SEMA5B (BC002776) Human Untagged Clone

CAT#: SC122350

(untagged)-Human cDNA clone MGC:3430 IMAGE:3621447, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: SEMA5B
• Synonyms: SEMAG; SemG
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC002776 edited
  GGCACGAGGCTCCAGCATGGAGGAGGCCACCGGCTGTGCAGGGTTCAATCTCATCCACTT
  GGTGGCCACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCT
  GTCTTGCCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCC
  CAACCATTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGA
  ATTCAAGACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATT
  GCAGCAGACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTT
  CCGGCCCGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTGATACCGCCGTCCTGG
  GGACTTGGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCA
  GTTTGGTTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCC
  ATCCGGCTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCT
  GACATATGGCCGCAGGTCCAGTTCAGCCCAGGTCTCTCATGGTTATCTTCCAACCCACTG
  TCACGCTGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTGAGGAATTG
  GAGAATGGAGATGGCAAGAGGGCAGGCTTTTAAGTTTGGGTTGGAGACAACTTCCTGTGG
  CCCCCACAAGCTGAGTCTGGCCTTCTCCAGCTGGCCCCAAAAAAGGCCTTTGCTACATCC
  TGATTATCTCTGAAAGTAATCAATCAAGTGGCTCCAGTAGCTCTGGATTTTCTGCCAGGG
  CTGGGCCATTGTGGTGCTGCCCCAGTATGACATGGGACCAAGGCCAGCGCAGGTTATCCA
  CCTCTGCCTGGAAGTCTATACTCTACCCAGGGCATCCCTCTGGTCAGAGGCAGTGAGTAC
  TGGGAACTGGAGGCTGACCTGTGCTTAGAAGTCCTTTAATCTGGGCTGGTACAGGCCTCA
  GCCTTGCCCTCAATGCACGAAAGGTGGCCCAGGAGAGAGGATCAATGCCATAGGAGGCAG
  AAGTCTGGCCTCTGTGCCTCTATGGAGACTATCTTCCAGTTGCTGCTCAACAGAGTTGTT
  GGCTGAGACCTGCTTGGGAGTCTCTGCTGGCCCTTCATCTGTTCAGGAACACACACACAC
  ACACACTCACACACGCACACACAATCACAATTTGCTACAGCAACAAAAAAGACATTGGGC
  TGTGGCATTATTAATTAAAGATGATATCCAGTCAAAAAAAAAAAAAAAAAAAAAAAAAAA
  AAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC002776 unedited
  NAAAATTCGGTCCGATCTTGTATAACACTCACTAAGGCGGCCGCGAAATNCGCACGAGGC
  CTCCAGCATGNAGGAGGCCAACCGGCTTGTGCAGGGTTCAATCTCATCCACTTGGTGGCC
  AACGGGCATCTCCTGCTTCTTGGGCTCTGGGCTCCTGACCCTAGCAGTGTACCTGTCTTG
  CCAGCACTGCCAGCGTCAGTCCCAGGAGTCCACACTGGTCCATCCTGCCACCCCCAACCA
  TTTGCACTACAAGGGCGGAGGCACCCCGAAGAATGAAAAGTACACACCCATGGAATTCAA
  GACCCTGAACAAGAATAACTTGATCCCTGATGACAGAGCCAACTTCTACCCATTGCAGCA
  GACCAATGTGTACACGACTACTTACTACCCAAGCCCCCTGAACAAACACAGCTTCCGGCC
  CGAGGCCTCACCTGGACAACGGTGCTTCCCCAACAGCTACTACCGCCGTCCTGGGGACTT
  GGGCTTCTTGCCTTCATAAGGCACAGAGCAGATGGAGATGGGACAGTGGAGCCAGTTTGG
  TTTTCTCCCTCTGCACTAGGCCAAGAACTTGCTGCCTTGCCTGTGGGGGGTCCCATCCGG
  CTTCAGAGAGCTCTGGCTGGCATTGACCATGGGGGAAAGGGCTGGTTTCAGGCTGACATA
  TGGCCGCAGGTCCAGTTCAGCCCCAGTCTCTCATGGTTATCTTCCAACCCACTGTCACGC
  TGACACTATGCTGCCATGCCTGGGCTGTGGACCTACTGGGCATTTTGAGAATTGGAGAAT
  GGAGAATGGCAAGAGGGCAGGCTTTTTAGTTTTGGGTTGGAGACAACTTTTCTGTGGCCC
  CCCACAAGCTGAAGTCTGGCCTTCTCCAACTTGCCCCAAAAAAAGCCTTA
  
• Restriction Sites: Please inquire
• ACCN: BC002776
• Insert Size: 1383 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC002776.1, AAH02776.1
• RefSeq Size: 1383 bp
• Locus ID: 54437
• Cytogenetics: 3q21.1
• Protein Families: Transmembrane
• Protein Pathways: Axon guidance
• Gene Summary: This gene encodes a member of the semaphorin protein family which regulates axon growth during development of the nervous system. The encoded protein has a characteristic Sema domain near the N-terminus, through which semaphorins bind to plexin, and five thrombospondin type 1 repeats in the C-terminal region of the protein. The protein product may be cleaved and exist as a secreted molecule (PMID: 19463192). Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]