PDLIM7 (NM_005451) Human Untagged Clone

CAT#: SC111943

PDLIM7 (untagged)-Human PDZ and LIM domain 7 (enigma) (PDLIM7), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PDLIM7
• Synonyms: LMP1; LMP3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_005451, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATTCCTTCAAAGTAGTGCTGGAGGGGCCAGCACCTTGGGGCTTCCGGCTGCAAGGGGGCAAGGACT
  TCAATGTGCCCCTCTCCATTTCCCGGCTCACTCCTGGGGGCAAAGCGGCGCAGGCCGGAGTGGCCGTGGG
  TGACTGGGTGCTGAGCATCGATGGCGAGAATGCGGGTAGCCTCACACACATCGAAGCTCAGAACAAGATC
  CGGGCCTGCGGGGAGCGCCTCAGCCTGGGCCTCAGCAGGGCCCAGCCGGTTCAGAGCAAACCGCAGAAGG
  CCTCCGCCCCCGCCGCGGACCCTCCGCGGTACACCTTTGCACCCAGCGTCTCCCTCAACAAGACGGCCCG
  GCCCTTTGGGGCGCCCCCGCCCGCTGACAGCGCCCCGCAGCAGAATGGACAGCCGCTCCGACCGCTGGTC
  CCAGATGCCAGCAAGCAGCGGCTGATGGAGAACACAGAGGACTGGCGGCCGCGGCCGGGGACAGGCCAGT
  CGCGTTCCTTCCGCATCCTTGCCCACCTCACAGGCACCGAGTTCATGCAAGACCCGGATGAGGAGCACCT
  GAAGAAATCAAGCCAGGTGCCCAGGACAGAAGCCCCAGCCCCAGCCTCATCTACACCCCAGGAGCCCTGG
  CCTGGCCCTACCGCCCCCAGCCCTACCAGCCGCCCGCCCTGGGCTGTGGACCCTGCGTTTGCCGAGCGCT
  ATGCCCCGGACAAAACGAGCACAGTGCTGACCCGGCACAGCCAGCCGGCCACGCCCACGCCGCTGCAGAG
  CCGCACCTCCATTGTGCAGGCAGCTGCCGGAGGGGTGCCAGGAGGGGGCAGCAACAACGGCAAGACTCCC
  GTGTGTCACCAGTGCCACAAGGTCATCCGGGGCCGCTACCTGGTGGCGCTGGGCCACGCGTACCACCCGG
  AGGAGTTTGTGTGTAGCCAGTGTGGGAAGGTCCTGGAAGAGGGTGGCTTCTTTGAGGAGAAGGGCGCCAT
  CTTCTGCCCACCATGCTATGACGTGCGCTATGCACCCAGCTGTGCCAAGTGCAAGAAGAAGATTACAGGC
  GAGATCATGCACGCCCTGAAGATGACCTGGCACGTGCACTGCTTTACCTGTGCTGCCTGCAAGACGCCCA
  TCCGGAACAGGGCCTTCTACATGGAGGAGGGCGTGCCCTATTGCGAGCGAGACTATGAGAAGATGTTTGG
  CACGAAATGCCATGGCTGTGACTTCAAGATCGACGCTGGGGACCGCTTCCTGGAGGCCCTGGGCTTCAGC
  TGGCATGACACCTGCTTCGTCTGTGCGATATGTCAGATCAACCTGGAAGGAAAGACCTTCTACTCCAAGA
  AGGACAGGCCTCTCTGCAAGAGCCATGCCTTCTCTCATGTGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005451 unedited
  GTACGTCAAATTTTGTAAACGACTCACTATAGGCGGCCGCGAATTCGCACCAGGCTCCCT
  GGAGCCCGACGCAGAGCAGCGCCCTGGCCGGGCCAAGCAGGAGCCGGCATCATGGATTCC
  TTCAAAGTAGTGCTGGAGGGGCCAGCACCTTGGGGCTTCCGGCTGCAAGGGGGCAAGGAC
  TTCAATGTGCCCCTCTCCATTTCCCGGCTCACTCCTGGGGGCAAAGCGGCGCAGGCCGGA
  GTGGCCGTGGGTGACTGGGTGCTGAGCATCGATGGCGAGAATGCGGGTAGCCTCACACAC
  ATCGAAGCTCAGAACAAGATCCGGGCCTGCGGGGAGCGCCTCAGCCTGGGCCTCAGCAGG
  GCCCAGCCGGTTCAGAGCAAACCGCAGAAGGTGCAGACCCCTGACAAACAGCCGCTCCGA
  CCGCTGGTCCCAGATGCCAGCAAGCAGCGGCTGATGGAGAACACAGAGGACTGGCGGCCG
  CGGCCGGGGACAGGCCAGTCGCGTTCCTTCCGCATCCTTGCCCACCTCACAGGCACCGAG
  TTCATGCAAGACCCGGATGAGGAGCACCTGAAGAAATCAAGCCAGGTGCCCAGGACAGAA
  GCCCCAGCCCCAGCCTCATCTACACCCCAGGAGCCCTGGCCTGGCCCTACCGCCCCCAGC
  CCTACCAGCCGCCCGCCCTGGGCTGTGGACCCTGCGTTTGCCGAGCGCTATGCCCCGGAC
  AAAACGAGCACAGTGCTGACCCGGCACAGCCAGCCAGCCACGCCCACGCCGCTGCAGAGC
  CGCACCTCCATTGTGCAGGCAGCTGCCGGNAGGGTGCCAGGAGGGGGCAGCAACAACGGC
  AAGACTCCCGTGTGTCACCAGTGCCACAAGGTCATCCGGGGCCGCTACCTGGTGGCGCT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005451
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005451.3, NP_005442.2
• RefSeq Size: 1709 bp
• RefSeq ORF: 1374 bp
• Locus ID: 9260
• UniProt ID: Q9NR12
• Cytogenetics: 5q35.3
• Domains: PDZ, LIM
• Protein Families: Druggable Genome
• Gene Summary: The protein encoded by this gene is representative of a family of proteins composed of conserved PDZ and LIM domains. LIM domains are proposed to function in protein-protein recognition in a variety of contexts including gene transcription and development and in cytoskeletal interaction. The LIM domains of this protein bind to protein kinases, whereas the PDZ domain binds to actin filaments. The gene product is involved in the assembly of an actin filament-associated complex essential for transmission of ret/ptc2 mitogenic signaling. The biological function is likely to be that of an adapter, with the PDZ domain localizing the LIM-binding proteins to actin filaments of both skeletal muscle and nonmuscle tissues. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) encodes the longest isoform (1).