Peregrin (BRPF1) (NM_004634) Human Tagged ORF Clone

CAT#: RG214561

BRPF1 (tGFP-tagged) - Human bromodomain and PHD finger containing, 1 (BRPF1), transcript variant 2

Product Images

Circular map for RG214561

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: Peregrin
• Synonyms: BR140; IDDDFP
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214561 representing NM_004634
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGTGGACTTTGATGTGAAGACTTTCTGCCACAACTTGCGGGCGACTAAGCCACCATACGAGTGCC
  CGGTGGAGACCTGCCGAAAGGTCTACAAGAGTTACAGTGGTATTGAGTACCACCTGTACCACTATGACCA
  CGACAACCCACCACCCCCACAACAAACTCCACTCCGCAAGCACAAGAAAAAGGGGCGCCAGTCACGCCCA
  GCCAACAAGCAGTCACCCAGCCCCTCAGAGGTCTCACAGTCACCAGGCCGTGAGGTGATGAGCTATGCAC
  AGGCCCAGCGCATGGTGGAGGTGGACTTGCATGGCCGCGTCCACCGCATCAGCATCTTTGACAACCTGGA
  TGTGGTGTCAGAGGATGAGGAAGCCCCCGAGGAGGCCCCTGAGAATGGCAGCAACAAGGAGAACACTGAG
  ACACCAGCTGCTACTCCCAAGTCAGGCAAACATAAGAACAAGGAGAAGCGCAAGGACTCCAACCATCACC
  ACCACCACAATGTTTCTGCGAGCACCACTCCCAAGCTGCCAGAGGTGGTCTATCGGGAGCTGGAACAGGA
  CACCCCTGATGCCCCACCCCGGCCAACTTCCTATTACCGGTACATCGAGAAGTCTGCAGAGGAGCTGGAC
  GAGGAAGTAGAGTATGACATGGACGAGGAGGACTACATCTGGCTGGATATCATGAATGAGCGTCGGAAGA
  CAGAGGGTGTAAGTCCCATCCCGCAGGAGATCTTTGAGTACCTAATGGACCGACTGGAGAAGGAGTCGTA
  CTTTGAGAGTCATAATAAAGGCGACCCTAATGCGCTAGTGGACGAGGATGCTGTTTGCTGTATCTGCAAT
  GATGGTGAGTGCCAGAACAGCAATGTCATCCTCTTCTGTGACATGTGCAACCTGGCCGTGCACCAGGAGT
  GCTACGGTGTCCCCTATATCCCTGAGGGCCAGTGGCTGTGCCGCCGTTGCCTGCAGTCACCCTCTCGTGC
  TGTGGATTGTGCCCTGTGCCCCAACAAGGGCGGTGCCTTCAAGCAGACAGATGACGGGCGCTGGGCCCAT
  GTGGTGTGTGCCTTGTGGATCCCTGAGGTCTGCTTCGCCAACACGGTCTTCCTAGAGCCTATTGACAGCA
  TTGAGCACATCCCACCAGCTCGCTGGAAGCTCACCTGCTACATTTGCAAACAACGGGGCTCAGGGGCCTG
  CATCCAGTGCCACAAGGCCAACTGTTACACAGCTTTCCATGTGACATGCGCCCAGCAGGCTGGCCTTTAC
  ATGAAGATGGAGCCTGTGCGGGAGACAGGCGCCAACGGCACCTCTTTCAGTGTCCGCAAGACAGCCTACT
  GCGACATCCACACGCCTCCAGGTTCAGCACGCCGACTGCCTGCCCTGTCCCACAGCGAGGGTGAGGAGGA
  TGAAGATGAGGAGGAGGATGAGGGTAAGGGCTGGAGCTCAGAGAAAGTCAAGAAGGCCAAGGCCAAGTCC
  CGGATCAAAATGAAGAAGGCACGGAAGATCCTGGCAGAGAAGCGGGCAGCAGCACCTGTGGTGTCAGTGC
  CCTGCATCCCACCACACAGGCTTAGTAAAATCACCAACCGCCTGACCATCCAAAGGAAGAGCCAGTTCAT
  GCAGAGGCTGCACAGCTACTGGACACTGAAGCGGCAGTCACGGAATGGGGTCCCATTGCTACGTCGCCTG
  CAGACACACCTGCAATCTCAGAGGAACTGTGACCAAGTTGGGAGAGATTCTGAGGATAAGAACTGGGCCC
  TTAAAGAACAGCTCAAGTCCTGGCAGCGGCTCCGGCATGACTTGGAGCGAGCTCGGCTGCTCGTGGAATT
  GATCCGCAAGCGGGAAAAACTCAAAAGGGAGACGATCAAGGTTCAGCAGATTGCCATGGAGATGCAGCTG
  ACTCCTTTCCTCATCCTCCTTCGCAAAACCTTGGAGCAGCTCCAAGAGAAGGACACAGGCAACATCTTCA
  GCGAGCCGGTCCCTCTGTCTGAGGTACCTGACTACCTAGACCACATCAAAAAGCCCATGGACTTTTTCAC
  CATGAAGCAGAACTTGGAGGCTTACCGCTACCTGAATTTTGATGATTTTGAGGAGGACTTCAACCTCATC
  GTCAGCAACTGCCTCAAGTATAACGCCAAGGACACCATCTTCTACCGGGCAGCAGTGCGGCTTCGTGAGC
  AGGGTGGTGCTGTGCTCCGCCAGGCCCGGCGCCAGGCAGAAAAAATGGGCATTGACTTTGAGACGGGCAT
  GCATATCCCCCACAGCCTGGCTGGAGATGAGGCCACACACCACACTGAAGATGCAGCCGAGGAAGAGCGG
  CTGGTCTTGCTGGAGAACCAGAAGCACCTGCCAGTGGAAGAACAGCTAAAGCTGCTTCTGGAGCGGCTGG
  ACGAAGTGAATGCCAGCAAGCAGAGTGTGGGCCGCTCACGGCGTGCAAAGATGATCAAGAAAGAGATGAC
  GGCACTGCGGCGGAAGCTTGCCCATCAGCGAGAGACGGGACGTGATGGCCCTGAGCGGCATGGCCCCTCG
  AGCCGGGGTAGTCTGACACCCCACCCGGCAGCCTGTGACAAGGATGGGCAGACAGATAGTGCGGCAGAGG
  AGAGCAGCAGCCAGGAGACAAGCAAAGGCCTGGGTCCCAACATGTCCTCAACCCCCGCACATGAGGTGGG
  CAGGAGAACCTCAGTTCTGTTCTCCAAAAAGAACCCGAAGACAGCTGGACCGCCCAAGAGGCCGGGCCGG
  CCCCCCAAAAACCGGGAGAGCCAGATGACCCCCAGCCACGGAGGCAGTCCTGTGGGGCCCCCCCAGCTCC
  CCATCATGAGTTCCCTGCGTCAGCGCAAGCGGGGTAGGAGCCCCCGGCCCAGTTCGAGCTCAGACAGCGA
  CAGTGATAAGTCCACAGAAGACCCCCCAATGGACTTACCAGCCAATGGCTTCAGCGGTGGAAACCAACCA
  GTGAAGAAGAGTTTCTTGGTATACCGTAATGACTGCAGCCTTCCCCGGAGCAGCTCAGACTCTGAGTCCA
  GCAGCAGTAGCAGTAGCAGCGCTGCTTCAGACCGGACCAGCACAACGCCCTCAAAACAAGGCCGGGGCAA
  ACCCTCCTTCTCTCGGGGCACTTTCCCAGAGGACAGCAGTGAGGATACCTCAGGCACTGAGAATGAGGCC
  TACTCCGTGGGCACTGGCCGCGGCGTGGGCCACAGCATGGTAAGGAAGAGTCTGGGCCGGGGAGCTGGCT
  GGCTGTCAGAGGATGAGGACTCCCCGCTGGATGCTCTGGACCTCGTGTGGGCCAAATGCCGAGGCTATCC
  ATCATACCCAGCTCTGATCATTGATCCAAAGATGCCCCGAGAAGGTATGTTCCACCATGGGGTTCCCATC
  CCTGTGCCCCCACTGGAGGTGCTGAAACTTGGGGAGCAGATGACCCAGGAAGCCCGAGAGCATCTCTACC
  TCGTCCTCTTCTTTGACAACAAACGAACCTGGCAGTGGCTGCCCAGGACCAAGCTGGTTCCTCTGGGTGT
  GAACCAGGACCTAGACAAGGAGAAGATGCTGGAGGGCCGCAAGTCCAACATCCGCAAGTCAGTACAGATC
  GCCTACCACAGGGCTCTGCAGCACCGCAGCAAGGTGCAAGGCGAGCAGAGCAGTGAGACCAGCGATAGTG
  AT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214561 representing NM_004634
  Red=Cloning site Green=Tags(s)
  
  MGVDFDVKTFCHNLRATKPPYECPVETCRKVYKSYSGIEYHLYHYDHDNPPPPQQTPLRKHKKKGRQSRP
  ANKQSPSPSEVSQSPGREVMSYAQAQRMVEVDLHGRVHRISIFDNLDVVSEDEEAPEEAPENGSNKENTE
  TPAATPKSGKHKNKEKRKDSNHHHHHNVSASTTPKLPEVVYRELEQDTPDAPPRPTSYYRYIEKSAEELD
  EEVEYDMDEEDYIWLDIMNERRKTEGVSPIPQEIFEYLMDRLEKESYFESHNKGDPNALVDEDAVCCICN
  DGECQNSNVILFCDMCNLAVHQECYGVPYIPEGQWLCRRCLQSPSRAVDCALCPNKGGAFKQTDDGRWAH
  VVCALWIPEVCFANTVFLEPIDSIEHIPPARWKLTCYICKQRGSGACIQCHKANCYTAFHVTCAQQAGLY
  MKMEPVRETGANGTSFSVRKTAYCDIHTPPGSARRLPALSHSEGEEDEDEEEDEGKGWSSEKVKKAKAKS
  RIKMKKARKILAEKRAAAPVVSVPCIPPHRLSKITNRLTIQRKSQFMQRLHSYWTLKRQSRNGVPLLRRL
  QTHLQSQRNCDQVGRDSEDKNWALKEQLKSWQRLRHDLERARLLVELIRKREKLKRETIKVQQIAMEMQL
  TPFLILLRKTLEQLQEKDTGNIFSEPVPLSEVPDYLDHIKKPMDFFTMKQNLEAYRYLNFDDFEEDFNLI
  VSNCLKYNAKDTIFYRAAVRLREQGGAVLRQARRQAEKMGIDFETGMHIPHSLAGDEATHHTEDAAEEER
  LVLLENQKHLPVEEQLKLLLERLDEVNASKQSVGRSRRAKMIKKEMTALRRKLAHQRETGRDGPERHGPS
  SRGSLTPHPAACDKDGQTDSAAEESSSQETSKGLGPNMSSTPAHEVGRRTSVLFSKKNPKTAGPPKRPGR
  PPKNRESQMTPSHGGSPVGPPQLPIMSSLRQRKRGRSPRPSSSSDSDSDKSTEDPPMDLPANGFSGGNQP
  VKKSFLVYRNDCSLPRSSSDSESSSSSSSSAASDRTSTTPSKQGRGKPSFSRGTFPEDSSEDTSGTENEA
  YSVGTGRGVGHSMVRKSLGRGAGWLSEDEDSPLDALDLVWAKCRGYPSYPALIIDPKMPREGMFHHGVPI
  PVPPLEVLKLGEQMTQEAREHLYLVLFFDNKRTWQWLPRTKLVPLGVNQDLDKEKMLEGRKSNIRKSVQI
  AYHRALQHRSKVQGEQSSETSDSD
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004634
• ORF Size: 3642 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004634.2, NP_004625.2
• RefSeq Size: 4710 bp
• RefSeq ORF: 3645 bp
• Locus ID: 7862
• UniProt ID: P55201
• Cytogenetics: 3p25.3
• Domains: PWWP, BROMO, PHD
• Protein Families: Druggable Genome, Transcription Factors
• Gene Summary: This gene encodes a bromodomain, PHD finger and chromo/Tudor-related Pro-Trp-Trp-Pro (PWWP) domain containing protein. The encoded protein is a component of the MOZ/MORF histone acetyltransferase complexes which function as a transcriptional regulators. This protein binds to the catalytic MYST domains of the MOZ and MORF proteins and may play a role in stimulating acetyltransferase and transcriptional activity of the complex. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]