Aurora C (AURKC) (NM_003160) Human Untagged Clone

CAT#: SC118139

AURKC (untagged)-Human aurora kinase C (AURKC), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Aurora C
• Synonyms: AIE2; AIK3; ARK3; AurC; aurora-C; HEL-S-90; SPGF5; STK13
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_003160 edited
  ATGCGGCGCCTCACAGTCGATGACTTTGAAATCGGGCGTCCCCTGGGCAAGGGGAAATTT
  GGGAATGTGTACCTGGCTCGGCTCAAGGAAAGCCATTTCATTGTGGCCCTGAAGGTTCTC
  TTCAAGTCGCAGATAGAGAAGGAAGGACTGGAGCACCAGCTGCGCCGGGAAATTGAGATC
  CAGGCTCATCTACAACACCCCAATATCCTGCGCCTGTATAACTATTTCCATGATGCACGC
  CGGGTGTACCTGATTCTGGAATATGCTCCAAGGGGTGAGCTCTACAAGGAGCTGCAGAAA
  AGCGAGAAATTAGATGAACAGCGCACAGCCACGATAATAGAGGAGTTGGCAGATGCCCTG
  ACCTACTGCCATGACAAGAAAGTGATTCACAGAGATATTAAGCCAGAGAACCTGCTGCTG
  GGGTTCAGGGGTGAGGTGAAGATTGCAGATTTTGGCTGGTCTGTGCACACCCCCTCCCTG
  AGGAGGAAGACAATGTGTGGGACACTGGACTACTTGCCGCCAGAAATGATTGAGGGGAGA
  ACATATGATGAAAAGGTGGATTTGTGGTGCATTGGAGTGCTCTGCTATGAGCTGCTGGTG
  GGATATCCACCCTTTGAGAGCGCCTCCCACAGTGAGACTTACAGACGCATCCTCAAGGTA
  GATGTGAGGTTTCCACTATCAATGCCTCTGGGGGCCCGGGACTTGATTTCCAGGCTTCTC
  AGATACCAGCCCTTGGAGAGACTGCCCCTGGCCCAGATCCTGAAGCACCCCTGGGTTCAG
  GCCCACTCCCGAAGGGTGCTGCCTCCCTGTGCTCAGATGGCTTCCTGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003160 unedited
  TATTTTGTAATACGACTCACTATAGGGCGGCNCGCGATTCGGCACGAGGCCAGAGCTGTG
  GTGCAGCTGGGCAAAGCTCAACCTGCAGGCGAAGAGTTGGCTACAGCAAACCAAACAGCC
  CAGCAGCCCAGCAGCCCAGCCATGCGGCGCCTCACAGTCGATGACTTTGAAATCGGGCGT
  CCCCTGGGCAAGGGGAAATTTGGGAATGTGTACCTGGCTCGGCTCAAGGAAAGCCATTTC
  ATTGTGGCCCTGAAGGTTCTCTTCAAGTCGCAGATAGAGAAGGAAGGACTGGAGCACCAG
  CTGCGCCGGGAAATTGAGATCCAGGCTCATCTACAACACCCCAATATCCTGCGCCTGTAT
  AACTATTTCCATGATGCACGCCGGGTGTACCTGATTCTGGAATATGCTCCAAGGGGTGAG
  CTCTACAAGGAGCTGCAGAAAAGCGAGAAATTAGATGAACAGCGCACAGCCACGATAATA
  GAGGAGTTGGCAGATGCCCTGACCTACTGCCATGACAAGAAAGTGATTCACAGAGATATT
  AAGCCAGAGAACCTGCTGCTGGGGTTCAGGGGTGAGGTGAAGATTGCAGATTTTGGCTGG
  TCTGTGCACACCCCCTCCCTGAGGAGGAAGACAATGTGTGGGACACTGGACTACTTGCCG
  CCAGAAATGATTGAGGNGAGAACATATGATGAAAAGGTGGATTTGTGGTGCATTGGAGTG
  CTCTGCTATGAGCTGCTGGTGGGATATCCACCCTTTGAGAGCGCCTCCCACAGTGAGACT
  TACAGACGCATNCTCCAGGTAGATGTGAGGTTTCCACTATCAATGCCTCTGGNGGGCCCG
  GGACTTGATTTCCAGCTTCTCAGAACCACCCCTTGAGAGACTGNCCCTGGCCCAGACCTG
  ACCCCCCTTGGGTTCAGGCCACTCCCGAAGGTGCTGCCTCCTGNGCTCAAATGCTCCTGA
  GCCTGTCTGCTCTGTTCCTTGGGGGGGT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003160 unedited
  NGGAATTCTTTGGACGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGCTGG
  TATGAAATGATTCAGCTTTTATTAATTAGCATCTTACATCTTAAAAGAGAAAAAAATAAA
  GACAAATGAGGTGGCAGAGCCAGGAGAGCTCCCTGAACACACACAAAGGGAACAGAGGCA
  GACAGGGCTCAGGAAGCCATCTGAGCACAGGGAGGCAGCACCCTTCGGGAGTGGGCCTGA
  ACCCAGGGGTGCTTCAGGATCTGGGCCAGGGGCAGTCTCTCCAAGGGCTGGTATCTGAGA
  AGCCTGGAAATCAAGTCCCGGGCCCCCAGAGGCATTGATAGTGGAAACCTCACATCTACC
  TTGAGGATGCGTCTGTAAGTCTCACTGTGGGAGGCGCTCTCAAAGGGTGGATATCCCACC
  AGCAGCTCATAGCAGAGCACTCCAATGCACCACAAATCCACCTTTTCATCATATGTTCTC
  CCCTCAATCATTTCTGGCGGCAAGTAGTCCAGTGTCCCACACATTGTCTTCCTCCTCAGG
  GAGGGGGTGTGCACAGACCAGCCAAAATCTGCAATCTTCACCTCACCCCTGAACCCCAGC
  AGCAGGTTCTCTGGCTTAATATCTCTGTGAATCACTTTCTTGTCATGGCAGTAGGTCAGG
  GCATCTGCCAACTCCTCTATTATCGTGGCTGTGCGCTGTTCATCTAATTTCTCGCTTTTC
  TGCAGCTCCTTGTAGAGCTCACCCCTTGGAGCATATTCCAGAATCAAGTACACCCCGGCG
  TGCATCATGGAAATAGTTATACAGGCGCAAGATATTGGGGTGTTGTAGATGAACCTGGAT
  CTCAATTTCCCGGCGCAGCTGGTGCTTCAGATCCTTCTTCTCTATCTGCGACTTGAAGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003160
• Insert Size: 1110 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003160.2, NP_003151.2
• RefSeq Size: 1125 bp
• RefSeq ORF: 828 bp
• Locus ID: 6795
• UniProt ID: Q9UQB9
• Cytogenetics: 19q13.43
• Domains: pkinase, TyrKc, S_TKc
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the Aurora subfamily of serine/threonine protein kinases. The encoded protein is a chromosomal passenger protein that forms complexes with Aurora-B and inner centromere proteins and may play a role in organizing microtubules in relation to centrosome/spindle function during mitosis. This gene is overexpressed in several cancer cell lines, suggesting an involvement in oncogenic signal transduction. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (3) uses an alternate splice site at the 5' end of the first intron and an alternate downstream translation initiation site, compared to variant 1. The encoded protein (isoform 3) has a shorter N-terminus, compared to isoform 1.