GANP (MCM3AP) (NM_003906) Human Untagged Clone

CAT#: SC308999

MCM3AP (untagged)-Human minichromosome maintenance complex component 3 associated protein (MCM3AP)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GANP
• Synonyms: GANP; MAP80; PNRIID; SAC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308999 representing NM_003906.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAACCCAACTAATCCTTTCAGTGGGCAGCAGCCTAGTGCTTTTTCGGCGTCTTCTAGTAATGTAGGA
  ACACTTCCATCTAAGCCGCCATTTCGATTTGGTCAACCTTCTCTTTTTGGACAAAACAGTACCTTATCT
  GGGAAGAGCTCGGGATTTTCACAGGTATCCAGCTTTCCAGCGTCTTCTGGAGTAAGTCATTCCTCTTCA
  GTGCAAACATTAGGGTTCACCCAAACCTCAAGTGTTGGACCCTTTTCTGGACTTGAGCACACTTCCACC
  TTTGTGGCTACCTCTGGGCCTTCAAGTTCATCTGTGCTGGGAAACACAGGATTTAGTTTTAAATCACCC
  ACCAGTGTTGGGGCTTTCCCAAGCACTTCTGCTTTTGGACAAGAAGCTGGAGAAATAGTGAACTCTGGT
  TTTGGGAAAACAGAATTCAGCTTTAAACCTCTGGAAAATGCAGTGTTCAAACCAATACTGGGGGCTGAA
  TCTGAGCCAGAGAAAACCCAGAGCCAAATTGCTTCTGGGTTTTTTACATTTTCCCACCCAATTAGTAGT
  GCACCTGGAGGCCTGGCCCCTTTCTCTTTTCCTCAAGTAACAAGTAGTTCAGCTACCACTTCAAATTTT
  ACCTTTTCAAAACCTGTTAGTAGTAATAATTCATTATCTGCCTTTACCCCTGCTTTGTCAAACCAAAAT
  GTAGAGGAAGAGAAGAGAGGACCTAAGTCAATATTTGGAAGTTCTAATAATAGCTTCAGTAGCTTCCCT
  GTATCATCTGCGGTTTTGGGCGAACCTTTCCAGGCTAGCAAAGCAGGTGTCAGGCAGGGGTGTGAAGAA
  GCTGTTTCCCAGGTGGAACCACTTCCCAGCCTAATGAAAGGACTGAAAAGGAAGGAGGACCAGGATCGC
  TCCCCAAGGAGACATGGCCACGAGCCAGCAGAAGATTCGGATCCTCTGTCCCGGGGCGATCATCCTCCA
  GACAAACGACCTGTCCGCCTGAATCGACCCCGGGGAGGTACTTTATTTGGTCGGACGATACAGGATGTT
  TTCAAAAGCAATAAGGAAGTAGGTCGTCTGGGCAACAAGGAGGCCAAAAAGGAAACTGGCTTTGTTGAG
  TCTGCAGAAAGTGACCACATGGCTATCCCAGGAGGGAATCAGTCTGTCCTGGCACCTTCCCGGATTCCA
  GGTGTGAATAAAGAGGAAGAAACTGAAAGTAGAGAGAAGAAAGAAGATTCTCTAAGAGGAACTCCGGCG
  CGTCAGAGTAACAGAAGCGAGAGCACAGACAGTCTTGGGGGCTTGTCTCCCTCTGAAGTCACAGCCATC
  CAGTGCAAGAACATCCCTGACTACCTCAACGACAGGACCATTCTGGAGAACCATTTTGGCAAAATTGCT
  AAAGTGCAGCGCATCTTTACCAGGCGCAGCAAAAAGCTTGCAGTGGTACATTTCTTTGATCATGCATCT
  GCAGCCCTGGCTAGAAAGAAGGGGAAAAGTTTGCATAAAGACATGGCTATCTTTTGGCACAGGAAGAAA
  ATAAGCCCCAATAAGAAACCCTTTTCCCTGAAGGAGAAGAAACCAGGTGACGGTGAAGTCAGCCCGAGC
  ACAGAGGATGCACCCTTTCAGCACTCTCCTCTTGGCAAGGCCGCAGGGAGGACTGGTGCTAGCAGCCTC
  CTGAATAAAAGCTCTCCAGTGAAGAAGCCAAGTCTTCTAAAGGCCCACCAATTCGAGGGAGACTCTTTT
  GACTCAGCCTCCGAGGGCTCCGAGGGCCTCGGGCCATGTGTGCTCTCCCTCAGTACCCTGATAGGCACT
  GTGGCTGAGACATCCAAGGAGAAGTACCGCCTGCTTGACCAGAGAGACAGGATCATGCGGCAAGCTCGG
  GTGAAGAGAACCGATCTGGACAAAGCGAGGACTTTTGTTGGCACCTGCCTGGATATGTGTCCTGAGAAG
  GAGAGGTACATGCGGGAGACCCGTAGCCAGCTGAGCGTGTTCGAAGTGGTCCCAGGGACTGACCAGGTG
  GACCACGCAGCAGCTGTGAAAGAGTACAGTCGGTCCTCGGCGGATCAGGAGGAGCCCCTGCCCCACGAG
  CTGCGGCCCTTGCCAGTGCTCAGCAGGACCATGGACTACCTGGTGACCCAGATCATGGACCAGAAGGAG
  GGCAGCCTGCGGGATTGGTATGACTTCGTGTGGAACCGCACGCGTGGCATACGGAAGGATATCACGCAG
  CAGCACCTCTGTGACCCCCTGACGGTGTCCCTGATTGAGAAGTGCACCCGGTTTCACATCCACTGTGCC
  CACTTCATGTGTGAGGAGCCCATGTCCTCCTTTGATGCCAAGATCAATAATGAGAACATGACCAAGTGC
  CTGCAGAGCCTGAAGGAGATGTACCAGGACCTGAGAAACAAGGGTGTCTTCTGTGCCAGCGAAGCGGAG
  TTCCAGGGCTACAATGTTCTGCTCAGTCTCAACAAGGGAGACATCCTAAGAGAAGTACAACAGTTCCAT
  CCTGCTGTTAGAAACTCATCTGAGGTGAAATTTGCTGTTCAGGCTTTTGCTGCATTGAACAGTAATAAT
  TTTGTGAGATTTTTCAAACTGGTCCAGTCAGCTTCTTACCTGAACGCTTGTCTTTTACACTGTTACTTC
  AGTCAGATCCGCAAGGATGCTCTCCGGGCGCTCAACTTTGCGTACACGGTGAGCACACAGCGATCTACC
  ATCTTTCCCCTGGATGGTGTGGTGCGCATGCTGCTGTTCAGAGACTGTGAAGAGGCCACCGACTTCCTC
  ACCTGCCACGGCCTCACCGTTTCCGACGGCTGTGTGGAGCTGAACCGGTCTGCATTCCTGGAACCAGAG
  GGATTATCCAAGACCAGGAAGTCGGTGTTTATTACTAGGAAGCTGACGGTGTCAGTCGGGGAAATTGTG
  AACGGAGGGCCATTGCCCCCCGTCCCTCGTCATACCCCTGTGTGCAGCTTCAACTCCCAGAACAAGTAC
  ATCGGGGAGAGCCTGGCCGCGGAGCTGCCCGTCAGCACCCAGAGACCCGGCTCCGACACAGTGGGCGGA
  GGGAGAGGAGAGGAGTGTGGTGTAGAGCCGGATGCACCCCTGTCCAGTCTCCCACAGTCTCTACCAGCC
  CCTGCGCCCTCACCAGTGCCTCTGCCTCCTGTCCTGGCACTGACCCCGTCTGTGGCGCCCAGCCTCTTC
  CAGCTGTCTGTGCAGCCTGAACCACCGCCTCCAGAGCCCGTGCCCATGTACTCTGACGAGGACCTGGCG
  CAGGTGGTGGACGAGCTCATCCAGGAGGCCCTGCAGAGGGACTGTGAGGAAGTTGGCTCTGCGGGTGCT
  GCCTACGCAGCTGCCGCCCTGGGTGTTTCTAATGCTGCTATGGAGGATTTGTTAACAGCTGCAACCACG
  GGCATTTTGAGGCACATTGCAGCTGAAGAAGTGTCTAAGGAAAGAGAGCGAAGGGAGCAGGAGAGGCAG
  CGGGCTGAAGAGGAAAGGTTGAAACAAGAGAGAGAGCTGGTGTTAAGTGAGCTGAGCCAGGGCCTGGCC
  GTGGAGCTGATGGAACGCGTGATGATGGAGTTTGTGAGGGAAACCTGCTCCCAGGAGTTGAAGAATGCA
  GTAGAGACAGACCAGAGGGTCCGTGTGGCCCGTTGCTGTGAGGATGTCTGTGCCCACTTAGTGGACTTG
  TTTCTCGTGGAGGAAATCTTCCAGACTGCAAAGGAGACCCTCCAGGAGCTTCAGTGCTTCTGCAAGTAT
  CTACAGCGGTGGAGGGAAGCTGTCACAGCCCGCAAGAAACTGAGGCGCCAAATGCGGGCTTTCCCTGCT
  GCGCCCTGCTGCGTGGACGTGAGCGACCGGCTGAGGGCGCTGGCGCCCAGCGCAGAGTGCCCCATTGCT
  GAAGAGAACCTGGCCAGGGGCCTCCTGGACCTGGGCCATGCAGGGAGATTGGGCATCTCCTGCACCAGG
  TTAAGGCGGCTCAGAAACAAGACAGCTCACCAGATGAAGGTTCAGCACTTCTACCAGCAGCTGCTGAGT
  GATGTGGCATGGGCGTCTCTGGACCTGCCATCCCTCGTGGCTGAGCACCTCCCTGGGAGGCAGGAGCAT
  GTGTTTTGGAAGCTGGTGCTGGTGTTGCCGGATGTAGAGGAGCAGTCCCCAGAGAGTTGTGGCAGAATT
  CTAGCAAATTGGTTAAAAGTCAAGTTCATGGGAGATGAAGGCTCAGTGGATGACACATCCAGCGATGCT
  GGTGGGATTCAGACGCTTTCGCTTTTCAACTCACTTAGCAGCAAAGGGGATCAGATGATTTCTGTTAAC
  GTGTGTATAAAGGTGGCCCATGGCGCCCTCAGTGATGGTGCCATTGATGCTGTGGAGACACAGAAGGAC
  CTCCTGGGAGCCAGTGGGCTCATGCTGCTGCTTCCCCCCAAAATGAAGAGTGAGGACATGGCAGAGGAG
  GACGTGTACTGGCTGTCGGCCTTGCTGCAGCTCAAGCAGCTCCTGCAGGCTAAGCCCTTCCAGCCTGCG
  CTTCCTCTGGTGGTTCTTGTGCCTAGCCCAGGAGGGGACGCCGTTGAGAAGGAAGTAGAAGATGGTCTG
  ATGCTACAGGACTTGGTTTCAGCTAAGCTGATTTCAGATTACACTGTTACCGAGATCCCTGATACCATT
  AATGATCTACAAGGTTCAACTAAGGTTTTGCAAGCAGTGCAGTGGCTGGTTTCCCACTGCCCCCATTCC
  CTTGACCTCTGCTGCCAGACTCTCATTCAGTACGTCGAAGACGGGATTGGCCATGAGTTTAGTGGCCGC
  TTTTTCCATGACAGAAGAGAGAGGCGTCTGGGCGGTCTTGCTTCTCAGGAGCCTGGCGCCATCATTGAG
  CTGTTTAACAGTGTGCTGCAGTTCCTGGCTTCTGTGGTGTCCTCTGAACAGCTGTGTGACCTGTCCTGG
  CCTGTCACTGAGTTTGCTGAGGCAGGGGGCAGCCGGCTGCTTCCTCACCTGCACTGGAATGCCCCAGAG
  CACCTGGCCTGGCTGAAGCAGGCTGTGCTCGGGTTCCAGCTTCCGCAGATGGACCTTCCACCCCTGGGG
  GCCCCCTGGCTCCCCGTGTGCTCCATGGTTGTCCAGTACGCCTCCCAGATCCCCAGCTCACGCCAGACA
  CAGCCTGTCCTCCAGTCCCAGGTGGAGAACCTGCTCCACAGAACCTACTGTAGGTGGAAGAGCAAGAGT
  CCCTCCCCAGTCCATGGGGCAGGCCCCTCGGTCATGGAGATCCCATGGGATGATCTTATCGCCTTGTGT
  ATCAACCACAAGCTGAGAGACTGGACGCCCCCCCGGCTTCCTGTTACATCAGAGGCGCTGAGTGAAGAT
  GGTCAGATATGTGTGTATTTTTTTAAAAACGATTTGAAAAAATATGATGTTCCTTTGTCGTGGGAACAA
  GCCAGGTTGCAGACGCAGAAGGAGCTACAGCTGAGAGAGGGACGTTTGGCAATAAAGCCTTTTCATCCT
  TCTGCAAACAATTTTCCCATACCATTGCTTCACATGCACCGTAACTGGAAGAGGAGCACAGAGTGTGCT
  CAAGAGGGGAGGATTCCCAGCACAGAGGATCTGATGCGAGGAGCTTCTGCTGAGGAGCTCTTGGCGCAG
  TGTTTGTCGAGCAGTCTGCTGCTGGAGAAAGAAGAGAACAAGAGGTTTGAAGATCAGCTTCAGCAATGG
  TTGTCTGAAGACTCAGGAGCATTTACGGATTTAACTTCCCTTCCCCTCTATCTTCCTCAGACTCTAGTG
  TCTCTTTCTCACACTATTGAACCTGTGATGAAAACATCTGTAACTACTAGCCCACAGAGTGACATGATG
  AGGGAGCAACTGCAGCTGTCAGAGGCGACAGGAACGTGTCTAGGCGAACGACTAAAGCACCTGGAAAGG
  CTGATCCGGAGTTCAAGGGAAGAGGAAGTTGCCTCTGAGCTCCATCTCTCTGCGCTGCTAGACATGGTG
  GACATTTGA
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGAT
  ATCCTGGATTACAAGGATGACGACGATAAGGTTTAA
  
• Restriction Sites: SgfI-RsrII
• ACCN: NM_003906
• Insert Size: 5943 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003906.4
• RefSeq Size: 6198 bp
• RefSeq ORF: 5943 bp
• Locus ID: 8888
• UniProt ID: O60318
• Cytogenetics: 21q22.3
• Domains: SAC3_GANP
• MW: 218.4 kDa
• Gene Summary: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]