TTC10 (IFT88) (NM_175605) Human Untagged Clone

CAT#: SC128228

IFT88 (untagged)-Human intraflagellar transport 88 homolog (Chlamydomonas) (IFT88), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: IFT88
• Synonyms: D13S1056E; DAF19; hTg737; TG737; TTC10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_175605, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGAAATTCACAAACACTAAGGTACAAATGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATG
  ATCTTTATTCCGGCTATAATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGATGCAGCTTT
  TCAGCAAGCTGTGAGGACTAGTCATGGCAGAAGACCTCCAATAACTGCTAAAATATCAAGCACGGCAGTT
  ACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTGGCATCATCAATAGGAAGACCAATGACAG
  GGGCTATTCAGGATGGAGTTACTAGACCCATGACAGCAGTGAGAGCAGCTGGTTTTACCAAAGCAGCTTT
  GAGAGGCTCTGCATTTGACCCCCTTAGTCAGTCAAGGGGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAA
  GATAGCCCAGAGGAAAAAATAAAGCAATTAGAGAAGGAAGTAAATGAGTTGGTAGAAGAAAGCTGTATTG
  CCAATAGTTGTGGAGACTTAAAATTGGCCTTAGAAAAGGCAAAAGATGCAGGAAGAAAAGAGAGAGTCCT
  GGTGAGACAGCGAGAACAAGTTACAACTCCAGAAAATATCAATTTGGATTTAACTTACTCAGTTCTTTTC
  AATTTGGCCAGTCAGTATTCAGTTAATGAAATGTATGCCGAAGCACTTAACACTTATCAAGTTATAGTCA
  AAAATAAGATGTTTAGCAATGCAGGAATATTGAAAATGAATATGGGAAATATCTATTTAAAGCAAAGAAA
  TTATTCCAAAGCCATTAAATTCTACCGAATGGCATTAGACCAAGTTCCAAGTGTCAATAAGCAAATGAGG
  ATTAAAATAATGCAGAATATTGGAGTTACATTTATTCAGGCTGGTCAGTATTCAGATGCTATTAATTCAT
  ATGAGCACATAATGAGCATGGCACCAAATCTGAAGGCAGGCTACAACCTAACTATCTGTTATTTTGCTAT
  TGGAGACCGAGAAAAAATGAAGAAGGCATTCCAAAAATTGATTACTGTTCCATTAGAAATTGATGAAGAT
  AAATATATTTCACCAAGTGATGATCCTCATACTAACTTAGTAACTGAAGCTATAAAAAATGATCACCTCA
  GGCAAATGGAACGTGAAAGGAAAGCCATGGCAGAAAAATATATTATGACATCTGCAAAACTCATTGCTCC
  TGTAATTGAAACATCTTTTGCTGCAGGTTATGATTGGTGCGTGGAAGTGGTGAAAGCTTCTCAATATGTA
  GAGCTAGCCAATGATCTGGAAATAAACAAAGCAGTTACATACTTGAGACAAAAAGACTATAACCAAGCTG
  TAGAGATCTTAAAAGTGTTGGAAAAAAAGGACAGTAGAGTGAAAAGTGCAGCTGCAACCAATCTCTCAGC
  CCTGTATTATATGGGAAAGGATTTTGCACAAGCCAGCAGCTATGCAGATATAGCTGTGAACTCTGATAGA
  TATAATCCAGCAGCTCTTACTAATAAAGGGAATACAGTTTTTGCAAATGGTGATTATGAGAAGGCCGCTG
  AATTCTATAAAGAGGCTCTAAGAAATGATTCTTCTTGTACTGAAGCACTTTATAATATTGGCCTTACCTA
  TGAGAAACTAAATCGGCTAGATGAGGCTTTGGACTGTTTCCTGAAACTTCACGCAATCCTACGAAACAGT
  GCCGAAGTTCTTTACCAGATAGCAAATATATATGAATTAATGGAAAATCCCAGTCAAGCTATTGAATGGC
  TAATGCAGGTGGTCAGTGTTATTCCAACCGATCCTCAAGTTTTATCTAAGCTAGGAGAATTATATGATCG
  TGAAGGAGATAAATCTCAAGCATTTCAATATTACTATGAGTCATATAGGTATTTTCCTTGTAATATTGAA
  GTCATTGAGTGGCTTGGAGCCTATTACATTGACACCCAATTTTGGGAAAAAGCTATTCAGTACTTTGAAA
  GAGCTTCTCTTATACAGCCTACACAAGTGAAATGGCAGCTGATGGTAGCTAGTTGTTTCAGAAGAAGTGG
  TAACTACCAAAAAGCATTAGATACTTACAAAGATACTCACAGAAAATTTCCAGAAAATGTCGAATGTCTG
  CGTTTCTTAGTTCGTCTCTGCACAGATCTTGGATTAAAAGATGCTCAAGAATATGCCAGAAAACTGAAGA
  GGTTGGAAAAAATGAAAGAAATAAGGGAACAGCGCATAAAGTCAGGCAGAGATGGCAGTGGGGGCTCCCG
  TGGCAAAAGAGAAGGAAGTGCTAGCGGTGATAGTGGCCAGAACTATAGTGCCAGTAGTAAAGGTGAACGA
  CTAAGTGCCAGACTCAGAGCTTTACCTGGGACAAATGAACCTTATGAAAGTAGCAGTAACAAAGAAATAG
  ATGCCTCCTATGTGGACCCACTTGGCCCTCAAATAGAACGACCAAAAACTGCAGCCAAGAAAAGGATCGA
  TGAGGATGATTTTGCTGATGAAGAATTAGGAGATGATTTGCTTCCAGAATAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175605 unedited
  NGGTGGTCAGAATTGTATACGACTCACTATAGGCGGCCGCGATTCGGCACGAGGCTCGGC
  GTCGCGCTTTGGCCAACCGCTGCGTCGTCCCTGGGCCCGAAAACTGTCGCCCGCTTCCCT
  CAGCGTGAGGTACAAATGATGCAAAATGTGCACCTGGCTCCAGAGACAGATGAAGATGAT
  CTTTATTCCGGCTATAATGACTACAATCCAATCTATGATATCGAGGAATTGGAGAATGAT
  GCAGCTTTTCAGCAAGCTGTGAGGACTAGTCATGGCAGAAGACCTCCAATAACTGCTAAA
  ATATCAAGCACGGCAGTTACTAGACCTATAGCTACTGGATATGGGTCCAAGACATCTCTG
  GCATCATCAATAGGAAGACCAATGACAGGGGCTATTCAGGATGGAGTTACTAGACCCATG
  ACAGCAGTGAGAGCAGCTGGTTTTACCAAAGCAGCTTTGAGAGGCTCTGCATTTGACCCC
  CTTAGTCAGTCAAGGGGCCCTGCTTCCCCTTTGGAAGCCAAGAAAAAAGATAGCCCAGAG
  GAAAAAATAAAGCAATTAGAGAAGGAAGTAAATGAGTTGGTAGAAGAAAGCTGTATTGCC
  AATAGTTGTGGAGACTTAAAATTGGCCTTAGAAAAGGCAAAAGATGCAGGAAGAAAAGAG
  AGAGTCCTGGTGAGACAGCGAGAACAAGTTACAACTCCAGAAAATATCAATTTGGATTTA
  ACTTACTCAGTTCTTTTCAATTTGGCCAGTCAGTATTCAGTTAATGAAATGTATGCCGAA
  GCACTTAACACTTATCAAGTTATAGTCAAAAATAAGATGTTTAGCAATGCAGGAATATTN
  GAAATGAATATGGGAAATATCTATNNTAAGCANAGAAATATTCCCNAGCCATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175605 unedited
  CGTTCGCGAGTATCAGTATACCTTNAAATATAAAAAAACAACGTTAAATGCATACATACA
  GAATAGAATACACTTACTTAAGTTTTNGACAGTGAAAAAAAATAATTACAGGTTAGATAT
  TTAATCCAAGGTTTAACATGAGGATGATCTCATAAGGCAATTTCTTTCCTTTAATAAATA
  TTAAAGTGAATATTATTCTGGAAGCAAATCATCTCCTAATTCTTCATCAGCAAAATCATC
  CTCATCGATCCTTTTCTTGGCTGCAGTTTTTGGTCGTTCTATTTGAGGGCCAAGTGGGTC
  CACATAGGAGGCATCTATTTCTTTGTTACTGCTACTTTCATAAGGTTCATTTGTCCCAGG
  TAAAGCTCTGAGTCTGGCACTTAGTCGTTCACCTTTACTACTGGCACTATAGTTCTGGCC
  ACTATCACCGCTAGCACTTCCTTCTCTTTTGCCACGGAAGCCCCCACTGCCATCTCTGCC
  TGACTTTATGCGCTGTAGCCTTATTTCTTTCATTTTTTCCAACCTCTTCAGATTACTGGC
  ATATTCTTGAACATCTTTTAATCCAAGATCTGTGCAAAGACGAACTAAAAAACACAAGAC
  TTCAACATTTTCTGGAAATTTACTGAGAGTATCTTTGAAAGAATCTAATGCTTAATTGAA
  AATACCACTTTTTCTGAAACAACTAGACTCCCTACAGCTGCCATTTAACCTTGTGAAGAC
  TGATTAAAAAAAACCTCTTTAAAAGATCTGAAATAGCTTTTTCCCCAAATTGGAGTGAAA
  ATGTAATAGGACTCCAAACCCCTACCATGACCTACATAATATACAGGGGAAAACCCTATT
  TGGCTAAAAAGTAATATTAGAAATGCTGGAAAATTTTTCTCCCTTCCCGAACCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175605
• Insert Size: 2800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175605.3, NP_783195.2
• RefSeq Size: 3171 bp
• RefSeq ORF: 2502 bp
• Locus ID: 8100
• UniProt ID: Q13099
• Cytogenetics: 13q12.11
• Gene Summary: This gene encodes a member of the tetratrico peptide repeat (TPR) family. The encoded protein is involved in cilium biogenesis. Mutations of a similar gene in mouse can cause polycystic kidney disease. Several transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2017]
  Transcript Variant: This variant (1) encodes the longest isoform (1). Variants 1, 4, 6, 7, and 8 all encode the same isoform (1).