TECPR2 (NM_014844) Human Untagged Clone

CAT#: SC114803

TECPR2 (untagged)-Human tectonin beta-propeller repeat containing 2 (TECPR2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TECPR2
• Synonyms: KIAA0329; SPG49
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014844, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCATCGATATCAGAGCCTGTTACATTCAGAGAGTTCTGCCCGTTGTACTATCTCCTCAATGCCATTC
  CGACAAAGATCCAGAAGGGTTTCCGCTCTATCGTGGTCTATCTCACGGCCCTCGACACCAACGGGGACTA
  CATCGCGGTGGGCAGCAGCATCGGCATGCTCTATCTGTACTGCCGGCACCTCAACCAGATGAGGAAGTAC
  AACTTTGAGGGGAAGACGGAATCTATCACTGTGGTGAAGCTGCTGAGCTGCTTTGATGACCTGGTGGCAG
  CAGGCACAGCCTCTGGCAGGGTTGCAGTTTTTCAACTTGTATCTTCATTGCCAGGGAGAAATAAACAGCT
  TCGGAGATTTGATGTCACTGGTATTCACAAAAATAGCATTACAGCTCTGGCTTGGAGCCCCAATGGAATG
  AAATTGTTCTCTGGAGATGACAAAGGCAAAATTGTTTATTCTTCTCTGGATCTAGACCAGGGGCTCTGTA
  ACTCCCAGCTGGTGTTGGAGGAGCCATCTTCCATTGTGCAGCTGGATTATAGCCAGAAAGTGCTGCTGGT
  CTCTACTCTGCAAAGAAGTCTGCTCTTTTACACTGAAGAAAAGTCTGTAAGGCAAATTGGAACACAACCA
  AGGAAAAGTACTGGGAAATTTGGTGCTTGTTTTATACCAGGACTCTGTAAGCAAAGTGATCTAACCTTGT
  ATGCGTCACGGCCCGGGCTCCGGCTATGGAAGGCTGATGTCCACGGGACTGTTCAAGCCACGTTTATCTT
  AAAAGATGCTTTTGCCGGGGGAGTCAAGCCTTTTGAACTGCACCCGCGTCTGGAATCCCCCAACAGTGGA
  AGTTGCAGCTTACCTGAGAGGCACCTGGGGCTTGTTTCATGTTTCTTTCAAGAAGGCTGGGTGCTGAGTT
  GGAATGAATATAGTATCTATCTCCTAGACACAGTCAACCAGGCCACAGTTGCTGGTTTGGAAGGATCCGG
  TGATATTGTGTCTGTTTCGTGCACAGAAAATGAAATATTTTTCTTGAAAGGAGATAGGAACATTATAAGA
  ATTTCAAGCAGGCCTGAAGGATTAACATCAACAGTGAGAGATGGTCTGGAGATGTCTGGATGCTCAGAGC
  GTGTCCACGTGCAGCAAGCGGAGAAGCTGCCAGGGGCCACAGTTTCTGAGACGAGGCTCAGAGGCTCTTC
  CATGGCCAGCTCCGTGGCCAGCGAGCCAAGGAGCAGGAGCAGCTCGCTCAACTCCACCGACAGCGGCTCC
  GGGCTCCTGCCCCCTGGGCTCCAGGCCACCCCTGAGCTGGGCAAGGGCAGCCAGCCCCTGTCACAGAGAT
  TCAACGCCATCAGCTCAGAGGACTTTGACCAGGAGCTTGTCGTGAAGCCTATCAAAGTGAAAAGGAAGAA
  GAAGAAGAAGAAGACAGAAGGTGGAAGCAGGAGCACCTGTCACAGCTCCCTGGAATCGACACCCTGCTCC
  GAATTTCCTGGGGACAGTCCCCAGTCCTTGAACACAGACTTGCTGTCGATGACCTCAAGTGTCCTGGGCA
  GTAGCGTGGATCAGTTAAGTGCAGAGTCTCCAGACCAGGAAAGCAGCTTCAATGGTGAAGTGAACGGTGT
  CCCACAGGAAAATACTGACCCCGAAACGTTTAATGTCCTGGAGGTGTCAGGATCAATGCCTGATTCTCTG
  GCTGAGGAAGATGACATTAGAACTGAAATGCCACACTGTCACCATGCACATGGGCGGGAGCTGCTCAATG
  GAGCGAGGGAAGATGTGGGAGGCAGTGATGTCACGGGACTCGGAGATGAGCCGTGTCCTGCAGATGATGG
  ACCAAATAGCACACAGTTACCCTTCCAAGAACAGGACAGCTCTCCTGGGGCGCATGATGGGGAAGACATC
  CAACCCATTGGCCCCCAAAGCACTTTTTGTGAAGTCCCCCTCCTGAACTCACTCACTGTGCCTTCCAGCC
  TCAGCTGGGCCCCAAGTGCTGAACAGTGGCTGCCTGGGACCAGAGCTGATGAAGGCAGCCCCGTGGAGCC
  CAGCCAAGAGCAGGACATCCTAACCAGCATGGAGGCCTCTGGCCACCTCAGCACAAATCTCTGGCATGCT
  GTCACTGATGATGACACAGGTCAGAAAGAAATACCCATTTCTGAACGTGTCTTGGGGAGTGTGGGAGGAC
  AGCTGACTCCGGTCTCTGCCTTGGCAGCCAGCACTCACAAGCCCTGGCTTGAGCAGCCTCCACGGGATCA
  GACATTGACGTCCAGCGATGAGGAGGACATCTATGCCCACGGGCTTCCTTCTTCATCCTCAGAGACGAGT
  GTGACAGAGCTCGGACCTAGTTGCTCCCAGCAGGACCTGAGCCGGCTGGGTGCAGAGGACGCCGGGCTGC
  TCAAGCCAGATCAGTTTGCAGAAAGCTGGATGGGCTACTCGGGTCCCGGCTATGGCATCCTCAGCTTGGT
  GGTCTCCGAGAAGTATATCTGGTGCCTGGACTACAAAGGCGGCCTGTTCTGCAGCGCGTTGCCGGGCGCC
  GGGCTGCGCTGGCAGAAGTTTGAAGATGCTGTCCAGCAGGTGGCAGTCTCGCCCTCAGGAGCCCTTCTCT
  GGAAGATTGAACAGAAATCTAACCGGGCTTTTGCTTGTGGGAAAGTCACCATCAAGGGGAAGCGGCACTG
  GTACGAAGCCCTGCCCCAGGCAGTGTTTGTGGCCCTGAGCGATGACACGGCCTGGATCATCAGGACCAGT
  GGGGACCTATACTTGCAGACAGGTCTGAGCGTGGATCGCCCTTGTGCCAGAGCCGTAAAGGTGGACTGTC
  CCTACCCGCTGTCCCAGATCACAGCCCGGAACAATGTGGTGTGGGCGCTGACAGAGCAGAGGGCCCTCCT
  GTACCGGGAGGGCGTGAGCAGCTTCTGTCCGGAAGGCGAGCAGTGGAAGTGTGACATTGTCAGCGAAAGG
  CAAGCTTTAGAACCCGTCTGCATAACGCTCGGGGATCAGCAGACTCTCTGGGCCCTGGACATCCATGGGA
  ACCTGTGGTTCAGAACTGGCATTATTTCCAAGAAGCCCCAAGGAGATGACGACCATTGGTGGCAAGTGAG
  CATCACGGACTATGTGGTGTTTGACCAGTGCAGCTTATTTCAGACGATAATCCATGCCACTCACTCGGTG
  GCCACAGCAGCCCAAGCCCCCGTAGAAAAGGTGGCAGATAAGCTGCGCATGGCGTTTTGGTCCCAGCAGC
  TTCAGTGCCAGCCAAGCCTTCTCGGGGTCAATAACAGCGGTGTCTGGATCTCCTCGGGCAAGAATGAATT
  CCACGTCGCTAAGGGAAGTCTCATAGGCACCTACTGGAATCATGTGGTTCCCCGTGGGACAGCTTCTGCT
  ACAAAATGGGCCTTTGTGTTGGCTTCTGCAGCTCCCACGAAGGAAGGAAGCTTCCTGTGGCTGTGCCAGA
  GCAGCAAGGACCTGTGCAGCGTCAGCGCCCAGAGCGCACAGTCGCGGCCCTCCACGGTGCAGCTGCCTCC
  CGAAGCCGAGATGCGCGCCTATGCCGCCTGCCAGGATGCGCTGTGGGCGCTGGACAGCCTCGGCCAGGTG
  TTCATCAGGACGCTCTCCAAGAGCTGCCCCACGGGCATGCACTGGACCAGGCTGGACCTCTCCCAGCTAG
  GAGCTGTAAAATTGACAAGCTTGGCATGTGGAAATCAGCACATCTGGGCCTGTGATTCCAGGGGTGGAGT
  TTACTTCCGTGTAGGGACTCAGCCTCTCAATCCCAGTCTCATGCTTCCAGCCTGGATAATGATTGAGCCA
  CCTGTCCAGCCCGCCGGGGTCAGCTTGGTCAGCGTCCATTCCAGCCCCAACGACCAGATGCTGTGGGTGC
  TTGACAGCAGGTGGAACGTGCACGTGCGGACCGGGATCACCGAGGAGATGCCTGTGGGGACCGCCTGGGA
  GCATGTGCCAGGGTTGCAGGCCTGCCAGCTGGCGCTGAGCACCAGGACCGTGTGGGCCCGCTGTCCAAAC
  GGAGACCTCGCCCGGCGGTACGGCGTCACAGACAAGAACCCCGCCGGGGACTACTGGAAGAAAATTCCCG
  GCAGCGTGTCGTGTTTCACAGTGACTGCGTCAGATGAGCTGTGGGCTGTGGGCCCGCCCGGCTACCTCCT
  CCAACGGCTGACAAAGACGTTCAGCCACTCGCACGGCACCCAGAAGAGCAGCCAGGCCGCCATGCCCCAC
  CCTGAGGACCTGGAGGACGAGTGGGAGGTCATCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014844 unedited
  GGCGGCCCGCGAATTCGCACGAGGGAGCCCCCAGGTTTCCCTAGATGACAAATAAACATT
  CCTTTTCCTGCGTGAAGATAGTCTGTGGAAACCTTGGCCATGGCATCGATATCAGAGCCT
  GTTACATTCAGAGAGTTCTGCCCGTTGTACTATCTCCTCAATGCCATTCCGACAAAGATC
  CAGAAGGGTTTCCGCTCTATCGTGGTCTATCTCACGGCCCTCGACACCAACGGGGACTAC
  ATCGCGGTGGGCAGCAGCATCGGCATGCTCTATCTGTACTGCCGGCACCTCAACCAGATG
  AGGAAGTACAACTTTGAGGGGAAGACGGAATCTATCACTGTGGTGAAGCTGCTGAGCTGC
  TTTGATGACCTGGTGGCAGCAGGCACAGCCTCTGGCAGGGTTGCAGTTTTTCAACTTGTA
  TCTTCATTGCCAGGGAGAAATAAACAGCTTCGGAGATTTGATGTCACTGGTATTCACAAA
  AATAGCATTACAGCTCTGGCTTGGAGCCCCAATGGAATGAAATTGTTCTCTGGAGATGAC
  AAAGGCAAAATTGTTTATTCTTCTCTGGATCTAGACCAGGGGCTCTGTAACTCCCAGCTG
  GTGTTGGAGGAGCCATCTTCCATTGTGCAGCTGGATTATAGCCAGAAAGTGCTGCTGGTC
  TCTACTCTGCAAAGAAGTCTGCTCTTTTACACTGAAGAAAAGTCTGTAAGGCANATTTGG
  AACACAACCAAGGAAAAGTACTGGGGAAATTTGGTGCTTGTTTTATACCAAGACTCCTGT
  AGCAAGTGATCTAACCTGTATGCGTCACGGCCCGGGCTTCGGCTATGGAGGCTGATGTCA
  CGGGACTGTTCAAGCCAGTTTATCTTAAAGATGCCTTTGCGGGGAAGTCAACCTTTTGAC
  TGCCCCGGTCGGGATCCCCAAAATGAAGT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014844
• Insert Size: 5000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014844.2, NP_055659.1
• RefSeq Size: 8703 bp
• RefSeq ORF: 8703 bp
• Locus ID: 9895
• UniProt ID: O15040
• Cytogenetics: 14q32.31
• Domains: WD40, TECPR
• Gene Summary: The protein encoded by this gene is a member of the tectonin beta-propeller repeat-containing (TECPR) family, and contains both TECPR and tryptophan-aspartic acid repeat (WD repeat) domains. This gene has been implicated in autophagy, as reduced expression levels of this gene have been associated with impaired autophagy. Recessive mutations in this gene have been associated with a hereditary form of spastic paraparesis (HSP). HSP is characterized by progressive spasticity and paralysis of the legs. There is also some evidence linking mutations in this gene with birdshot chorioretinopathy (BSCR), which results in inflammation of the choroid and retina. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Aug 2015]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the longer isoform (1). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.