MRPL17 (NM_022061) Human Untagged Clone

CAT#: SC112571

MRPL17 (untagged)-Human mitochondrial ribosomal protein L17 (MRPL17), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MRPL17
• Synonyms: L17mt; LIP2; MRP-L17; MRP-L26; RPL17L; RPML26
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC112571 sequence for NM_022061 edited (data generated by NextGen Sequencing)
  ATGCGGCTGTCGGTCGCTGCAGCGATCTCCCATGGCCGCGTATTTCGCCGTATGGGCCTC
  GGTCCCGAGTCCCGCATCCATCTGTTGCGGAACTTGCTCACAGGGCTGGTGCGGCACGAA
  CGCATCGAGGCACCATGGGCGCGTGTGGACGAAATGAGGGGCTACGCGGAGAAGCTCATC
  GACTATGGGAAGCTGGGAGACACTAACGAACGAGCCATGCGCATGGCTGACTTCTGGCTC
  ACAGAGAAGGATTTGATCCCAAAGCTGTTTCAAGTACTGGCCCCTCGGTACAAAGATCAA
  ACTGGGGGCTACACAAGAATGCTGCAGATCCCAAATCGGAGTTTGGATCGGGCCAAGATG
  GCAGTGATCGAGTATAAAGGGAATTGCCTCCCACCCCTGCCTCTGCCTCGCAGAGACAGC
  CACCTTACACTCCTAAACCAGCTGCTGCAGGGTTTGCGGCAGGACCTCAGGCAAAGCCAG
  GAAGCAAGCAACCACAGCTCCCACACAGCTCAAACACCAGGGATTTAA
  
  Clone variation with respect to NM_022061.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022061 unedited
  GGTTGCGCCAATATGTACTACGTTCTTCACTATAGGGCGGCCGCGAATTCGCACGAGCAG
  AATTGGAAACATGCGGCTGTCGGTCGCTGCAGCGATCTCCCATGGCCGCGTATTTCGCCG
  TATGGGCCTCGGTCCCGAGTCCCGCATCCATCTGTTGCGGAACTTGCTCACAGGGCTGGT
  GCGGCACGAACGCATCGAGGCACCATGGGCGCGTGTGGACGAAATGAGGGGCTACGCGGA
  GAAGCTCATCGACTATGGGAAGCTGGGAGACACTAACGAACGAGCCATGCGCATGGCTGA
  CTTCTGGCTCACAGAGAAGGATTTGATCCCAAAGCTGTTTCAAGTACTGGCCCCTCGGTA
  CAAAGATCAAACTGGGGGCTACACAAGAATGCTGCAGATCCCAAATCGGAGTTTGGATCG
  GGCCAAGATGGCAGTGATCGAGTATAAAGGGAATTGCCTCCCACCCCTGCCTCTGCCTCG
  CAGAGACAGCCACCTTACACTCCTAAACCAGCTGCTGCAGGGTTTGCGGCAGGACCTCAG
  GCAAAGCCAGGAAGCAAGCAACCACAGCTCCCACACAGCTCAAACACCAGGGATTTAACT
  GGATCTGAAGAGTCTGCAGCCCTTAATCAGTACCCATGATCACAGGGCCTTTGGAGCACT
  TTTACTCTCTGAGAAGAACTGGAGCTAGAGATGTAAAATGGACAGTCTTGATGGGGTTGA
  GAACCTTCCTGGGAGCCAGATGACCCTCTCTTTGACCATAGATAAAGTCTTTATATGAAA
  TATATAAATTATTTATTTTTTCCTTTCTGCGGGATTTCTGCAGAATGAGATTATTCAAAT
  GCCTCATCTACCTGAAATAGAAATTCATGCCTAAGCTTTTGGCCCTAAAAAAAAAANCGC
  NCTCTCCTCCATTCCCACAACACCT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_022061 unedited
  AGCACTGCGGGACANAAACAGCCTCGACTTCTCAGTAGACCGAGCATTTGGATAATTCTC
  ATTCTCCANAAATCCCACAGGNAAGGGAAAAAATAAATAAATTTATATATATTCATATAA
  AGACTTTTATCTATTGTGCAAAGAGAGGGTCATCTGGCTCCCCAGAAGGTTCTCAACCCC
  ATCAAGACTGTCCATTTTACATCTCTAGCTCCAGTTCTTCTCAGAGAGTAAAAGTGCTCC
  AAACGCCTGTGATCATGGGTACTGATTAAGGGCTGCAGACTCTTCAGATCCAGTTAAATC
  CCTGGTGTTTGAGCTGTGTGGGAGCTGTGGTTGCTTGCTTCCTGGCTTTGCCTGAGGTCC
  TGCCGCAAACCCTGCAGCAGCTGGTTTAGGAGTGTAAGGTGGCTGTCTCTGCGAGGCAGA
  GGCAGGGGTGGGAGGCAATTCCCTTTATACTCGATCACTGCCATCTTGGCCCGATCCAAA
  CTCCGATTTGGGATCTGCAGCATTCTTGTGTAGCCCCCAGTTTGATCTTTGTACCGAGGG
  GCCAGTTCTTGAAACAGCTTTGGGATCAAATCCTTCTCTGTGAGCCAGAAGTCAGCCATG
  CGCATGGCTCGTTCGTTAGTGTCTCCCAGCTTTCCATATTCGATGAGCTTCTTCCCGTAG
  CCCCTTATTTCGTCACACGCGCCCCTGGTGCCTCCATGCGTTCGTGCCCCACCAGCCCTG
  TGAGCAAGTTCCCTTCCGACGGTGGCCGGACTCCGGCCCCAGCCCTTACGGCGAATACCC
  CGCCCTGTGGAGATCCTTGCACGCCCGCCAGTCCCTGTTCCCACCTTCGCTCTCGCCCCA
  TTTCCGGCCCCCCTTTTTGGCCCCTTCCCAAATTCTGCCGGTCCACAAACCACTTCGCTT
  ATAAAACAA
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022061
• Insert Size: 880 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022061.2, NP_071344.1
• RefSeq Size: 1044 bp
• RefSeq ORF: 528 bp
• Locus ID: 63875
• UniProt ID: Q9NRX2
• Cytogenetics: 11p15.4
• Domains: Ribosomal_L17
• Gene Summary: Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. [provided by RefSeq, Jul 2008]