RG9MTD2 (TRMT10A) (NM_152292) Human Untagged Clone

CAT#: SC127692

TRMT10A (untagged)-Human RNA (guanine-9-) methyltransferase domain containing 2 (RG9MTD2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RG9MTD2
• Synonyms: HEL-S-88; MSSGM; MSSGM1; RG9MTD2; TRM10
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127692 sequence for NM_152292 edited (data generated by NextGen Sequencing)
  ATGTCATCTGAAATGTTGCCAGCATTTATTGAAACTTCTAATGTTGACAAAAAGCAAGGC
  ATAAATGAAGATCAAGAGGAGAGCCAGAAGCCAAGATTAGGTGAAGGGTGTGAACCAATA
  TCTAAACGACAAATGAAAAAACTAATAAAACAGAAACAATGGGAAGAGCAACGGGAACTC
  CGCAAACAAAAGCGAAAAGAAAAACGCAAGAGGAAAAAATTAGAGCGACAATGTCAAATG
  GAACCAAACTCAGATGGACATGACAGAAAACGTGTTCGAAGAGATGTTGTTCATAGCACC
  CTTCGCCTTATTATTGACTGTAGTTTTGATCACTTGATGGTATTAAAGGACATTAAGAAA
  CTTCATAAGCAGATTCAACGATGTTACGCAGAAAACCGACGGGCACTGCATCCTGTGCAG
  TTTTACTTGACAAGCCACGGAGGCCAGCTGAAAAAGAACATGGATGAAAATGACAAAGGA
  TGGGTCAACTGGAAGGATATCCATATCAAACCAGAGCACTATAGTGAACTCATAAAGAAA
  GAAGACCTGATTTACCTTACGTCAGATTCACCTAATATACTGAAGGAATTAGATGAATCA
  AAGGCCTATGTGATTGGAGGATTAGTAGATCACAACCATCACAAGGGACTCACATATAAA
  CAAGCGTCAGATTATGGAATCAATCATGCACAGCTCCCACTTGGAAATTTTGTGAAGATG
  AATAGTCGAAAAGTTTTGGCAGTTAATCATGTGTTTGAAATTATTCTGGAATACCTGGAA
  ACAAGAGACTGGCAAGAAGCATTTTTTACTATCTTGCCCCAACGGAAAGGAGCTGTTCCC
  ACAGACAAAGCCTGTGAAAGTGCTTCTCATGACAATCAGTCTGTCAGGATGGAGGAAGGT
  GGATCGGACAGTGATTCCAGTGAGGAGGAATATAGCAGAAATGAACTAGATTCACCACAT
  GAAGAAAAGCAGGATAAGGAAAATCACACTGAATCTACAGTGAACTCTCTGCCACACTAA
  
  Clone variation with respect to NM_152292.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152292 unedited
  CCGCGAATTCGGCACCAGCTGAAATGCGTTTGCTGGCTGGGAGCCTAGAAGGGATAGTGG
  GCGAGGCTCCGCCCCGTGGTGGGGCGCTTTCCGTCGGCGTGTGTTTTGGGGTTGCCATCG
  GCCCGTAGTATGTGAAAATTGTTTTTCAGAGGCACCAAATAATGTCATCTGAAATGTTGC
  CAGCATTTATTGAAACTTCTAATGTTGACAAAAAGCAAGGCATAAATGAAGATCAAGAGG
  AGAGCCAGAAGCCAAGATTAGGTGAAGGGTGTGAACCAATATCTAAACGACAAATGAAAA
  AACTAATAAAACAGAAACAATGGGAAGAGCAACGGGAACTCCGCAAACAAAAGCGAAAAG
  AAAAACGCAAGAGGAAAAAATTAGAGCGACAATGTCAAATGGAACCAAACTCAGATGGAC
  ATGACAGAAAACGTGTTCGAAGAGATGTTGTTCATAGCACCCTTCGCCTTATTATTGACT
  GTAGTTTTGATCACTTGATGGTATTAAAGGACATTAAGAAACTTCATAAGCAGATTCAAC
  GATGTTACGCAGAAAACCGACGGGCACTGCATCCTGTGCAGTTTTACTTGACAAGCCACG
  GAGGCCAGCTGANAAAGAACATGGATGANNAATGACAAGGATGGGTCAACTGGAAGGATA
  TCCATATCANACCAGAGCACTATAGTGAACTCATAAAGAAGAGACCCTGATTACCTACGT
  CAGATCACCTAATTACTGAAGGAATAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_152292 unedited
  CCCCCCCCCCNCCCCCCCCCCCCCCCCNNANACCACCCCTTTACTTAGCACGCGGCCGCA
  TCTANATTTTTTTTTTTTTTTTCTTTTTTCGAGCGTTTATTTAGGTAGTTTAATTCTCTA
  CTTTTATTATGTTCTAATACAAGTAACTTTAGTATTTACTGAAGTTTTAACAAATAACCT
  AAATAAACCAAAACAATGTTGAGAGAAAAGTATACATGCAAATGAGCAAAAAGCTTAATA
  TTTTTAAAACCAAATTTTTAAATACCTCACAAACTAGTTATATCAAAAGAGGTAGTAATC
  TTCACCTTCTTGCCACCAACATCCTAGATACGATATGGGCCTGTAGTGGGTATAATTGCG
  TCCCCCAAAAGCATCCTGTCAAATCCTAACACATGGCACCCTGCAAATGCCACCCTATTC
  GTACACAGGGTCTTTGCTGATGTCACCAACACAGGTCCTACTGCATCATGGCGAACTTTA
  CTCCCACTTTGGCCTAACTACATCCATATCTCCCCCCCCCCTCTCCACCCCCACCTCGCC
  TCCCACTACCCCTCTTCCCTTCCCTTTTTATTCGCTTACCCTACCCCTACTCCTTACACC
  CCCCATGACCATCCCCCCCACCATTACACCCCCCGTTCCCCTCCCCCGCTCCCCCCCCCT
  CCACCCCCCCTCTTCCCCCCCCTTCCCTCATCCCTCCCCCCCCACTTTCCCCTTCACCCC
  GCTCTCCCCCCCCCGCTCCACTCTCTCCCCCCCTCCCCCCCCTCCCCCCCATTTCCCTCC
  TCCTCTCCTCCCCCCCCCCCTCCGAAACTCTAACTCCCTCCTACGTTTATCACTTTTTTT
  CCTCCCCCCCCCCGTCCTTCTTTCCAATACACACTTACACTTCGTATCCCCCCCTTTTTC
  CCCTTTCTACCTTCTCCCCCCCCCTCCCCCCCCGCTTCTTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152292
• Insert Size: 3410 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_152292.2, NP_689505.1
• RefSeq Size: 3711 bp
• RefSeq ORF: 1020 bp
• Locus ID: 93587
• UniProt ID: Q8TBZ6
• Cytogenetics: 4q23
• Domains: DUF425
• Gene Summary: This gene encodes a protein that belongs to the tRNA (Guanine-1)-methyltransferase family. A similar gene in yeast modifies several different tRNA species. Mutations in this gene are associated with microcephaly, short stature, and impaired glucose metabolism. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014]
  Transcript Variant: This variant (1) represents the longest transcript. All three variants encode the same protein.