AKAP11 (NM_016248) Human Tagged ORF Clone

CAT#: RC220532

AKAP11 (Myc-DDK-tagged)-Human A kinase (PRKA) anchor protein 11 (AKAP11)

Product Images

Circular map for RC220532

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: AKAP11
• Synonyms: AKAP-11; AKAP220; PPP1R44; PRKA11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220532 representing NM_016248
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACTTTCAGAAACAATCACATGAAGACTAAAGCATCTGTCAGAAAAAGCTTCAGTGAAGATGTGT
  TCCAGTCTGTAAAGTCTTTATTGCAGAGTCAGAAGGAACTATGCAGTGTAACAGCAGAGGACTGTTTACA
  GCAGGATGAGCATGCCAATTTAACTGAGGTCACATTTCTGGGTTTTAATGAAGAGACAGATGCTGCTCAT
  ATACAGGATTTAGCTGCAGTTTCTTTGGAACTTCCAGATATTCTGAATTCACTCCACTTCTGCAGTCTAA
  ATGAAAATGAAATTATTTGTATGAAGAATATAAATAAACCATTAGATATAAGCAGTGATCCTCTAAATCA
  GAGTCATCCTTCTGGAATGCTTTGTGTCATGAGAGTGTCACCTACATCACCAAGACTTAGGATTGATTTT
  ATCTTTAGTCTCCTAAGTAAATATGCTACTGGTATAAGGTACACCTTGGACACATTCTTGCATCAGAAGC
  ACCAACTTGAGACCACTGATGAAGATGATGATGATACTAACCAGTCTGTGTCATCCATAGAGGATGACTT
  TGTCACTGCTTTTGAGCACTTAGAAGAGGAAGAGACTTCAAAGCCATACAATGATGGAATGAACATTACT
  GTGCTAAGGAGCCAGTGTGATGCTGCTTCCCAGACGGTTACTGGTCATCATTTAGAAACCCATGATTTAA
  AGATTCTCATTAGCTCTGGACAGCAGAAGTCATTGGCTAAACCCTCAACTTCCTCAGTGAATGTCCTGGG
  ACATAAAGAACTACCTTCTGTGAAAACTTCAGTCACAACATCAATTTCAGAGCCTTGGACCCAAAGGAGT
  TTCTATAGGTCATCTAATGCTTCAGATAAAGATAGTGATTTACAGAAAACATTTTTTTCGTCTTCTCCTG
  CCTACTCATCTGAATCAGAATGTTCAAGTCCAAGTCCTGTTATTTTCTTGGATGAAGAGGGATATCAAAA
  AAGCTTAAAAGCAAAACTTGAGCTGCCTAAAATTCCTGTGATGAAAGATGATATAGAGGATTCAGACTCA
  GAAGTAAGTGAATTTTTTGATAGTTTTGATCAGTTTGATGAACTAGAACAAACTTTAGAGACTTGCCTGT
  TTAACAAAGATCCCGTCATAGGGAAGTCATCGCAGAGGAAAGGGCACAAACATGGAAAGTCATGTATGAA
  TCCTCAAAAATTCAAGTTTGATCGTCCAGCTCTCCCAGCTAATGTTAGAAAGCCAACTCCTCGTAAACCA
  GAATCTCCATATGGTAACCTGTGTGATGCTCCGGATTCTCCTCGCCCAGTGAAGGCATCAAGGGAAGATA
  GTGGTTTATTTAGTCCTATTCGATCCTCTGCTTTTAGTCCTCTTGGAGGCTGTACTCCAGCTGAATGTTT
  TTGCCAAACAGATATTGGTGGAGATAGGATTCATGAAAATCATGATTCTGTTTATTACACCTATGAAGAC
  TATGCAAAAAGCATTTCATGTGAAGTACTAGGCTCAGTTCTTCGTACCCACCATACTAATACCCTATCAA
  ATATTAACAGTATTAAACATGGAGAAAATAAAACTGTAACTTTTAAGCATGGAAACCTTGATCAAAAAAA
  TAAATCTAAAAATAAATCCTTAATGATTAAAGATAGCATTCAAAAATTTGCAGCAGATCTTGTGGAAAAA
  AGTTTTGGCAGTGCATTTAAAGACTTACAGAAAGGAGTCTCTTCATGTACCAATGCTTTGTACCACTTAG
  CCATCAAATTGACATCATCTGTTTTGCAGATGGCATTTGATGAGCTGAGAAGGCAGCGTGCATTTTCACT
  AAAAGAACGTGCCATTAGTGGCCTGGCTAACTTTTTGGTGAGTGAAGCTTTATCAAATGCCTTAAAAGAT
  TTACAGTATGTAAAGAAGCAGATATTCACAAACACAGTTGCTAGGTTTGCTGCAGATCTTGCTGAAGAGC
  TTGTTTTTGAAGGCATCATGGAGGTGTGTCAGTTTTCATATCCTCAAACGCCTGCATCTCCACAGTGTGG
  GTCGTTTGACTTTGAAGACAAAGTAGTGAAGTTGTATGCAAAAGATTTGTCTGAATCTGTAATACAGGAA
  GCATTCATTGAGCTATCACAAGTTGATGTGACCTTTACAACAAAGGCAGCAGTTAGTGTCTCTACGGATA
  ATATCAAGTATGTGAGTGCAGAAAGTGTAGTGCCATCGACACAGGCTGTCACGTTTTCCCCTTCTTTTCA
  CAATCAAGCAATTATGGTGACAAAACCAGTGCAGGAATATAAAAAGGAATACACAGTGCAGCAGGCCTTG
  TTTTGTACTTCTGGAATTGTTACTTCTATACCGGTGCCCTTGGCAGGAAGTGCCCTTCTCCCATATCATA
  TTTCATCTACTGCATGTCAGGCCAAGGCTCATCTGTCATCTGATGATAGTAATTCAAATGGTGATTCTGC
  CCAAGTGCATATTGCCACAAAAAACAGAGAAGAAAAAGCAGCTTGTCTCAGAAATATTTGTTTACCTTCA
  GAACACAATCCAGGTAATCAGAATGATTTTAAACCAACTAATGACGATATTGAAATGCAGAGTTCCTCAA
  AATTACCAAATGATCCTGCAATTATTAGCAACTTTTCTGCAGCAGTGGTGCATACGATAGTAAATGAAAC
  TTTAGAGTCAATGACATCATTGGAAGTTACAAAAATGGTTGATGAACGTACAGATTATTTAACTAAATCT
  TTAAAGGAGAAAACCCCTCCATTTTCCCACTGTGATCAGGCAGTGCTGCAATGCAGTGAAGCTAGTAGCA
  ATAAGGACATGTTTGCTGACCGGTTATCTAAATCTATTATTAAACATTCCATAGATAAGAGCAAATCAGT
  GATCCCAAATATAGATAAAAATGCAGTATACAAGGAAAGCTTGCCTGTTTCTGGAGAAGAATCACAGTTG
  ACACCAGAAAAGTCTCCCAAATTTCCTGACTCTCAGAATCAGTTAACTCACTGCTCACTTTCAGCTGCAA
  AGGATTGTGTTCCAGAATGTAAAGTTTCTATGGTTCATGGATCCTCCCTAGAGACACTGCCATCTTGTCC
  AGCTGTGACAGGTCAGAAATCTGACTTGAAGGAATCTGCTAAGGATCAACCACTGAAAAAGCATAACTTG
  AATAGTACATCACTTGAGGCCTTGTCTTTTGGACAGGAAAACCCCTTTCCTCATTCACATACTTTCTCAT
  CTACAGCACTTACCTGTGTAGATGGTTTGCATGTGGAAGATAAACAGAAAGTCAGAGACAGAAATGTAAT
  ACCTGATACTCCTCCATCAACTCCTCTAGTACCATCCCGGGCTAGTTCTGAATGGGATATCAAGAAGTTA
  ACTAAAAAGCTCAAGGGAGAATTAGCCAAAGAGTTTGCACCTGCTACACCACCTTCTACTCCACACAACT
  CATCTGTTGGTAGTTTGTCTGAGAATGAACAAAATACTATAGAAAAAGAAGAGTTCATGTTGAAACTCAT
  GCGATCTCTTTCTGAAGAAGTTGAGAGTAGTGAAAGTGGAGAGCTCCCAGAAGTGGATGTGAAGTCGGAG
  CACTCAGGGAAGAAGGTTCAGTTTGCAGAAGCATTAGCTACACACATCCTTTCTCTTGCAACTGAAATGG
  CAGCTTCCCATTTAGATAACAAAATAATTCAAGAACCCAAGGTTAAAAACCCTTGCTTAAATGTGCAAAG
  TCAAAGAAGTGTGTCGCCTACTTTTTTAAACCCCTCAGACGAAAATTTGAAAACATTATGCAATTTTGCG
  GGTGATCTGGCAGCAGAAGTCATTACAGAAGCTGAGAAAATAGCAAAAGTCCGAAATTGTATGCTTTTCA
  AGCAAAAGAAGAACAGTTGTTATGCTGATGGTGACGAAGATTATAAAGTAGAAGAGAAGTTGGATATAGA
  GGCTGTAGTGCACCCAAGAGAAGTGGATCCGTTTATTCTTTCATTACCACCAAGTTCTTGTATGTCAGGT
  CTGATGTATAAGTATCCCAGCTGTGAAAGTGTGACAGATGAATATGCAGGTCACCTTATTCAGATACTAA
  AACAGGAAGGTGGTAATAGTGAGTTGATAATGGATCAGTATGCCAATAGGCTTGCCTACCGATCTGTTAA
  ATCAGGATTACAGGAAGCAGCTAAGACAACCAAAGTGCAGTGCAACTCAAGAATGTTCCCTGTGCCAAGT
  TCACAAGTGAAAACAAACAAGGAACTGTTAATGTTTTCAAACAAAGAGCACCACCAAGAAGCAGACAAAA
  AGAGACAAAGTAAAAGAAATGAAGGTTACTTTTGTAAAAATCAAACTTGTGAAAGGACCCTGGATCCATA
  TAGAAATGAGGTCTCCCAACTGTATAGTTTTTCAACCTCTCTGGTTCACAGCATAACAAAAGATGCTAAG
  GAAGAGTTGACAGCCTCTCTAGTTGGCCTACCAAAATCCTTAACAGATTCTTGCTTGTTTGAAAAATCTG
  GATATGAAGAAGATAATGAGTGTCACGTTACACCAGAATTGCCTAAGTCTCTTCAGCCTTCCTCACAAAA
  TCACAGGTTTTACCACAGCACTGGCAGTTTAAATGGATATGGTTGTGGAGACAATGTTGTTCAAGCTGTA
  GAACAGTATGCCAAAAAAGTAGTGGATGACACTCTAGAGCTAACTCTAGGATCTACAGTGTTCCGAGTGT
  CTGAGACCACAAAATCAGCAGACAGGGTCACTTATGCAGAAAAGTTGTCACCTCTTACAGGTCAAGCTTG
  CAGATACTGTGACCTTAAAGAACTCCACAATTGCACTGGAAATTCATCTCAGCACTTTTTCAGACAGGGT
  TCTCTCGCCAGTAGTAAGCCAGCTTCTAATCCAAAATTTAGCAGCCGCTATCAGAAATCTAGGATTTTTC
  ATCTCAGTGTCCCTCAGATTCATGTTAATCTTGATAAGAAGGCAGTGCTTGCTGAGAAGATAGTTGCTGA
  AGCCATTGAAAAAGCTGAGCGAGAGCTGAGCAGTACCAGCCTGGCAGCCGACAGTGGGATCGGACAGGAG
  GGTGCCAGCTTTGCTGAAAGCCTTGCCACAGAAACCATGACAGCAGCTGTCACAAATGTTGGGCATGCTG
  TTAGCAGTTCAAAAGAAATAGAAGACTTTCAGTCAACCGAGTCTGTCAGTAGCCAGCAGATGAACCTCAG
  TATTGGTGATGACAGCACTGGTAGCTGGTCCAATTTAAGTTTTGAAGATGAACACCAAGATGAAAGCAGC
  AGTTTTCATCATCTAAGTGAAAGTAATGGTAACAGCAGTAGCTGGAGCAGTCTTGGTTTAGAAGGAGATT
  TGTATGAGGACAATTTATCCTTTCCAACATCAGACAGTGATGGACCAGATGATAAAGATGAAGAGCATGA
  GGACGAAGTAGAAGGTTTGGGGCAAGATGGAAAGACACTGCTAATTACGAATATTGACATGGAGCCATGC
  ACGGTAGACCCCCAGCTAAGGATTATTCTTCAGTGGCTCATTGCCTCTGAGGCTGAAGTTGCAGAACTTT
  ATTTTCATGACTCTGCAAATAAGGAGTTTATGCTACTTTCAAAACAATTACAAGAGAAAGGATGGAAAGT
  GGGAGACCTCCTGCAGGCTGTGCTTCAATACTATGAAGTGATGGAAAAAGCTTCCAGTGAGGAGAGATGC
  AAGTCGCTGTTTGATTGGCTCTTGGAAAATGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220532 representing NM_016248
  Red=Cloning site Green=Tags(s)
  
  MATFRNNHMKTKASVRKSFSEDVFQSVKSLLQSQKELCSVTAEDCLQQDEHANLTEVTFLGFNEETDAAH
  IQDLAAVSLELPDILNSLHFCSLNENEIICMKNINKPLDISSDPLNQSHPSGMLCVMRVSPTSPRLRIDF
  IFSLLSKYATGIRYTLDTFLHQKHQLETTDEDDDDTNQSVSSIEDDFVTAFEHLEEEETSKPYNDGMNIT
  VLRSQCDAASQTVTGHHLETHDLKILISSGQQKSLAKPSTSSVNVLGHKELPSVKTSVTTSISEPWTQRS
  FYRSSNASDKDSDLQKTFFSSSPAYSSESECSSPSPVIFLDEEGYQKSLKAKLELPKIPVMKDDIEDSDS
  EVSEFFDSFDQFDELEQTLETCLFNKDPVIGKSSQRKGHKHGKSCMNPQKFKFDRPALPANVRKPTPRKP
  ESPYGNLCDAPDSPRPVKASREDSGLFSPIRSSAFSPLGGCTPAECFCQTDIGGDRIHENHDSVYYTYED
  YAKSISCEVLGSVLRTHHTNTLSNINSIKHGENKTVTFKHGNLDQKNKSKNKSLMIKDSIQKFAADLVEK
  SFGSAFKDLQKGVSSCTNALYHLAIKLTSSVLQMAFDELRRQRAFSLKERAISGLANFLVSEALSNALKD
  LQYVKKQIFTNTVARFAADLAEELVFEGIMEVCQFSYPQTPASPQCGSFDFEDKVVKLYAKDLSESVIQE
  AFIELSQVDVTFTTKAAVSVSTDNIKYVSAESVVPSTQAVTFSPSFHNQAIMVTKPVQEYKKEYTVQQAL
  FCTSGIVTSIPVPLAGSALLPYHISSTACQAKAHLSSDDSNSNGDSAQVHIATKNREEKAACLRNICLPS
  EHNPGNQNDFKPTNDDIEMQSSSKLPNDPAIISNFSAAVVHTIVNETLESMTSLEVTKMVDERTDYLTKS
  LKEKTPPFSHCDQAVLQCSEASSNKDMFADRLSKSIIKHSIDKSKSVIPNIDKNAVYKESLPVSGEESQL
  TPEKSPKFPDSQNQLTHCSLSAAKDCVPECKVSMVHGSSLETLPSCPAVTGQKSDLKESAKDQPLKKHNL
  NSTSLEALSFGQENPFPHSHTFSSTALTCVDGLHVEDKQKVRDRNVIPDTPPSTPLVPSRASSEWDIKKL
  TKKLKGELAKEFAPATPPSTPHNSSVGSLSENEQNTIEKEEFMLKLMRSLSEEVESSESGELPEVDVKSE
  HSGKKVQFAEALATHILSLATEMAASHLDNKIIQEPKVKNPCLNVQSQRSVSPTFLNPSDENLKTLCNFA
  GDLAAEVITEAEKIAKVRNCMLFKQKKNSCYADGDEDYKVEEKLDIEAVVHPREVDPFILSLPPSSCMSG
  LMYKYPSCESVTDEYAGHLIQILKQEGGNSELIMDQYANRLAYRSVKSGLQEAAKTTKVQCNSRMFPVPS
  SQVKTNKELLMFSNKEHHQEADKKRQSKRNEGYFCKNQTCERTLDPYRNEVSQLYSFSTSLVHSITKDAK
  EELTASLVGLPKSLTDSCLFEKSGYEEDNECHVTPELPKSLQPSSQNHRFYHSTGSLNGYGCGDNVVQAV
  EQYAKKVVDDTLELTLGSTVFRVSETTKSADRVTYAEKLSPLTGQACRYCDLKELHNCTGNSSQHFFRQG
  SLASSKPASNPKFSSRYQKSRIFHLSVPQIHVNLDKKAVLAEKIVAEAIEKAERELSSTSLAADSGIGQE
  GASFAESLATETMTAAVTNVGHAVSSSKEIEDFQSTESVSSQQMNLSIGDDSTGSWSNLSFEDEHQDESS
  SFHHLSESNGNSSSWSSLGLEGDLYEDNLSFPTSDSDGPDDKDEEHEDEVEGLGQDGKTLLITNIDMEPC
  TVDPQLRIILQWLIASEAEVAELYFHDSANKEFMLLSKQLQEKGWKVGDLLQAVLQYYEVMEKASSEERC
  KSLFDWLLENA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016248
• ORF Size: 5703 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016248.4
• RefSeq Size: 9919 bp
• RefSeq ORF: 5706 bp
• Locus ID: 11215
• UniProt ID: Q9UKA4
• Cytogenetics: 13q14.11
• Protein Families: Druggable Genome
• MW: 210.3 kDa
• Gene Summary: The A-kinase anchor proteins (AKAPs) are a group of structurally diverse proteins, which have the common function of binding to the regulatory subunit of protein kinase A (PKA) and confining the holoenzyme to discrete locations within the cell. This gene encodes a member of the AKAP family. The encoded protein is expressed at high levels throughout spermatogenesis and in mature sperm. It binds the RI and RII subunits of PKA in testis. It may serve a function in cell cycle control of both somatic cells and germ cells in addition to its putative role in spermatogenesis and sperm function. [provided by RefSeq, Jul 2008]