5HT3A receptor (HTR3A) (NM_213621) Human Untagged Clone

CAT#: SC308684

HTR3A (untagged)-Human 5-hydroxytryptamine (serotonin) receptor 3A (HTR3A), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: 5HT3A receptor
• Synonyms: 5-HT-3; 5-HT3A; 5-HT3R; 5HT3R; HTR3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_213621 edited
  GCAGTGGCCACGAGAGGCAGGCTGGCTGGGACATGAGGTTGGCAGAGGGCAGGCAAGCTG
  GCCCTTGGTGGGCCTCGTCCTGAGCACTCGGAGGCACTCCTATGCTTGGAAAGCTCGCTA
  TGCTGCTGTGGGTCCAGCAGGCGCTGCTCGCCTTGCTCCTCCCCACACTCCTGGCACAGG
  GAGAAGCCAGGAGGAGCCGAAACACCACCAGGCCCGCTCTGCTGAGGCTGTCGGATTACC
  TTTTGACCAACTACAGGAAGGGTGTGCGCCCCGTGAGGGACTGGAGGAAGCCAACCACCG
  TATCCATTGACGTCATTGTCTATGCCATCCTCAACGTGGATGAGAAGAATCAGGTGCTGA
  CCACCTACATCTGGTACCGGCAGTACTGGACTGATGAGTTTCTCCAGTGGAACCCTGAGG
  ACTTTGACAACATCACCAAGTTGTCCATCCCCACGGACAGCATCTGGGTCCCGGACATTC
  TCATCAATGAGTTCGTGGATGTGGGGAAGTCTCCAAATATCCCGTACGTGTATATTCGGC
  ATCAAGGCGAAGTTCAGAACTACAAGCCCCTTCAGGTGGTGACTGCCTGTAGCCTCGACA
  TCTACAACTTCCCCTTCGATGTCCAGAACTGCTCGCTGACCTTCACCAGTTGGCTGCACA
  CCATCCAGGACATCAACATCTCTTTGTGGCGCTTGCCAGAAAAGGTGAAATCCGACAGGA
  GTGTCTTCATGAACCAGGGAGAGTGGGAGTTGCTGGGGGTGCTGCCCTACTTTCGGGAGT
  TCAGCATGGAAAGCAGTAACTACTATGCAGAAATGAAGTTCTATGTGGTCATCCGCCGGC
  GGCCCCTCTTCTATGTGGTCAGCCTGCTACTGCCCAGCATCTTCCTCATGGTCATGGACA
  TCGTGGGCTTCTACCTGCCCCCCAACAGTGGCGAGAGGGTCTCTTTCAAGATTACACTCC
  TCCTGGGCTACTCGGTCTTCCTGATCATCGTTTCTGACACGCTGCCGGCCACTGCCATCG
  GCACTCCTCTCATTGGTAAGGCCCCTCCTGGCAGCAGAGCTCAGTCTGGTGAGAAACCCG
  CCCCCTCCCACCTCCTGCATGTGTCTCTTGCCTCTGCCCTGGGCTGCACAGGTGTCTACT
  TTGTGGTGTGCATGGCTCTGCTGGTGATAAGTTTGGCCGAGACCATCTTCATTGTGCGGC
  TGGTGCACAAGCAAGACCTGCAGCAGCCCGTGCCTGCTTGGCTGCGTCACCTGGTTCTGG
  AGAGAATCGCCTGGCTACTTTGCCTGAGGGAGCAGTCAACTTCCCAGAGGCCCCCAGCCA
  CCTCCCAAGCCACCAAGACTGATGACTGCTCAGCCATGGGAAACCACTGCAGCCACATGG
  GAGGACCCCAGGACTTCGAGAAGAGCCCGAGGGACAGATGTAGCCCTCCCCCACCACCTC
  GGGAGGCCTCGCTGGCGGTGTGTGGGCTGCTGCAGGAGCTGTCCTCCATCCGGCAATTCC
  TGGAAAAGCGGGATGAGATCCGAGAGGTGGCCCGAGACTGGCTGCGCGTGGGCTCCGTGC
  TGGACAAGCTGCTATTCCACATTTACCTGCTGGCGGTGCTGGCCTACAGCATCACCCTGG
  TTATGCTCTGGTCCATCTGGCAGTACGCTTGAGTGGGTACAGCCCAGTGGAGGAGGGGGT
  ACAGTCCTGGTTAGGTGGGGACAGAGGATTTCTGCTTAGGCCCCTCAGGACCCAGGGAAT
  GCCAGGGACATTTTCAAGACACAGACAAAGTCCCGTGCCCTGTTTCCAATGCCAATTCAT
  CTCAGCAATCACAAGCCAAGGTCTGAACCCTTCCACCAAAAACTGGGTGTTCAAGGCCCT
  TACACCCTTGTCCCACCCCCAGCAGCTCACCATGGCTTTAAAACATGCTCTCTTAGATCA
  GGAGAAACTCGGGCACTCCCTAAGTCCACTCTAGTTGTGGACTTTTCCCCATTGACCCTC
  ACCTGAATAAGGGACTTTGGAATTCTGCTTCTCTTTCACAACTTTGCTTTTAGGTTGAAG
  GCAAAACCAACTCTCTACTACACAGGCCTGATAACTCTGTACGAGGCTTCTCTAACCCCT
  AGTGTCTTTTTTTTCTTCACCTCACTTGTGGCAGCTTCCCTGAACACTCATCCCCCATCA
  GATGATGGGAGTGGGAAGAATAAAATGCAGTGAAACCCTAAAAAAAAAAAAAAAAAAAAA
  AAAA
  
• Restriction Sites: Please inquire
• ACCN: NM_213621
• Insert Size: 2200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_213621.1.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_213621.1, NP_998786.1
• RefSeq Size: 2343 bp
• RefSeq ORF: 1533 bp
• Locus ID: 3359
• UniProt ID: P46098
• Cytogenetics: 11q23.2
• Protein Families: Druggable Genome, Ion Channels: Cys-loop Receptors, Transmembrane
• Gene Summary: The product of this gene belongs to the ligand-gated ion channel receptor superfamily. This gene encodes subunit A of the type 3 receptor for 5-hydroxytryptamine (serotonin), a biogenic hormone that functions as a neurotransmitter, a hormone, and a mitogen. This receptor causes fast, depolarizing responses in neurons after activation. It appears that the heteromeric combination of A and B subunits is necessary to provide the full functional features of this receptor, since either subunit alone results in receptors with very low conductance and response amplitude. Alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1), also known as h5-HT3RL, represents the longest transcript, and encodes the longest isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. CCDS Note: The coding region has been updated to shorten the N-terminus to one that is more supported by conservation.