FARP1 (NM_005766) Human Untagged Clone

CAT#: SC116517

FARP1 (untagged)-Human FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived) (FARP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: FARP1
• Synonyms: CDEP; FARP1-IT1; PLEKHC2; PPP1R75
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC116517 sequence for NM_005766 edited (data generated by NextGen Sequencing)
  ATGGGAGAAATAGAGCAGAGGCCGACCCCAGGATCACGACTGGGGGCCCCGGAAAATTCG
  GGGATCAGTACCTTGGAACGTGGACAGAAGCCGCCCCCAACACCTTCAGGAAAACTCGTG
  TCCATCAAAATCCAGATGCTGGATGACACCCAGGAGGCATTTGAAGTTCCACAAAGAGCT
  CCTGGGAAGGTGCTGCTGGATGCAGTTTGCAACCACCTCAACCTCGTGGAAGGTGACTAT
  TTTGGCCTCGAGTTTCCTGATCACAAAAAGATCACGGTGTGGCTGGATCTCCTAAAACCC
  ATTGTGAAACAGATTAGAAGGCCAAAGCACGTTGTTGTTAAGTTTGTGGTGAAATTCTTT
  CCGCCTGACCACACACAACTCCAAGAAGAACTCACAAGGTACCTGTTCGCGCTGCAGGTG
  AAGCAGGACTTGGCTCAAGGCAGGTTGACGTGTAATGACACCAGCGCAGCTCTCTTGATT
  TCACACATTGTGCAATCTGAGATTGGGGATTTTGATGAAGCCTTGGACAGAGAGCACTTA
  GCAAAAAATAAATACATACCTCAGCAAGACGCACTAGAGGACAAAATCGTGGAATTTCAC
  CATAACCACATTGGACAAACACCAGCAGAATCAGATTTCCAGCTCCTAGAGATTGCCCGT
  CGGCTAGAGATGTATGGAATCCGGTTGCACCCGGCCAAGGACAGGGAAGGCACGAAGATC
  AATCTGGCCGTTGCCAACACGGGAATTCTAGTGTTTCAGGGTTTCACTAAGATCAATGCC
  TTCAACTGGGCCAAGGTGCGGAAGCTGAGCTTCAAGAGGAAGCGCTTTCTCATCAAGCTC
  CGGCCAGATGCCAATAGTGCGTACCAGGATACCTTGGAATTCCTGATGGCCAGTCGGGAT
  TTCTGCAAGTCCTTCTGGAAAATCTGTGTTGAACATCATGCCTTCTTTAGACTTTTTGAA
  GAGCCCAAACCAAAGCCCAAGCCCGTCCTCTTTAGCCGGGGGTCATCATTTCGGTTCAGT
  GGTCGGACTCAGAAGCAGGTTCTCGACTATGTTAAAGAAGGAGGACATAAGAAGGTGCAG
  TTTGAAAGGAAGCACAGCAAGATTCATTCTATCCGGAGCCTTGCTTCACAGCCTACAGAA
  CTGAATTCGGAAGTGCTGGAGCAGTCTCAGCAGAGCACCAGCCTTACATTTGGAGAAGGT
  GCCGAATCTCCAGGGGGCCAGAGCTGCCGGCGAGGAAAGGAACCGAAGGTTTCCGCCGGG
  GAGCCGGGGTCGCACCCGAGCCCTGCGCCGAGGAGAAGCCCCGCGGGTAACAAGCAGGCG
  GACGGAGCCGCCTCGGCGCCCACGGAGGAAGAGGAGGAGGTCGTTAAGGATAGGACCCAG
  CAGAGTAAACCTCAGCCCCCGCAGCCAAGCACAGGCTCCCTGACTGGCAGTCCTCACCTT
  TCCGAGCTGTCTGTGAACTCGCAGGGGGGAGTGGCCCCTGCCAACGTGACCTTGTCTCCC
  AACCTGAGCCCCGACACCAAGCAGGCCTCTCCCTTGATCAGCCCGCTGCTGAATGACCAG
  GCCTGCCCCCGGACGGACGATGAGGATGAGGGCCGGAGGAAGAGATTCCCAACTGATAAA
  GCGTACTTCATAGCTAAGGAAGTGTCTACCACCGAGCGAACATATCTGAAGGATCTCGAA
  GTTATCACTTCGTGGTTTCAGAGCACAGTGAGCAAAGAGGACGCCATGCCGGAAGCACTG
  AAAAGTCTCATATTCCCGAATTTTGAACCTTTGCACAAATTTCATACTAATTTTCTCAAG
  GAAATTGAGCAACGACTTGCCCTGTGGGAAGGCCGCTCAAATGCCCAAATCAGAGATTAC
  CAAAGAATCGGCGATGTCATGCTGAAGAACATTCAGGGCATGAAGCACCTGGCGGCTCAC
  CTGTGGAAGTACAGCGAGGCCTTGGAGGCCCTGGAGAATGGAATCAAGAGCTCCCGGCGG
  CTGGAGAACTTCTGCAGAGACTTTGAGCTGCAGAAGGTGTGTTACCTACCGCTCAACACC
  TTCCTCCTGCGGCCACTGCACCGGCTCATGCACTACAAGCAGGTCCTGGAGCGGCTGTGC
  AAACACCACCCGCCGAGCCACGCCGACTTCAGGGACTGCCGAGCCGCTTTGGCAGAGATC
  ACGGAGATGGTGGCACAGCTCCACGGTACGATGATCAAGATGGAGAATTTCCAGAAGCTG
  CACGAACTCAAGAAAGATTTGATTGGCATTGACAATCTTGTGGTTCCGGGAAGGGAGTTC
  ATCCGTCTGGGCAGCCTCAGCAAGCTCTCGGGGAAGGGGCTCCAGCAGCGCATGTTCTTC
  CTGTTCAACGACGTCCTGCTATACACGAGCCGGGGGCTGACGGCCTCCAATCAGTTTAAA
  GTCCACGGGCAGCTCCCGCTCTATGGCATGACGATTGAGGAGAGCGAAGACGAGTGGGGG
  GTGCCCCACTGCCTGACCCTCCGGGGCCAGCGGCAGTCCATCATCGTGGCCGCCAGTTCT
  CGGTCCGAGATGGAGAAGTGGGTTGAGGACATCCAGATGGCCATTGACCTGGCGGAGAAG
  AGCAGCAGCCCCGCCCCTGAGTTCCTGGCCAGCAGCCCCCCTGACAACAAGTCCCCTGAT
  GAAGCCACCGCGGCTGACCAGGAGTCAGAGGATGACCTGAGCGCCTCGCGCACATCGCTG
  GAGCGCCAGGCCCCGCACCGCGGCAACACAATGGTGCACGTGTGCTGGCACCGCAACACC
  AGCGTCTCCATGGTGGACTTCAGCATCGCAGTGGAGAATCAGTTGTCTGGAAACCTGCTG
  AGGAAATTCAAAAACAGCAACGGGTGGCAGAAGCTGTGGGTGGTGTTCACAAACTTCTGC
  CTGTTCTTCTACAAATCACACCAGGACAATCATCCCCTTGCCAGCCTGCCTCTGCTCGGC
  TACTCGCTCACCATCCCCTCTGAGTCCGAGAACATCCAGAAAGACTACGTGTTCAAGCTG
  CACTTCAAGTCCCACGTCTACTACTTCAGGGCGGAAAGCGAGTACACGTTCGAAAGGTGG
  ATGGAAGTGATCCGCAGTGCCACCAGCTCTGCCTCGCGACCCCACGTGTTGAGTCACAAA
  GAGTCTCTTGTGTATTGA
  
  Clone variation with respect to NM_005766.2
  1930 c=>t
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005766 unedited
  NNTTGTCANAATTTGTATACGACTCACTATAGGCGGCCGCGAATTCGCACGAGGCCTCAG
  CCGGCCACCGCCAGCCCTGCTCGCGCGCCCGCGCCGCCGCCGCCCGCGGGTATTAATAGC
  CGGCGCCGCCGCGCCCTCGGCCGCCGGGGGCTTGGGAGCCGCCGATCCCGGAGCCCGAGC
  CGGGAGAGGGAGCCGCCGCAGCCGCCGGCGCTGTGGAGATATTCTCTAAGCCGCTTTCAT
  CATGGGAGAAATAGAGCAGAGGCCGACCCCAGGATCACGACTGGGGGCCCCGGAAAATTC
  GGGGATCAGTACCTTGGAACGTGGACAGAAGCCGCCCCCAACACCTTCAGGAAAACTCGT
  GTCCATCAAAATCCAGATGCTGGATGACACCCAGGAGGCATTTGAAGTTCCACAAAGAGC
  TCCTGGGAAGGTGCTGCTGGATGCAGTTTGCAACCACCTCAACCTCGTGGAAGGTGACTA
  TTTTGGCCTCGAGTTTCCTGATCACAAAAAGATCACGGTGTGGCTGGATCTCCTAAAACC
  CATTGTGAAACAGATTAGAAGGCCAAAGCACGGTTTGTTGTTAAGTTTGTGGTGAAATTC
  TTTCCGCCTGACCACACACAACTCCAAGAAGAACTCACAAGGTACCTGTTCGCGCTGCAG
  GTGAAGCAGGACTTGGCTCAAGGGCAGTTGACGTGTAATGACACCAGCGCAGCTCTCTTG
  ATTTCACACATTGTGCAATCTGAGATTGGGGGATTNTGATGAAGCCTTGGACAGAGAGCA
  CTTAGCANAAAATAAATACATACCCTCAGCAGACGCACTAGAGGACAANAATCGTGGAAT
  NTCACCANTACCACATTGGACANACACCAGCAGAATCAGATTTCCAGCTCTTANAGATGC
  CCGTCGCTTNAANAGTATGAAATCCGA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005766 unedited
  TATAAGCGGCTTGCTAAGATCTAAACCCCACGNTGCCGGACTGGGGTTGGGTGTGCTGTT
  TTTTCAAAAACTGCGAGGCCGGGGTTAAAAAACGCGGCTGTGGAGACAGGAGAGCTGCTG
  GGAAGCCATGTTTTTGATTTTCAGACAGGTGTTAATTTTACCAGGCTTCATTAATACAGA
  AGAAAGGAAACGTCTTCCAGGAAAGCAGCCACTGCGGAAACGAGTGTGTCCGGCCATCAA
  TACACAAGAGACTCTTTGTGACTCAACACGTGGGGTCGCGAGGCAGAGCTGGTGGCACTG
  CGGATCACTTCCATCCACCTTTCGAACGTGTACTCGCTTTCCGCCCTGAAGTAGTAGACG
  TGGGACTTGAAGTGCAGCTTGAACACGTAGTCTTTCTGGATGTTCTCGGACTCAGAGGGG
  ATGGTGAGCGAGTAGCCGAGCAGAGGCAGGCTGGCAAGGGGATGATTGTCCTGGTGTGAT
  TTGTAGAAGAACAGGCAGAAGTTTGTGAACACCACCCACAGCTTCTGCCACCCGTTGCTG
  TTTTTGAATTTCCTCAGCAGGTTTCCAGACAACTGATTCTCCACTGCGATGCTGAAGTCC
  ACCATGGAGACGCTGGTGTTGCGGTGCCAGCACACGTGCACCATTGTGTTGCCGCGGTGC
  CGGGCCTGGCGCTCCAGCGATGTGCGCGAGGCGCTCAGGTCATCCTCTGACTCCTGGTCA
  GCCGCCGTGGCTTCATCAGGCGACTTGTTGTCAGGGGGGCTGCTGGCCAGGACTTAGGTG
  CGTGGCTGTTGCTCTTCTCCGCAGGTTAATGGGCATNTGGATGTCCTACACCACTTTCCA
  TCTGGGCCGAGACTGTCGCCCCATGATGACTGCGCTGCCCCGCAGGTCAGCGTGGGCACC
  CCCATCGTTTCGTTTCTTATAGCAGCCTATACGGAGTGTCCTGACTTAATGTGGAGCCGT
  AACCCGGTCGGTACAGACTCTTGAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005766
• Insert Size: 4160 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_005766.2, NP_005757.1
• RefSeq Size: 5028 bp
• RefSeq ORF: 3138 bp
• Locus ID: 10160
• UniProt ID: Q9Y4F1
• Cytogenetics: 13q32.2
• Domains: RhoGEF, B41, PH
• Gene Summary: This gene encodes a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2013]
  Transcript Variant: This variant (1) represents the longest transcript and encodes isoform 1.