HELZ (NM_014877) Human Untagged Clone

CAT#: SC308909

HELZ (untagged)-Human helicase with zinc finger (HELZ)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: HELZ
• Synonyms: DHRC; DRHC; HUMORF5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308909 representing NM_014877.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGAAGACAGAAGAGCTGAAAAGTCATGTGAACAAGCATGTGAATCACTTAAGAGGCAGGACTATGAA
  ATGGCCCTCAAGCACTGCACAGAGGCCCTTCTTTCTCTTGGCCAGTACTCCATGGCAGACTTCACAGGG
  CCTTGTCCATTGGAAATAGAACGCATCAAAATCGAGAGTCTTCTCTACAGAATTGCCTCATTTTTGCAA
  CTGAAAAATTATGTGCAAGCTGATGAAGATTGTAGACATGTGCTGGGAGAAGGACTGGCCAAGGGAGAA
  GATGCCTTTCGGGCAGTGCTTTGCTGCATGCAGCTGAAAGGGAAGCTCCAACCTGTATCCACCATTCTT
  GCCAAGTCACTCACAGGAGAGTCCCTGAATGGGATGGTAACAAAGGATTTGACAAGACTAAAAACACTT
  CTCTCAGAAACAGAGACAGCAACTAGTAACGCCCTCTCTGGATATCACGTGGAAGACTTAGATGAGGGG
  TCTTGTAATGGTTGGCATTTCCGCCCACCACCTAGGGGAATCACAAGCAGCGAGGAATATACTTTGTGT
  AAAAGATTTTTAGAACAAGGAATCTGTAGGTATGGTGCCCAGTGTACTTCAGCACATTCCCAGGAAGAA
  CTAGCAGAATGGCAGAAAAGATATGCTTCACGGCTGATAAAATTGAAACAGCAAAATGAGAATAAACAG
  CTCTCAGGCAGTTACATGGAAACCTTGATAGAAAAGTGGATGAATTCATTGTCTCCTGAGAAAGTGCTT
  AGTGAATGTATAGAAGGAGTAAAGGTAGAGCACAATCCTGACCTGTCAGTTACTGTCAGCACCAAAAAA
  TCCCACCAGACATGGACCTTTGCTCTCACTTGTAAGCCTGCAAGAATGCTGTATCGTGTAGCATTGCTT
  TATGATGCTCATCGTCCTCATTTTAGTATCATTGCAATATCTGCCGGAGATAGTACTACCCAGGTATCA
  CAAGAAGTCCCAGAAAACTGTCAAGAATGGATAGGAGGAAAGATGGCCCAAAATGGATTAGATCATTAC
  GTGTATAAAGTCGGGATAGCATTTAACACAGAAATATTTGGAACTTTTCGCCAAACCATAGTTTTCGAC
  TTTGGATTGGAACCAGTACTCATGCAAAGAGTAATGATTGATGCAGCTTCTACAGAAGATCTCGAATAC
  CTGATGCATGCAAAACAGCAGCTAGTAACCACAGCTAAACGTTGGGATTCTTCCTCTAAGACTATTATA
  GATTTTGAACCTAATGAAACTACTGATTTGGAGAAGAGCCTTCTTATCAGATACCAAATTCCCCTCTCT
  GCTGACCAGCTATTTACTCAGTCCGTTTTAGACAAATCATTGACCAAGAGCAACTATCAGTCACGGTTA
  CATGACCTTCTTTATATTGAGGAGATAGCCCAGTATAAAGAAATCAGCAAGTTCAACCTTAAAGTGCAA
  TTGCAGATTCTGGCAAGCTTCATGCTCACTGGTGTTTCTGGAGGTGCAAAGTATGCTCAGAATGGACAA
  CTTTTTGGTCGCTTTAAGCTTACTGAAACACTTTCTGAAGATACTTTGGCTGGACGACTGGTGATGACC
  AAAGTCAATGCTGTTTATTTATTACCAGTCCCTAAACAGAAGTTAGTACAGACCCAGGGAACCAAAGAG
  AAGGTTTATGAAGCTACTATTGAAGAAAAAACAAAGGAATATATATTTTTAAGGCTATCTAGGGAATGC
  TGTGAAGAACTTAATCTTCGGCCTGACTGTGACACACAGGTTGAACTTCAGTTTCAATTAAATCGATTA
  CCCCTCTGTGAAATGCACTATGCACTAGACAGGATCAAGGACAATGGGGTTTTGTTTCCAGACATCAGT
  ATGACTCCCACCATACCATGGAGTCCTAACAGACAATGGGATGAACAGTTGGATCCTCGACTAAATGCA
  AAACAGAAAGAGGCTGTTCTGGCCATTACCACTCCACTTGCAATCCAGCTGCCGCCTGTGCTTATCATC
  GGACCCTATGGGACAGGCAAAACGTTCACTCTAGCTCAGGCTGTCAAACATATTCTGCAGCAACAGGAG
  ACTAGGATTCTCATTTGCACCCATTCTAATAGTGCTGCTGATCTCTACATAAAGGATTATTTACATCCA
  TATGTAGAAGCAGGCAATCCCCAGGCAAGACCTCTCAGGGTATATTTCAGAAATCGCTGGGTAAAGACT
  GTCCACCCAGTTGTGCATCAGTACTGTTTGATCTCAAGCGCACATTCCACCTTTCAGATGCCCCAGAAA
  GAAGATATTCTTAAACATCGAGTGGTGGTTGTTACCTTGAATACTTCCCAGTACCTCTGTCAGTTGGAC
  CTTGAACCTGGGTTTTTTACACACATTCTATTAGATGAAGCTGCCCAGGCCATGGAGTGTGAAACCATT
  ATGCCTCTAGCATTAGCAACTCAAAACACTCGGATTGTCTTGGCTGGTGATCACATGCAGCTCAGTCCT
  TTTGTTTACAGCGAGTTTGCCAGGGAGAGAAACCTTCACGTTTCATTACTTGACCGACTCTATGAGCAT
  TACCCTGCTGAGTTCCCATGTAGGATTCTCCTGTGTGAGAACTACCGCTCCCATGAAGCTATCATCAAT
  TATACCTCTGAGCTTTTCTATGAGGGCAAACTGATGGCCAGTGGGAAGCAGCCAGCACACAAAGATTTC
  TACCCACTAACTTTCTTTACAGCACGAGGAGAAGATGTACAAGAAAAAAATAGCACAGCTTTTTATAAT
  AATGCAGAGGTGTTTGAAGTGGTGGAACGTGTAGAAGAGTTAAGAAGGAAGTGGCCAGTAGCGTGGGGG
  AAGTTAGATGATGGCAGTATTGGTGTGGTGACTCCATATGCTGATCAAGTGTTTAGAATACGTGCTGAA
  CTTCGAAAAAAGAGATTATCTGATGTTAATGTAGAAAGGGTGCTAAATGTTCAAGGAAAGCAATTCAGA
  GTTTTGTTTCTTAGCACAGTACGTACAAGACATACTTGTAAACATAAACAGACACCAATTAAAAAGAAA
  GAGCAACTTCTGGAAGATTCCACAGAGGACTTAGATTATGGTTTTTTATCTAACTACAAGCTTCTCAAT
  ACTGCCATCACAAGAGCACAATCCCTGGTTGCTGTGGTGGGTGATCCCATTGCTCTGTGCTCTATTGGA
  AGATGCAGGAAATTTTGGGAACGGTTTATTGCCCTGTGTCATGAAAACAGTAGCCTACATGGAATCACT
  TTTGAACAGATCAAAGCCCAGTTAGAGGCTTTAGAACTAAAGAAGACATATGTGTTGAATCCGCTGGCA
  CCTGAATTTATCCCCCGGGCTCTAAGACTGCAGCATTCAGGAAGTACCAACAAACAGCAGCAATCACCA
  CCCAAGGGGAAAAGTCTTCATCATACCCAGAATGATCACTTCCAGAATGATGGAATTGTTCAGCCCAAT
  CCTTCTGTACTTATTGGCAATCCTATTAGAGCATATACTCCTCCACCCCCTCTTGGACCTCACCCAAAT
  TTGGGAAAATCTCCAAGCCCTGTTCAAAGAATAGATCCTCACACTGGGACAAGTATTCTTTATGTACCT
  GCTGTCTATGGAGGGAATGTAGTTATGTCGGTGCCTTTACCTGTACCATGGACAGGATACCAGGGTAGG
  TTTGCAGTTGATCCTCGAATTATTACACATCAGGCAGCAATGGCCTATAACATGAACCTATTACAGACA
  CATGGACGAGGATCTCCTATTCCTTATGGCCTTGGACATCACCCACCTGTCACCATAGGCCAGCCACAA
  AATCAGCATCAGGAGAAGGATCAACATGAGCAAAATCGAAATGGTAAAAGTGATACAAATAATTCCGGA
  CCTGAAATTAATAAGATTCGAACACCAGAGAAAAAGCCAACAGAACCAAAACAGGTTGATTTGGAATCA
  AATCCACAGAACAGAAGTCCTGAATCACGTCCTAGTGTTGTTTATCCCAGTACCAAATTTCCTCGCAAA
  GATAATCTCAACCCAAGACACATAAATCTTCCCCTTCCTGCTCCCCACGCACAGTATGCAATCCCTAAT
  CGCCACTTTCATCCCCTTCCCCAGCTACCAAGACCACCCTTTCCAATTCCACAGCAGCACACCTTGTTA
  AATCAGCAGCAGAATAATTTGCCTGAACAACCAAATCAGATACCACCTCAGCCAAATCAGGTAGTCCAG
  CAGCAAAGTCAGTTGAATCAGCAGCCTCAGCAGCCACCTCCTCAGCTTTCTCCTGCATATCAGGCGGGA
  CCCAACAATGCTTTTTTTAATAGTGCAGTTGCTCATCGGCCACAGTCTCCTCCTGCAGAAGCTGTAATT
  CCGGAGCAGCAGCCCCCTCCCATGCTGCAAGAAGGCCACAGTCCTCTGAGAGCCATTGCACAACCCGGC
  CCCATTCTTCCTTCACATCTGAATAGCTTCATTGATGAGAACCCCTCGGGATTACCTATAGGGGAGGCT
  TTAGATCGTATACATGGGAGTGTCGCTCTGGAAACATTAAGGCAGCAGCAGGCACGGTTCCAGCAGTGG
  AGCGAGCATCATGCCTTTCTCAGTCAGGGCAGCGTTCCATACCCACACCATCACCATCCTCACCTCCAG
  CATCTTCCTCAGCCGCCCCTGGGATTACATCAGCCGCCAGTGAGGGCAGACTGGAAGCTCACCAGCAGT
  GCCGAAGATGAAGTGGAGACCACATACTCAAGGTTTCAAGACTTAATCAGAGAACTGTCTCATCGTGAT
  CAAAGTGAAACACGGGAACTAGCTGAAATGCCACCACCTCAATCAAGACTTTTGCAATATAGACAAGTA
  CAGAGTAGAAGCCCACCAGCAGTCCCATCTCCCCCATCCAGTACAGACCACAGTAGCCACTTTTCTAAC
  TTTAATGATAACAGCAGAGACATTGAAGTAGCCAGCAACCCAGCATTTCCACAGCGCCTCCCACCCCAG
  ATATTCAACTCACCTTTCTCGTTGCCATCTGAACACCTTGCCCCTCCTCCCTTGAAATACCTGGCACCT
  GATGGAGCATGGACTTTTGCTAACTTGCAACAGAATCACCTAATGGGGCCAGGTTTTCCCTATGGCCTA
  CCTCCATTGCCTCACAGGCCACCGCAGAACCCTTTTGTACAAATACAGAATCATCAACATGCTATTGGT
  CAAGAGCCATTTCACCCATTGTCATCTCGAACAGTATCTTCTTCTTCGCTCCCTAGCTTAGAAGAGTAT
  GAGCCCAGAGGACCTGGTCGGCCCTTGTACCAAAGAAGAATCTCATCTAGCTCAGTTCAACCTTGTTCT
  GAAGAAGTAAGCACTCCTCAAGACAGTCTGGCTCAGTGTAAAGAGCTTCAGGACCACAGTAACCAATCT
  TCTTTCAACTTTTCATCCCCGGAGTCCTGGGTAAACACCACCTCATCTACTCCTTATCAGAACATTCCG
  TGCAATGGATCCAGCAGGACAGCTCAGCCCAGAGAGTTGATAGCGCCACCCAAGACTGTCAAACCCCCT
  GAGGATCAACTGAAGTCGGAGAACCTCGAGGTGTCCAGTTCCTTCAACTACAGTGTGCTGCAGCATCTT
  GGCCAGTTTCCACCCCTTATGCCTAACAAGCAGATCGCGGAGTCGGCCAATAGCAGTAGCCCCCAGAGC
  TCTGCGGGGGGCAAGCCCGCCATGTCCTATGCCAGCGCTCTGCGGGCCCCTCCAAAGCCCAGGCCCCCT
  CCTGAGCAGGCCAAGAAGAGTAGCGACCCTCTGTCTCTCTTCCAGGAACTGAGCCTAGGGAGCTCATCT
  GGCAGCAATGGCTTTTACTCATATTTTAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014877
• Insert Size: 5829 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014877.3
• RefSeq Size: 13847 bp
• RefSeq ORF: 5829 bp
• Locus ID: 9931
• UniProt ID: P42694
• Cytogenetics: 17q24.2
• Domains: zf-CCCH
• MW: 219 kDa
• Gene Summary: HELZ is a member of the superfamily I class of RNA helicases. RNA helicases alter the conformation of RNA by unwinding double-stranded regions, thereby altering the biologic activity of the RNA molecule and regulating access to other proteins (Wagner et al., 1999 [PubMed 10471385]).[supplied by OMIM, Mar 2008]