PIM3 (NM_001001852) Human Untagged Clone

CAT#: SC300319

PIM3 (untagged)-Human pim-3 oncogene (PIM3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PIM3
• Synonyms: pim-3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001001852 edited
  ATGCTGCTCTCCAAGTTCGGCTCCCTGGCGCACCTCTGCGGGCCCGGCGGCGTGGACCAC
  CTCCCGGTGAAGATCCTGCAGCCAGCCAAGGCGGACAAGGAGAGCTTCGAGAAGGCGTAC
  CAGGTGGGCGCCGTGCTGGGTAGCGGCGGCTTCGGCACGGTCTACGCGGGTAGCCGCATC
  GCCGACGGGCTCCCGGTGGCTGTGAAGCACGTGGTGAAGGAGCGGGTGACCGAGTGGGGC
  AGCCTGGGCGGCGCGACCGTGCCCCTGGAGGTGGTGCTGCTGCGCAAGGTGGGCGCGGCG
  GGCGGCGCGCGCGGCGTCATCCGCCTGCTGGACTGGTTCGAGCGGCCCGACGGCTTCCTG
  CTGGTGCTGGAGCGGCCCGAGCCGGCGCAGGACCTCTTCGACTTTATCACGGAGCGCGGC
  GCCCTGGACGAGCCGCTGGCGCGCCGCTTCTTCGCGCAGGTGCTGGCCGCCGTGCGCCAC
  TGCCACAGCTGCGGGGTCGTGCACCGCGACATTAAGGACGAAAATCTGCTTGTGGACCTG
  CGCTCCGGAGAGCTCAAGCTCATCGACTTCGGTTCGGGTGCGCTGCTCAAGGACACGGTC
  TACACCGACTTCGACGGCACCCGAGTGTACAGCCCCCCGGAGTGGATCCGCTACCACCGC
  TACCACGGGCGCTCGGCCACCGTGTGGTCGCTGGGCGTGCTTCTCTACGATATGGTGTGT
  GGGGACATCCCCTTCGAGCAGGACGAGGAGATCCTCCGAGGCCGCCTGCTCTTCCGGAGG
  AGGGTCTCTCCAGAGTGCCAGCAGCTGATCCGGTGGTGCCTGTCCCTGCGGCCCTCAGAG
  CGGCCGTCGCTGGATCAGATTGCGGCCCATCCCTGGATGCTGGGGGCTGACGGGGGCGCC
  CCGGAGAGCTGTGACCTGCGGCTGTGCACCCTCGACCCTGATGACGTGGCCAGCACCACG
  TCCAGCAGCGAGAGCTTGTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001001852 unedited
  GTTCGGATTTGTATACGACTCATATAGGCGGCNCGCGAATTCGCACGAGGGCGCCTGGGG
  CTCGGGGCTCCGGGGAGGCCGTCGCCCGCGATGCTGCTCTCCAAGTTCGGCTCCCTGGCG
  CACCTCTGCGGGCCCGGCGGCGTGGACCACCTCCCGGTGAAGATCCTGCAGCCAGCCAAG
  GCGGACAAGGAGAGCTTCGAGAAGGCGTACCAGGTGGGCGCCGTGCTGGGTAGCGGCGGC
  TTCGGCACGGTCTACGCGGGTAGCCGCATCGCCGACGGGCTCCCGGTGGCTGTGAAGCAC
  GTGGTGAAGGAGCGGGTGACCGAGTGGGGCAGCCTGGGCGGCGCGACCGTGCCCCTGGAG
  GTGGTGCTGCTGCGCAAGGTGGGCGCGGCGGGCGGCGCGCGCGGCGTCATCCGCCTGCTG
  GACTGGTTCGAGCGGCCCGACGGCTTCCTGCTGGTGCTGGAGCGGCCCGAGCCGGCGCAG
  GACCTCTTCGACTTTATCACGGAGCGCGGCGCCCTGGACGAGCCGCTGGCGCGCCGCTTC
  TTCGCGCAGGTGCTGGCCGCCGTGCGCCACTGCCACAGCTGCGGNGTCGTGCACCGCGAC
  ATTAAGGACGAAAATCTGCTTGTGGACCTGCGCTCCGGAGAGCTCAAGCTCATCGACTTC
  NGGTTCGGGTGCGCTGCTCAAGGACACGGTCTACACCGACTTCGACGGCACCCGAGTGTA
  CAGCCCCCCGGNAGTGGATCCGCTACCACCGCTACCACGGGCGCTCGGCACCGTGTGGTC
  GCTGGGCGTGCTCTCTCATATGGTGTGTGGGGGGACATNCCCTTCAGCAGACGNAGAGAT
  CCTCCGAGGCGCTGTCTCCGNGGGGGAGGTCTTTTCAAGTGCCAGTGAACCCGCGGGGGG
  CTGTCCTGCGCTCAAAAGCCGCGCTGGGCACAATATGGGCATCTGTGCGGGGGGGTCAAA
  GGGCCCCCAAAACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001001852 unedited
  NCGGTATTACTATGNACCGCGGCCCGCAATTCTANATCGAGTTTTTTTTTTTTTTTTTTC
  ACTATGTACAAAAACATTTTAATTGAAATACCTGTATAAAAAAAATGATCTCCAGACATC
  TCACTTTTGAACTGAAAGAACCCCCATCTGCGATGCCTGCACACACCGCATTCACACAAA
  CACAGGTACTGAATAAATTAAACGCTCAGGCTCTGGCCCCACCCCAGCTTTCAGAGCCCA
  CAAGCAGACTGTACAAAGTCAATAATTTAAAACCCAAACCCTGGGCACAGTGCCTGGAAG
  TGTCAGGGTCACCCACTCCCCTTAAGTTAGCCACTATACATGTTCATCTTCTGACAGGCG
  GGGCCAGGACAGACGCCAGGCACAGGAATCAGGGCCTGGGGTCCCTGGACCACAGCCACC
  CCCTCCCCTGCCTCCCCACTGTCCCCTGGGGCTTGGGAGAGGCAGACTGCTCAGAGGAAA
  TAACCTCAACAAATAAATTAAACAATAAATAGCCCCGGTGGGCCGAGGGCACCTCCAGGG
  GGTCACACCATAAATAACAGAGTTGGCGGCGGGTACGGCTCGCGTGGGCGGGCGGGCGCG
  GAGGCCAGGACTTGCATTGTGTGTGCAGGACGTGCCCAGACGCACACCGCAGGACTGAGG
  GCGGGAGGTGGGCTTGGGACCCTGCGCCGGCGGAAAGAGCTCCCGGTGGGCAGGCAGATG
  GGAAAGCCGCCTTCGGACACAGCAGCACAGAAGGGCGTCTGGGGTTCAAGTATCCACCCA
  GGGCAGGCGGGACCTCGACCGAAGCGTCTTTGGACAGACAGAGCTTGAGAAAACAAGTCC
  CGCGGGAACCACGTTTAAAAGGCACTCAAACCGAAGTCACCAGGGGTCAGAGAGTCACTG
  CTCCCA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001001852
• Insert Size: 2000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: The open reading frame of this TrueClone was fully sequenced and found to be a perfect match to the protein associated to this reference.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001001852.2, NP_001001852.1
• RefSeq Size: 2392 bp
• RefSeq ORF: 981 bp
• Locus ID: 415116
• UniProt ID: Q86V86
• Cytogenetics: 22q13.33
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: The protein encoded by this gene belongs to the Ser/Thr protein kinase family, and PIM subfamily. This gene is overexpressed in hematological and epithelial tumors and is associated with MYC coexpression. It plays a role in the regulation of signal transduction cascades, contributing to both cell proliferation and survival, and provides a selective advantage in tumorigenesis. [provided by RefSeq, Jun 2012]