Protocadherin beta 12 (PCDHB12) (NM_018932) Human Untagged Clone

CAT#: SC113330

PCDHB12 (untagged)-Human protocadherin beta 12 (PCDHB12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Protocadherin beta 12
• Synonyms: PCDH-BETA12
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC113330 sequence for NM_018932 edited (data generated by NextGen Sequencing)
  ATGGAAAACGGAGGGGCAGGCACTCTGCAGATAAGGCAAGTCCTGCTTTTCTTTGTTTTG
  CTGGGAATGTCTCAGGCGGGCTCTGAAACTGGGAACTTTTTGGTGATGGAGGAATTGCAG
  AGCGGGAGCTTTGTAGGAAATTTGGCAAAGACCCTGGGACTCGAGGTGAGTGAGCTGTCT
  TCGCGGGGGGCTCGGGTGGTTTCTAATGATAACAAAGAGTGTTTGCAGCTGGACACAAAC
  ACTGGGGATTTGCTCCTGAGAGAAATGCTAGACAGGGAGGAGCTCTGTGGCTCCAATGAG
  CCTTGTGTGCTGTATTTCCAAGTGTTAATGAAAAACCCCACGCAGTTTTTACAAATTGAG
  CTCCAGGTCAGGGATATAAATGATCACTCTCCCGTCTTCTTGGAAAAAGAAATGCTCTTA
  GAAATCCCAGAGAACAGTCCTGTTGGTGCTGTGTTCTTGCTTGAAAGTGCAAAGGATTTA
  GATGTAGGAATCAATGCTGTAAAAAGCTACACAATAAATCCGAACTCTCATTTCCACGTT
  AAAATAAGAGTCAATCCAGACAATAGGAAATACCCTGAGTTAGTTCTGGACAAGGCGCTG
  GATTATGAAGAGCGCCCGGAGCTCAGTTTCATCCTCACTGCTCTGGATGGCGGGTCCCCT
  CCCAGGTCTGGAACTGCCTTGGTCAGGGTGGTGGTTGTAGATATTAATGACAACTCCCCT
  GAGTTTGAGCAGGCTTTTTATGAGGTGAAGATTCTGGAGAATAGCATCCTTGGCTCCCTG
  GTTGTGACCGTCTCAGCCTGGGATTTAGACTCTGGAACAAACAGTGAACTATCCTATACC
  TTTTCCCATGCCTCAGAAGATATTCGCAAGACATTTGAAATTAATCAAAAGTCTGGTGAC
  ATTACTTTAACAGCACCTTTGGATTTTGAAGCAATTGAGTCATACTCAATAATCATTCAA
  GCCACAGATGGGGGAGGACTTTTTGGAAAATCTACAGTCAGAATTCAGGTGATGGATGTA
  AACGACAACGCTCCTGAAATCACTGTGTCATCAATTACCAGTCCAATCCCAGAAAACACT
  CCAGAGACTGTGGTTATGGTTTTCAGGATACGAGACAGAGACTCTGGGGACAACGGAAAG
  ATGGTTTGTTCTATCCCGGAGGACATCCCATTCGTGCTAAAATCTTCGGTAAATAATTAC
  TACACTTTGGAAACAGAGAGACCGCTGGACAGAGAGAGCAGAGCCGAGTACAACATCACC
  ATCACCGTCACCGACTTGGGGACCCCCAGGCTAAAAACCGAGCACAACATAACCGTGCTG
  GTCTCCGACGTCAATGACAACGCCCCCGCCTTCACCCAAACTTCCTACGCCCTGTTCGTC
  CGCGAGAACAACAGCCCCGCCCTGCACATCGGCAGCATCAGCGCCACAGACAGAGACTCG
  GGCACCAACGCCCAGGTCAACTACTCGCTGCTGCCGTCCCAGGACCCGCACCTGCCCCTC
  GCCTCCCTGGTCTCCATCAACGCGGACAACGGCCACCTGTTTGCCCTCAGGTCGCTGGAC
  TACGAGGCCCTGCAGGGGTTCCAGTTCCGCGTGGGCGCCACAGACCACGGCTCCCCGGCT
  TTGAGCAGCGAGGCGCTGGTGCGCGTGCTGGTGCTGGACGCCAACGACAACTCGCCCTTC
  GTGCTGTACCCGCTGCAGAACGGCTCCGCGCCCTGCACCGAGCTGGTGCCCTGGGCGGCC
  GAGCCGGGCTACCTGGTGACCAAGGTGGTGGCGGTGGACGGTGACTCGGGCCAGAACGCC
  TGGCTGTCGTACCAGCTGCTCAAGGCCACGGAGCCCGGGCTATTCGGCGTGTGGGCGCAC
  AATGGCGAGGTGCGCACCGCCAGGCTGCTGAGCGAGCGCGACGCGGCCAAGCACAGGCTG
  GTGGTGCTGGTCAAGGACAATGGCGAGCCTCCGCGCTCGGCCACCGCCACGCTGCACGTG
  CTCCTGGTGGACGGCTTCTCCCAGCCCTACCTGCCTCTCCCGGAGGCGGCCCCGGCCCAG
  GCCCAGGCCGACTCGCTCACTGTCTACCTGGTGGTGGCGTTGGCCTCAGTGTCGTCGCTC
  TTCCTCTTCTCGGTGCTCCTGTTCGTGGCGGTGCGGCTGTGCAGGAGGAGCAGGGCGGCC
  CCGGTCGGTCGCTGCTCGGTGCCTGAGGGCCCCTTTCCAGGACATCTGGTGGACGTGAGT
  GGCACCGGGACCCTGTCCCAGAGCTACCACTATGAGGTGTGTGTGACTGGAGGCTCCAGG
  TCAAATAAGTTCAAATTTCTGAAACCAATTATCCCCAACTTCCTACCCCAGAGCACAGGT
  AGTGAAGTCGAAGAAAATCCCCCATTTCAGAATAATTTGGGTTTCTGA
  
  Clone variation with respect to NM_018932.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018932 unedited
  TTTTGTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGGAAAANAAGCTATNG
  GAAAACGGAGGGGCAGGCACTCTGCAGATAAGGCAAGTCCTGCTTTTCTTTGTTTTGCTG
  GGAATGTCTCAGGCGGGCTCTGAAACTGGGAACTTTTTGGTGATGGAGGAATTGCAGAGC
  GGGAGCTTTGTAGGAAATTTGGCAAAGACCCTGGGACTCGAGGTGAGTGAGCTGTCTTCG
  CGGGGGGCTCGGGTGGTTTCTAATGATAACAAAGAGTGTTTGCAGCTGGACACAAACACT
  GGGGATTTGCTCCTGAGAGAAATGCTAGACAGGGAGGAGCTCTGTGGCTCCAATGAGCCT
  TGTGTGCTGTATTTCCAAGTGTTAATGAAAAACCCCACGCAGTTTTTACAAATTGAGCTC
  CAGGTCAGGGATATAAATGATCACTCTCCCGTCTTCTTGGAAAAAGAAATGCTCTTAGAA
  ATCCCAGAGAACAGTCCTGTTGGTGCTGTGTTCTTGCTTGAAAGTGCAAAGGATTTAGAT
  GTAGGAATCAATGCTGTAAAAAGCTACACAATAAATCCGAACTCTCATTTCCACGTTAAA
  ATAAGAGTCAATCCAGACAATAGGAAATACCCTGAGTTAGTTCTGGACAAGGCGCTGGAT
  TATGAAGAGCGCCCGGAGCTCAGTTTCATCCTCACTGCTCTGGATGGCGGNGTCCCCTCC
  CAGGTCTGGAACTGCCTTGGTCAGGNTGGTGGTTGTAGATATTAAATGACACTCCCNCTG
  AGTTGAGCAGGGCTTTTTATGAGTGAAGATTCTGGAGAATAGCATCCTTGGCTCCCTGGT
  TGTGACCGTCTCAGCCTGGGAATAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018932
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018932.3, NP_061755.1
• RefSeq Size: 3422 bp
• RefSeq ORF: 2388 bp
• Locus ID: 56124
• UniProt ID: Q9Y5F1
• Cytogenetics: 5q31.3
• Protein Families: Transmembrane
• Gene Summary: This gene is a member of the protocadherin beta gene cluster, one of three related gene clusters tandemly linked on chromosome five. The gene clusters demonstrate an unusual genomic organization similar to that of B-cell and T-cell receptor gene clusters. The beta cluster contains 16 genes and 3 pseudogenes, each encoding 6 extracellular cadherin domains and a cytoplasmic tail that deviates from others in the cadherin superfamily. The extracellular domains interact in a homophilic manner to specify differential cell-cell connections. Unlike the alpha and gamma clusters, the transcripts from these genes are made up of only one large exon, not sharing common 3' exons as expected. These neural cadherin-like cell adhesion proteins are integral plasma membrane proteins. Their specific functions are unknown but they most likely play a critical role in the establishment and function of specific cell-cell neural connections. [provided by RefSeq, Jul 2008]