EYA1 (NM_000503) Human Untagged Clone

CAT#: SC309058

EYA1 (untagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EYA1
• Synonyms: BOP; BOR; BOS1; OFC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_000503 edited
  GCCACCATGGAAATGCAGGATCTAACCAGCCCGCATAGCCGTCTGAGTGGTAGTAGTGAA
  TCCCCCAGTGGCCCCAAACTCGGTAACTCTCATATAAATAGTAATTCCATGACTCCCAAT
  GGCACCGAAGTTAAAACAGAGCCAATGAGCAGCAGTGAAACAGCTTCAACGACAGCCGAC
  GGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGGAGCAGTAGTTTCAGCCCACGACCA
  ACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCATACCCACATATTCTC
  CCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGGAATG
  CAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCA
  TATGGTGCATTGTGGGCAGGCATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCT
  GGACAGACAGGATTTCTCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCA
  CCATACAGCTACCAGATGCAAGGTAGCAGTTTTACAACATCATCAGGAATATATACAGGA
  AATAATTCACTCACAAATTCCTCTGGATTTAATAGTTCACAGCAGGACTATCCGTCTTAT
  CCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCACCGTATCCAGCACAT
  TATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCAGCTT
  CAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGC
  ACAATCCACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGT
  TCAGATGGGAAATCACGTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGAT
  TCTGATCTTGAGAGAGTGTTCATCTGGGACTTGGATGAGACAATCATTGTTTTCCACTCC
  TTGCTTACTGGGTCCTACGCCAACAGATATGGGAGGGATCCACCCACTTCAGTTTCCCTT
  GGACTGCGAATGGAAGAAATGATTTTCAACTTGGCAGACACACATTTATTTTTTAATGAC
  TTAGAAGAATGTGACCAAGTCCATATAGATGATGTTTCTTCAGATGATAACGGACAGGAC
  CTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAGTGCTAACTTA
  TGTTTGGCAACTGGTGTACGGGGCGGTGTGGACTGGATGAGAAAGTTGGCCTTCCGCTAC
  AGACGGGTAAAAGAGATCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCA
  GCTAAGAGGGAAGCCTGGCTGCAGTTGAGGGCCGAAATTGAAGCCCTGACCGACTCCTGG
  TTGACACTGGCCCTGAAAGCACTCTCGCTCATTCACTCCCGGACAAACTGTGTGAATATT
  TTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGCTGTATGGGTTAGGA
  ATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTGTTTT
  GAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGGAGATGGTGTA
  GAAGAAGAACAAGGAGCAAAAAAGCACGCGATGCCCTTCTGGAGGATCTCCAGCCACTCG
  GACCTCATGGCCCTGCACCATGCCTTGGAACTGGAGTACCTGTAA
  
• Restriction Sites: Please inquire
• ACCN: NM_000503
• Insert Size: 2000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000503.3, NP_000494.2
• RefSeq Size: 4326 bp
• RefSeq ORF: 1779 bp
• Locus ID: 2138
• UniProt ID: Q99502
• Cytogenetics: 8q13.3
• Domains: Hydrolase
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• Gene Summary: This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]
  Transcript Variant: This variant (EYA1C) represents the longest transcript and encodes the longest isoform (1). Variants EYA1C and EYA1B encode the same isoform.