GANP (MCM3AP) (NM_003906) Human Tagged ORF Clone

CAT#: RC218026

MCM3AP (Myc-DDK-tagged)-Human minichromosome maintenance complex component 3 associated protein (MCM3AP)

Product Images

Circular map for RC218026

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GANP
• Synonyms: GANP; MAP80; PNRIID; SAC3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218026 representing NM_003906
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACCCAACTAATCCTTTCAGTGGGCAGCAGCCTAGTGCTTTTTCGGCGTCTTCTAGTAATGTAGGAA
  CACTTCCATCTAAGCCGCCATTTCGATTTGGTCAACCTTCTCTTTTTGGACAAAACAGTACCTTATCTGG
  GAAGAGCTCGGGATTTTCACAGGTATCCAGCTTTCCAGCGTCTTCTGGAGTAAGTCATTCCTCTTCAGTG
  CAAACATTAGGGTTCACCCAAACCTCAAGTGTTGGACCCTTTTCTGGACTTGAGCACACTTCCACCTTTG
  TGGCTACCTCTGGGCCTTCAAGTTTATCTGTGCTGGGAAACACAGGATTTAGTTTTAAATCACCCACCAG
  TGTTGGGGCTTTCCCAAGCACTTCTGCTTTTGGACAAGAAGCTGGAGAAATAGTGAACTCTGGTTTTGGG
  AAAACAGAATTCAGCTTTAAACCTCTGGAAAATGCAGTGTTCAAACCAATACTGGGGGCTGAATCTGAGC
  CAGAGAAAACCCAGAGCCAAATTGCTTCTGGGTTTTTTACATTTTCCCACCCAATTAGTAGTGCACCTGG
  AGGCCTGGCCCCTTTCTCTTTTCCTCAAGTAACAAGTAGTTCAGCTACCACTTCAAATTTTACCTTTTCA
  AAACCTGTTAGTAGTAATAATTCATTATCTGCCTTTACCCCTGCTTTGTCAAACCAAAATGTAGAGGAAG
  AGAAGAGAGGACCTAAGTCAATATTTGGAAGTTCTAATAATAGCTTCAGTAGCTTCCCTGTATCATCTGC
  GGTTTTGGGCGAACCTTTCCAGGCTAGCAAAGCAGGTGTCAGGCAGGGGTGTGAAGAAGCTGTTTCCCAG
  GTGGAACCACTTCCCAGCCTAATGAAAGGACTGAAAAGGAAGGAGGACCAGGATCGCTCCCCAAGGAGAC
  ATGGCCACGAGCCAGCAGAAGATTCGGATCCTCTGTCCCGGGGCGATCATCCTCCAGACAAACGACCTGT
  CCGCCTGAATCGACCCCGGGGAGGTACTTTATTTGGTCGGACGATACAGGATGTTTTCAAAAGCAATAAG
  GAAGTAGGTCGTCTGGGCAACAAGGAGGCCAAAAAGGAAACTGGCTTTGTTGAGTCTGCAGAAAGTGACC
  ACATGGCTATCCCAGGAGGGAATCAGTCTGTCCTGGCACCTTCCCGGATTCCAGGTGTGAATAAAGAGGA
  AGAAACTGAAAGTAGAGAGAAGAAAGAAGATTCTCTAAGAGGAACTCCGGCGCGTCAGAGTAACAGAAGC
  GAGAGCACAGACAGTCTTGGGGGCTTGTCTCCCTCTGAAGTCACAGCCATCCAGTGCAAGAATATCCCTG
  ACTACCTCAACGACAGGACCATTCTGGAGAACCATTTTGGCAAAATTGCTAAAGTGCAGCGCATCTTTAC
  CAGGCGCAGCAAAAAGCTTGCAGTGGTACATTTCTTTGATCATGCATCTGCAGCCCTGGCTAGAAAGAAG
  GGGAAAAGTTTGCATAAAGACATGGCTATCTTTTGGCACAGGAAGAAAATAAGCCCCAATAAGAAACCCT
  TTTCCCTGAAGGAGAAGAAACCAGGTGACGGTGAAGTCAGCCCGAGCACAGAGGATGCACCCTTTCAGCA
  CTCTCCTCTTGGCAAGGCCGCAGGGAGGACTGGTGCTAGCAGCCTCCTGAATAAAAGCTCTCCAGTGAAG
  AAGCCAAGTCTTCTAAAGGCCCACCAATTCGAGGGAGACTCTTTTGACTCAGCCTCCGAGGGCTCCGAGG
  GCCTCGGGCCATGTGTGCTCTCCCTCAGTACCCTGATAGGCACTGTGGCTGAGACATCCAAGGAGAAGTA
  CCGCCTGCTTGACCAGAGAGACAGGATCATGCGGCAAGCTCGGGTGAAGAGAACCGATCTGGACAAAGCG
  AGGACTTTTGTTGGCACCTGCCTGGATATGTGTCCTGAGAAGGAGAGGTACATGCGGGAGACCCGTAGCC
  AGCTGAGCGTGTTCGAAGTGGTCCCAGGGACTGACCAGGTGGACCACGCAGCAGCTGTGAAAGAGTACAG
  TCGGTCCTCGGCGGATCAGGAGGAGCCCCTGCCCCACGAGCTGCGGCCCTTGCCAGTGCTCAGCAGGACC
  ATGGACTACCTGGTGACCCAGATCATGGACCAGAAGGAGGGCAGCCTGCGGGATTGGTATGACTTCGTGT
  GGAACCGCACGCGTGGCATACGGAAGGATATCACGCAGCAGCACCTCTGTGACCCCCTGACGGTGTCCCT
  GATTGAGAAGTGCACCCGGTTTCACATCCACTGTGCCCACTTCATGTGTGAGGAGCCCATGTCCTCCTTT
  GATGCCAAGATCAATAATGAGAACATGACCAAGTGCCTGCAGAGCCTGAAGGAGATGTACCAGGACCTGA
  GAAACAAGGGTGTCTTCTGTGCCAGCGAAGCGGAGTTCCAGGGCTACAATGTTCTGCTCAGTCTCAACAA
  GGGAGACATCCTAAGAGAAGTACAACAGTTCCATCCTGCTGTTAGAAACTCATCTGAGGTGAAATTTGCT
  GTTCAGGCTTTTGCTGCATTGAACAGTAATAATTTTGTGAGATTTTTCAAACTGGTCCAGTCAGCTTCTT
  ACCTGAACGCTTGTCTTTTACACTGTTACTTCAGTCAGATCCGCAAGGATGCTCTCCGGGCGCTCAACTT
  TGCGTACACGGTGAGCACACAGCGATCTACCATCTTTCCCCTGGATGGTGTGGTGCGCATGCTGCTGTTC
  AGAGACTGTGAAGAGGCCACCGACTTCCTCACCTGCCACGGCCTCACCGTTTCCGACGGCTGTGTGGAGC
  TGAACCGGTCTGCATTCCTGGAACCAGAGGGATTATCCAAGACCAGGAAGTCGGTGTTTATTACTAGGAA
  GCTGACGGTGTCAGTCGGGGAAATTGTGAACGGAGGGCCATTGCCCCCCGTCCCTCGTCATACCCCTGTG
  TGCAGCTTCAACTCCCAGAACAAGTACATCGGGGAGAGCCTGGCCGCGGAGCTGCCCGTCAGCACCCAGA
  GACCCGGCTCCGACACAGTGGGCGGAGGGAGAGGAGAGGAGTGTGGTGTAGAGCCGGATGCACCCCTGTC
  CAGTCTCCCACAGTCTCTACCAGCCCCTGCGCCCTCACCAGTGCCTCTGCCTCCTGTCCTGGCACTGACC
  CCGTCTGTGGCGCCCAGCCTCTTCCAGCTGTCTGTGCAGCCTGAACCACCGCCTCCAGAGCCCGTGCCCA
  TGTACTCTGACGAGGACCTGGCGCAGGTGGTGGACGAGCTCATCCAGGAGGCCCTGCAGAGGGACTGTGA
  GGAAGTTGGCTCTGCGGGTGCTGCCTACGCAGCTGCCGCCCTGGGTGTTTCTAATGCTGCTATGGAGGAT
  TTGTTAACAGCTGCAACCACGGGCATTTTGAGGCACATTGCAGCTGAAGAAGTGTCTAAGGAAAGAGAGC
  GAAGGGAGCAGGAGAGGCAGCGGGCTGAAGAGGAAAGGTTGAAACAAGAGAGAGAGCTGGTGTTAAGTGA
  GCTGAGCCAGGGCCTGGCCGTGGAGCTGATGGAACGCGTGATGATGGAGTTTGTGAGGGAAACCTGCTCC
  CAGGAGTTGAAGAATGCAGTAGAGACAGACCAGAGGGTCCGTGTGGCCCGTTGCTGTGAGGATGTCTGTG
  CCCACTTAGTGGACTTGTTTCTCGTGGAGGAAATCTTCCAGACTGCAAAGGAGACCCTCCAGGAGCTTCA
  GTGCTTCTGCAAGTATCTACAGCGGTGGAGGGAAGCTGTCACAGCCCGCAAGAAACTGAGGCGCCAAATG
  CGGGCTTTCCCTGCTGCGCCCTGCTGCGTGGACGTGAGCGACCGGCTGAGGGCGCTGGCGCCCAGCGCAG
  AGTGCCCCATTGCTGAAGAGAACCTGGCCAGGGGCCTCCTGGACCTGGGCCATGCAGGGAGATTGGGCAT
  CTCCTGCACCAGGTTAAGGCGGCTCAGAAACAAGACAGCTCACCAGATGAAGGTTCAGCACTTCTACCAG
  CAGCTGCTGAGTGATGTGGCATGGGCGTCTCTGGACCTGCCATCCCTCGTGGCTGAGCACCTCCCTGGGA
  GGCAGGAGCATGTGTTTTGGAAGCTGGTGCTGGTGTTGCCGGATGTAGAGGAGCAGTCCCCAGAGAGTTG
  TGGCAGAATTCTAGCAAATTGGTTAAAAGTCAAGTTCATGGGAGATGAAGGCTCAGTGGATGACACATCC
  AGCGATGCTGGTGGGATTCAGACGCTTTCGCTTTTCAACTCACTTAGCAGCAAAGGGGATCAGATGATTT
  CTGTTAACGTGTGTATAAAGGTGGCCCATGGCGCCCTCAGTGATGGTGCCATTGATGCTGTGGAGACACA
  GAAGGACCTCCTGGGAGCCAGTGGGCTCATGCTGCTGCTTCCCCCCAAAATGAAGAGTGAGGACATGGCA
  GAGGAGGACGTGTACTGGCTGTCGGCCTTGCTGCAGCTCAAGCAGCTCCTGCAGGCTAAGCCCTTCCAGC
  CTGCGCTTCCTCTGGTGGTTCTTGTGCCTAGCCCAGGAGGGGACGCCGTTGAGAAGGAAGTAGAAGATGG
  TCTGATGCTACAGGACTTGGTTTCAGCTAAGCTGATTTCAGATTACACTGTTACCGAGATCCCTGATACC
  ATTAATGATCTACAAGGTTCAACTAAGGTTTTGCAAGCAGTGCAGTGGCTGGTTTCCCACTGCCCCCATT
  CCCTTGACCTCTGCTGCCAGACTCTCATTCAGTACGTCGAAGACGGGATTGGCCATGAGTTTAGTGGCCG
  CTTTTTCCATGACAGAAGAGAGAGGCGTCTGGGCGGTCTTGCTTCTCAGGAGCCTGGCGCCATCATTGAG
  CTGTTTAACAGTGTGCTGCAGTTCCTGGCTTCTGTGGTGTCCTCTGAACAGCTGTGTGACCTGTCCTGGC
  CTGTCACTGAGTTTGCTGAGGCAGGGGGCAGCCGGCTGCTTCCTCACCTGCACTGGAATGCCCCAGAGCA
  CCTGGCCTGGCTGAAGCAGGCTGTGCTCGGGTTCCAGCTTCCGCAGATGGACCTTCCACCCCTGGGGGCC
  CCCTGGCTCCCCGTGTGCTCCATGGTTGTCCAGTACGCCTCCCAGATCCCCAGCTCACGCCAGACACAGC
  CTGTCCTCCAGTCCCAGGTGGAGAACCTGCTCCACAGAACCTACTGTAGGTGGAAGAGCAAGAGTCCCTC
  CCCAGTCCATGGGGCAGGCCCCTCGGTCATGGAGATCCCATGGGATGATCTTATCGCCTTGTGTATCAAC
  CACAAGCTGAGAGACTGGACGCCCCCCCGGCTTCCTGTTACATCAGAGGCGCTGAGTGAAGATGGTCAGA
  TATGTGTGTATTTTTTTAAAAACGATTTGAAAAAATATGATGTTCCTTTGTCGTGGGAACAAGCCAGGTT
  GCAGACGCAGAAGGAGCTACAGCTGAGAGAGGGACGTTTGGCAATAAAGCCTTTTCATCCTTCTGCAAAC
  AATTTTCCCATACCATTGCTTCACATGCACCGTAACTGGAAGAGGAGCACAGAGTGTGCTCAAGAGGGGA
  GGATTCCCAGCACAGAGGATCTGATGCGAGGAGCTTCTGCTGAGGAGCTCTTGGCGCAGTGTTTGTCGAG
  CAGTCTGCTGCTGGAGAAAGAAGAGAACAAGAGGTTTGAAGATCAGCTTCAGCAATGGTTGTCTGAAGAC
  TCAGGAGCATTTACGGATTTAACTTCCCTTCCCCTCTATCTTCCTCAGACTCTAGTGTCTCTTTCTCACA
  CTATTGAACCTGTGATGAAAACATCTGTAACTACTAGCCCACAGAGTGACATGATGAGGGAGCAACTGCA
  GCTGTCAGAGGCGACAGGAACGTGTCTAGGCGAACGACTAAAGCACCTGGAAAGGCTGATCCGGAGTTCA
  AGGGAAGAGGAAGTTGCCTCTGAGCTCCATCTCTCTGCGCTGCTAGACATGGTGGACATTAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218026 representing NM_003906
  Red=Cloning site Green=Tags(s)
  
  MNPTNPFSGQQPSAFSASSSNVGTLPSKPPFRFGQPSLFGQNSTLSGKSSGFSQVSSFPASSGVSHSSSV
  QTLGFTQTSSVGPFSGLEHTSTFVATSGPSSLSVLGNTGFSFKSPTSVGAFPSTSAFGQEAGEIVNSGFG
  KTEFSFKPLENAVFKPILGAESEPEKTQSQIASGFFTFSHPISSAPGGLAPFSFPQVTSSSATTSNFTFS
  KPVSSNNSLSAFTPALSNQNVEEEKRGPKSIFGSSNNSFSSFPVSSAVLGEPFQASKAGVRQGCEEAVSQ
  VEPLPSLMKGLKRKEDQDRSPRRHGHEPAEDSDPLSRGDHPPDKRPVRLNRPRGGTLFGRTIQDVFKSNK
  EVGRLGNKEAKKETGFVESAESDHMAIPGGNQSVLAPSRIPGVNKEEETESREKKEDSLRGTPARQSNRS
  ESTDSLGGLSPSEVTAIQCKNIPDYLNDRTILENHFGKIAKVQRIFTRRSKKLAVVHFFDHASAALARKK
  GKSLHKDMAIFWHRKKISPNKKPFSLKEKKPGDGEVSPSTEDAPFQHSPLGKAAGRTGASSLLNKSSPVK
  KPSLLKAHQFEGDSFDSASEGSEGLGPCVLSLSTLIGTVAETSKEKYRLLDQRDRIMRQARVKRTDLDKA
  RTFVGTCLDMCPEKERYMRETRSQLSVFEVVPGTDQVDHAAAVKEYSRSSADQEEPLPHELRPLPVLSRT
  MDYLVTQIMDQKEGSLRDWYDFVWNRTRGIRKDITQQHLCDPLTVSLIEKCTRFHIHCAHFMCEEPMSSF
  DAKINNENMTKCLQSLKEMYQDLRNKGVFCASEAEFQGYNVLLSLNKGDILREVQQFHPAVRNSSEVKFA
  VQAFAALNSNNFVRFFKLVQSASYLNACLLHCYFSQIRKDALRALNFAYTVSTQRSTIFPLDGVVRMLLF
  RDCEEATDFLTCHGLTVSDGCVELNRSAFLEPEGLSKTRKSVFITRKLTVSVGEIVNGGPLPPVPRHTPV
  CSFNSQNKYIGESLAAELPVSTQRPGSDTVGGGRGEECGVEPDAPLSSLPQSLPAPAPSPVPLPPVLALT
  PSVAPSLFQLSVQPEPPPPEPVPMYSDEDLAQVVDELIQEALQRDCEEVGSAGAAYAAAALGVSNAAMED
  LLTAATTGILRHIAAEEVSKERERREQERQRAEEERLKQERELVLSELSQGLAVELMERVMMEFVRETCS
  QELKNAVETDQRVRVARCCEDVCAHLVDLFLVEEIFQTAKETLQELQCFCKYLQRWREAVTARKKLRRQM
  RAFPAAPCCVDVSDRLRALAPSAECPIAEENLARGLLDLGHAGRLGISCTRLRRLRNKTAHQMKVQHFYQ
  QLLSDVAWASLDLPSLVAEHLPGRQEHVFWKLVLVLPDVEEQSPESCGRILANWLKVKFMGDEGSVDDTS
  SDAGGIQTLSLFNSLSSKGDQMISVNVCIKVAHGALSDGAIDAVETQKDLLGASGLMLLLPPKMKSEDMA
  EEDVYWLSALLQLKQLLQAKPFQPALPLVVLVPSPGGDAVEKEVEDGLMLQDLVSAKLISDYTVTEIPDT
  INDLQGSTKVLQAVQWLVSHCPHSLDLCCQTLIQYVEDGIGHEFSGRFFHDRRERRLGGLASQEPGAIIE
  LFNSVLQFLASVVSSEQLCDLSWPVTEFAEAGGSRLLPHLHWNAPEHLAWLKQAVLGFQLPQMDLPPLGA
  PWLPVCSMVVQYASQIPSSRQTQPVLQSQVENLLHRTYCRWKSKSPSPVHGAGPSVMEIPWDDLIALCIN
  HKLRDWTPPRLPVTSEALSEDGQICVYFFKNDLKKYDVPLSWEQARLQTQKELQLREGRLAIKPFHPSAN
  NFPIPLLHMHRNWKRSTECAQEGRIPSTEDLMRGASAEELLAQCLSSSLLLEKEENKRFEDQLQQWLSED
  SGAFTDLTSLPLYLPQTLVSLSHTIEPVMKTSVTTSPQSDMMREQLQLSEATGTCLGERLKHLERLIRSS
  REEEVASELHLSALLDMVDISGP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003906
• ORF Size: 5949 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003906.5
• RefSeq Size: 6113 bp
• RefSeq ORF: 5943 bp
• Locus ID: 8888
• UniProt ID: O60318
• Cytogenetics: 21q22.3
• Domains: SAC3_GANP
• MW: 218.2 kDa
• Gene Summary: The minichromosome maintenance protein 3 (MCM3) is one of the MCM proteins essential for the initiation of DNA replication. The protein encoded by this gene is a MCM3 binding protein. It was reported to have phosphorylation-dependent DNA-primase activity, which was up-regulated in antigen immunization induced germinal center. This protein was demonstrated to be an acetyltransferase that acetylates MCM3 and plays a role in DNA replication. The mutagenesis of a nuclear localization signal of MCM3 affects the binding of this protein with MCM3, suggesting that this protein may also facilitate MCM3 nuclear localization. This gene is expressed in the brain or in neuronal tissue. An allelic variant encoding amino acid Lys at 915, instead of conserved Glu, has been identified in patients with mild intellectual disability. [provided by RefSeq, Jan 2014]