PARP12 (NM_022750) Human Untagged Clone

CAT#: SC112439

PARP12 (untagged)-Human poly (ADP-ribose) polymerase family, member 12 (PARP12)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PARP12
• Synonyms: ARTD12; MST109; MSTP109; ZC3H1; ZC3HDC1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_022750, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCCCAGGCCGGCGTCGTCGGTGAGGTCACCCAGGTGCTGTGCGCGGCCGGGGGCGCCCTGGAGTTGC
  CCGAGCTGCGGCGCCGCTTGCGGATGGGCTTGAGCGCCGACGCGCTGGAGCGGCTGCTGCGGCAGCGTGG
  GCGCTTCGTGGTGGCGGTGCGGGCGGGCGGCGCAGCCGCGGCCCCGGAGCGCGTGGTGCTGGCCGCCTCG
  CCGCTGCGCCTGTGTCGCGCGCACCAGGGCTCCAAGCCGGGCTGCGTGGGGCTCTGCGCGCAGCTCCACC
  TCTGCAGGTTCATGGTCTACGGCGCCTGCAAGTTCCTGAGAGCCGGGAAGAACTGTAGGAATAGTCACAG
  CTTGACAACCGAACACAACCTGAGTGTGCTGAGAACTCATGGCGTTGACCACCTGAGCTATAATGAGCTA
  TGCCAACTCTTGTTTCAGAACGACCCCTGGCTTTTGCCAGAAATTTGCCAACATTACAACAAAGGAGATG
  GACCCCACGGCTCTTGTGCCTTTCAAAAGCAGTGCATCAAGCTCCATATCTGCCAGTATTTTTTACAGGG
  GGAATGCAAGTTTGGCACTAGCTGTAAGAGATCCCATGATTTCTCTAATTCTGAGAATCTGGAAAAATTG
  GAGAAGTTGGGTATGAGCTCAGACCTGGTGAGCAGGCTGCCTACCATTTATAGAAATGCACATGACATCA
  AGAATAAGAGCTCTGCCCCCAGCAGAGTGCCTCCTCTTTTTGTCCCACAGGGGACTTCTGAAAGAAAAGA
  CAGTTCAGGTTCTGTGTCCCCAAACACTCTTAGCCAGGAGGAGGGTGATCAGATCTGTTTGTACCATATC
  CGGAAAAGTTGTAGCTTTCAAGATAAGTGCCATAGAGTTCATTTCCATTTGCCGTATCGATGGCAATTCT
  TGGATAGAGGCAAATGGGAGGATTTGGACAACATGGAACTTATTGAAGAGGCATATTGCAATCCCAAAAT
  AGAAAGGATCCTGTGCTCTGAGTCAGCCAGTACCTTTCACTCTCATTGTCTGAACTTTAACGCCATGACT
  TACGGTGCTACCCAGGCTCGCCGCCTCTCCACGGCCTCCTCTGTCACCAAACCTCCACACTTCATCCTCA
  CCACTGACTGGATTTGGTACTGGAGTGATGAGTTTGGTTCTTGGCAGGAATATGGAAGACAGGGCACGGT
  GCACCCTGTGACCACTGTCAGCAGTAGCGACGTGGAGAAGGCCTACCTGGCCTACTGTACACCGGGGTCT
  GACGGCCAGGCAGCCACCTTGAAGTTCCAGGCCGGAAAGCACAACTACGAGTTAGATTTCAAAGCCTTCG
  TTCAGAAAAACCTGGTCTATGGCACAACTAAAAAGGTTTGCCGCAGACCCAAATACGTGTCTCCCCAGGA
  TGTGACGACCATGCAAACCTGCAATACCAAGTTTCCAGGCCCGAAGAGCATCCCAGACTATTGGGACTCC
  TCTGCCCTGCCAGACCCAGGCTTTCAGAAGATCACCCTTAGTTCTTCCTCGGAAGAGTATCAGAAGGTCT
  GGAACCTCTTTAACCGCACGCTGCCTTTCTACTTTGTTCAGAAGATTGAGCGAGTACAGAACCTGGCCCT
  CTGGGAAGTCTACCAGTGGCAAAAAGGACAGATGCAGAAGCAGAATGGAGGGAAGGCCGTGGACGAGCGG
  CAGCTGTTCCACGGCACCAGCGCCATTTTTGTGGACGCCATCTGCCAGCAGAACTTTGACTGGCGGGTCT
  GTGGTGTTCATGGCACTTCCTACGGCAAGGGGAGCTACTTTGCCCGAGATGCTGCATATTCCCACCACTA
  CAGCAAATCCGACACGCAGACCCACACGATGTTCCTGGCCCGGGTGCTGGTGGGCGAGTTCGTCAGGGGC
  AATGCGTCCTTTGTCCGTCCGCCGGCCAAGGAGGGCTGGAGCAACGCCTTCTATGATAGCTGCGTGAACA
  GTGTGTCCGACCCCTCCATCTTTGTGATCTTTGAGAAACACCAGGTCTACCCAGAGTATGTCATCCAGTA
  CACCACCTCCTCCAAGCCCTCGGTCACACCCTCCATCCTGCTGGCCTTGGGCTCCCTGTTCAGCAGCCGA
  CAGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_022750 unedited
  GTTGGGTATTTGTGATACGACTCACTATAGGGGCGGCCGCGATTCGGCACGAGGCACCCT
  GCGAGAGCCGCCCGCCAGCCAGCGTCCGCCGCCGTCCGCGTCGCGCCACCCGCGGTCCGA
  CGGGAGCAGGCCCAGCGGCCATGGCCCAGGCCGGCGTCGTCGGTGAGGTCACCCAGGTGC
  TGTGCGCGGCCGGGGGCGCCCTGGAGTTGCCCGAGCTGCGGCGCCGCTTGCGGATGGGCT
  TGAGCGCCGACGCGCTGGAGCGGCTGCTGCGGCAGCGTGGGCGCTTCGTGGTGGCGGTGC
  GGGCGGGCGGCGCAGCCGCGGCCCCGGAGCGCGTGGTGCTGGCCGCCTCGCCGCTGCGCC
  TGTGTCGCGCGCACCAGGGCTCCAAGCCGGGCTGCGTGGGGCTCTGCGCGCAGCTCCACC
  TCTGCAGGTTCATGGTCTACGGCGCCTGCAAGTTCCTGAGAGCCGGGAAGAACTGTAGGA
  ATAGTCACAGCTTGACAACCGAACACAACCTGAGTGTGCTGAGAACTCATGGCGTTGACC
  ACCTGAGCTATAATGAGCTATGCCAACTCTTGTTTCAGAACGACCCCTGGCTTTTGCCAG
  AAATTTGCCAACATTACCACANAGGAGATGGACCCCACGGCTCTTGTGCCTTTCAAAAGC
  AGTGCATCAAGCTCCATATCTGCCAGTATTTTTTACAGGGGGAATGCAAGTTTGGCACTA
  GCTGTAAGAGATCCCATGATTTCTCTAATTCTGAGGATCTNGAAAAATTGGAGAAGTTGG
  GTATGAGCTCAGACCTGGTGAGCAAGGCTGCTACCATTATAGAAATGCACATGACATCAA
  GAATAAGAGCTCTGCCCCCAGCAGAGTGCCTCCTCTTTTTGTCCACAGGGGACTTNCTGA
  AGAAAGACAGTTCAGGTTCTGTGTCCCCAAACACTCTAGCCAGNAGAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_022750
• Insert Size: 3000 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022750.2, NP_073587.1
• RefSeq Size: 3805 bp
• RefSeq ORF: 2106 bp
• Locus ID: 64761
• UniProt ID: Q9H0J9
• Cytogenetics: 7q34
• Domains: zf-CCCH
• Gene Summary: Mono-ADP-ribosyltransferase that mediates mono-ADP-ribosylation of target proteins.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) represents the longer transcript and encodes the protein.