SFI1 (NM_001007467) Human Untagged Clone

CAT#: SC301214

SFI1 (untagged)-Human Sfi1 homolog, spindle assembly associated (yeast) (SFI1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SFI1
• Synonyms: hSfi1p; PISD; PPP1R139
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC301214 representing NM_001007467.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAAGAATCTGCTCACTGAGAAGTGTATATCAAGCCACAATTTCCATCAAAAAGTGATTAAGCAGAGA
  ATGGAGAAGAAGGTTGATTCCAGGTATTTTAAGGATGGTGCAGTTAAGAAACCTTATTCTGCAAAGACA
  CTGTCCAACAAGAAGTCTTCTGCATCCTTTGGGATCCGGAGGGAGTTACCTAGTACCAGTCATCTAGTG
  CAGTATCGTGGCACACATACTTGTACCCGACAGGGCCGGTTAAGAGAACTGCGCATCAGATGCGTGGCC
  AGAAAGTTCTTATATTTATGGATTCGAATGACTTTTGGAAGAGTATTTCCCTCTAAAGCCAGATTTTAC
  TATGAGCAGCGATTACTACGGAAGGTCTTCGAAGAATGGAAAGAGGAGTGGTGGGTTTTCCAGCACGAG
  TGGAAACTCTGTGTTCGAGCTGACTGTCACTACAGGTATTACCTGTACAACCTGATGTTCCAGACGTGG
  AAGACCTATGTGCGTCAGCAGCAGGAGATGAGGAACAAGTACATTAGAGCCGAGGTTCATGATGCAAAG
  CAAAAGATGCGACAGGCCTGGAAGTCCTGGTTGATCTACGTGGTTGTTCGTAGGACCAAACTTCAGATG
  CAGACTACAGCTCTGGAGTTTAGGCAACGGATTATCTTACGGGTGTGGTGGAGCACGTGGAGGCAGCGA
  CTAGGACAGGTCCGTGTGAGCCGTGCCCTCCATGCCTCTGCTTTGAAGCACAGGGCCCTGAGCCTCCAG
  GTGCAGGCTTGGTCACAGTGGCGGGAACAGCTCCTGTATGTCCAGAAGGAGAAACAAAAGGTTGTCTCT
  GCAGTGAAACATCATCAGCACTGGCAAAAACGGAGATTTCTAAAGGCCTGGCTTGAATACCTGCAAGTC
  CGCAGAGTGAAGAGACAGCAGAATGAGATGGCTGAGCGATTCCATCATGTCACTGTGCTCCAGATATAC
  TTCTGTGACTGGCAGCAGGCCTGGGAGCGGAGGGAGAGCTTGTACGCTCACCATGCCCAGGTGGAGAAA
  CTGGCCAGGAAGATGGCCCTGCGGCGCGCCTTTACTCACTGGAAACACTACATGCTGCTGTGTGCAGAA
  GAAGCTGCCCAGTTTGAGATGGCAGAAGAGCACCACAGGCACAGCCAGCTGTATTTTTGCTTTAGAGCC
  CTAAAAGACAATGTGACCCACGCTCATCTCCAGCAAATAAGAAGGAATCTTGCTCACCAGCAGCATGGT
  GTCACGCTGCTGCACAGGTTCTGGAACCTCTGGCGGTCTCAGATTGAGCAGAAAAAGGAAAGAGAGCTG
  CTCCCCTTACTGCATGCTGCCTGGGACCACTACAGAATAGCACTGCTGTGCAAATGTATCGAATTGTGG
  CTACAGTATACTCAGAAGAGGCGGTACAAGCAGCTGCTACAGGCCAGAGCGGATGGTCATTTCCAGCAG
  AGAGCCCTGCCTGCTGCCTTCCACACATGGAACAGACTCTGGCGATGGCGCCACCAGGAAAATGTCCTC
  AGTGCAAGAGCAACACGTTTCCACAGGGAGACATTAGAGAAGCAAGTATTTTCTCTCTGGAGGCAGAAG
  ATGTTTCAGCATCGAGAAAACCGCCTGGCAGAGAGAATGGCCATCCTTCACGCAGAGCGACAGCTTCTG
  TATAGGTCTTGGTTCATGTGGCACCAGCAGGCAGCAGCACGTCACCAGGAGCAGGAGTGGCAAACAGTG
  GCCTGTGCCCACCACCGCCACGGGCGGCTCAAGAAAGCTTTCTGCCTCTGGAGGGAAAGTGCCCAAGGG
  CTCAGAACAGAGAGGACGGGCAGGGTGCGGGCAGCAGAATTCCACATGGCCCAGCTCCTGCGTTGGGCC
  TGGAGCCAGTGGAGGGAGTGCCTGGCCCTGCGGGGAGCGGAGCGGCAGAAGCTGATGCGAGCAGACCTG
  CACCACCAGCACAGCGTGCTGCACAGGGCGCTGCAGGCATGGGTGACTTACCAGGGCAGGGTGCGAAGC
  ATCCTCCGGGAGGTGGCAGCCAGGGAGAGCCAGCACAACAGGCAGCTGCTGCGCGGGGCATTACGTCGC
  TGGAAAGAGAACACCATGGCCCGAGTGGATGAAGCCAAAAAAACCTTTCAAGCAAGTACTCATTACAGA
  AGGACCATATGTTCCAAGGTCCTGGTCCAGTGGCGGGAAGCTGTGTCAGTGCAGATGTATTACCGACAG
  CAGGAGGACTGTGCCATCTGGGAGGCCCAGAAGGTGCTGGACAGGGGCTGTCTGCGGACCTGGTTTCAG
  CGCTGGTGGGACTGCAGCCGGAGGTCAGCCCAGCAGAGACTGCAGCTGGAGAGGGCAGTGCAACACCAC
  CACCGGCAGCTGCTGCTGGAGGGGCTGGCCCGGTGGAAGACGCACCATCTGCAGTGTGTCAGGAAGAGG
  CTCCTGCACAGGCAGAGCACCCAACTGCTGGCACAGAGACTCAGCCGGACCTGCTTCCGCCAGTGGAGA
  CAACAGCTGGCAGCCAGGAGGCAGGAGCAGCGGGCGACAGTGCGGGCCCTGTGGTTCTGGGCCTTCTCG
  CTGCAGGCAAAGGTGTGGGCCACGTGGCTGGCCTTTGTACTGGAAAGGAGGAGAAAGAAGGCGCGGCTG
  CAGTGGGCGCTCCAGGCCTACCAGGGGCAGCTCCTCCAGGAGGGTGCCACGCGGCTCCTGCGCTTTGCA
  GCCAGCATGAAGGCCTCCCGGCAGCAGCTGCAGGCCCAGCAGCAGGTCCAGGCGGCTCACAGTCTCCAT
  CGTGCCGTCCGCCGCTGTGCCACGCTCTGGAAACAGAAAGTGCTGGGCCGGGGCGGGAAGCCTCAGCCC
  CTGGCAGCCATCGCACCCAGCAGGAAAGTGACGTTTGAGGGTCCCCTTCTCAACCGCATTGCTGCTGGG
  GCTGGGGATGGCACCCTTGAGACCAAGAGGCCACAGGCTTCTCGGCCTCTGGGAGCTCTGGGCCGCCTG
  GCTGCTGAGGAGCCCCACGCCCTGGAGCTCAACACTGCCCACTCAGCGAGGAAGCAGCCGCGACGCCCA
  CACTTCCTGTTGGAGCCTGCGCAGAGCCAGAGGCCTCAGAAGCCACAGGAACATGGCCTAGGCATGGCT
  CAGCCAGCAGCCCCCTCCCTGACGCGGCCCTTCCTGGCAGAGGCCCCGACAGCACTGGTCCCACACAGC
  CCCCTGCCTGGGGCCCTGTCAAGCGCCCCTGGCCCGAAGCAGCCCCCGACGGCAAGCACAGGCCCGGAG
  CTGCTGCTGCTGCCTCTTTCCTCCTTCATGCCCTGCGGGGCGGCTGCACCAGCCAGGGTGTCAGCACAG
  CGGGCTACTCCTAGGGATAAGCCCCCGGTCCCCTCATCCCTGGCCAGTGTCCCTGACCCCCATCTACTC
  CTTCCTGGGGACTTCTCAGCCACCAGGGCTGGGCCTGGACTTTCAACTGCAGGCAGCCTGGACCTTGAG
  GCTGAACTTGAGGAGATCCAGCAGCAACTACTGCACTACCAGACCACCAAGCAGAACCTCTGGTCCTGT
  CGGCGGCAAGCGAGCAGCCTGCGCAGGTGGCTGGAGCTGAACAGAGAGGAGCCGGGGCCTGAGGACCAG
  GAAGTAGAGCAGCAGGTGCAGAAAGAGCTGGAACAGGTGGAAATGCAGATCCAGCTGCTGGCAGAGGAG
  CTCCAGGCTCAGCGCCAGCCCATTGGCGCCTGCGTTGCCCGCATCCAGGCCCTGCGGCAGGCCCTGTGC
  TAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001007467
• Insert Size: 3729 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007467.2
• RefSeq Size: 4391 bp
• RefSeq ORF: 3729 bp
• Locus ID: 9814
• UniProt ID: A8K8P3
• Cytogenetics: 22q12.2
• MW: 147.7 kDa
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).