCGCCGTCCTGCCCAAGGACAAGCTTATCTCCCTGTGTCAGTGCGACCCCATC
  CTCAGGTGGATGAGGAGCTGCGACCACATCCTCTACCAGGCGCTGGTGGAGATTCTCATCCCCGACGTG
  CTGAGGCCGGTCCCCAGTACCTTGACACAGGCCATCCGTAACTTTGCCAAGAGCTTGGAAGGCTGGTTG
  ACAAATGCCATGAGTGACTTCCCACAACAGGTCATCCAGACCAAGGTGGGCGTCGTCAGTGCCTTCGCC
  CAGACGCTGCGGCGCTACACGTCCCTCAACCACCTGGCGCAGGCGGCCCGGGCGGTGCTGCAGAACACG
  TCCCAGATCAACCAGATGCTCAGCGACCTCAACCGCGTGGACTTTGCCAACGTGCAGGAGCAGGCCTCG
  TGGGTGTGCCAGTGCGAGGAGAGTGTGGTGCAGCGGCTGGAGCAGGATTTCAAGCTGACCCTGCAGCAG
  CAGAGCTCCCTGGACCAGTGGGCCAGCTGGCTGGACAGTGTGGTCACCCAGGTCCTGAAGCAGCATGCC
  GGCAGCCCCAGCTTCCCCAAGGCCGCCCGGCAGTTCTTGCTGAAATGGTCCTTTTACAGCTCCATGGTG
  ATCCGGGACCTGACCCTGCGCAGCGCTGCCAGCTTCGGCTCCTTCCACCTCATCCGCCTGCTCTACGAC
  GAGTACATGTTCTACCTGGTGGAGCACCGCGTCGCGGAGGCCACCGGAGAGACGCCGATCGCTGTGATG
  GGAGAGTTCAACGATCTCGCCTCTCTGTCGCTGACGCTGCTCGACAAAGATGACATGGGCGATGAGCAG
  CGTGGCAGCGAGGCGGGCCCAGACGCCCGCAGCCTGGGTGAGCCCCTGGTAAAGCGGGAGCGCAGTGAC
  CCCAACCACTCCCTGCAGGGCATCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_134433
• Insert Size: 2097 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134433.2
• RefSeq Size: 4039 bp
• RefSeq ORF: 2097 bp
• Locus ID: 5990
• UniProt ID: P48378
• Cytogenetics: 19p13.3
• Domains: RFX_DNA_binding, RFX1_trans_act
• Protein Families: Druggable Genome, Transcription Factors
• MW: 77.3 kDa
• Gene Summary: This gene is a member of the regulatory factor X gene family, which encodes transcription factors that contain a highly-conserved winged helix DNA binding domain. The protein encoded by this gene is structurally related to regulatory factors X1, X3, X4, and X5. It is a transcriptional activator that can bind DNA as a monomer or as a heterodimer with other RFX family members. This protein can bind to cis elements in the promoter of the IL-5 receptor alpha gene. Two transcript variants encoding different isoforms have been described for this gene, and both variants utilize alternative polyadenylation sites. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) includes an alternate in-frame exon, compared to variant 1, resulting in a shorter protein (isoform b) when it is compared to isoform a.