NPAS4 (NM_178864) Human Untagged Clone

CAT#: SC307237

NPAS4 (untagged)-Human neuronal PAS domain protein 4 (NPAS4)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NPAS4
• Synonyms: bHLHe79; Le-PAS; NXF; PASD10
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_178864 edited
  GGGGAAGCACGGAGGAGGAAGCCGCCGGTGCGTCGGGACGGGAGCGCAGGTGCTCGGGCA
  CCCGAGCTGGAGCTCCGCAGCCGCCGGTCATGTACCGCTCCACCAAGGGCGCCTCCAAGG
  CGCGCCGGGACCAGATCAACGCCGAGATCCGGAACCTCAAGGAGCTGCTGCCGCTGGCCG
  AAGCGGACAAGGTCCGGCTGTCCTACCTGCACATCATGAGCCTCGCCTGCATCTACACTC
  GCAAGGGCGTCTTCTTCGCTGGTGGCACTCCTCTGGCGGGCCCCACGGGGCTTCTCTCAG
  CTCAAGAGCTTGAGGACATCGTAGCGGCACTACCCGGCTTTCTGCTTGTGTTCACAGCCG
  AGGGGAAATTGCTCTACCTGTCTGAGAGTGTGAGCGAGCATCTGGGCCACTCCATGGTGG
  ACCTGGTTGCCCAGGGTGACAGCATCTACGACATCATTGACCCAGCTGACCACCTCACTG
  TGCGCCAGCAACTCACCCTGCCCTCTGCCCTGGACACTGATCGCCTCTTCCGCTGCCGCT
  TCAACACCTCCAAGTCCCTCAGGCGCCAGAGTGCAGGCAACAAACTCGTGCTTATTCGAG
  GCCGATTCCATGCTCACCCACCTGGAGCCTACTGGGCAGGAAATCCCGTGTTCACAGCTT
  TCTGTGCCCCTCTGGAGCCGAGACCCCGCCCAGGTCCTGGCCCTGGCCCTGGCCCTGCCT
  CGCTCTTCCTGGCCATGTTCCAGAGCCGCCATGCTAAAGACCTGGCTCTACTGGACATCT
  CCGAGAGTGTCCTAATCTACCTGGGCTTTGAGCGCAGTGAACTGCTTTGTAAATCATGGT
  ATGGACTGCTGCACCCCGAGGACCTGGCCCACGCTTCTGCTCAACACTACCGCCTGTTGG
  CTGAGAGTGGAGATATTCAGGCAGAGATGGTGGTGAGGCTACAGGCCAAGACTGGAGGCT
  GGGCATGGATTTACTGCCTGTTATACTCAGAAGGTCCAGAGGGACCCATTACTGCCAATA
  ACTACCCAATCAGTGACATGGAAGCCTGGAGCCTCCGCCAGCAGTTGAACTCTGAAGACA
  CCCAGGCAGCTTATGTCCTGGGCACTCCGACCATGCTGCCCTCATTCCCTGAAAACATTC
  TTTCCCAGGAAGAGTGCTCCAGCACTAACCCACTCTTCACCGCAGCACTGGGGGCTCCCA
  GAAGCACCAGCTTCCCCAGTGCTCCTGAACTGAGTGTTGTCTCTGCATCAGAAGAGCTTC
  CCCGACCCTCCAAAGAACTGGACTTCAGTTACCTGACATTCCCTTCTGGGCCTGAGCCTT
  CTCTCCAAGCAGAACTAAGCAAGGATCTTGTGTGCACTCCACCTTACACGCCCCATCAGC
  CAGGAGGCTGTGCCTTCCTCTTCAGCCTCCATGAGCCCTTCCAGACCCATTTGCCCACCC
  CATCCAGCACTCTTCAAGAACAGCTGACTCCAAGCACTGCGACCTTCTCTGATCAGTTGA
  CGCCCAGCAGTGCAACCTTCCCAGATCCACTAACTAGCCCACTGCAAGGCCAGTTGACTG
  AAACCTCGGTCAGAAGCTATGAAGACCAGTTGACTCCCTGCACCTCCACCTTCCCAGACC
  AGCTGCTTCCCAGCACAGCCACCTTCCCAGAGCCTCTGGGCAGCCCTGCCCATGAACAGC
  TGACTCCTCCCAGCACAGCATTCCAAGCACACCTGGACAGCCCCAGCCAAACCTTCCCAG
  AGCAACTGAGCCCCAACCCTACCAAGACTTACTTTGCCCAGGAGGGATGCAGTTTTCTCT
  ATGAGAAGTTGCCCCCAAGTCCTAGCAGCCCTGGTAATGGGGACTGCACGCTCTTGGCCC
  TAGCCCAGCTCCGGGGCCCCCTCTCTGTGGATGTCCCCCTGGTGCCCGAAGGCCTGCTCA
  CACCTGAGGCCTCTCCAGTCAAGCAGAGTTTCTTCCACTACTCTGAAAAGGAGCAGAATG
  AGATAGACCGTCTCATCCAGCAGATTAGCCAATTGGCTCAGGGCATGGACAGACCCTTCT
  CAGCTGAGGCTGGCACTGGCGGACTAGAGCCACTTGGAGGACTGGAGCCCCTGGACTCCA
  ACCTGTCCCTGTCAGGGGCAGGCCCCCCTGTGCTCAGCCTGGACCTGAAACCCTGGAAAT
  GCCAGGAGCTGGACTTCCTGGCTGACCCTGATAACATGTTCCTGGAAGAGACGCCCGTGG
  AAGACATCTTCATGGATCTCTCTACCCCAGATCCCAGTGAGGAATGGGGCTCAGGGGATC
  CTGAGGCAGAGGGCCCAGGAGGGGCCCCATCGCCTTGCAACAACCTGTCCCCAGAAGACC
  ACAGCTTCCTGGAGGACCTGGCCACATATGAAACCGCCTTTGAGACAGGTGTCTCAGCAT
  TCCCCTATGATGGGTTTACTGATGAGTTGCATCAACTCCAGAGCCAAGTTCAAGACAGCT
  TCCATGAAGATGGAAGTGGAGGGGAACCAACGTTTTGAATAAGTCTGTGACTTAACGTCG
  TCAAGTATGGCATATTGTCATCAAGACGTGGAGCCGCTCTCCAC
  
• Restriction Sites: Please inquire
• ACCN: NM_178864
• Insert Size: 2600 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_178864.2, NP_849195.1
• RefSeq Size: 3302 bp
• RefSeq ORF: 2409 bp
• Locus ID: 266743
• UniProt ID: Q8IUM7
• Cytogenetics: 11q13.2
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM, Mar 2008]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).