PDE7A (NM_002604) Human Untagged Clone

CAT#: SC313399

PDE7A (untagged)-Human phosphodiesterase 7A (PDE7A), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDE7A
• Synonyms: HCP1; PDE7
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_002604 edited
  TGGTACCTCAATGGAAGTGTGTTACCAGCTGCCGGTACTGCCCCTGGACAGGCCGGTCCC
  CCAGCACGTCCTCAGCCGCCGAGGAGCCATCAGCTTCAGCTCCAGCTCCGCTCTCTTCGG
  CTGCCCCAATCCCCGGCAGCTCTCTCAGAGGCGTGGAGCTATTTCCTATGACAGTTCTGA
  TCAGACTGCATTATACATTCGTATGCTAGGAGATGTACGTGTAAGGAGCCGAGCAGGATT
  TGAATCAGAAAGAAGAGGTTCTCACCCATATATTGATTTTCGTATTTTCCACTCTCAATC
  TGAAATTGAAGTGTCTGTCTCTGCAAGGAATATCAGAAGGCTACTAAGTTTCCAGCGATA
  TCTTAGATCTTCACGCTTTTTTCGTGGTACTGCGGTTTCAAATTCCCTAAACATTTTAGA
  TGATGATTATAATGGACAAGCCAAGTGTATGCTGGAAAAAGTTGGAAATTGGAATTTTGA
  TATCTTTCTATTTGATAGACTAACAAATGGAAATAGTCTAGTAAGCTTAACCTTTCATTT
  ATTTAGTCTTCATGGATTAATTGAGTACTTCCATTTAGATATGATGAAACTTCGTAGATT
  TTTAGTTATGATTCAAGAAGATTACCACAGTCAAAATCCTTACCATAACGCAGTCCACGC
  TGCGGATGTTACTCAGGCCATGCACTGTTACTTAAAGGAACCTAAGCTTGCCAATTCTGT
  AACTCCTTGGGATATCTTGCTGAGCTTAATTGCAGCTGCCACTCATGATCTGGATCATCC
  AGGTGTTAATCAACCTTTCCTTATTAAAACTAACCATTACTTGGCAACTTTATACAAGAA
  TACCTCAGTACTGGAAAATCACCACTGGAGATCTGCAGTGGGCTTATTGAGAGAATCAGG
  CTTATTCTCACATCTGCCATTAGAAAGCAGGCAACAAATGGAGACACAGATAGGTGCTCT
  GATACTAGCCACAGACATCAGTCGCCAGAATGAGTATCTGTCTTTGTTTAGGTCCCATTT
  GGATAGAGGTGATTTATGCCTAGAAGACACCAGACACAGACATTTGGTTTTACAGATGGC
  TTTGAAATGTGCTGATATTTGTAACCCATGTCGGACGTGGGAATTAAGCAAGCAGTGGAG
  TGAAAAAGTAACGGAGGAATTCTTCCATCAAGGAGATATAGAAAAAAAATATCATTTGGG
  TGTGAGTCCACTTTGCGATCGTCACACTGAATCTATTGCCAACATCCAGATTGGTAACTA
  TACATATTTAGATATAGCTGGTTAG
  
• Restriction Sites: Please inquire
• ACCN: NM_002604
• Insert Size: 1300 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_002604.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_002604.1, NP_002595.1
• RefSeq Size: 2990 bp
• RefSeq ORF: 1275 bp
• Locus ID: 5150
• Cytogenetics: 8q13.1
• Domains: PDEase, HDc
• Protein Families: Druggable Genome
• Protein Pathways: Progesterone-mediated oocyte maturation, Purine metabolism
• Gene Summary: The protein encoded by this gene belongs to the cyclic nucleotide phosphodiesterase (PDE) family, and PDE7 subfamily. This PDE hydrolyzes the second messenger, cAMP, which is a regulator and mediator of a number of cellular responses to extracellular signals. Thus, by regulating the cellular concentration of cAMP, this protein plays a key role in many important physiological processes. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]
  Transcript Variant: This variant (2, also called PDE7A3) differs in the 5' and 3' UTRs and in the 5' and 3' coding sequences compared to variant 1. The resulting isoform (b) has distinct N- and C-termini compared to isoform a.