RHOC (NM_175744) Human Untagged Clone

CAT#: SC127513

RHOC (untagged)-Human ras homolog gene family, member C (RHOC), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RHOC
• Synonyms: ARH9; ARHC; H9; RHOH9
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC127513 sequence for NM_175744 edited (data generated by NextGen Sequencing)
  ATGGCTGCAATCCGAAAGAAGCTGGTGATCGTTGGGGATGGTGCCTGTGGGAAGACCTGC
  CTCCTCATCGTCTTCAGCAAGGATCAGTTTCCGGAGGTCTACGTCCCTACTGTCTTTGAG
  AACTATATTGCGGACATTGAGGTGGACGGCAAGCAGGTGGAGCTGGCTCTGTGGGACACA
  GCAGGGCAGGAAGACTATGATCGACTGCGGCCTCTCTCCTACCCGGACACTGATGTCATC
  CTCATGTGCTTCTCCATCGACAGCCCTGACAGCCTGGAAAACATTCCTGAGAAGTGGACC
  CCAGAGGTGAAGCACTTCTGCCCCAACGTGCCCATCATCCTGGTGGGGAATAAGAAGGAC
  CTGAGGCAAGACGAGCACACCAGGAGAGAGCTGGCCAAGATGAAGCAGGAGCCCGTTCGG
  TCTGAGGAAGGCCGGGACATGGCGAACCGGATCAGTGCCTTTGGCTACCTTGAGTGCTCA
  GCCAAGACCAAGGAGGGAGTGCGGGAGGTGTTTGAGATGGCCACTCGGGCTGGCCTCCAG
  GTCCGCAAGAACAAGCGTCGGAGGGGCTGTCCCATTCTCTGA
  
  Clone variation with respect to NM_175744.4
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_175744 unedited
  TAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCGCAGCTGGAGGCGGCGG
  AGCGGAAGCCTTGACTTCATCTCAGCTCCAGAGCCCGCCCTCTCTTCCTGCAGCCTGGGA
  ACTTCAGCCGGCTGGAGCCCCACCATGGCTGCAATCCGAAAGAAGCTGGTGATCGTTGGG
  GATGGTGCCTGTGGGAAGACCTGCCTCCTCATCGTCTTCAGCAAGGATCAGTTTCCGGAG
  GTCTACGTCCCTACTGTCTTTGAGAACTATATTGCGGACATTGAGGTGGACGGCAAGCAG
  GTGGAGCTGGCTCTGTGGGACACAGCAGGGCAGGAAGACTATGATCGACTGCGGCCTCTC
  TCCTACCCGGACACTGATGTCATCCTCATGTGCTTCTCCATCGACAGCCCTGACAGCCTG
  CAAAACATTCCTGAGAAGTGGCCCCCAGAGGTGAAGCACTTCTGCCCCAACGCGCCCATC
  ATCCTGGTGGGGAATAAGAAGGACCTGAGGCAAGACTAGCCCACCAGGAGAGAGCTGCCC
  AGATGAGCACGAGCCCCGCCGTCCTGAGGAAGGCCGGTCCATGCTTAACCGGATCAGTGC
  CTTTGGCTAACTTGAGCGCCCACCCCCCACCAGGCGGGTACCGGGGCAGGCGCTCCACAT
  CGCCCCCCCCTCTGCCCTCCATCACCCCAGAACCACTGCACCAGGGAATGCCCCTCTCCC
  CTCTCACTCCACGTCCTTTTCTACCCGCCTCCTTCCTTTCCCGGCGTTCTCCATCTCCTC
  TTCCTCCCTCGCTCCCTCTCGGCGACCACCGTCCGGCGGCCCCCACTTTTCCCCCCGCCC
  TGTCTCCCCCCGTCCTCTCCTCCTCCTCCCCGCGCTATACCATCTTCCCCTCCACGTCCC
  CCTCTCCCTCCCCCCTCCCTCTCTCCTCCCTCCCCCACTCCGCCATATCTCCNCTCCTCC
  CTCCCCTCTCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_175744 unedited
  GTACCGCGGCCCGCAATCTAGAGTCGAGTTTTTTTCTTTTTTTTTTACGTTGGAGCCTGT
  AGCCTTTATTCATGCCCCCCTGACCAAATGCAGTGAGAGACAGGCCCCTGCCGAAAACAA
  CTCCAGGGGCCTGGGACTCTGGGTCCCCTACTGCAGACACTTTCCTGTGAGCCAGAAGTG
  TATAAAGTGCTGGTGTGTGACCATCCTTTGGGGAAGGTCAAAGGGGGCAAGATCCCCAGG
  GGCCCTGAGGAAGGGCAGGGCATAGGCGTGGCTTCCACAGCGCTGGGAGGGAGGGCCCGT
  GCCACCACCTCGGGGCTAGAAAACAATGCAGACCTGGGCAGGAGGGAACTGAAAATGGGA
  GCCTTCAGCATGGAGCCCTTAGGAGGCTGGGGTTGTAGGGGGATAATTTCTGTACCCCTG
  TGAAGGGAGGGGGCATGTAGGAAAGGCCTTGGGGATCTCAGAGAATGGGACAGCCCCTCC
  GACGCTTGTTCTTGCGGACCTGGAGGCCAGCCCGAGTGGTCATCTCAAACACCTCCCGCA
  CTACCTCCTTGGTCTTGGCTGAGCACTCAAGGTAGCCAAAGGCACTGATCCCGGTTCCCA
  TGTCCCGGCCTTCCTCAAACCGAACGGACTACTGCTTCATCTTGCGCAGCTCTCTCCTGG
  TGTGCTCGACTTGCACTAAGGACCTCCTTATCCCCCACCCAGATGATGGGCACGTTGCGG
  CAAACGCGCTAAACCTTTGGGTCCCACTTCTAAGAAACGCTCACAGGCCTGTCAGGTCTG
  TCCATGGCAGAACCACATGAGGATGACCTCATTGCCCGGTTAGCACAAGGCCCCAGACCC
  ACCATACCTCTTTGCCCTGTTGGACCCAAAAACCCCTCCACCTGTTGCCGCCCCTTAGGC
  GCCCAAATATTTTAAAACAGTTGGTCCCCGACTCCGCAAAGATCCTCTCCAGCAACGAGG
  GGCTTTTTTTCCCGCTCCCCCCCCGAACCCTATTCTTTGTTT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_175744
• Insert Size: 1140 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175744.3, NP_786886.1
• RefSeq Size: 1116 bp
• RefSeq ORF: 582 bp
• Locus ID: 389
• UniProt ID: P08134
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome
• Gene Summary: This gene encodes a member of the Rho family of small GTPases, which cycle between inactive GDP-bound and active GTP-bound states and function as molecular switches in signal transduction cascades. Rho proteins promote reorganization of the actin cytoskeleton and regulate cell shape, attachment, and motility. The protein encoded by this gene is prenylated at its C-terminus, and localizes to the cytoplasm and plasma membrane. It is thought to be important in cell locomotion. Overexpression of this gene is associated with tumor cell proliferation and metastasis. Multiple alternatively spliced variants, encoding the same protein, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the longest transcript. Variants 1, 2, and 3 encode the same protein.