AKAP11 (NM_016248) Human Untagged Clone

CAT#: SC309762

AKAP11 (untagged)-Human A kinase (PRKA) anchor protein 11 (AKAP11)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: AKAP11
• Synonyms: AKAP-11; AKAP220; PPP1R44; PRKA11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC309762 representing NM_016248.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGACTTTCAGAAACAATCACATGAAGACTAAAGCATCTGTCAGAAAAAGCTTCAGTGAAGATGTG
  TTCCAGTCTGTAAAGTCTTTATTGCAGAGTCAGAAGGAACTATGCAGTGTAACAGCAGAGGACTGTTTA
  CAGCAGGATGAGCATGCCAATTTAACTGAGGTCACATTTCTGGGTTTTAATGAAGAGACAGATGCTGCT
  CATATACAGGATTTAGCTGCAGTTTCTTTGGAACTTCCAGATATTCTGAATTCACTCCACTTCTGCAGT
  CTAAATGAAAATGAAATTATTTGTATGAAGAATATAAATAAACCATTAGATATAAGCAGTGATCCTCTA
  AATCAGAGTCATCCTTCTGGAATGCTTTGTGTCATGAGAGTGTCACCTACATCACCAAGACTTAGGATT
  GATTTTATCTTTAGTCTCCTAAGTAAATATGCTACTGGTATAAGGTACACCTTGGACACATTCTTGCAT
  CAGAAGCACCAACTTGAGACCACTGATGAAGATGATGATGATACTAACCAGTCTGTGTCATCCATAGAG
  GATGACTTTGTCACTGCTTTTGAGCACTTAGAAGAGGAAGAGACTTCAAAGCCATACAATGATGGAATG
  AACATTACTGTGCTAAGGAGCCAGTGTGATGCTGCTTCCCAGACGGTTACTGGTCATCATTTAGAAACC
  CATGATTTAAAGATTCTCATTAGCTCTGGACAGCAGAAGTCATTGGCTAAACCCTCAACTTCCTCAGTG
  AATGTCCTGGGACATAAAGAACTACCTTCTGTGAAAACTTCAGTCACAACATCAATTTCAGAGCCTTGG
  ACCCAAAGGAGTTTCTATAGGTCATCTAATGCTTCAGATAAAGATAGTGATTTACAGAAAACATTTTTT
  TCGTCTTCTCCTGCCTACTCATCTGAATCAGAATGTTCAAGTCCAAGTCCTGTTATTTTCTTGGATGAA
  GAGGGATATCAAAAAAGCTTAAAAGCAAAACTTGAGCTGCCTAAAATTCCTGTGATGAAAGATGATATA
  GAGGATTCAGACTCAGAAGTAAGTGAATTTTTTGATAGTTTTGATCAGTTTGATGAACTAGAACAAACT
  TTAGAGACTTGCCTGTTTAACAAAGATCCCGTCATAGGGAAGTCATCGCAGAGGAAAGGGCACAAACAT
  GGAAAGTCATGTATGAATCCTCAAAAATTCAAGTTTGATCGTCCAGCTCTCCCAGCTAATGTTAGAAAG
  CCAACTCCTCGTAAACCAGAATCTCCATATGGTAACCTGTGTGATGCTCCGGATTCTCCTCGCCCAGTG
  AAGGCATCAAGGGAAGATAGTGGTTTATTTAGTCCTATTCGATCCTCTGCTTTTAGTCCTCTTGGAGGC
  TGTACTCCAGCTGAATGTTTTTGCCAAACAGATATTGGTGGAGATAGGATTCATGAAAATCATGATTCT
  GTTTATTACACCTATGAAGACTATGCAAAAAGCATTTCATGTGAAGTACTAGGCTCAGTTCTTCGTACC
  CACCATACTAATACCCTATCAAATATTAACAGTATTAAACATGGAGAAAATAAAACTGTAACTTTTAAG
  CATGGAAACCTTGATCAAAAAAATAAATCTAAAAATAAATCCTTAATGATTAAAGATAGCATTCAAAAA
  TTTGCAGCAGATCTTGTGGAAAAAAGTTTTGGCAGTGCATTTAAAGACTTACAGAAAGGAGTCTCTTCA
  TGTACCAATGCTTTGTACCACTTAGCCATCAAATTGACATCATCTGTTTTGCAGATGGCATTTGATGAG
  CTGAGAAGGCAGCGTGCATTTTCACTAAAAGAACGTGCCATTAGTGGCCTGGCTAACTTTTTGGTGAGT
  GAAGCTTTATCAAATGCCTTAAAAGATTTACAGTATGTAAAGAAGCAGATATTCACAAACACAGTTGCT
  AGGTTTGCTGCAGATCTTGCTGAAGAGCTTGTTTTTGAAGGCATCATGGAGGTGTGTCAGTTTTCATAT
  CCTCAAACGCCTGCATCTCCACAGTGTGGGTCGTTTGACTTTGAAGACAAAGTAGTGAAGTTGTATGCA
  AAAGATTTGTCTGAATCTGTAATACAGGAAGCATTCATTGAGCTATCACAAGTTGATGTGACCTTTACA
  ACAAAGGCAGCAGTTAGTGTCTCTACGGATAATATCAAGTATGTGAGTGCAGAAAGTGTAGTGCCATCG
  ACACAGGCTGTCACGTTTTCCCCTTCTTTTCACAATCAAGCAATTATGGTGACAAAACCAGTGCAGGAA
  TATAAAAAGGAATACACAGTGCAGCAGGCCTTGTTTTGTACTTCTGGAATTGTTACTTCTATACCGGTG
  CCCTTGGCAGGAAGTGCCCTTCTCCCATATCATATTTCATCTACTGCATGTCAGGCCAAGGCTCATCTG
  TCATCTGATGATAGTAATTCAAATGGTGATTCTGCCCAAGTGCATATTGCCACAAAAAACAGAGAAGAA
  AAAGCAGCTTGTCTCAGAAATATTTGTTTACCTTCAGAACACAATCCAGGTAATCAGAATGATTTTAAA
  CCAACTAATGACGATATTGAAATGCAGAGTTCCTCAAAATTACCAAATGATCCTGCAATTATTAGCAAC
  TTTTCTGCAGCAGTGGTGCATACGATAGTAAATGAAACTTTAGAGTCAATGACATCATTGGAAGTTACA
  AAAATGGTTGATGAACGTACAGATTATTTAACTAAATCTTTAAAGGAGAAAACCCCTCCATTTTCCCAC
  TGTGATCAGGCAGTGCTGCAATGCAGTGAAGCTAGTAGCAATAAGGACATGTTTGCTGACCGGTTATCT
  AAATCTATTATTAAACATTCCATAGATAAGAGCAAATCAGTGATCCCAAATATAGATAAAAATGCAGTA
  TACAAGGAAAGCTTGCCTGTTTCTGGAGAAGAATCACAGTTGACACCAGAAAAGTCTCCCAAATTTCCT
  GACTCTCAGAATCAGTTAACTCACTGCTCACTTTCAGCTGCAAAGGATTGTGTTCCAGAATGTAAAGTT
  TCTATGGTTCATGGATCCTCCCTAGAGACACTGCCATCTTGTCCAGCTGTGACAGGTCAGAAATCTGAC
  TTGAAGGAATCTGCTAAGGATCAACCACTGAAAAAGCATAACTTGAATAGTACATCACTTGAGGCCTTG
  TCTTTTGGACAGGAAAACCCCTTTCCTCATTCACATACTTTCTCATCTACAGCACTTACCTGTGTAGAT
  GGTTTGCATGTGGAAGATAAACAGAAAGTCAGAGACAGAAATGTAATACCTGATACTCCTCCATCAACT
  CCTCTAGTACCATCCCGGGCTAGTTCTGAATGGGATATCAAGAAGTTAACTAAAAAGCTCAAGGGAGAA
  TTAGCCAAAGAGTTTGCACCTGCTACACCACCTTCTACTCCACACAACTCATCTGTTGGTAGTTTGTCT
  GAGAATGAACAAAATACTATAGAAAAAGAAGAGTTCATGTTGAAACTCATGCGATCTCTTTCTGAAGAA
  GTTGAGAGTAGTGAAAGTGGAGAGCTCCCAGAAGTGGATGTGAAGTCGGAGCACTCAGGGAAGAAGGTT
  CAGTTTGCAGAAGCATTAGCTACACACATCCTTTCTCTTGCAACTGAAATGGCAGCTTCCCATTTAGAT
  AACAAAATAATTCAAGAACCCAAGGTTAAAAACCCTTGCTTAAATGTGCAAAGTCAAAGAAGTGTGTCG
  CCTACTTTTTTAAACCCCTCAGACGAAAATTTGAAAACATTATGCAATTTTGCGGGTGATCTGGCAGCA
  GAAGTCATTACAGAAGCTGAGAAAATAGCAAAAGTCCGAAATTGTATGCTTTTCAAGCAAAAGAAGAAC
  AGTTGTTATGCTGATGGTGACGAAGATTATAAAGTAGAAGAGAAGTTGGATATAGAGGCTGTAGTGCAC
  CCAAGAGAAGTGGATCCGTTTATTCTTTCATTACCACCAAGTTCTTGTATGTCAGGTCTGATGTATAAG
  TATCCCAGCTGTGAAAGTGTGACAGATGAATATGCAGGTCACCTTATTCAGATACTAAAACAGGAAGGT
  GGTAATAGTGAGTTGATAATGGATCAGTATGCCAATAGGCTTGCCTACCGATCTGTTAAATCAGGATTA
  CAGGAAGCAGCTAAGACAACCAAAGTGCAGTGCAACTCAAGAATGTTCCCTGTGCCAAGTTCACAAGTG
  AAAACAAACAAGGAACTGTTAATGTTTTCAAACAAAGAGCACCACCAAGAAGCAGACAAAAAGAGACAA
  AGTAAAAGAAATGAAGGTTACTTTTGTAAAAATCAAACTTGTGAAAGGACCCTGGATCCATATAGAAAT
  GAGGTCTCCCAACTGTATAGTTTTTCAACCTCTCTGGTTCACAGCATAACAAAAGATGCTAAGGAAGAG
  TTGACAGCCTCTCTAGTTGGCCTACCAAAATCCTTAACAGATTCTTGCTTGTTTGAAAAATCTGGATAT
  GAAGAAGATAATGAGTGTCACGTTACACCAGAATTGCCTAAGTCTCTTCAGCCTTCCTCACAAAATCAC
  AGGTTTTACCACAGCACTGGCAGTTTAAATGGATATGGTTGTGGAGACAATGTTGTTCAAGCTGTAGAA
  CAGTATGCCAAAAAAGTAGTGGATGACACTCTAGAGCTAACTCTAGGATCTACAGTGTTCCGAGTGTCT
  GAGACCACAAAATCAGCAGACAGGGTCACTTATGCAGAAAAGTTGTCACCTCTTACAGGTCAAGCTTGC
  AGATACTGTGACCTTAAAGAACTCCACAATTGCACTGGAAATTCATCTCAGCACTTTTTCAGACAGGGT
  TCTCTCGCCAGTAGTAAGCCAGCTTCTAATCCAAAATTTAGCAGCCGCTATCAGAAATCTAGGATTTTT
  CATCTCAGTGTCCCTCAGATTCATGTTAATCTTGATAAGAAGGCAGTGCTTGCTGAGAAGATAGTTGCT
  GAAGCCATTGAAAAAGCTGAGCGAGAGCTGAGCAGTACCAGCCTGGCAGCCGACAGTGGGATCGGACAG
  GAGGGTGCCAGCTTTGCTGAAAGCCTTGCCACAGAAACCATGACAGCAGCTGTCACAAATGTTGGGCAT
  GCTGTTAGCAGTTCAAAAGAAATAGAAGACTTTCAGTCAACCGAGTCTGTCAGTAGCCAGCAGATGAAC
  CTCAGTATTGGTGATGACAGCACTGGTAGCTGGTCCAATTTAAGTTTTGAAGATGAACACCAAGATGAA
  AGCAGCAGTTTTCATCATCTAAGTGAAAGTAATGGTAACAGCAGTAGCTGGAGCAGTCTTGGTTTAGAA
  GGAGATTTGTATGAGGACAATTTATCCTTTCCAACATCAGACAGTGATGGACCAGATGATAAAGATGAA
  GAGCATGAGGACGAAGTAGAAGGTTTGGGGCAAGATGGAAAGACACTGCTAATTACGAATATTGACATG
  GAGCCATGCACGGTAGACCCCCAGCTAAGGATTATTCTTCAGTGGCTCATTGCCTCTGAGGCTGAAGTT
  GCAGAACTTTATTTTCATGACTCTGCAAATAAGGAGTTTATGCTACTTTCAAAACAATTACAAGAGAAA
  GGATGGAAAGTGGGAGACCTCCTGCAGGCTGTGCTTCAATACTATGAAGTGATGGAAAAAGCTTCCAGT
  GAGGAGAGATGCAAGTCGCTGTTTGATTGGCTCTTGGAAAATGCATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_016248
• Insert Size: 5706 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016248.3
• RefSeq Size: 9926 bp
• RefSeq ORF: 5706 bp
• Locus ID: 11215
• UniProt ID: Q9UKA4
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome
• MW: 210.5 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. [provided by RefSeq, Jul 2008]