ITGB1BP2 (NM_012278) Human Untagged Clone

CAT#: SC303950

ITGB1BP2 (untagged)-Human integrin beta 1 binding protein (melusin) 2 (ITGB1BP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ITGB1BP2
• Synonyms: CHORDC3; ITGB1BP; MELUSIN; MSTP015
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC303950 representing NM_012278.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTCTACTCTGTCGTAACAAAGGCTGTGGGCAGCACTTTGACCCTAATACCAACCTTCCTGATTCC
  TGTTGCCATCACCCTGGGGTCCCAATCTTCCATGATGCACTTAAGGGTTGGTCCTGCTGCCGAAAGCGA
  ACTGTAGATTTCTCTGAGTTCTTAAACATCAAGGGCTGTACTATGGGACCACACTGTGCTGAGAAGCTT
  CCTGAGGCCCCTCAACCTGAAGGCCCTGCTACAAGCAGTTCACTTCAGGAGCAAAAACCTCTGAATGTG
  ATTCCAAAGTCAGCAGAGACCTTGCGCCGGGAGAGGCCCAAGTCAGAGTTGCCTCTGAAGCTGCTGCCG
  CTAAATATATCCCAAGCCCTGGAAATGGCATTGGAACAGAAGGAATTAGACCAGGAACCTGGGGCAGGA
  CTTGACAGTCTGATCCGGACTGGTTCCAGCTGCCAGAACCCAGGATGTGATGCTGTTTACCAAGGCCCT
  GAGAGTGATGCTACTCCATGTACCTACCACCCAGGAGCACCCCGATTCCATGAGGGGATGAAGTCTTGG
  AGCTGTTGTGGCATCCAGACCCTGGATTTTGGGGCATTCTTGGCACAACCAGGGTGCAGAGTCGGTAGA
  CATGACTGGGGGAAGCAGCTCCCAGCATCTTGCCGCCATGATTGGCACCAGACAGATTCCTTAGTAGTG
  GTGACTGTATATGGCCAGATTCCACTTCCTGCGTTTAACTGGGTGAAGGCCAGTCAAACTGAGCTTCAT
  GTCCACATTGTCTTTGATGGTAACCGTGTGTTCCAAGCACAGATGAAGCTCTGGGGGGTCATAAACGTG
  GAGCAGAGCTCTGTCTTCTTGATGCCATCTCGGGTTGAAATCTCCCTGGTCAAGGCTGACCCAGGATCC
  TGGGCCCAGCTGGAGCACCCTGATGCACTAGCTAAGAAGGCTAGGGCAGGGGTTGTGTTAGAGATGGAT
  GAGGAAGAATCTGACGATTCAGATGATGATCTGAGCTGGACAGAGGAGGAGGAAGAGGAGGAAGCAATG
  GGGGAATAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_012278
• Insert Size: 1044 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_012278.2
• RefSeq Size: 1292 bp
• RefSeq ORF: 1044 bp
• Locus ID: 26548
• UniProt ID: Q9UKP3
• Cytogenetics: Xq13.1
• MW: 38.4 kDa
• Gene Summary: This gene encodes a protein with two cysteine and histidine-rich (CHORD) domains, PXXP motifs, YXXI/P motifs, putative SH2 and SH3 domain binding motifs, and an acidic region at the C-terminus that can bind calcium. Two hybrid analysis showed that this protein interacts with the cytoplasmic domain of the beta 1 integrin subunit and is thought to act as a chaperone protein. Studies in the mouse ortholog of this gene indicate that absence of this gene in mouse results in failed cardiac hypertrophy in response to mechanical stress. Alternative splicing results in multiple transcript variants encoding different isoforms, including an isoform that lacks several domains, including one of the CHORD domains. [provided by RefSeq, May 2017]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1).