NFATC4 (NM_004554) Human Untagged Clone

CAT#: SC117276

NFATC4 (untagged)-Human nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 4 (NFATC4), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: NFATC4
• Synonyms: NF-AT3; NF-ATC4; NFAT3
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117276 sequence for NM_004554 edited (data generated by NextGen Sequencing)
  
  
  ATGGGGGCGGCCAGCTGCGAGGATGAGGAGCTGGAATTTAAGCTGGTGTTCGGGGAGGAAAAGGAGGCCC
  CCCCGCTGGGCGCGGGGGGATTGGGGGAAGAACTGGACTCAGAGGATGCCCCGCCATGCTGCCGTCTGGC
  CTTGGGAGAGCCCCCTCCCTATGGCGCTGCACCTATCGGTATTCCCCGACCTCCACCCCCTCGGCCTGGC
  ATGCATTCGCCACCGCCGCGACCAGCCCCCTCACCTGGCACCTGGGAGAGCCAGCCCGCCAGGTCGGTGA
  GGCTGGGAGGACCAGGAGGGGGTGCTGGGGGTGCTGGGGGTGGCCGTGTTCTCGAGTGTCCCAGCATCCG
  CATCACCTCCATCTCTCCCACGCCGGAGCCGCCAGCAGCGCTGGAGGACAACCCTGATGCCTGGGGGGAC
  GGCTCTCCTAGAGATTACCCCCCACCAGAAGGCTTTGGGGGCTACAGAGAAGCAGGGGGCCAGGGTGGGG
  GGGCCTTCTTCAGCCCAAGCCCTGGCAGCAGCAGCCTGTCCTCGTGGAGCTTCTTCTCCGATGCCTCTGA
  TGAGGCAGCCCTGTATGCAGCCTGCGACGAGGTGGAGTCTGAGCTAAATGAGGCGGCCTCCCGCTTTGGC
  CTGGGCTCCCCGCTGCCCTCGCCCCGGGCCTCCCCTCGGCCATGGACCCCCGAAGATCCCTGGAGCCTGT
  ATGGTCCAAGCCCCGGAGGCCGAGGGCCAGAGGATAGCTGGCTACTCCTCAGTGCTCCTGGGCCCACCCC
  AGCCTCCCCGCGGCCTGCCTCTCCATGTGGCAAGCGGCGCTATTCCAGCTCGGGAACCCCATCTTCAGCC
  TCCCCAGCTCTGTCCCGCCGTGGCAGCCTGGGGGAAGAGGGGTCTGAGCCACCTCCACCACCCCCATTGC
  CTCTGGCCCGGGACCCGGGCTCCCCTGGTCCCTTTGACTATGTGGGGGCCCCACCAGCTGAGAGCATCCC
  TCAGAAGACACGGCGGACTTCCAGCGAGCAGGCAGTGGCTCTGCCTCGGTCTGAGGAGCCTGCCTCATGC
  AATGGGAAGCTGCCCTTGGGAGCAGAGGAGTCTGTGGCTCCTCCAGGAGGTTCCCGGAAGGAGGTGGCTG
  GCATGGACTACCTGGCAGTGCCCTCCCCACTCGCTTGGTCCAAGGCCCGGATTGGGGGACACAGCCCTAT
  CTTCAGGACCTCTGCCCTACCCCCACTGGACTGGCCTCTGCCCAGCCAATATGAGCAGCTGGAGCTGAGG
  ATCGAGGTACAGCCTAGAGCCCACCACCGGGCCCACTATGAGACAGAAGGCAGCCGTGGAGCTGTCAAAG
  CTGCCCCTGGCGGTCACCCCGTAGTCAAGCTCCTAGGCTACAGTGAGAAGCCACTGACCCTACAGATGTT
  CATCGGCACTGCAGATGAAAGGAACCTGCGGCCTCATGCCTTCTATCAGGTGCACCGTATCACAGGCAAG
  ATGGTGGCCACGGCCAGCTATGAAGCCGTAGTCAGTGGCACCAAGGTGTTGGAGATGACTCTGCTGCCTG
  AGAACAACATGGCGGCCAACATTGACTGCGCGGGAATCCTGAAGCTTCGGAATTCAGACATTGAGCTTCG
  GAAGGGTGAGACGGACATCGGGCGCAAAAACACACGTGTACGGCTGGTGTTCCGGGTACACGTGCCCCAG
  GGCGGCGGGAAGGTCGTCTCAGTACAGGCAGCATCGGTGCCCATCGAGTGCTCCCAGCGCTCAGCCCAGG
  AGCTGCCCCAGGTGGAGGCCTACAGCCCCAGTGCCTGCTCTGTGAGAGGAGGCGAGGAACTGGTACTGAC
  TGGCTCCAACTTCCTGCCAGACTCCAAGGTGGTGTTCATTGAGAGGGGTCCTGATGGGAAGCTGCAATGG
  GAGGAGGAGGCCACAGTGAACCGACTGCAGAGCAACGAGGTGACGCTGACCCTGACTGTCCCCGAGTACA
  GCAACAAGAGGGTTTCCCGGCCAGTCCAGGTCTACTTTTATGTCTCCAATGGGCGGAGGAAACGCAGTCC
  TACCCAGAGTTTCAGGTTTCTGCCTGTGATCTGCAAAGAGGAGCCCCTACCGGACTCATCTCTGCGGGGT
  TTCCCTTCAGCATCGGCAACCCCCTTTGGCACTGACATGGACTTCTCACCACCCAGGCCCCCCTACCCCT
  CCTATCCCCATGAAGACCCTGCTTGCGAAACTCCTTACCTATCAGAAGGCTTCGGCTATGGCATGCCCCC
  TCTGTACCCCCAGACGGGGCCCCCACCATCCTACAGACCGGGCCTGCGGATGTTCCCTGAGACTAGGGGT
  ACCACAGGTTGTGCCCAACCACCTGCAGTTTCCTTCCTTCCCCGCCCCTTCCCTAGTGACCCGTATGGAG
  GGCGGGGCTCCTCTTTCTCCCTGGGGCTGCCATTCTCTCCGCCAGCCCCCTTTCGGCCGCCTCCTCTTCC
  TGCATCCCCACCGCTTGAAGGCCCCTTCCCTTCCCAGAGTGATGTGCATCCCCTACCTGCTGAGGGATAC
  AATAAGGTAGGGCCAGGCTATGGCCCTGGGGAGGGGGCTCCGGAGCAGGAGAAATCCAGGGGTGGCTACA
  GCAGCGGCTTCCGAGACAGTGTCCCTATCCAGGGTATCACGCTGGAGGAAGTGAGTGAGATCATTGGCCG
  AGACCTGAGTGGCTTCCCTGCACCTCCTGGAGAAGAGCCTCCTGCCTGA
  
  Clone variation with respect to NM_004554.4
  561:t=>c
• Restriction Sites: Please inquire
• ACCN: NM_004554
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: The ORF of this clone has been fully sequenced and found to be a perfect match to NM_004554.3.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004554.3, NP_004545.2
• RefSeq Size: 4976 bp
• RefSeq ORF: 2709 bp
• Locus ID: 4776
• UniProt ID: Q14934
• Cytogenetics: 14q12
• Domains: IPT
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Axon guidance, B cell receptor signaling pathway, MAPK signaling pathway, Natural killer cell mediated cytotoxicity, T cell receptor signaling pathway, VEGF signaling pathway, Wnt signaling pathway
• Gene Summary: This gene encodes a member of the nuclear factor of activated T cells (NFAT) protein family. The encoded protein is part of a DNA-binding transcription complex. This complex consists of at least two components: a preexisting cytosolic component that translocates to the nucleus upon T cell receptor stimulation and an inducible nuclear component. NFAT proteins are activated by the calmodulin-dependent phosphatase, calcineurin. The encoded protein plays a role in the inducible expression of cytokine genes in T cells, especially in the induction of interleukin-2 and interleukin-4. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2014]
  Transcript Variant: This variant (2) is alternatively spliced at the 5' and 3' ends compared to transcript variant 1. This results in the use of an in-frame downstream AUG, translation termination at a downstream stop codon, and a shorter isoform (2) with a distinct C-terminus compared to isoform 1.