Noxa (PMAIP1) (NM_021127) Human Untagged Clone

CAT#: SC112973

PMAIP1 (untagged)-Human phorbol-12-myristate-13-acetate-induced protein 1 (PMAIP1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Noxa
• Synonyms: APR; NOXA
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_021127 edited
  GAATTCGGCACGAGGGTAGTTGGCATCTCCGCGCGTCCGGACACCCGATCCCAGCATCCC
  TGCCTGCAGGACTGTTCGTGTTCAGCTCGCGTCCTGCAGCTGTCCGAGGTGCTCCAGTTG
  GAGGCTGAGGTTCCCGGGCTCTGTAGCTGAGTGGGCGGCGGCACCGGCGGAGATGCCTGG
  GAAGAAGGCGCGCAAGAACGCTCAACCGAGCCCCGCGCGGGCTCCAGCAGAGCTGGAAGT
  CGAGTGTGCTACTCAACTCAGGAGATTTGGAGACAAACTGAACTTCCGGCAGAAACTTCT
  GAATCTGATATCCAAACTCTTCTGCTCAGGAACCTGACTGCATCAAAAACTTGCATGAGG
  GGACTCCTTCAAAAGAGTTTTCTCAGGAGGTGCACGTTTCATCAATTTGAAGAAAGACTG
  CATTGTAATTGAGAGGAATGTGAAGGTGCATTCATGGGTGCCCTTGGAAACGGAAGATGG
  AATACATCAAAGTGAATTTCTGTTCAAGTTTTCCCAGATTATCATTCTTTGGGATGAGAG
  AACATTATAAAACCACTTTGTTTATTTTAAAGCAAGAATGGAAGACCCTTGAAAATAAAG
  AAGTAATTATTGACACATTTCTTTTTTACTTAGAGAATCGTTCTAGTGTTTTTGCCGAAG
  ATTACCGCTGGCCTACTGTGAAGGGAGATGACCTGTGATTAGACTGGGCGGCTGGGGAGA
  AACAGTTCAGTGCATTGTTGTTGTTGCTGTTTTTGGTGTTTTGCTTTTCAGTGCCAACTC
  AGCACATTGTATATGATTCGGTTTATACATATTACCTTGTTATAATGAAAAAACTCATTC
  TGAGAACACTGAAATGTTATACTCAGTGTTGATTTCTTCGGTCACTACACAACGTAAAAT
  CATTTGTTTCTTTTGACTCAAATTGTATTGCTTCTGTTCAGATGATCTTTCATTCAATGT
  GTTCCTGTTGGGCGTTACTAGAAACTATGGAAAACTGGAAAATAACTTTGAAAAAATTGG
  ATAAAGTATAGGAGGGTTACTTGGGGCCAGTAAATCAGTAGACTGAACATTCAATATAAT
  AAAAGAACATGGGGATTTTGTATAACCAGGGATAATAAAAAGAAAAAGAAGTTAATTTTT
  AATTGATGTTTTTGAAACTTAGTAGAACAAATATTCAGAAGTAACTTGATAAGATATGAA
  TGTTTCTAAAGAAGTTTCTAAAGGTTCGGAAAATGCTCCTTGTCACATTAGTGTGCATCC
  TACAAAAAGTGATCTCTTAATGTAAATTAAGAATATTTTCATAATTGGAATATACTTTTC
  TTAAAAAAAAGGAACAGTTAGTTCTCATCTAGAATGAAAGTTCCATATATGCATTGGTGA
  ATATATATGTATACACATACTTACATACTTATATGGGTATCTGTATAGATAATTTGTATT
  AGAGTATTATATAGCTTCTTAGTAGGGTCTCAAGTAAGTTTCATTTTTTTTATCTGGGCT
  ATATACAGTCCTCAAATAAATAATGTCTTGATTTTATTTCAGCAGGAATAATTTTATTTA
  TTTTGCCTATTTATAATTAAAGTATTTTTCTTTAGTTTGAAAATGTGTATTAAAGTTACA
  TTTTTGAGTTACAAGAGTCTTATAACTACTTGAATTTTTAGTTAAAATGTCTTAATGTAG
  GTTGTAGTCACTTTAGATGGAAAATTACCTCACATCTGTTTTCTTCAGTATTACTTAAGA
  TTGTTTATTTAGTGGTAGAGAGTTTTTTTTTCAGCCTAGAGGCAGCTATTTTACCATCTG
  GTATTTATGGTCTAATTTGTATTTAAACATATGCACACATATAAAAGTTGATACTGTGGC
  XXXXXXXXXXXXXXXXXXXXXXXXXXCTCGAC
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_021127 unedited
  TGTTAGATTTGTATACGACTCCTATAGGCGGCCCGCGATTCGGCACGAGGGTAGTTGGCA
  TCTCCGCGCGTCCGGACACCCGATCCCAGCATCCCTGCCTGCAGGACTGTTCGTGTTCAG
  CTCGCGTCCTGCAGCTGTCCGAGGTGCTCCAGTTGGAGGCTGAGGTTCCCGGGCTCTGTA
  GCTGAGTGGGCGGCGGCACCGGCGGAGATGCCTGGGAAGAAGGCGCGCAAGAACGCTCAA
  CCGAGCCCCGCGCGGGCTCCAGCAGAGCTGGAAGTCGAGTGTGCTACTCAACTCAGGAGA
  TTTGGAGACAAACTGAACTTCCGGCAGAAACTTCTGAATCTGATATCCAAACTCTTCTGC
  TCAGGAACCTGACTGCATCAAAAACTTGCATGAGGGGACTCCTTCAAAAGAGTTTTCTCA
  GGAGGTGCACGTTTCATCAATTTGAAGAAAGACTGCATTGTAATTGAGAGGAATGTGAAG
  GTGCATTCATGGGTGCCCTTGGAAACGGAAGATGGAATACATCAAAGTGAATTTCTGTTC
  AAGTTTTCCCAGATTATCATTCTTTGGGATGAGAGAACATTATAAAACCACTTTGTTTAT
  TTTAAAGCAAGAATGGAAGACCCTTGAAAATAAAGAAGTAATTATTGACACATTTCTTTT
  TTACTTAGAGAATCGTTCTAGTGTTTTTGCCGAAGATTACCGCTGGCCTACTGTGAAGGG
  AGATGACCTGTGATTAGACTGGGCGGCTGGNGAGAACAGTTCAGTGCATTGNTGNTGTTG
  CTGTTTTTGGTGTTTGCTTTTCAGTGCCAACTCAGCACATTGTATATGAAATCGTTTATA
  CATATTACCTTTGTTATATGAAAAACTCATTCTGAAACACTGNNAATGTATACTCAGTGT
  TGATTNCTTNCGTCCTAA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_021127 unedited
  GAGTCGAGTCCTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTAA
  ACAGGGAAAACTTTTAATATTTTACTGCCACAGTATCAACTTTTATTTGGGGGCATTTGT
  TTAAATACAAATTAAACCTTAAATCCCCGAGGGGAAAATAGCTGCCTTTAGGGTGAAAAA
  AAAACTTTTTACCACTAAATAAACAATTTTAACTAATACTGAAAAAAACAAATGGGGGGG
  AATTCTCCATTTAAAGGGACTACAACCTACTTTAAAACTTTTTAACTAAAAATTCAAGTA
  TTTATAAAACTTTTGGAACTAAAAAATGTAATTTTATCCCCCATTTTAAAACTAAAAAAA
  AACCCTTTAATTATAAATCGGCCAACTAAATAAATTTTTTCCTGCTGAAATAAACACAAA
  ACATTTTTTTTCTGAGGACGGTTTATCCCCCATAAAAAAAAATGAAATTTATTTGAAACC
  CTTCTAAAAAGCTTTATAATACTTCTACCCAAATTTTTTTACCCACCCCCCTTTACTTCG
  CAGCCCGGGTTACTTTTTCTTCCCAATGTCATAGGAACCCCCCTCCCAAGAAAACTATGT
  GTCTCTCCTTAACAAAACTCCCCCCCTCCGAAACTTCTACTTCCTTAAAAAAACTCTTCG
  GGGGAGTCCATAAGGGCCCGCCCATTTTCAACCTTTAAACTTTTTAAAAACCCCTCCCTT
  TTCTCTTTTATTTATTTTGCTCCCTCCTCCCCCCCCTATTCACATTACTTTTTTCTTTTT
  TTATACGCGGCTCCCCATCTTCTCCCCCCTTTTTATGCCTATTCTTATTCCCGCCCCACC
  CCTTTTTCCCCCTTCTTTTTTCCTTTCCTCCCCCCTTCTGCTTTTATTTTCCCCTTCCCC
  CCCTTCCCCATTTTCCCCTCCCTCCTCCTTTTCTTCCCCCCCCCCCCCTCCTCTTTTCCC
  CCCCC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_021127
• Insert Size: 2090 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021127.1, NP_066950.1
• RefSeq Size: 1885 bp
• RefSeq ORF: 165 bp
• Locus ID: 5366
• UniProt ID: Q13794
• Cytogenetics: 18q21.32
• Protein Families: Druggable Genome, Stem cell - Pluripotency
• Protein Pathways: p53 signaling pathway
• Gene Summary: This gene belongs to a pro-apoptotic subfamily within the BCL-2 protein family, referred to as the BCL-2 homology domain 3 (BH3)-only subfamily, which determine whether a cell commits to apoptosis. In response to death-inducing stimuli, BH3-only members inhibit the anti-apoptotic BCL-2 family members, which under steady-state conditions keep the multi-BH domain proteins BAX and BAK, in an inactive state. [provided by RefSeq, Aug 2020]