FLAD1 (NM_025207) Human Untagged Clone

CAT#: SC310239

FLAD1 (untagged)-Human FAD1 flavin adenine dinucleotide synthetase homolog (S. cerevisiae) (FLAD1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FLAD1
• Synonyms: FAD1; FADS; LSMFLAD; PP591
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310239 representing NM_025207.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGTTGGGATTTGGGAACACGTTTATTCCAGAGGCAGGAACAAAGGAGTCGCTTGTCAAGGATCTGG
  TTAGAGAAGACTAGGGTCTTCCTCGAAGGAAGCACGCGCACGCCTGCTCTCCCCCATTGTCTTTTCTGG
  CTTCTCCAGGTTCCCTCGACCCAGGACCCCCTGTTCCCAGGCTATGGCCCCCAGTGCCCTGTAGACCTG
  GCAGGCCCCCCGTGCTTGCGACCCCTATTTGGGGGTCTGGGTGGCTACTGGAGGGCCTTGCAGAGGGGC
  AGAGAAGGCAGGACCATGACATCTAGGGCCTCTGAACTTTCTCCGGGGCGCAGCGTGACGGCTGGCATC
  ATCATTGTTGGAGATGAGATCCTTAAGGGACACACTCAGGACACCAACACCTTCTTTCTGTGCCGGACA
  CTGCGCTCCCTAGGGGTCCAGGTTTGCCGAGTCTCAGTTGTACCTGATGAGGTAGCCACCATTGCAGCT
  GAGGTCACTTCTTTCTCCAACCGCTTCACCCATGTCCTCACAGCAGGGGGCATCGGCCCCACTCATGAT
  GATGTGACCTTTGAGGCAGTGGCACAGGCCTTTGGAGATGAGCTGAAGCCACACCCCAAGTTGGAAGCA
  GCCACCAAAGCCCTAGGAGGGGAAGGCTGGGAGAAGCTATCATTGGTGCCCTCCTCTGCCCGCCTGCAT
  TATGGCACAGATCCTTGCACTGGTCAACCTTTCAGATTCCCTCTGGTCTCCGTCCGAAACGTCTACCTC
  TTCCCAGGCATTCCAGAGCTGCTGCGGCGGGTGCTGGAGGGGATGAAGGGACTATTCCAAAACCCAGCT
  GTTCAGTTCCACTCAAAGGAGCTATATGTGGCTGCTGATGAAGCCTCCATCGCCCCCATTCTGGCTGAG
  GCCCAGGCCCACTTTGGACGTAGGCTTGGCCTGGGTTCCTACCCTGACTGGGGCAGCAACTACTATCAG
  GTGAAGCTGACTCTAGACTCAGAGGAAGAAGGACCCCTGGAGGAATGCTTGGCCTACCTGACTGCCCGT
  TTGCCCCAGGGATCGCTGGTCCCCTACATGCCCAACGCTGTGGAGCAGGCCAGTGAGGCTGTATACAAA
  CTCGCTGAATCAGGGTCTTCTTTGGGGAAAAAGGTGGCAGGTGCCCTACAGACCATTGAGACCTCCCTG
  GCTCAGTACAGCCTCACCCAGCTCTGTGTGGGCTTCAACGGGGGCAAAGACTGCACTGCCCTCCTGCAC
  CTCTTCCATGCAGCTGTGCAGAGGAAATTACCTGATGTTCCAAACCCCCTCCAGATCCTGTATATCCGC
  AGCATCTCCCCTTTCCCTGAGCTGGAACAGTTTCTACAGGACACTATCAAGAGGTATAATCTGCAGATG
  TTGGAAGCTGAGGGCAGCATGAAGCAGGCCCTGGGTGAACTGCAGGCACGGCACCCCCAGCTGGAGGCT
  GTCCTTATGGGCACCCGCCGGACTGACCCCTACTCCTGTAGCCTCTGCCCTTTCAGCCCCACTGACCCA
  GGCTGGCCCGCATTCATGCGCATCAACCCACTGCTGGACTGGACCTACAGAGACATCTGGGATTTTCTG
  CGTCAGCTGTTTGTCCCATACTGTATCCTGTATGACCGAGGATACACATCACTGGGGAGTCGGGAGAAT
  ACCGTGCGGAACCCGGCCCTGAAGTGCCTGAGCCCAGGAGGACACCCCACATACCGTCCAGCCTATCTA
  CTGGAGAACGAAGAAGAGGAGCGGAACTCCCGCACATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_025207
• Insert Size: 1764 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_025207.4
• RefSeq Size: 2260 bp
• RefSeq ORF: 1764 bp
• Locus ID: 80308
• UniProt ID: Q8NFF5
• Cytogenetics: 1q21.3
• Domains: MoCF_biosynth
• Protein Pathways: Metabolic pathways, Riboflavin metabolism
• MW: 65.3 kDa
• Gene Summary: This gene encodes the enzyme that catalyzes adenylation of flavin mononucleotide (FMN) to form flavin adenine dinucleotide (FAD) coenzyme. Alternatively spliced transcript variants encoding distinct isoforms have been observed. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest protein (isoform 1).