SFI1 (NM_001007467) Human Tagged ORF Clone

CAT#: RC216567

SFI1 (Myc-DDK-tagged)-Human Sfi1 homolog, spindle assembly associated (yeast) (SFI1), transcript variant 1

Product Images

Circular map for RC216567

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SFI1
• Synonyms: hSfi1p; PISD; PPP1R139
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC216567 representing NM_001007467
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGAATCTGCTCACTGAGAAGTGTATATCAAGCCACAATTTCCATCAAAAAGTGATTAAGCAGAGAA
  TGGAGAAGAAGGTTGATTCCAGGTATTTTAAGGATGGTGCAGTTAAGAAACCTTATTCTGCAAAGACACT
  GTCCAACAAGAAGTCTTCTGCATCCTTTGGGATCCGGAGGGAGTTACCTAGTACCAGTCATCTAGTGCAG
  TATCGTGGCACACATACTTGTACCCGACAGGGCCGGTTAAGAGAACTGCGCATCAGATGCGTGGCCAGAA
  AGTTCTTATATTTATGGATTCGAATGACTTTTGGAAGAGTATTTCCCTCTAAAGCCAGATTTTACTATGA
  GCAGCGATTACTACGGAAGGTCTTCGAAGAATGGAAAGAGGAGTGGTGGGTTTTCCAGCACGAGTGGAAA
  CTCTGTGTTCGAGCTGACTGTCACTACAGGTATTACCTGTACAACCTGATGTTCCAGACGTGGAAGACCT
  ATGTGCGTCAGCAGCAGGAGATGAGGAACAAGTACATTAGAGCCGAGGTTCATGATGCAAAGCAAAAGAT
  GCGACAGGCCTGGAAGTCCTGGTTGATCTACGTGGTTGTTCGTAGGACCAAACTTCAGATGCAGACTACA
  GCTCTGGAGTTTAGGCAACGGATTATCTTACGGGTGTGGTGGAGCACGTGGAGGCAGCGACTAGGACAGG
  TCCGTGTGAGCCGTGCCCTCCATGCCTCTGCTTTGAAGCACAGGGCCCTGAGCCTCCAGGTGCAGGCTTG
  GTCACAGTGGCGGGAACAGCTCCTGTATGTCCAGAAGGAGAAACAAAAGGTTGTCTCTGCAGTGAAACAT
  CATCAGCACTGGCAAAAACGGAGATTTCTAAAGGCCTGGCTTGAATACCTGCAAGTCCGCAGAGTGAAGA
  GACAGCAGAATGAGATGGCTGAGCGATTCCATCATGTCACTGTGCTCCAGATATACTTCTGTGACTGGCA
  GCAGGCCTGGGAGCGGAGGGAGAGCTTGTACGCTCACCATGCCCAGGTGGAGAAACTGGCCAGGAAGATG
  GCCCTGCGGCGCGCCTTTACTCACTGGAAACACTACATGCTGCTGTGTGCAGAAGAAGCTGCCCAGTTTG
  AGATGGCAGAAGAGCACCACAGGCACAGCCAGCTGTATTTTTGCTTTAGAGCCCTAAAAGACAATGTGAC
  CCACGCTCATCTCCAGCAAATAAGAAGGAATCTTGCTCACCAGCAGCATGGTGTCACGCTGCTGCACAGG
  TTCTGGAACCTCTGGCGGTCTCAGATTGAGCAGAAAAAGGAAAGAGAGCTGCTCCCCTTACTGCATGCTG
  CCTGGGACCACTACAGAATAGCACTGCTGTGCAAATGTATCGAATTGTGGCTACAGTATACTCAGAAGAG
  GCGGTACAAGCAGCTGCTACAGGCCAGAGCGGATGGTCATTTCCAGCAGAGAGCCCTGCCTGCTGCCTTC
  CACACATGGAACAGACTCTGGCGATGGCGCCACCAGGAAAATGTCCTCAGTGCAAGAGCAACACGTTTCC
  ACAGGGAGACATTAGAGAAGCAAGTATTTTCTCTCTGGAGGCAGAAGATGTTTCAGCATCGAGAAAACCG
  CCTGGCAGAGAGAATGGCCATCCTTCACGCAGAGCGACAGCTTCTGTATAGGTCTTGGTTCATGTGGCAC
  CAGCAGGCAGCAGCACGTCACCAGGAGCAGGAGTGGCAAACAGTGGCCTGTGCCCACCACCGCCACGGGC
  GGCTCAAGAAAGCTTTCTGCCTCTGGAGGGAAAGTGCCCAAGGGCTCAGAACAGAGAGGACGGGCAGGGT
  GCGGGCAGCAGAATTCCACATGGCCCAGCTCCTGCGTTGGGCCTGGAGCCAGTGGAGGGAGTGCCTGGCC
  CTGCGGGGAGCGGAGCGGCAGAAGCTGATGCGAGCAGACCTGCACCACCAGCACAGCGTGCTGCACAGGG
  CGCTGCAGGCATGGGTGACTTACCAGGGCAGGGTGCGAAGCATCCTCCGGGAGGTGGCAGCCAGGGAGAG
  CCAGCACAACAGGCAGCTGCTGCGCGGGGCATTACGTCGCTGGAAAGAGAACACCATGGCCCGAGTGGAT
  GAAGCCAAAAAAACCTTTCAAGCAAGTACTCATTACAGAAGGACCATATGTTCCAAGGTCCTGGTCCAGT
  GGCGGGAAGCTGTGTCAGTGCAGATGTATTACCGACAGCAGGAGGACTGTGCCATCTGGGAGGCCCAGAA
  GGTGCTGGACAGGGGCTGTCTGCGGACCTGGTTTCAGCGCTGGTGGGACTGCAGCCGGAGGTCAGCCCAG
  CAGAGACTGCAGCTGGAGAGGGCAGTGCAACACCACCACCGGCAGCTGCTGCTGGAGGGGCTGGCCCGGT
  GGAAGACGCACCATCTGCAGTGTGTCAGGAAGAGGCTCCTGCACAGGCAGAGCACCCAACTGCTGGCACA
  GAGACTCAGCCGGACCTGCTTCCGCCAGTGGAGACAACAGCTGGCAGCCAGGAGGCAGGAGCAGCGGGCG
  ACAGTGCGGGCCCTGTGGTTCTGGGCCTTCTCGCTGCAGGCAAAGGTGTGGGCCACGTGGCTGGCCTTTG
  TACTGGAAAGGAGGAGAAAGAAGGCGCGGCTGCAGTGGGCGCTCCAGGCCTACCAGGGGCAGCTCCTCCA
  GGAGGGTGCCACGCGGCTCCTGCGCTTTGCAGCCAGCATGAAGGCCTCCCGGCAGCAGCTGCAGGCCCAG
  CAGCAGGTCCAGGCGGCTCACAGTCTCCATCGTGCCGTCCGCCGCTGTGCCACGCTCTGGAAACAGAAAG
  TGCTGGGCCGGGGCGGGAAGCCTCAGCCCCTGGCAGCCATCGCACCCAGCAGGAAAGTGACGTTTGAGGG
  TCCCCTTCTCAACCGCATTGCTGCTGGGGCTGGGGATGGCACCCTTGAGACCAAGAGGCCACAGGCTTCT
  CGGCCTCTGGGAGCTCTGGGCCGCCTGGCTGCTGAGGAGCCCCACGCCCTGGAGCTCAACACTGCCCACT
  CAGCGAGGAAGCAGCCGCGACGCCCACACTTCCTGTTGGAGCCTGCGCAGAGCCAGAGGCCTCAGAAGCC
  ACAGGAACATGGCCTAGGCATGGCTCAGCCAGCAGCCCCCTCCCTGACGCGGCCCTTCCTGGCAGAGGCC
  CCGACAGCACTGGTCCCACACAGCCCCCTGCCTGGGGCCCTGTCAAGCGCCCCTGGCCCGAAGCAGCCCC
  CGACGGCAAGCACAGGCCCGGAGCTGCTGCTGCTGCCTCTTTCCTCCTTCATGCCCTGCGGGGCGGCTGC
  ACCAGCCAGGGTGTCAGCACAGCGGGCTACTCCTAGGGATAAGCCCCCGGTCCCCTCATCCCTGGCCAGT
  GTCCCTGACCCCCATCTACTCCTTCCTGGGGACTTCTCAGCCACCAGGGCTGGGCCTGGACTTTCAACTG
  CAGGCAGCCTGGACCTTGAGGCTGAACTTGAGGAGATCCAGCAGCAACTACTGCACTACCAGACCACCAA
  GCAGAACCTCTGGTCCTGTCGGCGGCAAGCGAGCAGCCTGCGCAGGTGGCTGGAGCTGAACAGAGAGGAG
  CCGGGGCCTGAGGACCAGGAAGTAGAGCAGCAGGTGCAGAAAGAGCTGGAACAGGTGGAAATGCAGATCC
  AGCTGCTGGCAGAGGAGCTCCAGGCTCAGCGCCAGCCCATTGGCGCCTGCGTTGCCCGCATCCAGGCCCT
  GCGGCAGGCCCTGTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC216567 representing NM_001007467
  Red=Cloning site Green=Tags(s)
  
  MKNLLTEKCISSHNFHQKVIKQRMEKKVDSRYFKDGAVKKPYSAKTLSNKKSSASFGIRRELPSTSHLVQ
  YRGTHTCTRQGRLRELRIRCVARKFLYLWIRMTFGRVFPSKARFYYEQRLLRKVFEEWKEEWWVFQHEWK
  LCVRADCHYRYYLYNLMFQTWKTYVRQQQEMRNKYIRAEVHDAKQKMRQAWKSWLIYVVVRRTKLQMQTT
  ALEFRQRIILRVWWSTWRQRLGQVRVSRALHASALKHRALSLQVQAWSQWREQLLYVQKEKQKVVSAVKH
  HQHWQKRRFLKAWLEYLQVRRVKRQQNEMAERFHHVTVLQIYFCDWQQAWERRESLYAHHAQVEKLARKM
  ALRRAFTHWKHYMLLCAEEAAQFEMAEEHHRHSQLYFCFRALKDNVTHAHLQQIRRNLAHQQHGVTLLHR
  FWNLWRSQIEQKKERELLPLLHAAWDHYRIALLCKCIELWLQYTQKRRYKQLLQARADGHFQQRALPAAF
  HTWNRLWRWRHQENVLSARATRFHRETLEKQVFSLWRQKMFQHRENRLAERMAILHAERQLLYRSWFMWH
  QQAAARHQEQEWQTVACAHHRHGRLKKAFCLWRESAQGLRTERTGRVRAAEFHMAQLLRWAWSQWRECLA
  LRGAERQKLMRADLHHQHSVLHRALQAWVTYQGRVRSILREVAARESQHNRQLLRGALRRWKENTMARVD
  EAKKTFQASTHYRRTICSKVLVQWREAVSVQMYYRQQEDCAIWEAQKVLDRGCLRTWFQRWWDCSRRSAQ
  QRLQLERAVQHHHRQLLLEGLARWKTHHLQCVRKRLLHRQSTQLLAQRLSRTCFRQWRQQLAARRQEQRA
  TVRALWFWAFSLQAKVWATWLAFVLERRRKKARLQWALQAYQGQLLQEGATRLLRFAASMKASRQQLQAQ
  QQVQAAHSLHRAVRRCATLWKQKVLGRGGKPQPLAAIAPSRKVTFEGPLLNRIAAGAGDGTLETKRPQAS
  RPLGALGRLAAEEPHALELNTAHSARKQPRRPHFLLEPAQSQRPQKPQEHGLGMAQPAAPSLTRPFLAEA
  PTALVPHSPLPGALSSAPGPKQPPTASTGPELLLLPLSSFMPCGAAAPARVSAQRATPRDKPPVPSSLAS
  VPDPHLLLPGDFSATRAGPGLSTAGSLDLEAELEEIQQQLLHYQTTKQNLWSCRRQASSLRRWLELNREE
  PGPEDQEVEQQVQKELEQVEMQIQLLAEELQAQRQPIGACVARIQALRQALC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001007467
• ORF Size: 3726 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001007467.3
• RefSeq Size: 4302 bp
• RefSeq ORF: 3729 bp
• Locus ID: 9814
• UniProt ID: A8K8P3
• Cytogenetics: 22q12.2
• MW: 147.5 kDa
• Gene Summary: Plays a role in the dynamic structure of centrosome-associated contractile fibers via its interaction with CETN2.[UniProtKB/Swiss-Prot Function]