TBCE (NM_003193) Human Untagged Clone

CAT#: SC118123

TBCE (untagged)-Human tubulin folding cofactor E (TBCE), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TBCE
• Synonyms: HRD; KCS; KCS1; pac2; PEAMO
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC118123 sequence for NM_003193 edited (data generated by NextGen Sequencing)
  ATGAGTGACACTTTGACAGCGGATGTCATTGGTCGAAGAGTTGAAGTTAATGGAGAACAT
  GCAACAGTACGTTTTGCTGGTGTTGTCCCTCCCGTGGCAGGACCCTGGTTAGGAGTAGAA
  TGGGACAATCCCGAGAGAGGAAAGCATGATGGGAGCCACGAAGGGACTGTGTATTTTAAA
  TGCAGGCACCCGACAGGAGGATCCTTTATTCGTCCGAACAAGGTAAATTTTGGAACAGAC
  TTTCTTACTGCAATTAAGAACCGCTATGTGTTAGAAGATGGACCAGAGGAAGATAGAAAA
  GAGCAAATTGTTACAATTGGAAATAAACCTGTGGAGACTATCGGTTTTGACTCTATTATG
  AAACAGCAAAGTCAGCTGAGCAAGTTGCAAGAAGTTTCTCTGAGGAACTGTGCAGTAAGT
  TGTGCTGGTGAAAAAGGAGGAGTTGCTGAAGCATGTCCTAATATCAGAAAGGTAGATTTG
  TCAAAAAACCTGTTGTCATCATGGGATGAAGTGATACACATTGCTGATCAGCTCAGACAC
  CTGGAAGTCCTTAATGTCAGTGAAAATAAACTAAAATTTCCCTCCGGTTCAGTATTAACT
  GGAACGCTTTCTGTACTGAAGGTTTTAGTCCTCAATCAAACAGGAATAACGTGGGCTGAG
  GTGCTGCGGTGTGTCGCGGGGTGCCCAGGCCTGGAGGAACTCTACCTTGAGTCTAACAAC
  ATTTTCATTTCCGAAAGGCCAACAGATGTTCTCCAGACAGTCAAGTTATTAGATCTTTCC
  TCTAATCAATTAATTGATGAAAATCAGCTGTATCTGATAGCCCACCTGCCCAGGTTAGAA
  CAATTAATCCTCTCTGACACTGGAATTTCTTCTCTACATTTTCCGGATGCTGGAATTGGG
  TGCAAAACGTCCATGTTCCCATCCTTGAAGTACCTGGTAGTAAACGACAATCAGATATCA
  CAATGGTCGTTTTTCAATGAGCTAGAGAAGTTACCAAGTCTACGGGCTTTGTCCTGCCTA
  AGAAACCCCCTGACCAAAGAGGACAAAGAAGCAGAGACGGCGCGACTACTCATTATCGCC
  AGCATTGGCCAGCTGAAGACGCTGAACAAATGTGAGATTCTCCCCGAGGAGAGGCGGAGA
  GCTGAGCTTGACTACCGAAAAGCTTTTGGAAATGAGTGGAAACAGGCTGGTGGACATAAG
  GATCCGGAAAAAAACAGACTCAGCGAAGAATTCCTCACAGCCCATCCCAGATACCAGTTC
  CTCTGCCTGAAATATGGTGCACCTGAAGATTGGGAACTCAAAACACAGCAACCACTTATG
  CTGAAAAACCAGCTACTAACACTGAAGATAAAATACCCTCATCAACTTGATCAGAAAGTC
  CTGGAGAAACAACTGCCGGGCTCCATGACAATTCAAAAGGTGAAGGGATTGCTGTCACGT
  CTTCTCAAAGTTCCTGTGTCAGACCTTCTGTTGTCCTATGAAAGTCCCAAAAAGCCGGGC
  AGAGAAATCGAGCTGGAAAATGACCTAAAGTCATTACAGTTTTATTCTGTGGAAAATGGA
  GATTGTCTATTAGTGCGATGGTGA
  
  Clone variation with respect to NM_003193.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003193 unedited
  NGTTCAAATTTTCGTATACGACTCACTATAGGCGGCCCGCGAATTCGCACGAGGCCTCGT
  GCCGAATTCGGCACGAGGCTTCGCTGCTGGGCAGTTGGCTGGAGGGGCTGCTGCTGGGAA
  CACCTGGAGTCTCCGCGGGCAGATCTCATATTTTGGATTCTGGATATATTATAATGAGTG
  ACACTTTGACAGCGGATGTCATTGGTCGAAGAGTTGAAGTTAATGGAGAACATGCAACAG
  TACGTTTTGCTGGTGTTGTCCCTCCCGTGGCAGGACCCTGGTTAGGAGTAGAATGGGACA
  ATCCCGAGAGAGGAAAGCATGATGGGAGCCACGAAGGGACTGTGTATTTTAAATGCAGGC
  ACCCGACAGGAGGATCCTTTATTCGTCCGAACAAGGTAAATTTTGGAACAGACTTTCTTA
  CTGCAATTAAGAACCGCTATGTGTTAGAAGATGGACCAGAGGAAGATAGAAAAGAGCAAA
  TTGTTACAATTGGAAATAAACCTGTGGAGACTATCGGTTTTGACTCTATTATGAAACAGC
  AAAGTCAGCTGAGCAAGTTGCAAGAAGTTTCTCTGAGGAACTGTGCAGTAAGTTGTGCTG
  GTGAAAAAGGAGGAGGTGCTGAAGCATGTCCTAATATCAGAAAGGTAGATTTGTCAAAAA
  ACCTGTTGTCATCATGGGATGAAGTGATACACATTGCTGATCAGCTCAGACACCTGGAAG
  TCCTTAATGTCAGTGAAAATAAACTAAAAATTTCCCTCCGGNTCAGTATTAACTGGAACG
  CTTTCTGTACTGGAAGGTTTTAGTCCTCAATCAAACAGGAATAACGTTGGCTTGAGTGCT
  GCCGGTGTGTCCCCGGGGTGCCCAAGCCCTGGAGAACCCTACCCTTGAGTCTAACACCAT
  TTTCATTTCGAAAGGCCACAGATGTTCTC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003193 unedited
  ACCGCGGGCCGCAATCTANAGTCGAGTTTTTTTTTTTTTTTTTTAGGTTCAAAGCCTTTA
  TTATTGCTATTATGTATAAGCCTAGAATGGTCACTTCCCAACAAAAAATACATCCCTTGT
  TATACTTAGGACAAGTTGTAAACCCCCTTTGTTTTGACAGTAGAATTGTTCCAGTGAATC
  ATTTATTTCCGGTGAACCCCAGACACGATAAGCAGTGTGGTCTTTAAATTTTATTAGTTG
  GTTGTCACCATCGCACTAATAGACAATCTCCATTTTCCACAGAATAAAACTGTAATGACT
  TTAGGTCATTTTCCAGCTCGATTTCTCTGCCCGGCTTTTTGGGACTTTCATAGGACAACA
  GAAGGTCTGACACAGGAACTTTGAGAAGACGTGACAGCAATCCCTTCACCTTTTGAATTG
  TCATGGAGCCCGGCAGTTGTTTCTCCAGGACTTTCTGATCAAGTTGATGAGGGTATTTTA
  TCTTCAGTGTTAGTAGCTGGTTTTTCAGCATAAGTGGTTGCTGTGTTTTGAGTTCCCAAT
  CTTCAGGTGCACCATATTTCAGGCAGAGGAACTGGTATCTGGGATGGGCTGTGAGGAATT
  CTTCGCTGAGTCTGTTTTTTTCCGGATCCTTATGTCCACCAGCCTGTTTCCACTCATTTC
  CAAAAGCTTTTCGGTAGTCAAGCTCAGCTCTCCGCCTCTTCTCGGGGAGAATCTCACATT
  TGTTCAGCGTCTTCAGCTGGCCAATGCTGGCGATAATGAGTAGTCGCGCCGTCTCTGCTC
  TTTGTCCTCTTTGTCAGGGGGTTCTTAAGCAGGACAAACCCGTAACTTGTTACTTCTCTA
  CTCATTGAAAACGACCTCGNGAATTTGATGTCGTTACTACANGACTTCAGGATGGAACTG
  GCGTTTGCACCAATTCAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003193
• Insert Size: 2050 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003193.2, NP_003184.1
• RefSeq Size: 1882 bp
• RefSeq ORF: 1584 bp
• Locus ID: 6905
• UniProt ID: Q15813
• Cytogenetics: 1q42.3
• Domains: CAP_GLY, LRR
• Protein Families: Druggable Genome
• Gene Summary: Cofactor E is one of four proteins (cofactors A, D, E, and C) involved in the pathway leading to correctly folded beta-tubulin from folding intermediates. Cofactors A and D are believed to play a role in capturing and stabilizing beta-tubulin intermediates in a quasi-native confirmation. Cofactor E binds to the cofactor D/beta-tubulin complex; interaction with cofactor C then causes the release of beta-tubulin polypeptides that are committed to the native state. Two transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) differs in the 5' UTR, compared to variant 1. Both variants 1 and 2 encode the same isoform (a).