GRASP1 (GRIPAP1) (NM_207672) Human Untagged Clone

CAT#: SC308625

GRIPAP1 (untagged)-Human GRIP1 associated protein 1 (GRIPAP1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GRASP1
• Synonyms: GRASP-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308625 representing NM_207672.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGCAAGCTCTGTCTGAGGAGGAGTTTCAGCGGATGCAGGCTCAGCTCCTGGAACTCCGGACAAAC
  AACTACCAGCTTTCAGATGAACTACGCAAGAATGGTGTTGAACTCACCAGTCTTCGACAGAAGGTCGCC
  TACTTGGATAAGGAGTTCAGCAAAGCTCAGAAGGCACTGAGCAAGAGCAAGAAAGCTCAGGAAGTCGAG
  GTATTGCTGAGTGAAAATGAGATGCTGCAGGCAAAGCTGCACAGCCAGGAGGAGGACTTCCGTTTGCAG
  AACAGCACACTAATGGCCGAGTTCAGCAAGGAACGCTATGGGAAAGAAGCCGGGAAGTTCTCAGCTGTC
  AGTGAGGGCCAGGGGGATCCCCCAGGGGGCCCGGCCCCCACCGTCCTGGCCCCCATGCCGTTGGCAGAG
  GTGGAGCTGAAATGGGAAATGGAGAAAGAGGAGAAGAGATTGCTCTGGGAACAGCTGCAAGGCTTAGAG
  AGCTCAAAGCAGGCCGAAACATCCAGGCTGCAGGAGGAACTTGCTAAGCTCTCCGAGAAACTGAAAAAG
  AAACAAGAAAGTTTTTGCCGTCTGCAGACAGAAAAGGAGACTCTGTTTAATGACAGCAGGAACAAGATT
  GAGGAATTACAACAACGGAAGGAAGCTGATCACAAAGCCCAGTTGGCTCGAACCCAGAAGCTGCAGCAG
  GAACTTGAGGCTGCCAATCAGAGCTTGGCAGAGCTGAGAGATCAGCGGCAGGGGGAGCGCCTGGAACAT
  GCAGCAGCTTTGCGGGCCCTACAAGATCAGGTATCCATCCAGAGTGCAGATGCACAGGAACAAGTGGAA
  GGGCTTTTGGCTGAGAACAATGCCTTGAGGACTAGCCTGGCTGCCCTGGAGCAGATCCAAACAGCAAAG
  ACCCAAGAACTGAATATGCTCCGGGAACAGACCACTGGGCTGGCAGCTGAGTTGCAGCAGCAGCAGGCT
  GAGTACGAGGACCTTATGGGACAGAAAGATGACCTCAACTCCCAGCTCCAGGAGTCATTACGGGCCAAT
  AGTCGACTGCTGGAACAACTTCAAGAAATAGGGCAGGAGAAGGAGCAGTTGACCCAGGAATTACAGGAG
  GCTCGGAAGAGTGCGGAGAAGCGGAAGGCCATGCTGGATGAGCTAGCAATGGAAACGCTGCAAGAGAAG
  TCCCAGCACAAGGAAGAGCTGGGAGCAGTTCGTCTACGGCATGAGAAGGAGGTGCTGGGGGTGCGTGCC
  CGCTATGAGCGTGAGCTCCGAGAGCTGCATGAAGACAAGAAGCGTCAGGAGGAGGAGCTCCGTGGGCAG
  ATCCGGGAGGAGAAGGCCCGGACACGGGAGCTGGAGACTCTCCAGCAGACAGTGGAAGAACTTCAAGCT
  CAGGTACATTCCATGGATGGAGCCAAGGGCTGGTTTGAACGGCGCTTGAAGGAAGCCGAGGGCAAGGAG
  GAGGAGCTACAGGATGTACGGGATCAGCTCGAGCAGGCCCAGGAGGAGCGGGACTGCCACCTGAAGACC
  ATTAGCAGCCTGAAGCAGGAGGTGAAGGACACAGTGGATGGGCAGAGGATCCTGGAGAAGAAGGGCAGT
  GCTGCGCTCAAGGACCTCAAGCGGCAGCTGCATTTGGAGCGGAAACGGGCAGATAAGCTGCAGGAGCGA
  CTGCAGGACATCCTCACTAACAGCAAGAGCCGCTCAGGCCTTGAGGAGCTGGTTCTCTCAGAGATGAAC
  TCACCAAGCCGGACCCAGACAGGGGACAGCAGTAGCATCTCCTCCTTCAGCTACCGGGAGATCTTGCGG
  GAAAAGGAGAGCTCGGCTGTTCCAGCCAGGGTAAGGGGAAAGGAAGAACCTACAGCTCCAGCTTCTCTC
  AACCCAAAAATCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_207672
• Insert Size: 1878 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207672.1
• RefSeq Size: 3260 bp
• RefSeq ORF: 1878 bp
• Locus ID: 56850
• Cytogenetics: Xp11.23
• Protein Families: Druggable Genome
• MW: 71.9 kDa
• Gene Summary: This gene encodes a guanine nucleotide exchange factor for the Ras family of small G proteins (RasGEF). The encoded protein interacts in a complex with glutamate receptor interacting protein 1 (GRIP1) and plays a role in the regulation of AMPA receptor function. [provided by RefSeq, Aug 2013]
  Transcript Variant: This variant (2) lacks an alternate in-frame coding region segment and uses a different splice site in the 3' coding region, compared to variant 1. The reading frame is changed, such that the resulting protein (isoform 2) has a shorter and distinct C-terminus when compared to isoform 1.