PADI6 (NM_207421) Human Untagged Clone

CAT#: SC308532

PADI6 (untagged)-Human peptidyl arginine deiminase, type VI (PADI6)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PADI6
• Synonyms: hPADVI; PREMBL2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308532 representing NM_207421.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTCAGCGTGGAGGGCCGAGCCATGTCCTTCCAGAGTATCATCCACCTGTCCCTGGACAGCCCTGTC
  CATGCCGTTTGTGTGTTGGGCACAGAAATCTGCTTGGATCTCAGCGGGTGTGCCCCCCAGAAGTGCCAG
  TGCTTCACCATCCATGGCTCTGGGAGGGTCTTGATCGATGTGGCCAACACGGTGATTTCTGAGAAGGAG
  GACGCCACCATCTGGTGGCCCCTGTCTGATCCCACGTACGCCACAGTGAAGATGACATCGCCCAGCCCT
  TCCGTGGATGCGGATAAGGTCTCGGTCACATACTATGGGCCCAACGAGGATGCCCCCGTGGGCACAGCT
  GTGCTGTACCTCACTGGCATTGAGGTCTCTCTAGAGGTAGACATCTACCGCAATGGGCAAGTTGAGATG
  TCAAGTGACAAACAGGCTAAGAAAAAATGGATCTGGGGTCCCAGCGGTTGGGGTGCCATCCTGCTTGTG
  AATTGCAACCCTGCTGATGTGGGCCAGCAACTTGAGGACAAGAAAACCAAGAAAGTGATCTTTTCAGAG
  GAAATAACGAATCTGTCCCAGATGACTCTGAATGTCCAAGGCCCCAGCTGTATCTTAAAGAAATATCGG
  CTAGTCCTCCATACCTCCAAGGAAGAGTCGAAGAAGGCGAGAGTCTACTGGCCCCAAAAAGACAACTCC
  AGTACCTTTGAGTTGGTGCTGGGGCCCGACCAGCACGCCTATACCTTGGCCCTCCTCGGGAACCACTTG
  AAGGAGACTTTCTACGTTGAAGCTATAGCATTCCCATCTGCCGAATTCTCAGGCCTCATCTCCTACTCT
  GTGTCCCTGGTGGAGGAGTCTCAAGACCCGTCAATTCCAGAGACTGTGCTGTACAAAGACACGGTGGTG
  TTCCGGGTGGCTCCCTGTGTCTTCATTCCCTGTACCCAGGTGCCTCTGGAGGTTTACCTGTGCAGGGAG
  CTGCAGCTGCAGGGTTTTGTGGACACAGTGACGAAGCTGAGTGAGAAGAGCAACAGCCAGGTGGCATCT
  GTCTATGAGGACCCCAACCGCCTGGGCAGGTGGCTCCAGGATGAGATGGCCTTCTGCTACACCCAGGCT
  CCCCACAAGACAACGTCCTTGATCCTCGACACACCTCAGGCCGCCGATCTCGATGAGTTCCCCATGAAG
  TACTCACTGAGCCCTGGTATTGGCTACATGATCCAGGACACTGAGGACCATAAAGTGGCCAGCATGGAT
  TCCATTGGGAACCTGATGGTGTCCCCACCTGTCAAGGTCCAAGGGAAAGAGTACCCGCTGGGCAGAGTC
  CTCATTGGCAGCAGCTTTTACCCCAGCGCAGAGGGCCGGGCCATGAGTAAGACCCTCCGAGACTTCCTC
  TATGCCCAGCAGGTCCAAGCGCCGGTGGAGCTCTACTCAGATTGGCTAATGACTGGCCACGTGGATGAG
  TTCATGTGCTTCATCCCCACAGATGACAAGAATGAGGGCAAAAAGGGCTTCCTGCTGCTCCTGGCCAGC
  CCCAGTGCCTGCTATAAACTGTTCCGAGAGAAACAGAAGGAAGGCTATGGCGACGCTCTTCTGTTTGAT
  GAGCTTAGAGCAGATCAGCTCCTGTCTAATGGAAGGGAAGCCAAAACCATCGACCAACTTCTGGCTGAT
  GAAAGCCTGAAGAAGCAGAATGAATACGTGGAGAAGTGCATTCACCTGAACCGTGACATCCTGAAGACG
  GAGCTGGGCCTGGTGGAACAGGACATCATCGAGATTCCCCAGCTGTTCTGCTTGGAGAAGCTGACTAAC
  ATCCCCTCTGACCAGCAGCCCAAGAGGTCCTTTGCGAGGCCATACTTCCCTGACCTGTTGCGGATGATT
  GTGATGGGCAAGAACCTGGGGATCCCCAAGCCTTTTGGGCCCCAAATCAAGGGGACCTGCTGCCTGGAA
  GAAAAGATTTGCTGCTTGCTGGAGCCCCTGGGCTTCAAGTGCACCTTCATCAATGACTTTGACTGTTAC
  CTGACAGAGGTCGGAGACATCTGTGCCTGTGCCAACATCCGCCGGGTGCCCTTTGCCTTCAAATGGTGG
  AAGATGGTACCTTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_207421
• Insert Size: 2085 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207421.4
• RefSeq Size: 2396 bp
• RefSeq ORF: 2085 bp
• Locus ID: 353238
• UniProt ID: Q6TGC4
• Cytogenetics: 1p36.13
• MW: 77.7 kDa
• Gene Summary: This gene encodes a member of the peptidyl arginine deiminase family of enzymes, which catalyze the post-translational deimination of proteins by converting arginine residues into citrullines in the presence of calcium ions. The family members have distinct substrate specificities and tissue-specific expression patterns. This protein may play a role in cytoskeletal reorganization in the egg and in early embryo development. [provided by RefSeq, Sep 2012]