WNK1 (BC021121) Human Untagged Clone

CAT#: SC122349

(untagged)-Homo sapiens, clone MGC:31983 IMAGE:4653173, complete cds

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: WNK1
• Synonyms: HSAN2; HSN2; KDP; p65; PPP1R167; PRKWNK1; PSK
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for BC021121 edited
  GGTCTCCTCTCCTCTGCCCCGGGGCCGCCACCGCCCGGGCGTCGGACCTGGTCCCGTGCT
  CGCGGTGCCGCCGCCCTCTGGGCCTAGCCCGCCCAGCTCGGCGAGCGGCGGCAGTGGGAG
  CCGCGTCCGCCGCATCCGCCTCGACTCGGTGCCGGCCCCTGGCCCTCCCCTCATGACTGC
  GGCGCCTCTGCTGCCACCGCCCGCCCGGCCGCCGCTCGCCGCAGGATGGATGCGGACCGT
  GCGGCGCTAACCCCCGTGGCTCAGCTCCCGAATCGCCCGCCTTCGAGCCCTCCTCGTGAG
  CCGCAGCAGCCTCGGTGCCAGCCCCCGCCGCAGCTGGGCCCAGCGGTCCGCCTGTCCCTC
  GTTGCGGCTTGTCGGTGCTGAGTGAGGCGTCGTCCGGGTCGGCGCGAACCCGCCCGGCCG
  CGGTGCCCTGCAGACCTCTGCGCGGGCGGCTCGGCCCTTCACGCCCTTTTCGTTCACGAA
  TCCGAGCCCGCTCGCCTCTCTCCAGCGAACCGACCATGTCTGGCGGCGCCGCAGAGAAGC
  AGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTGCCCCCAAGAATGGCT
  CCAGCTCCGATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCGCCGACGCTGTGACCG
  GCAGGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGGACAGCCGTGGGGCGG
  CCGCGACCACTACCACCACTGAGCACCGCTTCTTCCGCCGGAGCGTCATCTGCGACTCCA
  ATGCCACTGCACTGGAGCTTCCCGGCCTTCCTCTTTCCCTGCCCCAGCCCAGCATCCCCG
  CGGCTGTCCCGCAGAGTGCTCCACCGGAGCCCCACCGGGAAGAGACCGTGACCGCCACCG
  CCACTTCCCAGGTAGCCCAGCAGCCTCCAGCCGCTGCCGCCCCTGGGGAACAGGCCGTCG
  CGGGCCCTGCCCCCTCGACTGTCCCCAGCAGTACCAGCAAAGACCGCCCAGTGTCCCAGC
  CTAGCCTTGTGGGGAGCAAAGAGGAGCCGCCGCCGGCGAGAAGTGGCAGCGGCGGCGGCA
  GCGCCAAGGAGCCACAGGAGGAACGGAGCCAGCAGCAGGATGATATCGAAGAGCTGGAGA
  CCAAGGCCGTGGGAATGTCTAACGATGGCCGCTTTCTCAAGTTTGACATCGAAATCGGCA
  GAGGCTCCTTTAAGACGGTCTACAAAGGTCTGGACACTGAAACCACCGTGGAAGTCGCCT
  GGTGTGAACTGCAGGATCGAAAATTAACAAAGTCTGAGAGGCAGAGATTTAAAGAAGAAG
  CTGAAATGTTAAAAGGTCTTCAGCATCCCAATATTGTTAGATTTTATGATTCCTGGGAAT
  CCACAGTAAAAGGAAAGAAGTGCATTGTTTTGGTGACTGAACTTATGACGTCTGGAACAC
  TTAAAACGTATCTGAAAAGGTTTAAAGTGATGAAGATCAAAGTTCTAAGAAGCTGGTGCC
  GTCAGATCCTTAAAGGTCTTCAGTTTCTTCATACTCGAACTCCACCTATCATTCACCGCG
  ATCTTAAATGTGACAACATCTTTATCACCGGCCCTACTGGCTCAGTCAAGATTGGAGACC
  TCGGTCTGGCAACCCTGAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTATGTTTCAGG
  TGTACCTTGGAGCCTGACTGGCTTTCTGACATTCCTTTTATTTAGAATCCTGGCTCTGTC
  AGAGTTTTAAGTGATATAAACCTTAAGAAATTCATAGCTTGAACTCAGGAGGTGATCCAT
  TGTACTTATGAGATATAGGATTCTCTATATTTGTGCTTCTTGGAATCATCTTAGAGACTA
  TTGGTAACATTACGTTACAAGAAATAAAGTGAACTTTGTTGATAAAAAAAAAAAAAAAAA
  AAAAAAAA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for BC021121 unedited
  ACCCATTCGCACNGGGGTCTCCTTTTCTCTGCCCCGGGGCCGCCACCGCCCGGGCGTCGG
  ACCTGGTCCCGTGCTCGCGGTGCCGCCGCCCTCTGGGCCTAGCCCGCCCAGCTCGGCGAG
  CGGCGGCAGTGGGAGCCGCGTCCGCCGCATCCGCCTCGACTCGGTGCCGGCCCCTGGCCC
  TCCCCTCATGACTGCGGCGCCTCTGCTGCCACCGCCCGCCCGGCCGCCGCTCGCCGCAGG
  ATGGATGCGGACCGTGCGGCGCTAACCCCCGTGGCTCAGCTCCCGAATCGCCCGCCTTCG
  AGCCCTCCTCGTGAGCCGCAGCAGCCTCGGTGCCAGCCCCCGCCGCAGCTGGGCCCAGCG
  GTCCGCCTGTCCCTCGTTGCGGCTTGTCGGTGCTGAGTGAGGCGTCGTCCGGGTCGGCGC
  GAACCCGCCCGGCCGCGGTGCCCTGCAGACCTCTGCGCGGGCGGCTCGGCCCTTCACGCC
  CTTTTCGTTCACGAATCCGAGCCCGCTCGCCTCTCTCCAGCGAACCGACCATGTCTGGCG
  GCGCCGCAGAGAAGCAGAGCAGCACTCCCGGTTCCCTGTTCCTCTCGCCGCCGGCTCCTG
  CCCCCAAGAATGGCTCCAGCTCCCATTCCTCCGTGGGGGAGAAACTGGGAGCCGCGGCCG
  CCGACGCTGTGACCGGGCAGACCGAGGAGTACAGGCGCCGCCGCCACACTATGGACAAGG
  ACAGCCGTGGGGCGGCCGCGACCACTACCACCACTGAGCACCGCTTTCTTCGCCCGAGCG
  TCATTTGCGACTCCCAAGCCACTGCACTGGAGCTTTCCGGCCTTTCCTTTTTCCTGCCCC
  AGCA
  
• Restriction Sites: Please inquire
• ACCN: BC021121
• Insert Size: 1928 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: BC021121.1
• RefSeq Size: 1928 bp
• Locus ID: 65125
• Cytogenetics: 12p13.33
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the WNK subfamily of serine/threonine protein kinases. The encoded protein may be a key regulator of blood pressure by controlling the transport of sodium and chloride ions. Mutations in this gene have been associated with pseudohypoaldosteronism type II and hereditary sensory neuropathy type II. Alternatively spliced transcript variants encoding different isoforms have been described but the full-length nature of all of them has yet to be determined.[provided by RefSeq, May 2010]