GAPex 5 (GAPVD1) (NM_015635) Human Untagged Clone

CAT#: SC309909

GAPVD1 (untagged)-Human GTPase activating protein and VPS9 domains 1 (GAPVD1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GAPVD1
• Synonyms: GAPex-5; GAPEX5; RAP6
• Vector: pCMV6 series
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_015635, the custom clone sequence may differ by one or more nucleotides
  ATGGTGAAACTAGATATTCATACTCTGGCTCATCACCTCAAGCAGGAACGCTTATATGTA
  AACTCTGAGAAACAGCTCATTCAGAGGCTCAATGCAGATGTACTTAAGACAGCTGAAAAG
  TTGTATCGTACAGCATGGATTGCGAAGCAACAGAGAATCAATTTGGATCGGCTTATCATA
  ACCAGTGCTGAAGCTTCCCCTGCTGAATGTTGCCAACATGCCAAAATTTTGGAAGATACA
  CAATTTGTTGATGGGTATAAGCAATTGGGATTTCAGGAGACTGCTTATGGAGAATTCTTG
  AGTCGATTGAGGGAAAATCCTCGTCTTATTGCCTCCTCTTTGGTTGCTGGAGAGAAACTT
  AATCAGGAGAACACACAAAGTGTTATTTACACAGTTTTTACCTCCCTGTATGGCAATTGC
  ATCATGCAAGAAGATGAAAGCTACCTCCTTCAGGTTTTGCGATACTTGATTGAATTTGAA
  CTTAAAGAAAGTGACAACCCTAGGCGACTTTTGAGGAGAGGAACTTGTGCCTTCAGCATC
  TTATTTAAACTTTTTTCTGAAGGACTGTTTTCTGCCAAACTTTTCCTCACAGCCACTTTA
  CATGAGCCAATTATGCAACTGCTTGTTGAAGATGAAGATCACCTGGAAACAGATCCAAAC
  AAGCTAATTGAGAGGTTCTCTCCATCTCAGCAGGAAAAACTCTTTGGAGAGAAGGGCTCA
  GATAGATTCAGGCAAAAAGTTCAAGAAATGGTGGAGTCCAATGAAGCAAAGCTAGTGGCT
  TTGGTGAACAAATTTATTGGTTATCTCAAACAGAACACATATTGTTTTCCACATAGTTTA
  AGGTGGATCGTGTCTCAGATGTACAAAACCCTCTCCTGTGTAGATAGGCTGGAAGTTGGG
  GAGGTCAGGGCAATGTGTACTGATCTCCTGTTGGCCTGCTTCATTTGTCCTGCAGTTGTC
  AATCCAGAACAATATGGAATAATTTCCGATGCTCCTATTAATGAAGTAGCACGATTTAAT
  CTGATGCAGGTAGGCCGCCTTTTGCAGCAGTTAGCAATGACTGGCTCTGAAGAGGGAGAT
  CCCCGAACAAAGAGCAGCCTTGGAAAGTTTGACAAAAGCTGTGTTGCCGCTTTCCTTGAT
  GTTGTGATTGGGGGCCGTGCAGTGGAGACCCCTCCATTGTCTTCCGTCAATCTTCTGGAA
  GGATTGAGCAGAACTGTGGTTTATATAACCTACAGTCAGCTTATTACTCTGGTGAATTTT
  ATGAAGAGTGTGATGTCTGGAGATCAACTGAGAGAAGATAGAATGGCTCTTGACAATTTA
  TTGGCAAACCTACCCCCGGCCAAGCCAGGAAAAAGTAGCAGTTTAGAAATGACTCCCTAC
  AATACACCTCAGCTATCTCCAGCAACCACTCCAGCAAATAAAAAGAATCGATTACCTATA
  GCAACTCGGAGCAGAAGCCGCACCAATATGCTAATGGACCTACATATGGACCATGAAGGA
  TCATCTCAAGAAACCATCCAGGAGGTGCAACCAGAAGAGGTGTTGGTCATTTCCTTAGGT
  ACAGGTCCCCAGCTTACTCCAGGGATGATGTCAGAAAATGAGGTCCTAAACATGCAGCTT
  TCGGATGGAGGACAAGGAGATGTCCCTGTTGATGAAAACAAACTCCATGGTAAACCTGAT
  AAAACCTTGCGCTTTTCCCTCTGCAGTGATAATCTGGAAGGAATATCTGAAGGTCCTTCA
  AATCGCTCCAATTCAGTGTCCTCCCTAGACCTAGAAGGAGAGTCTGTGTCAGAACTTGGA
  GCAGGACCTTCTGGCAGTAATGGAGTTGAAGCTCTACAGCTGTTAGAACATGAGCAAGCT
  ACAACACAGGATAACCTTGATGATAAGCTAAGGAAGTTTGAAATTCGTGACATGATGGGA
  TTAACAGATGATAGGGACATATCAGAAACAGTGAGTGAGACCTGGAGTACAGACGTCTTG
  GGAAGTGACTTTGACCCTAATATTGATGAAGATCGCTTGCAAGAAATTGCAGGTGCTGCA
  GCAGAGAACATGTTAGGCAGTTTGCTGTGCCTCCCAGGTTCAGGGTCAGTGCTTCTTGAC
  CCCTGCACTGGTTCTACCATATCAGAGACAACAAGTGAAGCTTGGAGTGTAGAGGTATTG
  CCAAGTGACTCAGAGGCCCCAGACCTAAAGCAGGAGGAGCGTCTGCAAGAACTGGAGAGC
  TGTTCTGGACTGGGTAGCACATCTGATGATACGGATGTCAGGGAGGTCAGTTCCCGCCCC
  AGCACACCAGGCCTCAGTGTTGTGTCCGGCATAAGTGCAACCTCTGAGGATATTCCCAAT
  AAGATTGAAGACCTGAGATCTGAGTGCAGCTCTGATTTTGGGGGTAAAGATTCTGTCACT
  AGTCCAGACATGGATGAAATAACTCACGATTTTCTTTATATACTTCAGCCAAAACAACAT
  TTTCAACACATTGAAGCAGAAGCAGACATGAGAATCCAGCTGTCTTCTAGTGCCCACCAG
  CTGACCTCTCCTCCTTCTCAGTCAGAGTCTCTGCTGGCCATGTTTGATCCACTGTCTTCA
  CATGAAGGGGCTTCTGCTGTGGTAAGGCCAAAGGTTCACTATGCTAGGCCATCGCATCCA
  CCACCAGATCCCCCAATCCTGGAAGGAGCTGTGGGAGGAAATGAGGCCAGGTTGCCAAAC
  TTTGGTTCCCATGTTTTAACTCCAGCTGAAATGGAGGCATTCAAGCAAAGGCATTCTTAC
  CCTGAGAGACTAGTTCGAAGCAGGAGCTCTGATATAGTATCTTCTGTCCGGAGACCCATG
  AGTGACCCCAGCTGGAACCGGCGTCCAGGAAATGAAGAGCGAGAACTCCCTCCAGCTGCA
  GCCATTGGTGCTACTTCTTTGGTGGCTGCACCTCATTCATCATCTTCATCCCCGAGTAAG
  GACTCCTCAAGAGGAGAGACTGAAGAACGCAAAGATAGCGATGATGAGAAATCAGACAGG
  AACAGACCTTGGTGGAGAAAACGTTTTGTTTCAGCCATGCCTAAAGCTCCTATACCATTT
  AGAAAGAAAGAAAAACAAGAAAAAGACAAAGATGATCTGGGGCCTGACAGATTCTCAACA
  CTCACAGATGATCCCAGCCCTAGACTCAGTGCACAAGCTCAGGTGGCTGAGGATATTCTG
  GACAAATACAGGAATGCCATTAAACGGACCAGCCCCAGTGATGGAGCAATGGCAAACTAT
  GAAAGTACAGAGGTTATGGGTGATGGTGAAAGTGCACATGATTCTCCCCGTGACGAAGCA
  CTGCAGAACATCTCGGCTGATGATCTCCCAGACTCTGCAAGCCAAGCAGCCCACCCGCAG
  GATTCAGCTTTCTCTTACAGAGATGCAAAAAAGAAACTGAGGCTTGCTCTTTGCTCTGCG
  GACTCTGTTGCCTTCCCAGTGCTGACCCATTCAACAAGGAATGGTTTACCAGACCACACA
  GACCCAGAAGACAATGAAATTGTATGCTTCTTAAAAGTTCAAATAGCTGAAGCAATTAAT
  TTACAAGATAAGAATCTAATGGCTCAACTTCAAGAAACAATGCGCTGTGTGTGCCGTTTT
  GATAATAGGACTTGTAGGAAACTGCTGGCTTCGATTGCTGAGGACTACAGAAAAAGAGCC
  CCATATATTGCTTATCTCACTCGTTGTCGACAAGGACTACAGACCACACAGGCTCACCTG
  GAAAGGCTATTGCAAAGAGTTTTGCGGGACAAAGAAGTGGCCAATCGATACTTTACCACT
  GTCTGTGTGAGATTACTGCTTGAGAGCAAAGAAAAGAAGATCAGGGAATTCATTCAAGAC
  TTTCAGAAACTCACCGCAGCTGACGATAAAACTGCTCAGGTAGAAGATTTTCTGCAGTTT
  CTTTATGGTGCAATGGCCCAGGATGTCATATGGCAAAACGCGAGTGAAGAACAGCTTCAA
  GATGCACAGCTGGCCATTGAGCGAAGCGTGATGAACCGGATTTTCAAGCTCGCCTTCTAC
  CCTAATCAAGATGGGGACATACTTCGCGACCAGGTTCTTCATGAACATATCCAGAGATTG
  TCTAAAGTAGTGACTGCAAATCACAGAGCTCTTCAGATACCAGAGGTTTATCTTCGAGAA
  GCACCATGGCCATCTGCACAATCAGAAATCAGGACAATAAGTGCTTATAAAACCCCCCGG
  GACAAAGTGCAGTGCATCCTGAGAATGTGCTCTACGATTATGAACCTCCTGAGCCTGGCC
  AATGAGGACTCTGTCCCTGGAGCGGATGACTTTGTTCCTGTGTTGGTGTTTGTGTTGATA
  AAGGCAAATCCACCCTGTTTGCTGTCTACTGTGCAGTATATCAGTAGCTTTTATGCTAGC
  TGTCTGTCTGGAGAGGAGTCCTATTGGTGGATGCAGTTCACAGCAGCAGTAGAATTCATT
  AAAACCATCGATGACCGAAAGTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_015635
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015635.2, NP_056450.2
• RefSeq Size: 6906 bp
• RefSeq ORF: 4464 bp
• Locus ID: 26130
• UniProt ID: Q14C86
• Cytogenetics: 9q33.3
• Gene Summary: Acts both as a GTPase-activating protein (GAP) and a guanine nucleotide exchange factor (GEF), and participates in various processes such as endocytosis, insulin receptor internalization or LC2A4/GLUT4 trafficking. Acts as a GEF for the Ras-related protein RAB31 by exchanging bound GDP for free GTP, leading to regulate LC2A4/GLUT4 trafficking. In the absence of insulin, it maintains RAB31 in an active state and promotes a futile cycle between LC2A4/GLUT4 storage vesicles and early endosomes, retaining LC2A4/GLUT4 inside the cells. Upon insulin stimulation, it is translocated to the plasma membrane, releasing LC2A4/GLUT4 from intracellular storage vesicles. Also involved in EGFR trafficking and degradation, possibly by promoting EGFR ubiquitination and subsequent degradation by the proteasome. Has GEF activity for Rab5 and GAP activity for Ras.[UniProtKB/Swiss-Prot Function]