CKLF (NM_016326) Human Untagged Clone

CAT#: SC114315

CKLF (untagged)-Human chemokine-like factor (CKLF), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CKLF
• Synonyms: C32; CKLF1; CKLF2; CKLF3; CKLF4; HSPC224; UCK-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC114315 sequence for NM_016326 edited (data generated by NextGen Sequencing)
  ATGGATAACGTGCAGCCGAAAATAAAACATCGCCCCTTCTGCTTCAGTGTGAAAGGCCAC
  GTGAAGATGCTGCGGCTGGTGTTTGCACTTGTGACAGCAGTATGCTGTCTTGCCGACGGG
  GCCCTTATTTACCGGAAGCTTCTGTTCAATCCCAGCGGTCCTTACCAGAAAAAGCCTGTG
  CATGAAAAAAAAGAAGTTTTGTAA
  
  Clone variation with respect to NM_016326.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016326 unedited
  TGCACATTTGTATACGACTCATATAGGCGGCCCGCGAATTCGCACGAGGGTCTGCAGACG
  CGATGGATAACGTGCAGCCCGAAAATAAAACATCGCCCCTTCTGCTTCAGTGTGAAAGGC
  CACGTGAAGATGCTGCGGCTGGATATTATCAACTCACTGGTAACAACAGTATTCATGCTC
  ATCGTATCTGTGTTGGCACTGATACCAGAAACCACAACATTGACAGTTGGTGGAGGGGTG
  TTTGCACTTGTGACAGCAGTATGCTGTCTTGCCGACGGGGCCCTTATTTACCGGAAGCTT
  CTGTTCAATCCCAGCGGTCCTTACCAGAAAAAGCCTGTGCATGAAAAAAAAGAAGTTTTG
  TAATTTTATATTACTTTTTAGTTTGATACTAAGTATTAAACATATTTCTGTATTCTTCCA
  CAAAAAAAAAAAAAAAAAAAAACTCGACTCTAGATTGCGGCCGCGGTCATAGCTGTTTCC
  TGAACAGATCCCGGGTGGCATCCCTGTGACCCCTCCCCAGTGCCTCTCCTGGCCCTGGAA
  GTTGCCACTCCAGTGCCCACCAGCCTTGTCCTAATAAAATTAAGTTGCATCATTTTGTCT
  GACTAGGTGTCCTTCTATAATATTATGGGGTGGAGGGGGGTGGGTATGGACCAAGGGGCA
  AGTTGGGAACACACCCTGTAGGGCCCGCGGGGGCTATTGGGAACCAACCTGGAGTGCATT
  GGAACAATCTTGGCCTAATGAATTTTCCCGCTTCTGGGTTCAAGCGATTTTCCTGCCCTC
  AACCTCCCGAGTTGGTGGGATTCCCAGCATTCGATGCCCAGTCTTAACCAAATCTCCTTC
  TCTTGCACAAACCGTGTTTTACCCTTTTTGGCCTTCTCGGCTCCCACTCTCTACCTATTC
  GGCACCTACCCTCCTCGTTCTCCCACATGGTG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_016326 unedited
  NNTTTTTGCTCTGGACCGGCGGCCGCATNCTAGGATCGAGTTTTTTTTTTTTTTTTTTTT
  TGGGGAAGAATACAGAATATGTTTAATACTTAGTATCAAACTAAAAAGTAATATAAAATT
  ACAAAACTTCTTTTTTTTCATGCACAGGCTTTTTCTGGTAAGGACCGCTGGGATTGAACA
  GAAGCTTCCGGTAAATAAGGGCCCCGTCGGCAAGACAGCATACTGCTGTCACAAGTGCAA
  ACACCCCTCCACCAACTGTCAATGTTGTGGTTTCTGGTATCAGTGCCAACACAGATACGA
  TGAGCATGAATACTGTTGTTACCAGTGAGTTGATAATATCCAGCCGCAGCATCTTCACGT
  GGCCTTTCACACTGAAGCAGAAGGGGCGATGTTTTATTTTCGGCTGCACGTTATCCATCG
  CGTCTGCAGACCCCTCGTGCCGAATTCGCGGCCGCCCTATAGTGAGTCGTATTACAAAAT
  TCTGACGGTTCACTAAACGAGCTCTGCTTATATAGACCTCCCACCGTACACGCCTACCGG
  CCATTTGCGTNAACGGGGCGGAGTTATTACGACATTTCGGAACGCCCCGTTGATTTTCGG
  CGCCAAAAAACTCCCATTGATCGTCATGGCGGTGACACCTCGCACATCCCCCCCGGTAAA
  ACCCGTCCCCACCCCCCTCGCTGGTATGGCCGAAACCGCCCCCCCTGGCCATACACAGAA
  ATAAAAACTAAAGCCCCCCCCCTCCCCACCACCGCCTCAGGCTTTCCCCCTCGTTTCTAT
  CCCTCCCGTCATCCCCTCGCCTCCCCACCCCTACCGCCCTCTCTTGGGTCCCGCCCCGCC
  GTAATCCGACCACGCAGCCCCCGTCGACCTGCCACATACCACCTCGCCCCACCCCTCTAC
  TCCCTN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016326
• Insert Size: 430 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016326.2, NP_057410.1
• RefSeq Size: 434 bp
• RefSeq ORF: 204 bp
• Locus ID: 51192
• UniProt ID: Q9UBR5
• Cytogenetics: 16q21
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Gene Summary: The product of this gene is a cytokine. Cytokines are small proteins that have an essential role in the immune and inflammatory responses. This gene is one of several chemokine-like factor genes located in a cluster on chromosome 16. The protein encoded by this gene is a potent chemoattractant for neutrophils, monocytes and lymphocytes. It also can stimulate the proliferation of skeletal muscle cells. This protein may play important roles in inflammation and in the regeneration of skeletal muscle. Alternatively spliced transcript variants encoding different isoforms have been identified. Naturally occurring read-through transcription occurs between this locus and the neighboring locus CMTM1 (CKLF-like MARVEL transmembrane domain containing 1).[provided by RefSeq, Feb 2011]
  Transcript Variant: This variant (3) lacks two an in-frame exons in the coding region, compared to variant 1. It encodes a shorter isoform (c), also known as an isoform CKL3.