CDC42 Binding Protein Kinase Beta (CDC42BPB) (NM_006035) Human Untagged Clone

CAT#: SC116371

CDC42BPB (untagged)-Human CDC42 binding protein kinase beta (DMPK-like) (CDC42BPB)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CDC42 Binding Protein Kinase Beta
• Synonyms: MRCKB
• Vector: pCMV6-XL6
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_006035 edited
  ATGTCGGCCAAGGTGCGGCTCAAGAAGCTGGAGCAGCTGCTCCTGGACGGGCCCTGGCGC
  AACGAGAGCGCCCTGAGCGTGGAAACGCTGCTCGACGTGCTCGTCTGCCTGTACACCGAG
  TGCAGCCACTCGGCCCTGCGCCGCGACAAGTACGTGGCCGAGTTCCTCGAGTGGGCTAAA
  CCATTTACACAGCTGGTGAAAGAAATGCAGCTTCATCGAGAAGACTTTGAAATAATTAAA
  GTAATTGGAAGAGGTGCTTTTGGTGAGGTTGCTGTTGTCAAAATGAAGAATACTGAACGA
  ATTTATGCAATGAAAATCCTCAACAAGTGGGAGATGCTGAAAAGAGCAGAGACCGCGTGC
  TTCCGAGAGGAGCGCGATGTGCTGGTGAACGGCGACTGCCAGTGGATCACCGCGCTGCAC
  TACGCCTTTCAGGACGAGAACCACCTGTACTTAGTCATGGATTACTATGTGGGTGGTGAT
  TTACTGACCCTGCTCAGCAAATTTGAAGACAAGCTTCCGGAAGATATGGCGAGGTTCTAC
  ATTGGTGAAATGGTGCTGGCCATTGACTCCATCCATCAGCTTCATTACGTGCACAGAGAC
  ATTAAACCTGACAATGTCCTTTTGGACGTGAATGGTCATATCCGCCTGGCTGACTTTGGA
  TCATGTTTGAAGATGAATGATGATGGCACTGTGCAGTCCTCCGTGGCCGTGGGCACACCT
  GACTACATCTCGCCGGAGATCCTGCAGGCGATGGAGGACGGCATGGGCAAATACGGGCCT
  GAGTGTGACTGGTGGTCTCTGGGTGTCTGCATGTATGAGATGCTCTATGGAGAAACGCCG
  TTTTATGCGGAGTCACTCGTGGAGACCTATGGGAAGATCATGAACCATGAAGAGCGATTC
  CAGTTCCCATCCCATGTCACGGATGTATCTGAAGAAGCGAAGGACCTCATCCAGAGACTG
  ATCTGCAGTAGAGAACGCCGGCTGGGGCAGAATGGAATAGAGGATTTCAAAAAGCATGCG
  TTTTTTGAAGGTCTAAATTGGGAAAATATACGAAACCTAGAAGCACCTTATATTCCTGAT
  GTGAGCAGTCCCTCTGACACATCCAACTTCGACGTGGATGACGACGTGCTGAGAAACACG
  GAAATATTACCTCCTGGTTCTCACACAGGCTTTTCTGGATTACATTTGCCATTCATTGGT
  TTTACATTCACAACGGAAAGCTGTTTTTCTGATCGAGGCTCTCTGAAGAGCATAATGCAG
  TCCAACACATTAACCAAAGATGAGGATGTGCAGCGGGACCTGGAGCACAGCCTGCAGATG
  GAAGCTTACGAGAGGAGGATTCGGAGGCTGGAACAGGAGAAGCTGGAGCTGAGCAGGAAG
  CTGCAAGAGTCCACCCAGACCGTGCAGTCCCTCCACGGCTCATCTCGGGCCCTCAGCAAT
  TCAAACCGAGATAAAGAAATCAAAAAGCTAAATGAAGAAATCGAACGCTTGAAGAATAAA
  ATAGCAGATTCAAACAGGCTCGAGCGACAGCTTGAGGACACAGTGGCGCTTCGCCAAGAG
  CGTGAGGACTCCACGCAGCGGCTGCGGGGGCTGGAGAAGCAGCACCGCGTGGTCCGGCAG
  GAGAAGGAGGAGCTGCACAAGCAACTGGTTGAAGCCTCAGAGCGGTTGAAATCCCAGGCC
  AAGGAACTCAAAGATGCCCATCAGCAGCGAAAGCTGGCCCTGCAGGAGTTCTCGGAGCTG
  AACGAGCGCATGGCAGAGCTCCGTGCCCAGAAGCAGAAGGTGTCCCGGCAGCTGCGAGAC
  AAGGAGGAGGAGATGGAGGTGGCCACGCAGAAGGTGGACGCCATGCGGCAGGAAATGCGG
  AGAGCTGAGAAGCTCAGGAAAGAGCTGGAAGCTCAGCTTGATGATGCTGTTGCTGAGGCC
  TCCAAGGAGCGCAAGCTTCGTGAGCACAGCGAGAACTTCTGCAAGCAAATGGAAAGCGAG
  CTGGAGGCCCTCAAGGTGAAGCAAGGAGGCCGGGGAGCGGGTGCCACCTTAGAGCACCAG
  CAAGAGATTTCCAAAATCAAATCCGAGCTGGAGAAGAAAGTCTTATTTTATGAAGAGGAA
  TTGGTCAGACGTGAGGCCTCCCATGTGCTAGAAGTGAAAAATGTGAAGAAGGAGGTGCAT
  GATTCAGAAAGCCACCAGCTGGCCCTGCAGAAAGAAATCTTGATGTTAAAAGATAAGTTA
  GAAAAGTCAAAGCGAGAACGGCATAACGAGATGGAGGAGGCAGTAGGTACAATAAAAGAT
  AAATACGAACGAGAAAGAGCGATGCTGTTTGATGAAAACAAGAAGCTAACTGCTGAAAAT
  GAAAAGCTCTGTTCCTTTGTGGATAAACTCACAGCTCAAAATAGACAGCTGGAGGATGAG
  CTGCAGGATCTGGCAGCCAAGAAGGAGTCAGTGGCCCACTGGGAAGCTCAGATTGCGGAA
  ATCATTCAGTGGGTCAGTGACGAGAAAGATGCCCGGGGTTACCTTCAAGCTCTTGCTTCC
  AAGATGACCGAAGAGCTCGAGGCTTTGAGGAGTTCTAGTCTGGGGTCAAGAACACTGGAC
  CCGCTGTGGAAGGTGCGCCGCAGCCAGAAGCTGGACATGTCCGCGCGGCTGGAGCTGCAG
  TCGGCCCTGGAGGCGGAGATCCGGGCCAAGCAGCTTGTCCAGGAGGAGCTCAGGAAGGTC
  AAGGACGCCAACCTCACCTTGGAAAGCAAACTAAAGGATTCCGAAGCCAAAAACAGAGAA
  TTATTAGAAGAAATGGAAATTTTGAAGAAAAAGATGGAAGAAAAATTCAGAGCAGATACT
  GGGCTCAAACTTCCAGATTTTCAGGATTCCATTTTTGAGTATTTCAACACTGCTCCTCTT
  GCACATGACCTGACATTTAGAACCAGCTCAGCTAGTGAGCAAGAAACACAAGCTCCGAAG
  CCAGAAGCGTCCCCGTCGATGTCTGTGGCTGCATCAGAGCAGCAGGAGCCAAAAGCTCAC
  CAGTTCAGCATCAAGTCCTTCTCCAGCCCTACTCAGTGCAGCCACTGCACCTCCCTGATG
  GTTGGGCTGATCCGGCAGGGCTACGCCTGCGAGGTGTGTTCCTTTGCTTGCCACGTGTCC
  TGCAAAGACGGTGCCCCCCAGGTGTGCCCAATACCTCCCGAGCAGTCCAAGAGGCCTCTG
  GGCGTGGACGTGCAGCGAGGCATCGGAACAGCCTACAAAGGCCATGTCAAGGTCCCAAAG
  CCCACGGGGGTGAAGAAGGGATGGCAGCGCGCATATGCAGTCGTCTGTGACTGCAAGCTC
  TTCCTGTATGATCTGCCTGAAGGAAAATCCACCCAGCCTGGTGTCATTGCGAGCCAAGTC
  TTGGATCTCAGAGATGACGAGTTTTCCGTGAGCTCAGTCCTGGCCTCAGATGTCATTCAT
  GCTACACGCCGAGATATTCCATGTATATTCAGGGTGACGGCCTCTCTCTTAGGTGCACCT
  TCTAAGACCAGCTCGCTGCTCATTCTGACAGAAAATGAGAATGAAAAGAGGAAGTGGGTT
  GGGATTCTAGAAGGACTCCAGTCCATCCTTCATAAAAACCGGCTGAGGAATCAGGTCGTG
  CATGTTCCCTTGGAAGCCTACGACAGCTCGCTGCCTCTCATCAAGGCCATCCTGACAGCT
  GCCATCGTGGATGCAGACAGGATTGCAGTCGGCCTAGAAGAAGGGCTCTATGTCATAGAG
  GTCACCCGAGATGTGATCGTCCGTGCCGCTGACTGTAAGAAGGTACACCAGATCGAGCTT
  GCTCCCAGGGAGAAGATTGTAATCCTCCTCTGTGGCCGGAACCACCATGTGCACCTCTAT
  CCGTGGTCGTCCCTTGATGGAGCGGAAGGCAGCTTTGACATCAAGCTTCCGGAAACCAAA
  GGCTGCCAGCTCATGGCCACGGCCACACTCAAGAGGAACTCTGGCACCTGCCTGTTTGTG
  GCCGTGAAACGGCTGATCCTTTGCTATGAGATCCAGAGAACGAAGCCATTCCACAGAAAG
  TTCAATGAGATTGTGGCTCCCGGCAGCGTGCAGTGCCTGGCGGTGCTCAGGGACAGGCTC
  TGTGTGGGCTACCCTTCTGGGTTCTGCCTGCTGAGCATCCAGGGGGACGGGCAGCCTCTA
  AACCTGGTAAATCCCAATGACCCCTCGCTTGCGTTCCTCTCACAACAGTCTTTTGATGCC
  CTTTGTGCTGTGGAGCTCGAAAGCGAGGAGTACCTGCTTTGCTTCAGCCACATGGGACTG
  TACGTGGACCCGCAAGGCCGGAGGGCACGCGCGCAGGAGCTCATGTGGCCTGCGGCTCCT
  GTCGCCTGTAGTTGCAGCCCCACCCACGTCACGGTGTACAGCGAGTATGGCGTGGACGTC
  TTTGATGTGCGCACCATGGAGTGGGTGCAGACCATCGGCCTGCGGAGGATAAGGCCCCTG
  AACTCTGAAGGCACCCTCAACCTCCTCAACTGCGAGCCTCCACGCTTGATCTACTTCAAG
  AGCAAGTTCTCGGGAGCGGTTCTCAACGTGCCGGACACCTCCGACAACAGCAAGAAGCAG
  ATGCTGCGCACCAGGAGCAAAAGGCGGTTCGTCTTCAAGGTCCCAGAGGAAGAGAGACTG
  CAGCAGAGGCGAGAGATGCTTAGAGACCCAGAATTGAGATCCAAAATGATATCCAACCCA
  ACCAACTTCAACCACGTGGCCCACATGGGCCCAGGCGACGGCATGCAGGTGCTCATGGAC
  CTGCCTCTGAGTGCTGTGCCCCCCTCCCAGGAGGAAAGGCCGGGCCCCGCTCCCACCAAC
  CTGGCTCGCCAGCCTCCATCCAGGAACAAGCCCTACATCTCGTGGCCCTCATCAGGTGGA
  TCGGAGCCTAGCGTGACTGTGCCTCTGAGAAGTATGTCTGATCCAGACCAGGACTTTGAC
  AAAGAGCCTGATTCGGACTCCACCAAACACTCAACTCCATCGAATAGCTCCAACCCCAGC
  GGCCCACCGAGCCCCAACTCCCCCCACAGGAGCCAGCTCCCCCTCGAAGGCCTGGAGCAG
  CCGGCCTGTGACACCTGAAGCCGCCAGCTCGCCACAGGGGCCAGGGAGCTGGAGATGGCC
  TCCAGCGTCAGTGCCAAGACTGAGCGGGCCCTCCAGTGTTGTCCAAGGAAATGTAGAATC
  ACTTTGTAGATATGGAGATGAAGAAGACAAATCTTTATTATAATATTGATCAGTTTTATG
  CCGCATTGTTCGTGGCAGTAGACCACATCTGTTCGTCTGCACAGCTGTGAGGCGATGCTG
  TTCCATCTGCACATGAAGGACCCCCATACAGCCTGTCTCCCACCCCTGACAACCCGAGAG
  GGCATATGGGGCCCTGCCAACACCACTTCCTCAGCAGAAACCCGTCATGACGCGGCTGCT
  TCGGAAGCAGACATCTGGGGACACAGCCTCAGTACCCAGTCTTTTCCCTAGTTCCTGAAA
  CTTTCCTAGGACCTTAAGAGAATAGTAGGAGGTCCTATAGCATTCCCAGTGTCACTAGAA
  TTTTGAAGACAGGAAAGTGGAGGTTAGTCTGTGGCCTTTTTTTCATTTAGCCATTGCACA
  GTCAGCTGCAGAAGTCCTGCTGACCACCTAGTCATGGACAAAGGCCCAGGACCAGTGACA
  CCCTGCGTCCCTGTGTGCGTTAAGTTCATTCTGGGTCGCAGCCATGAAGTGTCACCAGTA
  TCTACTACTGTGAAGTCAGCTGTGCTGTTTTCCATTCGCTTCCACGGCTTCTGCCTCCTG
  CCATAAAACCAGCGAGTGTCGTGGTGCAGGCAGGCCCTGTGGCCTGCTGGGCTGAGGGAA
  GTCAGAGCCCCAGGGCGCCACGAAGCAGCCACTGGGATACCCCACCCCGCCCCGCCCTGC
  CCGCCCCCCCCCCCCACCAGTCCTGCCCCCGCATGGAGCCCCCGTGATTAGTAGCCCGTA
  TGATCACGTAGACCCACCCAACACACTCCTGCACACTGGCCCCGGCCCACGGCACAGCAA
  TCCCCTGCGCGTGGATTTCACCTCACCCTTTGTACCAGATGTTGAGTGACCAGCTCTGTG
  GCCCTGTGTCGTCAGAGGCTTGTGATTAACTGTGGCGGCAGACACAGCTTGTCCACAGCT
  TGGGCCAGGCTTCCCCTGTCCTCCCACCGGTCGGCTGCTTGGCAAGGCTGTTCAGGACGT
  GCACTTCCCCAAGTCGGCACTGAGTGGCCCAGCACCGCCTAGCCCTGCCACCCCACTGCC
  CTCCTGGGCCTTCTGCTGGATGGGCACCTGGGGGGTTCTGGTTTTTACTTTTTTAATGTA
  AGTCTCAGTCTTTGTAATTAATTATTGAATTGTGAGAACATTTTTGAACAATTTACCTGT
  CAATAAAGCAGAAGACGGCAGTTTTAAAGTTGAAAAAAAAAAAAAAAAAACTCGACTCTA
  GATTGCGGCCGCGGTCATAGCTGTTTCCTGAACAGATCCCGGGTGGCATCCCTGTGACCC
  CTCCCCAGTGCCTCTCCTGGC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_006035 unedited
  TATCCCCCGCCCCGTTGNCGCAATGGGCGGTAGGCGTGTACGGTGGGAGGTCTATATAAG
  CAGAGCTCATTTAGGTGACACTATAGAATACAAGCTACTTGTTCTTTTTGCAGCGGCCGC
  GAATTCGGCACGAGGCCGAGCCCCCGAGGGCGCGCCCTGACGGACTGGCCGAGCCGGCGG
  TGAGAGGCCGGCGCGTCGGGAGCGGGCCGCGCGGCACCATGTCGGCCAAGGTGCGGCTCA
  AGAAGCTGGAGCAGCTGCTCCTGGACGGGCCCTGGCGCAACGAGAGCGCCCTGAGCGTGG
  AAACGCTGCTCGACGTGCTCGTCTGCCTGTACACCGAGTGCAGCCACTCGGCCCTGCGCC
  GCGACAAGTACGTGGCCGAGTTCCTCGAGTGGGCTAAACCATTTACACAGCTGGTGAAAG
  AAATGCAGCTTCATCGAGAAGACTTTGAAATAATTAAAGTAATTGGAAGAGGTGCTTTTG
  GTGAGGTTGCTGTTGTCAAAATGAAGAATACTGAACGAATTTATGCAATGAAAATCCTCA
  ACAAGTGGGAGATGCTGAAAAGAGCAGAGACCGCGTGCTTCCGAGAGGAGCGCGATGTGC
  TGGTGAACGGCGACTGCCAGTGGATCACCGCGCTGCACTACGCCTTTCAGGACGAGAACC
  ACCTGTACTTAGTCATGGATNTACTATGTGGGTGGTGATTTACTGACCCTGCTCAGCAAA
  TTTGAAAGACAGCTTCCGGAAGATATGGCGAGGTTCTACATTGGTGAAATGGTGCTGGCC
  ATTGACTNCATNCATCAGCTTCATTACGTGCACAGAGACATTAAACCCTGACATGTCCTT
  TNTGNNACGTGATGGTCATATCCGCCTGGCTGACTTNGGAATCATGTTTGAAGATGATGA
  TGATGGCACTGTGCAGTCCTCCTGGCCGTGGNGCACACTGACTACTCTTGNC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_006035 unedited
  GTCTGTGTACGCGGNCGCATTCTANGTCGAGTTATTTCTTTTTTTTTTTAAACTTTAAAA
  CTGCCGTCTTCTGCTTTATTGACAGGTAAATTGTTCAAAAATGTTCTCACAATTCAATAA
  TTAATTACAAAGACTGAGACTTACATTAAAAAAGTAAAAACCAGAACCCCCCAGGTGCCC
  ATCCAGCAGAAGGCCCAGGAGGGCAGTGGGGTGGCAGGGCTAGGCGGTGCTGGGCCACTC
  AGTGCCGACTTGGGGAAGTGCACGTCCTGAACAGCCTTGCCAAGCAGCCGACCGGTGGGA
  GGACAGGGGAAGCCTGGCCCAAGCTGTGGACAAGCTGTGTCTGCCGCCACAGTTAATCAC
  AAGCCTCTGACGACACAGGGCCACAGAGCTGGTCACTCAACATCTGGTACAAAGGGTGAG
  GTGAAATCCACGCGCAGGGGATTGCTGTGCCGTGGGCCGGGGCCAGTGTGCAGGAGTGTG
  TTGGGTGGGTCTACGTGATCATACGGGCTACTAATCACGGGGGCTCCATGCGGGGGCAGG
  ACTGGTGGGGGGGGGGGCGCGGNCAGGGCGGGGCGGGGTGGGGTATCCCAGTGGCTGCTT
  CGTGGCGCCCTGGGGCTCTGACTTCCCTCAGCCCAGCAGGCCACAGGGCCTGCCTGCACC
  ACGACACTCGCTGGTTTTATGGCAGGAGGCAGAAGCCGTGGAAGCGAATGGAAAACAGCA
  CAGCTGACTTACAGTAGTAGATACTGGTGACACTTCATGCCTGCGACCCAAATGAACTTA
  CGCACCCGGGACGCCGGGTGTACTGGTCCTGGGCCTTTGTCCTGACTAGGTGGCACCAGA
  CTTCTGCACTGACTGTGCATGGCTAATGAAAAAAAGGCCCCGACTACCCTCCACTTTCTG
  GCTTCCAAAATTTAGGCACCTGGA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_006035
• Insert Size: 6900 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006035.2, NP_006026.2
• RefSeq Size: 6782 bp
• RefSeq ORF: 5136 bp
• Locus ID: 9578
• UniProt ID: Q9Y5S2
• Cytogenetics: 14q32.32
• Domains: PBD, pkinase, S_TK_X, CNH, TyrKc, PH, DAG_PE-bind, S_TKc, M
• Protein Families: Druggable Genome, Protein Kinase
• Gene Summary: This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008]