GDNF Receptor alpha 1 (GFRA1) (NM_005264) Human Untagged Clone

CAT#: SC127971

GFRA1 (untagged)-Human GDNF family receptor alpha 1 (GFRA1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GDNF Receptor alpha 1
• Synonyms: GDNFR; GDNFRA; GFR-ALPHA-1; GFRalpha-1; RET1L; RETL1; TRNR1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127971 sequence for NM_005264 edited (data generated by NextGen Sequencing)
  ATGTTCCTGGCGACCCTGTACTTCGCGCTGCCGCTCTTGGACTTGCTCCTGTCGGCCGAA
  GTGAGCGGCGGAGACCGCCTGGATTGCGTGAAAGCCAGTGATCAGTGCCTGAAGGAGCAG
  AGCTGCAGCACCAAGTACCGCACGCTAAGGCAGTGCGTGGCGGGCAAGGAGACCAACTTC
  AGCCTGGCATCCGGCCTGGAGGCCAAGGATGAGTGCCGCAGCGCCATGGAGGCCCTGAAG
  CAGAAGTCGCTCTACAACTGCCGCTGCAAGCGGGGTATGAAGAAGGAGAAGAACTGCCTG
  CGCATTTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGATCTGCTGGAGGATTCCCCA
  TATGAACCAGTTAACAGCAGATTGTCAGATATATTCCGGGTGGTCCCATTCATATCAGAT
  GTTTTTCAGCAAGTGGAGCACATTCCCAAAGGGAACAACTGCCTGGATGCAGCGAAGGCC
  TGCAACCTCGACGACATTTGCAAGAAGTACAGGTCGGCGTACATCACCCCGTGCACCACC
  AGCGTGTCCAACGATGTCTGCAACCGCCGCAAGTGCCACAAGGCCCTCCGGCAGTTCTTT
  GACAAGGTCCCGGCCAAGCACAGCTACGGAATGCTCTTCTGCTCCTGCCGGGACATCGCC
  TGCACAGAGCGGAGGCGACAGACCATCGTGCCTGTGTGCTCCTATGAAGAGAGGGAGAAG
  CCCAACTGTTTGAATTTGCAGGACTCCTGCAAGACGAATTACATCTGCAGATCTCGCCTT
  GCGGATTTTTTTACCAACTGCCAGCCAGAGTCAAGGTCTGTCAGCAGCTGTCTAAAGGAA
  AACTACGCTGACTGCCTCCTCGCCTACTCGGGGCTTATTGGCACAGTCATGACCCCCAAC
  TACATAGACTCCAGTAGCCTCAGTGTGGCCCCATGGTGTGACTGCAGCAACAGTGGGAAC
  GACCTAGAAGAGTGCTTGAAATTTTTGAATTTCTTCAAGGACAATACATGTCTTAAAAAT
  GCAATTCAAGCCTTTGGCAATGGCTCCGATGTGACCGTGTGGCAGCCAGCCTTCCCAGTA
  CAGACCACCACTGCCACTACCACCACTGCCCTCCGGGTTAAGAACAAGCCCCTGGGGCCA
  GCAGGGTCTGAGAATGAAATTCCCACTCATGTTTTGCCACCGTGTGCAAATTTACAGGCA
  CAGAAGCTGAAATCCAATGTGTCGGGCAATACACACCTCTGTATTTCCAATGGTAATTAT
  GAAAAAGAAGGTCTCGGTGCTTCCAGCCACATAACCACAAAATCAATGGCTGCTCCTCCA
  AGCTGTGGTCTGAGCCCACTGCTGGTCCTGGTGGTAACCGCTCTGTCCACCCTATTATCT
  TTAACAGAAACATCATAG
  
  Clone variation with respect to NM_005264.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005264 unedited
  NNNNNGGGGAAGTTCAAAATTGTAATAGACTCATATAGGCGGCCGCGAAATTCGCACGAG
  GGATAAAGTGAGCCCGGAAAGNGAAGAGGGGGCGGGGACACCATNNTGCCCTGAAANAAT
  AAATAAGTAAATAAACAAACTGGCTCCTCGCCGCAGCTGGACGCGGTCGGTTGAGTCCAG
  GTTGGGTCGGACCTGAACCCCTAAAAGCGGAACCGCCTCCCGCCCTCGCCATCCCGGAGC
  TGAGTCGCCGGCGGCGGTGGCTGCTGCCAGACCCGGAGTTTCCTCTTTCACTGGATGGAG
  CTGAACTTTGGGCGGCCAGAGCAGCACAGCTGTCCGGGGATCGCTGCATGCTGAGCTCCC
  TCGGCAAGACCCAGCGGCGGCTCGGGATTTTTTTNGGGGGGGCGGGGACCAGCCCCGCGC
  CGGCACCATGTTCCTGGCGACCCTGTACTTCGCGCTGCCGCTCTTGGACTTGCTCCTGTC
  GGCCGAAGTGAGCGGCGGAGACCGCCTGGATTGCGTGAAAGCCAGTGATCAGTGCCTGAA
  GGAGCAGAGCTGCAGCACCAAGTACCGCACGCTAAGGCAGTGCGTGGCGGGCAAGGAGAC
  CAACTTCAGCCTGGCATCCGGCCTGGAGGCCAAGGATGAGTGCCGCAGCGCCATGGAGGC
  CCTGAAGCAGAAGTCGCTCTACAACTGCCGCTGCAAGCGGNGTATGAAGAAAGAGAAGAA
  CTGCCTGCGCATTTACTGGAGCATGTACCAGAGCCTGCAGGGAAATGATCTGCTGGAGGA
  TTCCCCATATGAACCAGTTAACAGCAGATTGTCAGATATATTCCGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005264 unedited
  ATGGACCATGGATGATGGCACTTCCAGGNCCAGNANAGCACTGGGGNAGGGTCACAGGGC
  ATGCCACCCGGGCTCTGTTCAGGAAACAGCTATGACCGCGGCCGCAATCTAGAGTCGAGT
  TTTTTTTTTTTTTTTTTCTTTTTACATGTCCATATTGTATTTTTTTAATGCAGCTATGAT
  GTTTCTGTTAAAGATAATAGGGTGGACAGAGCGGTTACCACCAGGACCAGCAGTGGGCTC
  AGACCACAGCTTGGAGGAGCAGCCATTGATTTTGTGGTTATGTGGCTGGAAGCACCGAGA
  CCTTCTTTTTCATAATTACCATTGGAAATACAGAGGTGTGTATTGCCCGACACATTGGAT
  TTCAGCTTCTGTGCCTGTAAATTTGCACACGGTGGCAAAACATGAGTGGGAATTTCATTC
  TCAGACCCTGCTGGCCCCAGGGGCTTGTTCTTAACCCGGAGGGCAGTGGTGGTAGTGGCA
  GTGGTGGTCTGTACTGGGAAGGCTGGCTGCCACACGGTCACATCGGAGCCATTGCCAAAG
  GCTTGAATTGCATTTTTAAGACATGTATTGTCCTTGAAGAAATTCAAAAATTTCAAGCAC
  TCTTCTAGGTCGTTCCCACTGTTGCTGCAGTCACACCATGGGGCCACACTGAGGCTACTG
  GAGTCTATGTAGTTGGGGGTCATGACTGTGCCAATAAGCCCCGAGTAGGCGAGGAGGCAG
  TCAGCGTAGTTTTCCTTTAAACAGCTGCTGACAGACCTTGACTCTGGCTGGCAGTTGGTA
  AAAAAATCCGCAGGCGAGATCTGCAGATGTAATTCGTCTTGCAGGAGTCCTGCCAATCCA
  AACAGTTGGGCTTCTCCCTCTCTTCTAGGAGCCCACGGCACGAC
  
• Restriction Sites: Please inquire
• ACCN: NM_005264
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005264.2, NP_005255.1
• RefSeq Size: 2542 bp
• RefSeq ORF: 1398 bp
• Locus ID: 2674
• UniProt ID: P56159
• Cytogenetics: 10q25.3
• Domains: GDNF
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the glial cell line-derived neurotrophic factor receptor (GDNFR) family of proteins. The encoded preproprotein is proteolytically processed to generate the mature receptor. Glial cell line-derived neurotrophic factor (GDNF) and neurturin (NTN) are two structurally related, potent neurotrophic factors that play key roles in the control of neuron survival and differentiation. This receptor is a glycosylphosphatidylinositol (GPI)-linked cell surface receptor for both GDNF and NTN, and mediates activation of the RET tyrosine kinase receptor. This gene is a candidate gene for Hirschsprung disease. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a). Variants 1 and 6 both encode the same isoform (a). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.