Peregrin (BRPF1) (NM_004634) Human Untagged Clone

CAT#: SC117237

BRPF1 (untagged)-Human bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Peregrin
• Synonyms: BR140; IDDDFP
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC117237 sequence for NM_004634 edited (data generated by NextGen Sequencing)
  ATGGGGGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCA
  TACGAGTGCCCGGTGGAGACCTGCCGAAAGGTCTACAAGAGTTACAGTGGTATTGAGTAC
  CACCTGTACCACTATGACCACGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAG
  CACAAGAAAAAGGGGCGCCAGTCACGCCCAGCCAACAAGCAGTCACCCAGCCCCTCAGAG
  GTCTCACAGTCACCAGGCCGTGAGGTGATGAGCTATGCACAGGCCCAGCGCATGGTGGAG
  GTGGACTTGCATGGCCGCGTCCACCGCATCAGCATCTTTGACAACCTGGATGTGGTGTCA
  GAGGATGAGGAAGCCCCCGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAGAACACTGAG
  ACACCAGCTGCTACTCCCAAGTCAGGCAAACATAAGAACAAGGAGAAGCGCAAGGACTCC
  AACCATCACCACCACCACAATGTTTCTGCGAGCACCACTCCCAAGCTGCCAGAGGTGGTC
  TATCGGGAGCTGGAACAGGACACCCCTGATGCCCCACCCCGGCCAACTTCCTATTACCGG
  TACATCGAGAAGTCTGCAGAGGAGCTGGACGAGGAAGTAGAGTATGACATGGACGAGGAG
  GACTACATCTGGCTGGATATCATGAATGAGCGTCGGAAGACAGAGGGTGTAAGTCCCATC
  CCGCAGGAGATCTTTGAGTACCTAATGGACCGACTGGAGAAGGAGTCGTACTTTGAGAGT
  CATAATAAAGGCGACCCTAATGCGCTAGTGGACGAGGATGCTGTTTGCTGTATCTGCAAT
  GATGGTGAGTGCCAGAACAGCAATGTCATCCTCTTCTGTGACATGTGCAACCTGGCCGTG
  CACCAGGAGTGCTACGGTGTCCCCTATATCCCTGAGGGCCAGTGGCTGTGCCGCCGTTGC
  CTGCAGTCACCCTCTCGTGCTGTGGATTGTGCCCTGTGCCCCAACAAGGGCGGTGCCTTC
  AAGCAGACAGATGACGGGCGCTGGGCCCATGTGGTGTGTGCCTTGTGGATCCCTGAGGTC
  TGCTTCGCCAACACGGTCTTCCTAGAGCCTATTGACAGCATTGAGCACATCCCACCAGCT
  CGCTGGAAGCTCACCTGCTACATTTGCAAACAACGGGGCTCAGGGGCCTGCATCCAGTGC
  CACAAGGCCAACTGTTACACAGCTTTCCATGTGACATGCGCCCAGCAGGCTGGCCTTTAC
  ATGAAGATGGAGCCTGTGCGGGAGACAGGCGCCAACGGCACCTCTTTCAGTGTCCGCAAG
  ACAGCCTACTGCGACATCCACACGCCTCCAGGTTCAGCACGCCGACTGCCTGCCCTGTCC
  CACAGCGAGGGTGAGGAGGATGAAGATGAGGAGGAGGATGAGGGTAAGGGCTGGAGCTCA
  GAGAAAGTCAAGAAGGCCAAGGCCAAGTCCCGGATCAAAATGAAGAAGGCACGGAAGATC
  CTGGCAGAGAAGCGGGCAGCAGCACCTGTGGTGTCAGTGCCCTGCATCCCACCACACAGG
  CTTAGTAAAATCACCAACCGCCTGACCATCCAAAGGAAGAGCCAGTTCATGCAGAGGCTG
  CACAGCTACTGGACACTGAAGCGGCAGTCACGGAATGGGGTCCCATTGCTACGTCGCCTG
  CAGACACACCTGCAATCTCAGAGGAACTGTGACCAAGTTGGGAGAGATTCTGAGGATAAG
  AACTGGGCCCTTAAAGAACAGCTCAAGTCCTGGCAGCGGCTCCGGCATGACTTGGAGCGA
  GCTCGGCTGCTCGTGGAATTGATCCGCAAGCGGGAAAAACTCAAAAGGGAGACGATCAAG
  GTTCAGCAGATTGCCATGGAGATGCAGCTGACTCCTTTCCTCATCCTCCTTCGCAAAACC
  TTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCAGCGAGCCGGTCCCTCTGTCT
  GAGGTACCTGACTACCTAGACCACATCAAAAAGCCCATGGACTTTTTCACCATGAAGCAG
  AACTTGGAGGCTTACCGCTACCTGAATTTTGATGATTTTGAGGAGGACTTCAACCTCATC
  GTCAGCAACTGCCTCAAGTATAACGCCAAGGACACCATCTTCTACCGGGCAGCAGTGCGG
  CTTCGTGAGCAGGGTGGTGCTGTGCTCCGCCAGGCCCGGCGCCAGGCAGAAAAAATGGGC
  ATTGACTTTGAGACGGGCATGCATATCCCCCACAGCCTGGCTGGAGATGAGGCCACACAC
  CACACTGAAGATNNNNNCGAGGAAGAGCGGCTGGTCTTGCTGGAGAACCAGAAGCACCTG
  CCAGTGGAAGAACAGCTAAAGCTGCTTCTGGAGCGGCTGGACGAAGTGAATGCCAGCAAG
  CAGAGTGTGGGCCGCTCACGGCGTGCAAAGATGATCAAGAAAGAGATGACGGCACTGCGG
  CGGAAGCTTGCCCATCAGCGAGAGACGGGACGTGATGGCCCTGAGCGGCATGGCCCCTCG
  AGCCGGGGTAGTCTGACACCCCACCCGGCAGCCTGTGACAAGGATGGGCAGACAGATAGT
  GCGGCAGAGGAGAGCAGCAGCCAGGAGACAAGCAAAGGCCTGGGTCCCAACATGTCCTCA
  ACCCCCGCACATGAGGTGGGCAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAG
  ACAGCTGGACCGCCCAAGAGGCCGGGCCGGCCCCCCAAAAACCGGGAGAGCCAGATGACC
  CCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCCCAGCTCCCCATCATGAGTTCCCTGCGT
  CAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCGAGCTCAGACAGCGACAGTGATAAG
  TCCACAGAAGACCCCCCAATGGACTTACCAGCCAATGGCTTCAGCGGTGGAAACCAACCA
  GTGAAGAAGAGTTTCTTGGTATACCGTAATGACTGCAGCCTTCCCCGGAGCAGCTCAGAC
  TCTGAGTCCAGCAGCAGTAGCAGTAGCAGCGCTGCTTCAGACCGGACCAGCACAACGCCC
  TCAAAACAAGGCCGGGGCAAACCCTCCTTCTCTCGGGGCACTTTCCCAGAGGACAGCAGT
  GAGGATACCTCAGGCACTGAGAATGAGGCCTACTCCGTGGGCACTGGCCGCGGCGTGGGC
  CACAGCATGGTAAGGAAGAGTCTGGGCCGGGGAGCTGGCTGGCTGTCAGAGGATGAGGAC
  TCCCCGCTGGATGCTCTGGACCTCGTGTGGGCCAAATGCCGAGGCTATCCATCATACCCA
  GCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCATGGGGTTCCCATC
  CCTGTGCCCCCACTGGAGGTGCTGAAACTTGGGGAGCAGATGACCCAGGAAGCCCGAGAG
  CATCTCTACCTCGTCCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCAGGACC
  AAGCTGGTTCCTCTGGGTGTGAACCAGGACCTAGACAAGGAGAAGATGCTGGAGGGCCGC
  AAGTCCAACATCCGCAAGTCAGTACAGATCGCCTACCACAGGGCTCTGCAGCACCGCAGC
  AAGGTGCAAGGCGAGCAGAGCAGTGAGACCAGCGATAGTGATTGA
  
  Clone variation with respect to NM_004634.2
  2293 g=>n;2294 c=>n;2295 a=>n;2296 g=>n;2297 c=>n
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_004634 unedited
  CCATTAGTATACGACTCACTATAGGCGGCCGCGCAATTCGCACGAGGCGGAATCCCGGGG
  AGCCGGGACCAAAGCGTGGGGCGGCAGGGGGCCGGGGTGGCGAGGTCTACGGTCTCCGGA
  GTTGGGGCTCCCCCTTCCCGGCCCGACTTTCCCCGCCGACCTCCCCAAGTGGGGTCGGAG
  CCCCGGCGGGCGCCCCCGGCGATGAGCCCGGACTCGAGGTGGCCGACGTGACAGCATGGG
  GGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCATACGA
  GTGCCCGGTGGAGACCTGCCGAAAGGTCTACAAGAGTTACAGTGGTATTGAGTACCACCT
  GTACCACTATGACCACGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAGCACAA
  GAAAAAGGGGCGCCAGTCACGCCCAGCCAACAAGCAGTCACCCAGCCCCTCAGAGGTCTC
  ACAGTCACCAGGCCGTGAGGTGATGAGCTATGCACAGGCCCAGCGCATGGTGGAGGTGGA
  CTTGCATGGCCGCGTCCACCGCATCAGCCTCTTTTGACCACCCTGGATGTGGTGTCACAC
  GATGAGGAAGCCCCCCGAGGAGGCCCCTGAGAATGGCAGCCACAAGGCAGAACACTGAGA
  CACCAGTTGCTACTCTCAAGTCACGCCAACCTAACAACAAGGAGAAGCGCAGGGATTCAA
  CCCTCACCACCACCCCATGTTTTTGCGAACACCACTCCCAGCTGCCAAAGGGGGCTATCG
  GGAGCCGAACCAGACACCCTGGTGCCCCACCCCGTCAACTCCTTATAACCGGTCATCTAA
  AATCTCGCAAGAGCCGGACCAGGAATCCAGTTGACTTGACCAAGGCGCCCACTCCGGCCG
  CAATACGAAAGAACCGTCGAAGAATAGGGGTAAATCCCTACCCCACAGATCTTGAGTCCC
  TAAGGACCN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_004634 unedited
  TGTAACGACGGCCCGCATTCTAGTAGTCGAGTTTTTTTTTTTTTTTTTTTTTAGTACCAA
  ATGACTCTGGCGCGACCCGGAAACGCAGCTACAAATGAGACATAATTTAAATTCTCCGAT
  AATTACAGTATTTACAACAGCAGGGGAGGGAGTCACCTATTGCCCCTCTTCCAGGCTGGA
  CAGATGCCCACTCACCTCACGACCGGTAGGGGCCCCAGGCCTCTAGGCAGTTATGGATTC
  TCCTGGCATGAGCAGGTAGAGGTGGTGGGATGGCCTGTTTTACGCAGTGTCCTAACTTTG
  GCCCACCACCCCGCCCTACAATGCTGTGAGGTCTCTGGAGCTTTTTCTGACCTGTGGGCC
  CTCCCTCTCCTTGCGCTGAACTGCCATTGGCCCTTGGAGTGTTCTGTACAGAACAGCTGT
  ACTTATGGCAGGAGGGACCTCCCCCACCCAGCTTCCCCTGAGACTGGCCCCAAGACCATA
  AATGAGTCAGTGCACAGGCCGGTGCCACCTCCAGGACAGCGAGCACAGGGGAGGAGAGAA
  GCCACAGGGCACTATAGGCTGGGCTGTGTTGAACATTATCAACACTATCGCTGGTCCTAC
  TGCTCTGCACGCCCTGAACCTTGATGCGGTGCTGAATAGCCCTGTGGTATGCGAACTGAT
  CTGACTTGCGGACGTTGAACTTGCGGTCTCCAACAATTTTCTCTTTGTTAGGATCTGGTT
  CCACTAGAAGAACCAATTTGCTCCTGGGAACCCTGCTAAAACCGCTGGTGCACATAATAG
  GCACAGGATCAACGCTATCGGCTTTTCTGGGTCCCCGCTACCCAACTATCCTACTTCTCG
  GGGGCTCATGAATGCCACCTTCTGGTGGACATCACTCGCGCGCCTTCTTCGATATAGCTC
  CATACGTGGATCTAGACATACTAGATCTTGTCTCCTGACAGCTCAGANTACACCGCGGAT
  ACTAAACCATGCCACTAACG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_004634
• Insert Size: 4540 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004634.2, NP_004625.2
• RefSeq Size: 4710 bp
• RefSeq ORF: 3645 bp
• Locus ID: 7862
• UniProt ID: P55201
• Cytogenetics: 3p25.3
• Domains: PWWP, BROMO, PHD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a bromodomain, PHD finger and chromo/Tudor-related Pro-Trp-Trp-Pro (PWWP) domain containing protein. The encoded protein is a component of the MOZ/MORF histone acetyltransferase complexes which function as a transcriptional regulators. This protein binds to the catalytic MYST domains of the MOZ and MORF proteins and may play a role in stimulating acetyltransferase and transcriptional activity of the complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (2) lacks an in-frame segment in the coding region, as compared to variant 1. The encoded isoform 2 is thus shorter than isoform 1.