LSG1 (NM_018385) Human Untagged Clone

CAT#: SC108402

LSG1 (untagged)-Human large subunit GTPase 1 homolog (S. cerevisiae) (LSG1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LSG1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_018385, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGACGGGCCCTTATGCGCCATCAGACTCAGCGGA
  GCCGAAGCCATCGTCACACTGACTCCTGGTTGCACACAAGTGAACTCAATGATGGCTATGATTGGGGTCG
  TCTTAATCTTCAGTCAGTGACTGAACAGAGCTCCCTTGATGACTTCCTTGCTACTGCAGAACTTGCAGGA
  ACAGAGTTTGTAGCTGAGAAACTTAATATTAAGTTTGTGCCTGCTGAGGCTAGAACTGGACTACTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAAGCTCCATGAAGAAAACAAACAGTTCTTGTGTATACCGAGGAGACC
  AAACTGGAACCAAAATACTACCCCAGAAGAACTCAAACAAGCAGAGAAAGATAACTTTCTAGAATGGAGA
  CGTCAGCTTGTCCGGCTAGAAGAGGAACAGAAGCTGATATTGACTCCATTTGAACGAAATTTGGACTTTT
  GGCGCCAGCTCTGGAGAGTCATTGAGAGAAGTGATATTGTGGTCCAGATAGTAGATGCTCGAAACCCACT
  CCTGTTTAGATGTGAGGATTTGGAATGTTATGTGAAAGAAATGGATGCCAATAAGGAGAACGTCATTCTG
  ATCAACAAGGCAGACTTGCTGACTGCTGAGCAGCGGAGTGCCTGGGCCATGTACTTCGAAAAAGAAGATG
  TGAAGGTTATTTTCTGGTCAGCTTTGGCCGGAGCCATTCCCCTGAATGGTGACTCTGAGGAAGAGGCAAA
  CAGAGATGATAGACAAAGCAACACAACCAAGTTTGGACATTCCAGTTTCGACCAGGCTGAAATTTCCCAC
  AGTGAATCCGAACATCTCCCAGCTAGGGATTCTCCTTCACTTAGTGAAAATCCCACAACGGATGAAGATG
  ACAGTGAGTATGAGGACTGTCCAGAGGAGGAGGAAGACGACTGGCAGACGTGCTCAGAAGAAGACGGTCC
  CAAGGAAGAGGACTGCAGCCAGGACTGGAAGGAAAGCTCTACTGCAGATTCTGAGGCTCGGAGCAGGAAA
  ACCCCACAGAAGAGGCAGATACACAATTTTAGCCATCTGGTATCCAAGCAGGAGTTACTGGAGCTCTTTA
  AGGAGCTACACACTGGGAGAAAGGTGAAAGATGGGCAACTTACGGTCGGACTGGTGGGCTACCCTAATGT
  TGGTAAGAGTTCAACAATCAACACCATCATGGGCAACAAGAAAGTATCTGTGTCTGCCACACCTGGTCAC
  ACAAAGCACTTTCAGACTCTCTATGTGGAGCCTGGCCTCTGCCTGTGTGACTGTCCTGGCTTGGTGATGC
  CATCTTTTGTGTCTACCAAGGCAGAAATGACTTGCAGCGGAATCCTCCCAATTGATCAGATGAGAGATCA
  TGTTCCTCCTGTATCACTAGTTTGCCAGAATATTCCAAGACATGTTTTAGAAGCTACCTATGGCATTAAC
  ATCATAACGCCTAGAGAGGATGAAGATCCCCACCGACCTCCAACATCGGAAGAACTGTTGACAGCTTATG
  GATACATGCGAGGATTCATGACAGCGCATGGACAGCCAGACCAGCCTCGATCTGCGCGCTACATCCTGAA
  GGACTATGTCAGTGGTAAGCTGCTGTACTGCCATCCTCCTCCTGGAAGAGATCCTGTAACTTTTCAGCAT
  CAACACCAGCGACTCCTAGAGAACAAAATGAACAGTGATGAAATAAAAATGCAGCTAGGCAGAAATAAAA
  AAGCAAAGCAGATTGAAAATATCGTTGACAAAACTTTTTTCCATCAAGAGAATGTGAGGGCTTTGACCAA
  AGGAGTCCAGGCTGTGATGGGTTACAAGCCCGGGAGTGGTGTAGTGACTGCATCCACTGCGAGCTCTGAG
  AACGGGGCGGGGAAGCCCTGGAAAAAACATGGCAACAGAAATAAAAAAGAAAAAAGTCGTAGACTCTACA
  AGCACCTGGATATGTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_018385 unedited
  TCAGATTTTGTAATACGACTTCACTATAGGGCGGCCGCGAATTCGCACGAGGGGAAGCTT
  CGTCCAGCGGTCGTGTTGCCATGGGCCGGAGGAGAGCCCCGGCCGGTGGGTCGCTGGGAC
  GGGCCCTTATGCGCCATCAGACTCAGCGGAGCCGAAGCCATCGTCACACTGACTCCTGGT
  TGCACACAAGTGAACTCAATGATGGCTATGATTGGGGTCGTCTTAATCTTCAGTCAGTGA
  CTGAACAGAGCTCCCTTGATGACTTCCTTGCTACTGCAGAACTTGCAGGAACAGAGTTTG
  TAGCTGAAAAACTTAATATTAAGTTTGTGCCTGCTGAGGCTAGAACTGGACTACTGTCTT
  TCGAGGAGAGCCAGAGAATTAAGAAGCTCCATGAAGAAAACAAACAGTTCTTGTGTATAC
  CGAGGAGACCAAACTGGAACCAAAATACTACCCCAGAAGAACTCAAACAAGCAGAGAAAG
  ATAACTTTCTAGAATGGAGACGTCAGCTTGTCCGGCTAGAAGAGGAACAGAAGCTGATAT
  TGACTCCATTTGAACGAAATTTGGACTTTTGGCGCCAGCTCTGGAGAGTCATTGAGAGAA
  GTGATATTGTGGTCCAGATAGTAGATGCTCGAAACCCACTCCTGTTTAGATGTGAGGATT
  TGGAATGTTATGTGAAAGAAATGGATGCCAATAAGGAGAACGTCATTCTGATCAACAAGG
  CAGACTTGCTGACTGCTGAGCAGCGGAGTGCCTNGGCCATGTACTTCGAAAAGAAGATGT
  GAAGGTTATTTTCTGGTCAGCTTTGGCCGGAGCCATTCCCCTGAATGGTGACTCTNGAGA
  AAGAGGCAACAGAGATGATAGACANAGNNCACACACTGAGTTNGGACATTNCAGTTNCGA
  CCAGNCTGAAATTNCCACAGTGAATNCCGACATCTCCAGCTA
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_018385 unedited
  AACGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTATAAANAAAGAGGTTTA
  ATTGGCTCACGGTCCAGTAGATGGTACAGGAAGCACAGCAGCTTTTGCTTCTAGGGAGGC
  CTCAGGAAACTTACAATCATGTTAGAAGGCGAAGGAGAAGCAGGCACATCTTATATGGCC
  AGAGCAGGAGGGAGACAGAGGGGAGGCACCACACACTTTGAAGCAACCAGATGTGATGAG
  GACTCAATATCAGGAGAACAGCACTGAGCGGGTGGTGCTAAACCGTTTGTGAGGACTCTG
  CCCCATAATCCCATCGCCTCCCACCAGGGGGCTTACATTTCAACATGAGACTCGGTGAGG
  ACACAGATCCAAACCACATCAATAGTGCTTTCATGCTTTTGATTATCTTTTGTAACTATG
  TTATTGAACTATAATTTACATACCATACAATTCACCAACGTAAAGTGTGTAATTCAATGG
  TCTTAAGCATATTCAGAGTTGTGTGACCATCGCTACAGTCAATTTTAGGACATTTTTATC
  ACTGCAAAAGAAAGACCTCAATCTTCCCATTCCTCCCATCCCGAAGCAACCACTAATCTA
  CTTCCTCTATATGGAGATTTGCTTATTCTGGACATTTTAAATAAGTGGAATAACCAGTGA
  AGATGGGGGCTAGGTAGTAAATACCGCAGCTTCCCTGTCACCCGGTGGTTACATTCTACA
  TGTTCCTTAGTGGACGTGAGCCCNCGCTGTACACTAACTGAACCCAGTNCCTTGTTAGAG
  TCGGAGGAACCCNACTAGACACTCAGATGGGGAAATTCTCTTTCACTTCGTCCAAATGTG
  TCTCGTGACCATCCCTGCAATTTTTTTCTTGTGATGCGTCTACTTGCGCGCCTTGGAAGG
  GCAAGGGGCCAATTCCATTACTGCAACTCTGCTCCTGGNNTCAGTGATTCTCTGCCTAGC
  TCCGAGTACTGGATACAGTGCCGCCACCGCTGGCTATTTTTGAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_018385
• Insert Size: 3100 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
• Note: Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required.

Reference Data

• RefSeq: NM_018385.1, NP_060855.1
• RefSeq Size: 3290 bp
• RefSeq ORF: 1977 bp
• Locus ID: 55341
• UniProt ID: Q9H089
• Cytogenetics: 3q29
• Domains: MMR_HSR1
• Gene Summary: This gene encodes a protein related to the yeast large subunit GTPase 1. The encoded protein is necessary for cell viability and may localize in the endoplasmic reticulum, nucleus and cytoplasm.[provided by RefSeq, Feb 2009]