ZNF215 (NM_013250) Human Untagged Clone

CAT#: SC303991

ZNF215 (untagged)-Human zinc finger protein 215 (ZNF215)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ZNF215
• Synonyms: BAZ-2; BAZ2; ZKSCAN11; ZSCAN43
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303991 representing NM_013250.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCAGCCTCTGAGCAAGTTGATGGCTATCTCAAAACCTCGAAACCTGTCTCTACGTGAACAAAGAGAG
  GTTCTGAGAGCAGATATGTCTTGGCAGCAGGAAACCAACCCCGTCGTGGAGACACATGACTCTGAGGCA
  TCTCGTCAAAAGTTCAGACATTTCCAGTATTTGAAAGTGTCTGGGCCCCATGAAGCCCTGAGCCAACTC
  TGGGAGCTCTGTCTTCAATGGCTGAGACCAGAGATTCATACAAAGAAGCAGATTATAGAACTGTTGGTG
  CTGGAACAATTCCTGGCAATCCTGCCTGAAGAAGTCAGGACTTGGGTGAATTTACAACATCCAAACAAC
  AGTAAAGATATGGTGACCCTCATAGAAGATGTGATTGAAATGCTTGAAGATGAAGATATGCCCTGCAAG
  GACTCTGCCCTGCAGATGGGGAGCATCAAGGAGAAAATGAAAGCTGGCTCACGAACAGGCAAACCACAG
  GAACCAGTGACATTCAAAGATGTGGTTGTGGAATTCAGCAAGGAAGAGTGGGGGCAACTGGACTCTGCT
  GTAAAGAACCTGTACAGGAATGTGATGCTGGAAAACTTTAGGAACCTGAATTCATTGCGTAAAGCACAT
  CTACTTTCCAAACCATTTGAGAGCCTTAAGTTGGAGAGTAAGAAAAAAAGATGGATAATGGAGAAAGAA
  ATACCAAGGAAGACTATTTTTGACATGAAGAGTATTTCTGGAGAAGAATCATCCCATGGAGTGATTATG
  ACAAGGCTTACCGAAAGTGGACACCCTTCTTCAGATGCCTGGAAAGGTGAGAATTGGTTATATAGGAAC
  CAGAAAAAATGGGACATAAATTTGCCACAAGAGGCTTTCATTCCTGAGACAATTTACACTGAGGAGGAA
  GATTTTGAATGTAGTGAAAATAAGAAAAGCTTTGATATTAATTCAGTTAGCTCAATTTGTGCTATACAA
  GTGGGAATTCCTTCAAGAAAGGGGTCTCCAAAATGTGATAAGTTTAAAACTTACTTCAAATTTAATTTA
  GACTCAGTAGGTAAGCAACATTCAGAATATGAATATGGGAATGACTTGAGTTTGAGTACAGATATTCGA
  CACCAAAAAAGTCATACTACAATGAATTCCTATGAATGTTATCAATGTGGGAAAGCCTTCTGCCGAAGT
  TCATCCCTTATTCGACATCAGATCATTCACACAGGAGAGAAACCCTATAAATGCAGTGAATGTGGGAGA
  TTCTTCAACCGACGTACAAACCTTACTAAGCATCAAAAACTTCATGCTGAAGCAAAGGCCTGCACAAGC
  AATAAATGTGGAAAGGCCTTCAGTAAAAGTGAAGACAGTAATAATCCAACACTCCATTTTGGAAACAAT
  TTCTATCAATGTGTTAACTGTGGAAAATCCTTCAACCGGAGCTCCTCTCTTATTCGACACCAAATGATT
  CACACGGGAGAGAAACCATTCAAATGTAAGGAATGTAGTAAAGCCTTCAACAGGAGTTCAAACCTTGTT
  AAACATCAAAAACTGCATACTCGAGATAAGTCCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_013250
• Insert Size: 1554 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_013250.2
• RefSeq Size: 3658 bp
• RefSeq ORF: 1554 bp
• Locus ID: 7762
• UniProt ID: Q9UL58
• Cytogenetics: 11p15.4
• Protein Families: Transcription Factors
• MW: 60 kDa
• Gene Summary: This gene is imprinted in a tissue-specific manner with preferential expression in the testis, and encodes a zinc finger protein that belongs to a family of zinc finger transcription factors. The encoded protein contains an N-terminal SRE-ZBP, Ctfin51, AW-1, and Number 18 (SCAN) domain, a kruppel-associated box A (KRABA) domain, and four C-terminal zinc finger domains. This gene is located within one of three regions on chromosome 11p15 associated with Beckwith-Wiedemann syndrome, called Beckwith-Wiedemann syndrome chromosome region-2 (BWSCR2), and is thought to play a role in the etiology of this disease. [provided by RefSeq, Aug 2017]