TTLL12 (NM_015140) Human Untagged Clone

CAT#: SC122822

TTLL12 (untagged)-Human tubulin tyrosine ligase-like family, member 12 (TTLL12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TTLL12
• Synonyms: dJ526I14.2
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_015140 edited
  CTGGAGTCGGCGCGGGTGCTGGCGCCATGGAGGCCGAGCGGGGTCCCGAGCGCCGGCCTG
  CGGAGCGTAGCAGCCCGGGCCAGACGCCGGAGGAGGGCGCGCAGGCCTTGGCCGAGTTCG
  CGGCGCTGCACGGCCCGGCGCTGCGCGCTTCGGGGGTCCCCGAACGTTACTGGGGCCGCC
  TCCTGCACAAGCTGGAGCACGAGGTTTTCGACGCTGGGGAAGTGTTTGGGATCATGCAAG
  TGGAGGAGGTAGAAGAGGAGGAGGACGAGGCAGCCCGGGAGGTGCGGAAGCAGCAGCCCA
  ACCCGGGGAACGAGCTGTGCTACAAGGTCATCGTGACCAGGGAGAGCGGGCTCCAGGCAG
  CCCACCCCAACAGCATCTTCCTCATCGACCACGCCTGGACGTGCCGTGTGGAGCACGCGC
  GCCAGCAGCTGCAGCAGGTGCCCGGGCTGCTGCACCGCATGGCCAACCTGATGGGCATTG
  AGTTCCACGGTGAGCTGCCCAGTACAGAGGCTGTGGCCCTGGTGCTGGAGGAGATGTGGA
  AGTTCAACCAGACCTACCAGCTGGCCCATGGGACAGCTGAGGAGAAGATGCCGGTGTGGT
  ATATCATGGACGAGTTCGGTTCGCGGATCCAGCACGCGGACGTGCCCAGCTTCGCCACGG
  CACCCTTCTTCTACATGCCGCAGCAGGTGGCCTACACGCTGCTGTGGCCCCTGAGGGACC
  TGGACACTGGCGAGGAGGTGACCCGAGACTTTGCCTACGGAGAGACGGACCCCCTGATCC
  GGAAGTGCATGCTGCTGCCCTGGGCCCCCACCGACATGCTGGACCTCAGCTCTTGCACAC
  CCGAGCCGCCCGCCGAGCACTACCAGGCCATTCTGGAGGAAAACAAGGAGAAGCTGCCAC
  TTGACATCAACCCCGTGGTGCACCCCCACGGCCACATCTTCAAGGTCTACACGGACGTGC
  AGCAGGTGGCCAGCAGCCTCACCCACCCGCGCTTCACCCTCACCCAGAGTGAGGCGGACG
  CCGACATCCTCTTCAACTTCTCACACTTCAAGGACTACAGGAAACTCAGCCAGGAGAGGC
  CAGGCGTGCTGCTGAACCAGTTCCCCTGCGAGAACCTGCTGACTGTCAAGGACTGCCTGG
  CCTCCATCGCGCGCCGGGCAGGTGGCCCCGAGGGCCCACCCTGGCTGCCCCGAACCTTCA
  ACCTGCGCACTGAGCTGCCCCAGTTTGTCAGCTACTTCCAGCAGCGGGAAAGGTGGGGCG
  AGGACAACCACTGGATCTGCAAGCCCTGGAACCTGGCGCGCAGCCTGGACACCCACGTCA
  CCAAGAGCCTGCACAGCATCATCCGGCACCGAGAGAGCACCCCCAAGGTTGTGTCCAAGT
  ACATCGAAAGTCCCGTGTTGTTCCTTCGAGAAGACGTGGGAAAGGTCAAGTTCGACATCC
  GCTACATCGTGCTGCTGCGGTCAGTGAGGCCCCTACGGTTGTTCGTGTATGATGTGTTCT
  GGCTGCGGTTCTCCAACCGGGCCTTTGCACTCAACGACCTGGATGACTACGAGAAGCACT
  TCACGGTCATGAACTATGACCCGGATGTGGTGCTGAAGCAGGTGCACTGTGAAGAGTTCA
  TCCCCGAGTTTGAGAAGCAATACCCAGAATTTCCCTGGACGGACGTCCAGGCTGAGATCT
  TCCGGGCCTTCACGGAGCTGTTCCAGGTGGCCTGTGCCAAGCCACCACCCCTGGGCCTCT
  GCGACTACCCCTCATCCCGGGCCATGTATGCCGTCGACCTCATGCTGAAGTGGGACAACG
  GCCCAGATGGAAGGCGGGTGATGCAGCCGCAGATCCTGGAGGTGAACTTCAACCCCGACT
  GTGAGCGAGCCTGCAGGTACCACCCCACCTTCTTCAACGACGTCTTCAGCACCTTGTTTC
  TGGACCAGCCCGGTGGCTGCCACGTTACCTGCCTTGTCTAGGCACTCGCTGTCCCCAAAA
  CCTGTGCTTGGGGCAGGATTCCAACCTCAGTTCTCTGAGCTGCTTCTGCAAAGGCCCCCA
  TGTCCCTCCCCACACCGGCCCTGGGCATAGCCTCAGCCCCAGGCCTCTGTCCTGCCGAGC
  CATCCTCCCGGCGCCACACTCCGGGAGCACAGCATCCTCCTCTCACCTGTGGGTCAGAGC
  AGGACAGTGATGGTGTCCCCAGGGCTGAGCACCACCCCACGCCCTGCCCTCACCCCTCAC
  CACCATCTGTGCACTGATGAGTCTCCAGTTTAGCCAAGGGCTTCGTTCCTGGCATGGAGA
  ATTTGTTCCTGGCTGCTGTGTTTCCAGGGGGTGCTGGGGGAAGGGTTCCGTGGAGCGAGA
  CAAGGTGTCCTCGGGAGCAGGGTTCCACCGGGAAGCGTTTGGGAGCCCTGTATCACACGG
  GGCAGGCGGGTTTCTCTTCCGGGGTCTCTGCTCTTATGCATCAGGACGACCCCGGGACGG
  CTGTGGGGCCCCACACTGCACCCACAGGGCTCTATGCGACAGGGGCCCAGGAACAGCCTG
  AGGCCACCACCCAGCAAGCCCGCCTTATCACCCATTCCAGCTCACCCAGAACCTTCACCA
  GCAAACCTCCTGCTGAGGTCCTGGCAGGAGGCCACCGTCTTGTTACCGTTTCCTTTTCGT
  TTGCTGAGGGTCACAGACCCCAACAGGGAAATCAGTATCTGTCTTCCCAGTGGTTGCCCT
  GCTCGCCGGGCACTCCACGGGGTCCCGCCCTTGTGTGAGATGGGCCAGGATCCTTCGGCA
  AGGGGCGCCTGGGGCTGGGGCTGATTGTGGGCGGTGGAGCGCCAGACAGAAAAGGATTCC
  AATGAGAACTTCAGGTTAAAGTCAGATGCCACCTACCAGGGTCTACAGTCAAAATGTTGG
  CTTTTTCTTATTTTTTAATGTATGGGAGAAAAATGTAAAATTCCAGTTCTTTTCTAATTG
  TGTTTCTGAAATTAGGAGTCAGCTGCCAGCGTTTTTGTGTGGCTGCAGTGTGCCTGGGCC
  CAGCTCACGGGCAGTGGGTGGACCTAACTGCCCAGGCAGGCGAGAGCTACTTCCAGAGCC
  TTCCAGTGCATGGGAGGGCAGGGCTAGGTGTAGCGGTGTCTCCTCTTTGAAATTAAGAAC
  TATCTTTCTTGTAGCAAAGCTGCACCTGATGATGCTGCCTCTCCTCTCTGTGTTGTCTGG
  GCCCTTGTTTACAAGCACGCGTTACCCTTCCTGAGGGGAGCCATGCTCTAGCCCCTGGAG
  GGCCTGTTGCAGGGGCAGGGCGGGCCCGTCGCCTTTGGCAGCTCCTGGAGAGCTGTGGAC
  ATGCAGTCCCCCTCAGTTCGTGCTGCAATAAAGGCCATCTTCTCTTAAAAAAAAAAAAAA
  AAAAAAAAAAAAAAAAAAAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_015140 unedited
  ATGTCATTTTTGTATACGACTCATATAGGGCGGCCGCGAATTCGCACGAGGCTGGAGTCG
  GCGCGGGTGCTGGCGCCATGGAGGCCGAGCGGGGTCCCGAGCGCCGGCCTGCGGAGCGTA
  GCATCCCGGGCCAGACGCCGGAGGAGGGCGCGCAGGCCTTGGCCGAGTTCGCGGCGCTGC
  ACGGCCCGGCGCTGCGCGCTTCGGGGGTCCCCGAACGTTACTGGGGCCGCCTCCTGCACA
  AGCTGGAGCACGAGGTTTTCGACGCTGGGGAAGTGTTTGGGATCATGCAAGTGGAGGAGG
  TAGAAGAGGAGGAGGACGAGGCAGCCCGGGAGGTGCGGAAGCAGCAGCCCAACCCGGGGA
  ACGAGCTGTGCTACAAGGTCATCGTGACCAGGGAGAGCGGGCTCCAGGCAGCCCACCCCA
  ACAGCATCTTCCTCATCGACCACGCCTGGACGTGCCGTGTGGAGCACGCGCGCCAGCAGC
  TGCAGCATGTGCCCGGGCTGCTGCACCGCATGGCCAACCTGATGGGCATTGAGTTCCACG
  GTGAGCTGCCCAGTACAGAGGCTGTGGCCCTGGTGCTGGAGGAGATGTGGAAGTTCAACC
  AGACCTACCAGCTGGCCCATGGGACAGCTGAGGAGAAGATGCCGGTGTGGTATATCATGG
  ACGAGTTCGGTTCGCGGATCCAGCACGCGGACGTGCCCAGCTTCGCCACGGCACCCTTCT
  TCTACATGCCGCATCATGTGGCCTACACGCTGCTGTGGCCCCTGAGGGACCTGGACACTG
  GCGAGGAGGTGACCCGAGACTTTGCCTACGGAGAGACCGACCCCCTGATCCGGAAGTGCA
  TGCTGGTGCCCTGGGCCCCCACCGAATGCTGGACCTCAGCTCTTGCCACCCGAGCG
  
• Restriction Sites: Please inquire
• ACCN: NM_015140
• Insert Size: 3394 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_015140.2, NP_055955.1
• RefSeq Size: 3353 bp
• RefSeq ORF: 1935 bp
• Locus ID: 23170
• UniProt ID: Q14166
• Cytogenetics: 22q13.2
• Gene Summary: Negatively regulates post-translational modifications of tubulin, including detyrosination of the C-terminus and polyglutamylation of glutamate residues (PubMed:20162578, PubMed:23251473). Also, indirectly promotes histone H4 trimethylation at 'Lys-20' (H4K20me3) (PubMed:23251473). Probably by controlling tubulin and/or histone H4 post-translational modifications, plays a role in mitosis and in maintaining chromosome number stability (PubMed:20162578, PubMed:23251473). During RNA virus-mediated infection, acts as a negative regulator of the DDX58/RIG-I pathway by preventing MAVS binding to TBK1 and IKBKE (PubMed:28011935).[UniProtKB/Swiss-Prot Function]