PTBP2 (NM_021190) Human Untagged Clone

CAT#: SC126811

PTBP2 (untagged)-Human polypyrimidine tract binding protein 2 (PTBP2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PTBP2
• Synonyms: brPTB; nPTB; PTBLP
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC126811 sequence for NM_021190 edited (data generated by NextGen Sequencing)
  ATGGACGGAATCGTCACTGAGGTTGCAGTTGGCGTGAAGAGAGGATCTGACGAACTACTC
  TCAGGCAGTGTTCTCAGTAGTCCGAACTCTAATATGAGCAGCATGGTAGTTACAGCCAAT
  GGTAATGATAGTAAAAAATTTAAAGGAGAAGATAAAATGGATGGTGCTCCTTCTCGTGTA
  CTTCATATTCGAAAATTACCTGGGGAAGTAACAGAAACTGAAGTTATTGCTTTAGGCTTA
  CCTTTTGGTAAGGTGACCAACATCCTTATGCTGAAAGGAAAAAATCAGGCATTTTTGGAA
  CTAGCAACCGAGGAAGCAGCTATTACTATGGTTAATTACTATTCTGCTGTGACACCTCAT
  CTTCGTAACCAACCAATATATATCCAGTACTCGAATCACAAAGAACTAAAGACAGATAAT
  ACATTAAACCAACGTGCTCAGGCAGTTCTTCAAGCTGTGACAGCTGTCCAGACAGCAAAT
  ACTCCTCTTAGTGGCACCACAGTTAGCGAGAGTGCAGTGACTCCAGCCCAGAGTCCAGTA
  CTTAGAATAATTATTGACAACATGTACTACCCTGTAACACTTGATGTTCTTCACCAAATA
  TTTTCTAAGTTTGGTGCTGTATTGAAGATAATCACATTTACAAAAAATAACCAGTTTCAA
  GCTTTGCTCCAGTATGGTGATCCAGTAAATGCTCAACAAGCAAAACTAGCCCTAGATGGT
  CAGAATATTTATAATGCCTGCTGTACCCTAAGGATTGATTTTTCCAAACTTGTGAATTTG
  AATGTAAAATACAACAATGATAAAAGTAGGGATTATACTCGACCTGATCTTCCATCTGGG
  GATGGACAACCTGCATTGGACCCAGCTATTGCTGCAGCATTTGCCAAGGAGACATCCCTC
  TTAGCTGTTCCAGGAGCTCTGAGTCCTTTGGCCATTCCAAATGCTGCTGCAGCAGCTGCT
  GCAGCTGCTGCTGGCCGAGTGGGTATGCCTGGAGTCTCAGCTGGTGGCAATACAGTCCTG
  TTGGTTAGCAATTTAAATGAAGAGATGGTTACGCCCCAAAGTCTGTTTACCCTCTTCGGT
  GTTTATGGAGATGTGCAGCGTGTGAAGATTTTATACAATAAGAAAGACAGCGCTCTAATA
  CAGATGGCTGATGGAAACCAATCACAACTTGCCATGAATCATCTTAATGGACAGAAAATG
  TATGGAAAAATTATTCGTGTTACTCTGTCTAAACATCAGACTGTACAGCTACCTCGAGAG
  GGACTTGATGATCAAGGGCTAACAAAAGATTTTGGTAATTCCCCATTGCATCGTTTTAAG
  AAACCTGGATCCAAAAATTTTCAAAACATTTTTCCTCCTTCTGCCACCCTTCACCTATCT
  AATATCCCTCCATCAGTAGCAGAAGAGGATCTACGAACACTGTTCGCTAACACTGGGGGC
  ACTGTGAAAGCATTTAAGTTTTTTCAAGATCACAAAATGGCTCTTCTTCAGATGGCAACA
  GTGGAAGAAGCTATTCAGGCCTTGATTGATCTTCATAATTATAACCTTGGAGAAAACCAT
  CATCTGAGAGTGTCTTTCTCCAAGTCAACAATTTAA
  
  Clone variation with respect to NM_021190.2
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021190 unedited
  TTGTAATACGACTCACTTATAGGGCGGCCGCGATTCGGCACGAGGTCCGGTTCGGCAATG
  GACGGAATCGTCACTGAGGTTGCAGTTGGCGTGAAGAGAGGATCTGACGAACTACTCTCA
  GGCAGTGTTCTCAGTAGTCCGAACTCTAATATGAGCAGCATGGTAGTTACAGCCAATGGT
  AATGATAGTAAAAAATTTAAAGGAGAAGATAAAATGGATGGTGCTCCTTCTCGTGTACTT
  CATATTCGAAAATTACCTGGGGAAGTAACAGAAACTGAAGTTATTGCTTTAGGCTTACCT
  TTTGGTAAGGTGACCAACATCCTTATGCTGAAAGGAAAAAATCAGGCATTTTTGGAACTA
  GCAACCGAGGAAGCAGCTATTACTATGGTTAATTACTATTCTGCTGTGACACCTCATCTT
  CGTAACCAACCAATATATATCCAGTACTCGAATCACAAAGAACTAAAGACAGATAATACA
  TTAAACCAACGTGCTCAGGCAGTTCTTCAAGCTGTGACAGCTGTCCAGACAGCAAATACT
  CCTCTTAGTGGCACCACAGTTAGCGAGAGTGCAGTGACTCCAGCCCAGAGTCCAGTACTT
  AGAATAATTATTGACAACATGTACTACCCTGTAACACTTGATGTTCTTCACCAAATATTT
  TCTAAGTTTGGTGCTGTATTGAAGATAATCACATTTACAAAAAATAACCAGTTTCAAGCT
  TTGCTCCAGTATGGGTGATCCAGTAAATGCTCAACAAGCAAAACTAGCCCTAGATGGTCA
  GAATATTTATAATGCCTGCTGTACCCTAAGGATTGATTTTTCCAAACTTGTGAAATTTGA
  TGTAAAATACACCAATGATAAGTAGGGATATACTCGACCTGATCTTCCTCTGGGGATGGA
  CACCTGCATTGGACCACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021190 unedited
  GGTTTACTTCCGGCCGCAAGGCCTGGCAAGGGTGAGATTAGTACTGGCTTTCGCACCGTG
  GGCGAGAGAATAAACATGAATTGGGAAGCCGGTGGAGGGAAAACAAATAAGAGGAGAGAG
  AAAAGGCGTGCAAAGAAAGACAGAAAAGGTGAATATGGAGCTACAGAAGTGTTTATTGAG
  CATGCTACAGGGGTAAGCATATTACACACAAAATGAAATTAAACAACCATCAAACCTCCC
  AACTTTGTTAGAAAATTAATCCTTAATGGTGCCGGTTTCAAACTATACTGAATTTTACAT
  TTCTAAAGATGTAAAAACTCAACTAATAGAAAATATGCATGACCATTCTGTCTACATAGA
  TAACAGAATCTATGAATCTTGAAACTAAGTACATGAATTTCAAAAAGTATTGGCCATTTA
  AACAGAATCAACAGTTCGGATTGACAAGAACTGTCCAAATTAATGGGACCTGTAGATTCT
  GAGCCATGTTTTTATTAAAGTCAGATCTATTCCTAAATACAAAATATATGGAAGATTTAT
  TAAAACTGAATATTACAATGCAAGGTTAATTAGGACTACATGCACATAAACTTTGGTCCC
  ACCTGGGTGTCAGGTTTAGAAAACTGCCACAACACTGAATCTTCCTGCATATTGACGCAG
  TACACTTTTTTTATCTTTGAAAAAACATGCACCGAGTCCTGAACTAGCTAGACCAGTTTG
  CACATGCTCATAAATACACGACCATATTCCAGACCTTAATACAAACTACAGATGGCAAAT
  ATACTCAGATGCCGCAAGTCATGTCACTTTAAAAAATCTACTAAAAAATTCAGCCACAAC
  AAGAATTACCTAGGGCTGACTTCATATGAACGTCCACAAGATGGGTACCAAAGGTTAGAC
  CCCCCATGGCTTTCCCAAGGCTCAACCTCCCGAATCCTTGGTGGTTCAGAATAAAACCTT
  GCTTTGTTATTTGACTAAGGCTCTCTTACTGATTAGACAGATTGGATATCAAAGG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021190
• Insert Size: 3460 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021190.1, NP_067013.1
• RefSeq Size: 3054 bp
• RefSeq ORF: 1596 bp
• Locus ID: 58155
• UniProt ID: Q9UKA9
• Cytogenetics: 1p21.3
• Domains: RRM
• Gene Summary: The protein encoded by this gene binds to intronic polypyrimidine clusters in pre-mRNA molecules and is implicated in controlling the assembly of other splicing-regulatory proteins. This protein is very similar to the polypyrimidine tract binding protein (PTB) but most of its isoforms are expressed primarily in the brain. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2014]
  Transcript Variant: This variant (6, also known as nPTB1) uses two alternate in-frame splice sites in the 3' coding region, compared to variant 1. The encoded isoform (6) is shorter than isoform 1.