PPP2R2D (NM_018461) Human Untagged Clone

CAT#: SC128054

PPP2R2D (untagged)-Human protein phosphatase 2, regulatory subunit B, delta (PPP2R2D), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPP2R2D
• Synonyms: B55D; B55delta; MDS026
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018461, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCAGGAGCCGGAGGCGGCGGCTGCCCCGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTCA
  AGGGGGCCATCGACGAGGACGTGGCCGAAGCGGACATCATTTCCACCGTTGAGTTTAATTACTCTGGAGA
  TCTTCTTGCAACAGGAGACAAGGGCGGCAGAGTTGTTATTTTTCAGCGTGAACAAGAGAATAAAAGCCGC
  CCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGTCATGAACCGGAGTTTGACTATTTGA
  AAAGTCTAGAAATTGAGGAAAAAATTAATAAAATTAGGTGGTTACCACAACAGAATGCTGCTCATTTTCT
  ACTGTCTACAAATGATAAAACTATAAAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTAT
  AACCTGAAAGACGAAGATGGAAGACTTCGAGACCCATTTAGGATCACGGCGCTACGGGTCCCAATATTGA
  AGCCCATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCATATAAA
  TTCCATTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAGATGACCTGAGAATTAATTTATGGCAC
  TTAGAAATCACAGATAGAAGCTTTAACATCGTGGACATCAAGCCTGCTAACATGGAGGAGCTGACCGAAG
  TCATCACTGCAGCCGAGTTCCACCCGCACCAGTGCAACGTGTTCGTCTACAGCAGTAGCAAAGGGACCAT
  CCGCCTGTGTGACATGCGCTCCTCGGCCCTGTGCGACAGACACTCCAAGTTTTTTGAAGAGCCTGAAGAT
  CCCAGCAGTAGGTCCTTCTTCTCAGAAATAATTTCATCCATATCCGATGTAAAATTCAGTCATAGTGGGC
  GGTACATGATGACCAGAGACTACCTGTCGGTGAAGGTGTGGGACCTCAACATGGAGAGCAGGCCGGTGGA
  GACCCACCAGGTCCACGAGTACCTGCGCAGCAAGCTCTGCTCTCTCTATGAGAACGACTGCATCTTTGAC
  AAGTTTGAGTGTTGCTGGAACGGTTCGGATAGCGCCATCATGACCGGGTCCTATAACAACTTCTTCAGGA
  TGTTTGATAGAGACACGCGGAGGGATGTGACCCTGGAGGCCTCGAGAGAGAGCAGCAAACCGCGCGCCAG
  CCTCAAACCCCGGAAGGTGTGTACGGGGGGTAAGCGGAGGAAAGACGAGATCAGTGTGGACAGTCTGGAC
  TTCAACAAGAAGATCCTGCACACAGCCTGGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATA
  ACTTGTACATATTCCAGGACAAAATCAACTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018461 unedited
  ACGACTCACTATAGGGCGGCCGCGAATTCGCACGAGGCCCCGGCGGCGGCGGCGGCGGCG
  GCGCCGGCGGTGGTGGCGGCCCCGGGGCTGAGCGCTCGGCTGCAGCGGCGCGGAGGCCGT
  CTCCCTGGTCTGCCGCGGTCCCCGCCCGTCCCGCCGCCGGCTGCCATGGCAGGAGCCGGA
  GGCGGCGGCTGCCCCGCGGGCGGCAACGACTTCCAGTGGTGCTTCTCGCAGGTCAAGGGG
  GCCATCGACGAGGACGTGGCCGAAGCGGACATCATTTCCACCGTTGAGTTTAATTACTCT
  GGAGATCTTCTTGCAACAGGAGACAAGGGCGGCAGAGTTGTTATTTTTCAGCGTGAACAA
  GAGAATAAAAGCCGCCCTCATTCTAGGGGAGAATATAATGTTTACAGCACCTTTCAAAGT
  CATGAACCGGAGTTTGACTATTTGAAAAGTCTAGAAATTGAGGAAAAAATTAATAAAATT
  AGGTGGTTACCACAACAGAATGCTGCTCATTTTCTACTGTCTACAAATGATAAAACTATA
  AAATTATGGAAAATAAGTGAACGGGATAAAAGAGCAGAAGGTTATAACCTGAAAGACGAA
  GATGGAAGACTTCGAGACCCATTTAGGATCACGGCGCTACGGGTCCCAATATTGAAGCCC
  ATGGATCTTATGGTAGAAGCGAGTCCACGGCGAATTTTTGCAAATGCTCACACATATCAT
  ATAAATTCCATTTCAGTAAATAGTGATCATGAAACATATCTTTCTGCAAATGACCTGAGA
  AATTAATTATGGCACTTAGAAATCACAGATAGAAGCTTTAACATCGGGGACATCAAGCCT
  GCTAACATGGAGGAGCTGACCGAAGTCATCACTGCAGCCGGAGTTCACCCGGACCAGTGC
  CACGTGTTCGTCTACAGCAAAGCAAAGT
  
  3' Read Nucleotide Sequence

  >Forward primer walk for NM_018461 unedited
  NAAACAGCGAGCACGCGCGCCCGCCTCAACTCGGTAGTGTGTACGGGGGGTAAGCGGNAG
  GAAANACGAGCATCAGTGTGGACAGTCTGGACTTCAACAAAAAGATCCTGCACACAGCCT
  GGCACCCCGTGGACAATGTCATTGCCGTGGCTGCCACCAATAACTTGTACATATTCCAGG
  ACAAAATCAACTAGAGACGCGAACACACAGGAGAAAGCCGCCTCCGCTGGAGGCCCGGTG
  TGGTTCCGCCTCGGCGAGGCGCGAGACAGGCGCTGCTGCTCACGTGGAGACGCTCTCGAA
  GCAGAGTTGACGGACACTGCTCCCAAAAGCGTGGGGGTATGTGTGCNNCATTTCTGTNTC
  NNNNCTGCTNNTCCGTGNNTGGTCTTCCTTCGCACCCCCCCCACCACGGGAGAAGACCCC
  CAGAAACGACAACCCCCCAGCAGACAAACCCACCACCCACTAATTACCCCCCCCCCACAA
  CCGCACCGAACCAATTACACCGGTGCCAAACACCCCCCCCGCACAATACCTTAACAGCAC
  GCCACGCTAAACAGAAGCGAACTCCCACACATCAACACACCCCAAACCCACCCCCCCCAC
  TCGCCACGAATATGTCACCTTTTCCACCCCGACCAAAGAGAGCATATAACCCACCGGAGC
  GGCCTATCACCCCCCCGCCACATGCCGAACCGACACCCAACCGCCCAGTTTTTCAACACG
  AGTTCTCCCTTGTCGCACACCTTATATATTGAAATCCAAACACGAAGCAGCATGACCGCG
  GACACGTTAGCCGANCCACAACTAAAACACGCCTATGCACCCTAGTATCAAAACCCACCG
  AAATACGATGGATAACACGGGTTCCCACAAACAATAGACCAACGCGGTAATAAAAAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018461
• Insert Size: 4700 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018461.2, NP_060931.2
• RefSeq Size: 2105 bp
• RefSeq ORF: 1362 bp
• Locus ID: 55844
• UniProt ID: Q66LE6
• Cytogenetics: 10q26.3
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Tight junction
• Gene Summary: B regulatory subunit of protein phosphatase 2A (PP2A) that plays a key role in cell cycle by controlling mitosis entry and exit. The activity of PP2A complexes containing PPP2R2D (PR55-delta) fluctuate during the cell cycle: the activity is high in interphase and low in mitosis. During mitosis, activity of PP2A is inhibited via interaction with phosphorylated ENSA and ARPP19 inhibitors. Within the PP2A complexes, the B regulatory subunits modulate substrate selectivity and catalytic activity, and also may direct the localization of the catalytic enzyme to a particular subcellular compartment (By similarity).[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) encodes the longer isoform (a).