TTYH1 (NM_020659) Human Untagged Clone

CAT#: SC310371

TTYH1 (untagged)-Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TTYH1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310371 representing NM_020659.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGGGCGCCCCCGGGCTACCGGCCCTCAGCTTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGAC
  TTCCAGCTCCGCCCGGTGCCCAGCGTTTTCGCGCCCCAAGAGCAGGAATACCAGCAGGCCTTGTTGCTG
  GTGGCGGCCTTGGCGGGCCTGGGCTTGGGCCTGAGCCTCATTTTCATCGCTGTCTACCTCATCCGCTTC
  TGCTGCTGCCGGCCCCCCGAGCCCCCCGGGTCCAAGATCCCCTCGCCCGGGGGAGGCTGCGTCACCTGG
  AGCTGCATTGTCGCCCTTCTCGCCGGCTGCACTGGCATTGGCATCGGTTTCTATGGCAACAGTGAGACC
  AGTGATGGGGTGTCCCAGCTCAGCTCTGCGCTGCTGCACGCCAACCACACACTCAGCACCATTGACCAC
  CTGGTGTTGGAGACGGTGGAGAGGCTGGGCGAGGCGGTGAGGACAGAGCTGACCACCCTGGAGGAGGTG
  CTCGAGCCGCGCACGGAGCTGGTGGCTGCCGCCCGAGGGGCTCGACGGCAGGCGGAGGCTGCGGCCCAG
  CAGCTGCAGGGGCTGGCCTTCTGGCAGGGAGTGCCCCTGAGCCCCCTGCAGGTGGCTGAAAATGTGTCC
  TTTGTGGAGGAGTACAGGTGGCTGGCCTACGTCCTCCTGCTGCTCCTGGAGCTGCTGGTCTGCCTCTTC
  ACCCTCCTGGGCCTGGCGAAGCAGAGCAAGTGGCTGGTGATCGTGATGACAGTCATGAGTCTCCTGGTT
  CTCGTCCTGAGCTGGGGCTCCATGGGCCTGGAGGCAGCCACGGCCGTGGGCCTCAGTGACTTCTGCTCC
  AATCCAGACCCTTATGTTCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTAT
  TATCTCCTCTGCAACCGGGCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCTG
  GCCAACATCCACTCCCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTCAGCGCAGAAG
  CCTCTGCTGTCCTTGGAGGAGACTCTGAATGTGACAGAAGGAAATTTCCACCAGTTGGTGGCACTGCTA
  CACTGCCGCAGCCTGCACAAGGACTATGGTGCAGCCCTGCGGGGCCTGTGCGAAGACGCCCTGGAAGGC
  CTGCTCTTCCTGCTACTCTTCTCCCTGCTGTCTGCAGGAGCGCTGGCCACTGCCCTCTGCAGCCTGCCC
  CGAGCCTGGGCCCTCTTCCCACCCAGTGACGACTACGATGACACAGACGATGACGACCCTTTCAACCCT
  CAGGAATCCAAGCGCTTTGTGCAGTGGCAGTCGTCTATCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_020659
• Insert Size: 1353 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_020659.3
• RefSeq Size: 1921 bp
• RefSeq ORF: 1353 bp
• Locus ID: 57348
• UniProt ID: Q9H313
• Cytogenetics: 19q13.42
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 49.1 kDa
• Gene Summary: This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent, volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jan 2011]
  Transcript Variant: This variant (1) contains an alternate exon compared to variant 2, that causes a frameshift. The resulting isoform (1) has a shorter and distinct C-terminus compared to isoform 2.